What are the different types of Culture?

What are the Classification of Culture Media According to Consistency?

What are the Classification of Culture Media According to Composition?

What are the Classification of Culture Media According to Dispensing or Distribution method for medium?

Type of media which are distributed into the dish or plate

Type of media which are prepared as either liquid, slant, butt and slant, or butt.

What are the Classification of Culture Media According to Use?

What are the 3 types of hemolysis?

► Mixture of nutrients such as carbon, nitrogen, sulphur, phosphorus, hydrogen, oxygen, and buffers. ► Inhibitory agents like dyes, salts, and antimicrobial added to facilitate the isolation of the desired organism while suppressing the growth of other organisms present in the sample.

Type of culture which is composed of only one specie.

Type of culture which composed of more than one species

Type of culture which composed of several species contained in a separate culture medium. Should be grown in large volume of broth and divide into small freezer vials.

-Does not contain any amount of agar -Allows the growth of aerobes, anaerobes and facultative anaerobes.

-contains 0.5% to 1% agar - used to observe bacterial motility, detect indole and sulfide production

Contains 2% to 3% agar

-Type of culture media wherein components are known. - Used for research purposes as either liquid or solid medium -Preferred for the isolation of cyanobacteria and chemo organotrophs

- substances are unknown (peptones, meat, and yeast extracts) -very useful for isolation of medically significant bacteria

This type of media is used for obligate intracellular bacteria (Rickettsia and Chlamydia).

-It is routinely used in the laboratory and without addtional supplements. -Support the growth of most non-fastidious bacteria -Usually composed of meat and soybean extract

-It is used to propagate the growth of a certain group of bacteria from a mixture of organisms. -contains specific nutrients and without additional supplements -can also be used as a supplement to agar plates to detect aerobes, anaerobes and microaerophiles (thioglycollate)

-With additional supplements such as blood, vitamins and yeast extract, which are necessary for the growth of fastidious organisms -These are solid type media

It differentiate the haemolytic pattern of bacteria

It is used for the recovery of Haemophilus species

They are used for isolation of fastidious organisms

It contains general nutrients with 5% - 10% blood

It shows complete lysis of red cells resulting in complete clearing around the colonies.

Incomplete lysis of red cells, producing a greenish discoloration of the blood agar around colonies.

Shows no hemolysis, resulting in no change in the medium

It allows the visualization of metabolic differences between groups of bacteria

What is the pH indicator in MAC that detects the fermentation of sugar and eventually the production of acid?

Incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the growth of desired organism.

What are the types of selective media?

Isolation of fungi

It contains antibiotics trimethoprim, nystatin, vancomycin, and colistin. It is used for the isolation of Neisseria.

It contains 10% NaCl and used for the isolation of Staphylococcus aureus.

It promotes growth of gram-negative bacteria, primarily belongs to the family Enterobacteriaceae. Inhibit the growth of gram-positive bacteria through the addition of bile salts.

It is used to recover Mycobacterium tuberculosis.

It is used to isolate bacteria with specific growth requirements

It is made up of whole egg and malachite green which supports the growth of mycobacteria.

It is used for isolation of Vibrio