micro1

47問 • 2年前

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What are the different laboratory diagnosis tests for Gram-Positive Anaerobic Spore-Forming Bacilli?

Gram stain Culture Catalase test Direct Nagler test Mouse Neurilization Test Reverse CAMP test Cell Culture Cytoxicity test Lipase and Lethinase test Spot Indole Test

This media is used to observe swarming and double zone of hemolysis; supports nearly all obligate and facultative anaerobes

BRU / BA

This media detects the activity of lipase and lecithinase if its positive (+) there is an insoluble opaque whitish precipitate within the agar

EYA

This media detects volatile fatty acid (Easily evaporated)

PYG

This media is both a selective and differential medium, they inhibit gram-negative coliforms, also a pH indicator (If neutral red turns to yellow means there is a presence of an acid pH.)

CCFA

It is utilized to know the true Gram-staining reaction of the pink-staining anaerobic bacillus

Vancomycin special-potency antimicrobial and aerotolerance test

It has a smooth swarming growth on plated media.

Clostridium septicum

It grows minimally on the aerotolerance plate and can be mistaken for a facultative anaerobe.

Clostridium tertium

-It uses EYA plate and C.perifrigens type A antitoxin -It provides a positive result of (+) if inhibition of lecithinase reaction is produced by Clostridium perfringens

Direct Nagler Test

This test is performed to differentiate the aerotolerant strains of Clostridium (catalase-negative) from Bacillus(catalase-positive)

Catalase test

This test is performed to differentiate C.bifermentans and C. sordellii (indole-positive) from the other clostridia species (indole-negative).

Spot indole test

-It is the gold standard test for the detection of the C. difficile toxin type B. -It takes 2-3 DAYS TO ACHIEVE THE POSITIVE RESULT

Cell Culture Cytotoxicity Test

-This test is used to confirm the C. perfringens -Positive result (+) if there is an arrowhead at the intersection of the 2 streaks (Toward Clostridium isolates)

Reverse CAMP test

-It is a definitive identification test for C. botulinum -It detects the presence of neurotoxins in serum or feces.

Mouse Neutralization test

Gram-positive anaerobic actinomycetes, straight or slightly curved filamentous rods; may be beaded or banded

Actinomyces spp.

Young colonies "spider- like" or wooly appearance Old colonies - "molar tooth" (A. Israelll)

Actinomyces spp.

Gram-negative, pale- staining, pleomorphic rods; resembles "safety pin"

Bacteroides fragilis

Gray-white, circular, smooth, nonhemolytic

Bacteroides fragilis

Gram-negative, pale-Staining; thin rods; some curved

Bacteroides ureolyticus

Colonies corrode (pit) the agar

Bacteroides ureolyticus

Gram-positive diphotheroid; coccold or pointed shape; bifurcated (forked) ends, resemble “dog bones”

Bifidobacterium spp.

Small, white, shiny, convex colonies

Bifidobacterium spp.

Gram-positive rod in young cultures and turns Gram-negative rod with age; spores subterminal

Clostridium septicum

Rhizoid margins resembling "medusa head”

Clostridium septicum

Gram-positive pleomorphic rod, has seagull-wing shape

Eubacterium spp.

Chartreuse color fluorescence

Eubacterium spp.

Gram-negative spindle shaped rod; resembles Capnocytophaga

Fusobacterium nucleatum subsp. Nucleutum

Green-colored medium upon air exposure; “breadcrumb-like” colonies

Fusobacterium nucleatum subsp. Nucleutum

Gram-negative large fusiform rods

Leptotrichia spp.

“Raspberry- like” colonies”

Leptotrichia spp.

Gram negative bacilli which exhibits Brick red fluorescence

Porphyromonas spp.

Gram negative rods which exhibits Brick red fluorescence

Prevotella spp.

Gram negative tiny diplococci which exhibits Red fluorescence

Veillonela parvula

Gram-positive diphtheroid like rod; palisade arrangement

Propionibacterium spp “anaerobic diphtheroids”

It is the most commonly isolated anaerobes from blood cultures. And It is a significant cause of intraabdominal abscesses.

Bacteroides fragilis

Microscopy: pleomorphic with vacuoles; encapsulated

Bacteroides fragilis

Microscopy: thin, nonsporeforming, branching gram-positive bacilli

Actinomyces israelil

Where is the anatomical site of Actinomyces israelil?

Microbiota of the oral cavity

It is a Gram-positive pleomorphic anaerobic nonspore forming rod (coccoid or spiral-shaped), which is also an aerotolerant anaerobe; nonmotile.

Lactobacillus

It has an important role in the health of the female genital tract - it protects the host from urogenital infections.

Lactobacillus acidophilus

It is a chronic, granulomatous, infectious disease characterized by the development of sinus tracts and fistulae, which erupt to the surface and drain pus that may contain sulfur granules" (dense lumps of bacteria that may be colored).

Actinomyces

This infection is usually caused by Lactobacilli

Endocarditis

It is associated with endophthalmitis after cataract surgery.

Propionobacterium acnes

What is the selective medium for the Identification of the Nonsporeforming Anaerobic Bacteria?

Laked kanamycin-vancomycin agar

It is the test utilized to identify B. fragilis (bile-tolerant).

Bile disk test

It is the test that is utilized for rapid identification of Peptostreptococcus anaerobius (sensitive) against Peptostreptococcus assacharolyticus (resistant).

SPS disk

In the Identification of the Nonsporeforming Anaerobic Bacteria, it provides a semisolid agar and anaerobic environment.

PRAS (prereduced and anaerobically sterilized)

Translation(Pre-finals)

MAHATHIR ALPHA · 55問 · 2年前

Translation(Pre-finals)