Culture Media

MAHATHIR ALPHA · 55問 · 2年前

通報

問題一覧

What are the different types of Culture?

Pure culture, Mixed culture, Stock culture

What are the Classification of Culture Media According to Consistency?

Liquid medium, Semi-solid medium, Solid medium

What are the Classification of Culture Media According to Composition?

Synthetic or defined medium, Non-synthetic or complex medium, Tissue culture medium

What are the Classification of Culture Media According to Dispensing or Distribution method for medium?

Plated media, Tube media

Type of media which are distributed into the dish or plate

Plated media

Type of media which are prepared as either liquid, slant, butt and slant, or butt.

Tube media

What are the Classification of Culture Media According to Use?

Simple media, Enrichment media, Enriched and non-selective media, Differential media, Selective media, Special media

What are the 3 types of hemolysis?

Alpha hemolysis, Beta hemolysis, Gamma hemolysis

► Mixture of nutrients such as carbon, nitrogen, sulphur, phosphorus, hydrogen, oxygen, and buffers. ► Inhibitory agents like dyes, salts, and antimicrobial added to facilitate the isolation of the desired organism while suppressing the growth of other organisms present in the sample.

Culture Media

Type of culture which is composed of only one specie.

Pure culture

Type of culture which composed of more than one species

Mixed culture

Type of culture which composed of several species contained in a separate culture medium. Should be grown in large volume of broth and divide into small freezer vials.

Stock culture

-Does not contain any amount of agar -Allows the growth of aerobes, anaerobes and facultative anaerobes.

Liquid medium

-contains 0.5% to 1% agar - used to observe bacterial motility, detect indole and sulfide production

Semi-solid medium

Contains 2% to 3% agar

Solid medium

-Type of culture media wherein components are known. - Used for research purposes as either liquid or solid medium -Preferred for the isolation of cyanobacteria and chemo organotrophs

Synthetic or defined medium

- substances are unknown (peptones, meat, and yeast extracts) -very useful for isolation of medically significant bacteria

Non-synthetic or complex medium

This type of media is used for obligate intracellular bacteria (Rickettsia and Chlamydia).

Tissue culture medium

-It is routinely used in the laboratory and without addtional supplements. -Support the growth of most non-fastidious bacteria -Usually composed of meat and soybean extract

Simple media

-It is used to propagate the growth of a certain group of bacteria from a mixture of organisms. -contains specific nutrients and without additional supplements -can also be used as a supplement to agar plates to detect aerobes, anaerobes and microaerophiles (thioglycollate)

Enrichment media

-With additional supplements such as blood, vitamins and yeast extract, which are necessary for the growth of fastidious organisms -These are solid type media

Enriched media and non-selective media

It differentiate the haemolytic pattern of bacteria

BAP

It is used for the recovery of Haemophilus species

CAP

They are used for isolation of fastidious organisms

BAP and CAP

It contains general nutrients with 5% - 10% blood

Blood agar

It shows complete lysis of red cells resulting in complete clearing around the colonies.

Beta hemolysis

Incomplete lysis of red cells, producing a greenish discoloration of the blood agar around colonies.

Alpha hemolysis

Shows no hemolysis, resulting in no change in the medium

Gamma hemolysis

It allows the visualization of metabolic differences between groups of bacteria

Differential media

What is the pH indicator in MAC that detects the fermentation of sugar and eventually the production of acid?

Neutral red

Incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the growth of desired organism.

Selective media

What are the types of selective media?

Thayar- Martin agar, Mannitol Salt agar, MacConkey's agar, Lowenstein- Jensen Medium, Sabouraud's dextrose agar

Isolation of fungi

Sabouraud's dextrose agar

It contains antibiotics trimethoprim, nystatin, vancomycin, and colistin. It is used for the isolation of Neisseria.

Thayer- Martin agar

It contains 10% NaCl and used for the isolation of Staphylococcus aureus.

Mannitol salt agar

It promotes growth of gram-negative bacteria, primarily belongs to the family Enterobacteriaceae. Inhibit the growth of gram-positive bacteria through the addition of bile salts.

MacConkey's agar

It is used to recover Mycobacterium tuberculosis.

Lowenstein-Jensen medium

It is used to isolate bacteria with specific growth requirements

Special media

It is made up of whole egg and malachite green which supports the growth of mycobacteria.

Lowenstein- Jensen (LJ)

It is used for isolation of Vibrio

TCBS

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