Cell Wall-deficient Bacteria

41問 • 2年前

問題一覧

-It considered as the smallest free-living organisms that are capable of growing in artificial media. -Pleomorphic organisms that lack a cell wall. -Found mainly in the oropharyngeal, upper respiratory, and genitourinary tracts. -Slow-growing, fastidious, and facultative anaerobes that replicate by binary fission.

Mycoplasma and Ureaplasma (Mollicutes)

Mycoplasma and Ureaplasma (Mollicutes) require _____________ for membrane function and growth.

sterols (cholesterols)

Mycoplasma and Ureaplasma (Mollicutes) are common parasites of the _________ and their transmission is related to ___________.

genital tract sexual activity

What are the species of Mycoplasma and Ureaplasma (Mollicutes)?

Mycoplasma pneumoniae Mycoplasma hominis Mycoplasma fermentans Mycoplasma pirum Mycoplasma penetrans Ureaplasma urealyticum

Etiologic agent of primary atypical pneumonia or walking pneumonia. And is not included in the normal commensals and it is extremely fastidious.

Mycoplasma pneumoniae

What is the mode of acquisition of Mycoplasma pneumoniae?

Inhalation of contaminated aerosol droplets

How is disease caused by Mycoplasma pneumoniae initiated?

Attachment to respiratory mucosal cells, evasion from phagocytosis, and modulation of the immune system

What is used for the culture of Mycoplasma pneumoniae?

SP4 broth

Describe Mycoplasma pneumoniae in SP4 broth

Colonies exhibit a yellow color

Species can be recovered from the genital tract of healthy adults

Mycoplasma hominis and Ureaplasma urealyticum (Genital Mollicutes)

They are also termed as Genital Mollicutes

Mycoplasma hominis Ureaplasma urealytocum

Mycoplamsa hominis and Ureaplasma urealyticum (Genital Mollicutes) causes which disease/infection?

Prostatis Pelvic inflammatory disease

What does Mycoplamsa hominis cause?

Post-abortal fever and Postpartum fever in women

Colonies exhibit a “fried egg” appearance on a plated medium.

Mycobacterium hominis

Colonies exhibit dark-brownish lumps on an A7 or A8 agar medium.

Ureaplasma urealyticum

What are the specimens uses for the laboratory diagnosis of Mycoplasma pneumoniae?

Throat swab Serum Bronchoalveolar lavage Sputum Lung tissue

What are the specimens uses for the laboratory diagnosis of Genital mycoplasma?

Urethral, Vaginal or endocervical swab Blood Urine Prostatic secretions Semen

True/False: There is no direct method or Gram staining can be used for identification of Cell wall -deficient bacteria

True

What are the media used in the culture of Cell wall -deficient bacteria?

Beef or soybean protein with serum Fresh yeast extract Biphasic SP4 medium Pleuropneumonia-like organism (PPLO) broth or agar with yeast extract and horse serum A8 agar Shepard’s 10B arginine broth Modified New York City medium.

What are the swabs used for sample collection of Cell wall -deficient bacteria?

Calcium alginate Dacron swabs

What are the transport medium used for Cell wall -deficient bacteria?

SP4 (sucrose phosphate buffer, horse serum, and neutral red)

True/False: Mycoplasma and Ureaplasma grow slowly than most of the other bacteria

True

It is the only specie of Mycoplasma and Ureaplasma (Mollicutes) that is capable of growing on BAP and CAP.

Mycoplasma hominis

It requires a biphasic culture system and incubation of up to three weeks in a chamber with 5% to 10% CO2.

Mycoplasma pneumoniae

What can be incorporated into the media to detect the presence of U. urealyticum and M. hominis and produce an alkaline reaction?

Urea and/or arginine

Growth of mycoplasmas in Shepard’s 10B arginine medium is exhibited as ___________.

red colorations

What is not recommended for mycoplasmas?

Blood culture

Overlaying suspicious colonies with 0.1% guinea pig RBC in PO4 buffered saline is the definitive identification method of __________________.

Mycoplasma pneumoniae

It is the most commonly used serological method for diagnosis of Mycoplasma and Ureaplasma.

ELISA

What are the different test used in the laboratory diagnosis of Cell wall-deficient bacteria?

Gram stain Culture media Serodiagnosis Manganese chloride urea test

The detection of specific IgM in a single serum sample is diagnostic of the acute infection that is caused by _________________.

Mycoplamsa pneumoniae

It is the rapid identification test for U. urealyticum; the reaction for the test is observed under a dissecting microscope.

Manganese chloride urea test

What is the positive result for manganese chloride urea test?

Dark brown precipitate of manganese oxide around the colonies

They utilizes manganese chloride in the presence of urea.

Ureaplasma urealyticum

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards 'Obligate intracellular'.

Bacteria(-) Rickettsieae(+) Chlamydiae(+) Mycoplasmas(-)

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards with having 'DNA and RNA'

Bacteria(+) Rickettsieae(+) Chlamydiae(+) Mycoplasmas(+)

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards with having ribosomes

Bacteria(+) Rickettsieae(+) Chlamydiae(+) Mycoplasmas(+)

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards with having Peptidoglycan in the cell wall

Bacteria(+) Rickettsieae(-) Chlamydiae(+) Mycoplasmas(-)

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards with Replication by binary fission

Bacteria(+) Rickettsieae(+) Chlamydiae(+) Mycoplasmas(+)

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards with its Growth on non-living media

Bacteria(+) Rickettsieae(-) Chlamydiae(-) Mycoplasmas(+)

In the comparison between common bacteria and atypical bacteria, what are positive and negative in regards with its sensitivity with antibiotics

Bacteria(+) Rickettsieae(+) Chlamydiae(+) Mycoplasmas(+)

Translation(Pre-finals)

MAHATHIR ALPHA · 55問 · 2年前

Translation(Pre-finals)