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Non-enteric gastrointesinal Pathogens
  • MAHATHIR ALPHA

  • 問題数 72 • 12/13/2023

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  • 1

    It is also known as Comma-shape or Curved bacillus

    Vibrio

  • 2

    Not a human microbiota, facultatively anaerobic and monotrichous organisms.

    Vibrio

  • 3

    Where can Vibrio be found?

    Brackish water Marine water Salt water

  • 4

    Vibrio can be isolated from what?

    Algae Plankton Fish Shellfish

  • 5

    Vibrio are halophilic organisms except for ___________________.

    V. cholerae and V. mimicus

  • 6

    What is the mode of acquisition of Vibrio?

    Consumption of raw or undercooked seafood

  • 7

    What are the diseases/infections that can be caused by Vibrio?

    Cholera Wound infection Septicemia Necrotizing facitiis

  • 8

    What are the common isolates of Vibrio?

    Vibrio cholerae Vibrio parahaemolyticus Vibrio vulnificus Vibrio alginolyticus

  • 9

    Describe the appearance of Vibrio under the microscope

    Gram-negative, short, curved, asporogenous rods.

  • 10

    It is the causative agent of cholera

    Vibrio cholerae

  • 11

    What is the common cause of cholera?

    Vibrio cholera O1

  • 12

    It is an acute diarrheal infection that is mainly spread through contaminated water sources.

    Cholera

  • 13

    What is the hallmark of cholera?

    Rice-watery stool (10-30 times of defecation per day)

  • 14

    V. cholerae O1 caused ____________, while non-O1 strain causes ____________.

    Epidemic cholera Gastroenteritis

  • 15

    It is a protein toxin produced mainly by Vibrio cholerae O1 strain

    Choleragen

  • 16

    Describe the motility of Vibrio cholerae

    Has a rapid darting or “ shooting star” motility

  • 17

    Second most common Vibrio species that is associated with gastroenteritis

    Vibrio parahaemolyticus

  • 18

    It was the etiologic agent of the outbreak of “summer diarrhea” in Japan in 1950

    Vibrio parahaemolyticus

  • 19

    What is the selective medium for Vibrio parahaemolyticus?

    Wagatsuma agar

  • 20

    Known as the “ lactose-positive” Vibrio species

    Vibrio vulnificus

  • 21

    Second to V. cholerae as the cause of a severe Vibrio-associated infections.

    Vibrio vulnificus

  • 22

    What are the infections caused by Vibrio vulnificus?

    Septicemia Wound infection

  • 23

    Vibrio species that is commonly isolated and is considered as the least pathogenic to humans

    Vibrio alginolyticus

  • 24

    Vibrio alginolyticus is a ____________ that requires a medium with 1% to 10% NaCl.

    strict halophile

  • 25

    What are infections caused by Vibrio alginolyticus?

    Eye infection Ear infection Wound infection

  • 26

    What are the laboratory test for Vibrio?

    Gram stain Culture String test Vibriostatic test Biochemical test

  • 27

    What is the transport medium for the culture of vibrio?

    Cary-Blair medium

  • 28

    What is the Enrichment media for V.cholerae?

    Alkaline peptone water ( pH 8.5)

  • 29

    What are the Culture media used in the Laboratory diagnosis of Vibrio?

    MAC CAP TCBS agar Alkaline peptone water

  • 30

    -Species are found in fresh, estuarine, and chlorinated water -Not part of human microbiota and glucose fermenters

    Aeromonas

  • 31

    -Motile with single polar flagellum and facultatively anaerobic -May cause traveller’s diarrhea similar to ETEC

    Aeromonas

  • 32

    It is the causative agent of “red leg” disease in amphibians

    Aeromonas

  • 33

    What are the human infections caused by aeromonas?

    Wound infection Septicemia Meningitis Keratitis

  • 34

    Give out atleast 3 species of Campylobacter

    Campylobacter jejuni Campylobacter coli Campylobacter lari Campylobacter fetus Campylobacter sputorum

  • 35

    Give out the Enteric CAMPYLOBACTER

    Campylobacter jejuni Campylobacter coli Campylobacter lari

  • 36

    What is the mode of acquision of Campylobacter?

    Ingestion of poultry and dairy products and sexual transmission

  • 37

    What is the optimum temperature for the growth of Campylobacter jejuni?

    It is 42 degree celsius

  • 38

    What is the motility of Campylobacter jejuni?

    Darting motility

  • 39

    -They are slow-growing, fastidious and asaccharolytic organism -Has darting motility and is unable to grow with high salt concentration.

    Campylobacter jejuni

  • 40

    Campylobacter jejuni is the most common cause of what?

    Bacterial gastroenteritis

  • 41

    -Most common cause of bacterial gastroenteritis -Causes septic arthritis among AIDS patients

    Campylobacter jejuni

  • 42

    This specie of Campylobacter causes bacteremia and is rarely associated with gastrointestinal illness

    Campylobacter fetus

  • 43

    What are the specimens used in the laboratory diagnosis of Campylobacter?

    Feces Rectal swab Blood

  • 44

    What are the Laboratory Diagnosis for Campylobacter?

    Microscopy Culture

  • 45

    What is the recommended counterstain for the microscopy of Campylobacter?

    Carbolfuchsin

  • 46

    Result of Hanging drop preparation in the laboratory diagnosis of Campylobacter

    Exhibits a darting motility

  • 47

    What are the selective media used in in the culture of Campylobacter?

    Campy-BAP Butzler agar Skirrow's medium CCDA

  • 48

    What is the transport medium used in the culture of Campylobacter?

    Cary-Blair medium

  • 49

    Describe the flagella of Campylobacter

    Single polar flagellum

  • 50

    Describe the flagella of Helicobacter

    Monopolar or multi-bipolar flagella

  • 51

    Describe Helicobacter under the microscope

    Gram-negative, helical (S- shaped) rods that resemble campylobacter

  • 52

    What is the routes of transmission of Helicobacter?

    Oral-oral route and Fecal-oral route

  • 53

    Give out atleast 3 species of Helicobacter

    Helicobacter pylori Helicobacter cinaedi Helicobacte fenneliae

  • 54

    They are found in the mucous layer of the antrum and fundus of the stomach but does not penetrate the gastric epithelium

    Helicobacter pylori

  • 55

    What does Helicobacter pylori bind with?

    Lewis antigen Monosaccharide sialic acid

  • 56

    What is the primary habitat of Helicobacter pylori?

    Human gastric mucosa

  • 57

    -Major cause of type B gastritis, peptic ulcer, and gastric carcinoma -Found in the mucous layer of the antrum and fundus of the stomach but does not penetrate the gastric epithelium

    Helicobacter pylori

  • 58

    These species have been isolated from the blood of patients with bacteremia and recovered from blood of homosexual males with or without HIV

    Helicobacter cinaedi Helicobacter fenneliae

  • 59

    What are the specimens used in the laboratory diagnosis of Helicobacter?

    Gastric biopsy tissue Urine Feces Dental plaque

  • 60

    What is the best specimen for the culture of H. pylori?

    Gastric tissue

  • 61

    True/False: Gastric tissue in the culture of Helicobacter pylori should be maintained at 4 deg.cent and processed within 2 hours of collection

    True

  • 62

    In the laboratory diagnosis of helicobacter, what is utilized for ammonia testing?

    Urine specimen

  • 63

    What are the laboratory diagnosis for Helicobacter?

    Gram stain Culture

  • 64

    What are the stains for biopsy specimen in Helicobacter?

    Warthin-starry stain Giemsa

  • 65

    True/False: In the gram stain of Helicobacter, 0.1% basic fuchsin counterstain DOES NOT enhances morphology

    False

  • 66

    Helicobacter pylori is susceptible to what?

    Metronidazole

  • 67

    What are the other tests for Helicobacter aside from culture and gram stain?

    Nucleic amplification Susceptibility test Urea breath test

  • 68

    Agar dilution using MHA with 5% sheep’s blood incubated at microaerophilic condition and read after 3 days

    Susceptibility test

  • 69

    It is the sensitive method of detecting H. pylori

    Nucleic amplification

  • 70

    What are the transport medium for the culture of Helicobacter?

    Stuart medium Cysteine brucella broth with 20% glycerol Isotonic saline with 4% glucose

  • 71

    In the culture of Helicobacter, how long should the incubation be in a capnophilic environment?

    Helicobacters may require more than five days of incubation in a capnophilic environment.

  • 72

    What are the selective media used in Helicobacter?

    BAP MTM Skirrow's agar Brucella agar with 5% sheep's blood