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1
It is also known as Comma-shape or Curved bacillus
Vibrio
2
Not a human microbiota, facultatively anaerobic and monotrichous organisms.
Vibrio
3
Where can Vibrio be found?
Brackish water Marine water Salt water
4
Vibrio can be isolated from what?
Algae Plankton Fish Shellfish
5
Vibrio are halophilic organisms except for ___________________.
V. cholerae and V. mimicus
6
What is the mode of acquisition of Vibrio?
Consumption of raw or undercooked seafood
7
What are the diseases/infections that can be caused by Vibrio?
Cholera Wound infection Septicemia Necrotizing facitiis
8
What are the common isolates of Vibrio?
Vibrio cholerae Vibrio parahaemolyticus Vibrio vulnificus Vibrio alginolyticus
9
Describe the appearance of Vibrio under the microscope
Gram-negative, short, curved, asporogenous rods.
10
It is the causative agent of cholera
Vibrio cholerae
11
What is the common cause of cholera?
Vibrio cholera O1
12
It is an acute diarrheal infection that is mainly spread through contaminated water sources.
Cholera
13
What is the hallmark of cholera?
Rice-watery stool (10-30 times of defecation per day)
14
V. cholerae O1 caused ____________, while non-O1 strain causes ____________.
Epidemic cholera Gastroenteritis
15
It is a protein toxin produced mainly by Vibrio cholerae O1 strain
Choleragen
16
Describe the motility of Vibrio cholerae
Has a rapid darting or “ shooting star” motility
17
Second most common Vibrio species that is associated with gastroenteritis
Vibrio parahaemolyticus
18
It was the etiologic agent of the outbreak of “summer diarrhea” in Japan in 1950
Vibrio parahaemolyticus
19
What is the selective medium for Vibrio parahaemolyticus?
Wagatsuma agar
20
Known as the “ lactose-positive” Vibrio species
Vibrio vulnificus
21
Second to V. cholerae as the cause of a severe Vibrio-associated infections.
Vibrio vulnificus
22
What are the infections caused by Vibrio vulnificus?
Septicemia Wound infection
23
Vibrio species that is commonly isolated and is considered as the least pathogenic to humans
Vibrio alginolyticus
24
Vibrio alginolyticus is a ____________ that requires a medium with 1% to 10% NaCl.
strict halophile
25
What are infections caused by Vibrio alginolyticus?
Eye infection Ear infection Wound infection
26
What are the laboratory test for Vibrio?
Gram stain Culture String test Vibriostatic test Biochemical test
27
What is the transport medium for the culture of vibrio?
Cary-Blair medium
28
What is the Enrichment media for V.cholerae?
Alkaline peptone water ( pH 8.5)
29
What are the Culture media used in the Laboratory diagnosis of Vibrio?
MAC CAP TCBS agar Alkaline peptone water
30
-Species are found in fresh, estuarine, and chlorinated water -Not part of human microbiota and glucose fermenters
Aeromonas
31
-Motile with single polar flagellum and facultatively anaerobic -May cause traveller’s diarrhea similar to ETEC
Aeromonas
32
It is the causative agent of “red leg” disease in amphibians
Aeromonas
33
What are the human infections caused by aeromonas?
Wound infection Septicemia Meningitis Keratitis
34
Give out atleast 3 species of Campylobacter
Campylobacter jejuni Campylobacter coli Campylobacter lari Campylobacter fetus Campylobacter sputorum
35
Give out the Enteric CAMPYLOBACTER
Campylobacter jejuni Campylobacter coli Campylobacter lari
36
What is the mode of acquision of Campylobacter?
Ingestion of poultry and dairy products and sexual transmission
37
What is the optimum temperature for the growth of Campylobacter jejuni?
It is 42 degree celsius
38
What is the motility of Campylobacter jejuni?
Darting motility
39
-They are slow-growing, fastidious and asaccharolytic organism -Has darting motility and is unable to grow with high salt concentration.
Campylobacter jejuni
40
Campylobacter jejuni is the most common cause of what?
Bacterial gastroenteritis
41
-Most common cause of bacterial gastroenteritis -Causes septic arthritis among AIDS patients
Campylobacter jejuni
42
This specie of Campylobacter causes bacteremia and is rarely associated with gastrointestinal illness
Campylobacter fetus
43
What are the specimens used in the laboratory diagnosis of Campylobacter?
Feces Rectal swab Blood
44
What are the Laboratory Diagnosis for Campylobacter?
Microscopy Culture
45
What is the recommended counterstain for the microscopy of Campylobacter?
Carbolfuchsin
46
Result of Hanging drop preparation in the laboratory diagnosis of Campylobacter
Exhibits a darting motility
47
What are the selective media used in in the culture of Campylobacter?
Campy-BAP Butzler agar Skirrow's medium CCDA
48
What is the transport medium used in the culture of Campylobacter?
Cary-Blair medium
49
Describe the flagella of Campylobacter
Single polar flagellum
50
Describe the flagella of Helicobacter
Monopolar or multi-bipolar flagella
51
Describe Helicobacter under the microscope
Gram-negative, helical (S- shaped) rods that resemble campylobacter
52
What is the routes of transmission of Helicobacter?
Oral-oral route and Fecal-oral route
53
Give out atleast 3 species of Helicobacter
Helicobacter pylori Helicobacter cinaedi Helicobacte fenneliae
54
They are found in the mucous layer of the antrum and fundus of the stomach but does not penetrate the gastric epithelium
Helicobacter pylori
55
What does Helicobacter pylori bind with?
Lewis antigen Monosaccharide sialic acid
56
What is the primary habitat of Helicobacter pylori?
Human gastric mucosa
57
-Major cause of type B gastritis, peptic ulcer, and gastric carcinoma -Found in the mucous layer of the antrum and fundus of the stomach but does not penetrate the gastric epithelium
Helicobacter pylori
58
These species have been isolated from the blood of patients with bacteremia and recovered from blood of homosexual males with or without HIV
Helicobacter cinaedi Helicobacter fenneliae
59
What are the specimens used in the laboratory diagnosis of Helicobacter?
Gastric biopsy tissue Urine Feces Dental plaque
60
What is the best specimen for the culture of H. pylori?
Gastric tissue
61
True/False: Gastric tissue in the culture of Helicobacter pylori should be maintained at 4 deg.cent and processed within 2 hours of collection
True
62
In the laboratory diagnosis of helicobacter, what is utilized for ammonia testing?
Urine specimen
63
What are the laboratory diagnosis for Helicobacter?
Gram stain Culture
64
What are the stains for biopsy specimen in Helicobacter?
Warthin-starry stain Giemsa
65
True/False: In the gram stain of Helicobacter, 0.1% basic fuchsin counterstain DOES NOT enhances morphology
False
66
Helicobacter pylori is susceptible to what?
Metronidazole
67
What are the other tests for Helicobacter aside from culture and gram stain?
Nucleic amplification Susceptibility test Urea breath test
68
Agar dilution using MHA with 5% sheep’s blood incubated at microaerophilic condition and read after 3 days
Susceptibility test
69
It is the sensitive method of detecting H. pylori
Nucleic amplification
70
What are the transport medium for the culture of Helicobacter?
Stuart medium Cysteine brucella broth with 20% glycerol Isotonic saline with 4% glucose
71
In the culture of Helicobacter, how long should the incubation be in a capnophilic environment?
Helicobacters may require more than five days of incubation in a capnophilic environment.
72
What are the selective media used in Helicobacter?
BAP MTM Skirrow's agar Brucella agar with 5% sheep's blood