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Staphylococcus Part 1
  • MAHATHIR ALPHA

  • 問題数 80 • 11/15/2023

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  • 1

    What are the Enzymes and Toxins produced by Staphylococcus aureus?

    Coagulase, Hyaluronidase, Staphylokinase, Lipase, Deoxyribonuclease(Dnase) and phosphatase, B-lactamase, Enterotoxin, Leukocidin, Hemolysin, Exfoliatin serotypes A and B, Toxic shock syndrome toxin, Protein A

  • 2

    What are the diagnosis for Osteomyelitis

    X-ray, MRI, Bone aspirates

  • 3

    They only grow aerobically (strict aerobes).

    Micrococcus

  • 4

    True/False: Micrococcus is Microdase positive

    True

  • 5

    Phylum of Staphylococci

    Firmicutes

  • 6

    It is used to differentiate the pathogenic staph. aureus From non-pathogenic ones

    Mannitol fermentation test

  • 7

    Staphylocci which is Coagulase positive

    Staphylococcus aureus, Staphylococcus intermedius, Staphylococcus pseudointermedius, Staphylococcus hyicus, Staphylococcus delphini, Staphylococcus lutrae, Staphylococcus agnetis, Staphylococcus scheliferi(some strain)

  • 8

    True/False: Staphylococcus is resistant to 0.04 U of bacitracin.

    True

  • 9

    What is the original colour of VP test?

    Colorless

  • 10

    What are the specimens used in the Laboratory Diagnosis of Staphylococci?

    Aspirated secretion (best sample), Purulent exudates, Joint fluids

  • 11

    Also known as lemon yellow colonies

    Staphylococcus citreus

  • 12

    True/False: Micrococcus is not susceptible to 0.04 U of bacitracin

    False

  • 13

    It coagulates the fibrinogen in the plasma. Promotes the formation of a fibrin layer around the staphylococci abscess thereby protecting the bacteria from phagocytosis.

    Coagulase

  • 14

    It is bound to the cell wall and clots human, rabbit, or pig plasma by directly converting fibrinogen into fibrin.

    Cell-bound coagulase or clumping factor

  • 15

    They are Catalase-producing and facultatively anaerobic except for S. saccharolyticus.

    Staphylococci

  • 16

    How can bacteria get to the bone?

    Via bloodstream, Following an injury

  • 17

    The exception in staphylococci because it is an obligate anaerobe

    Staphylococcus saccharolyticus

  • 18

    What is the positive result for Tube method?

    Clot or coagulation formation after one to four hours of incubation

  • 19

    What are the two method that can be used in Coagulase test?

    Slide method, Tube method

  • 20

    True/False: Staphylococcus is fermentative

    True

  • 21

    It is considered sensitive but definitive method, detects extracellular or free coagulase.

    Tube method

  • 22

    It is the other important of the cell wall aside the peptidoglycan layer.

    Teichoic acid

  • 23

    What are the different types of Hemolysin?

    Alpha-hemolysin, Beta-hemolysin, Delta-hemolysin, Gamma-hemolysin

  • 24

    This family is consist of Gram positive cocci, arranged in tetrads, clusters

    Micrococcaceae

  • 25

    Where does S. aureus grow?

    Ordinary media

  • 26

    True/False: Staphylococcus is microdase positive.

    False

  • 27

    Also known as golden yellow colonies

    Staphylococcus aureus

  • 28

    He was the first to see staphylococci in pus specimen in 1878.

    Robert Koch

  • 29

    Staphylcocci have ___ species and ___ sub species.

    32, 15

  • 30

    It is an extensive exfoliative dermatitis that occurs primarily in newborns and previously healthy children.

    Scalded skin syndrome

  • 31

    It is a very superficial skin infection common in children, usually produces blisters or sores on the face, neck, hands and diaper area. It is characterized by watery bristles, which become pustules and then honey colouted crust.

    Impetigo

  • 32

    It breaks down penicillin and other B-lactam drugs. More than 90% of clinical staphylococci isolates are penicillin-resistant as a result of enzyme production.

    B-lactamase

  • 33

    Staphylococci are gram positive cocci which is classified into these 2 families:

    Micrococcaceae, Streptococcaceae

  • 34

    It is the major structural component of the cell wall. It is important in the pathogenesis of staphylococcal infections.

    The peptidoglycan layer

  • 35

    What are the 4 genera of Micrococcaceae?

    Planococcus, Micrococcus, Stomatococcus, Staphylococcus

  • 36

    What does Staphylococcus aureus and S. lugdunensis have in common which makes it possible to confuse it with each other?

    mecA gene

  • 37

    Greek word 'Kokkus' means

    berry

  • 38

    Type of Coagulase

    Cell-bound coagulase or clumping factor, Unbound or free coagulase

  • 39

    Who first cultured in liquid media?

    Louis Pasteur

  • 40

    Who first observed in pus in 1871?

    Friedrich von Recklinghausen

  • 41

    He named S. aureus and S. albus in 1884

    Rosenbach

  • 42

    What does CONS stands for?

    Coagulase negative staphylococcus

  • 43

    What is the positive result for Mannitol fermentation test?

    Yellow coloured S.aureus colonies

  • 44

    True/False: Staphylococcus is not susceptible to antibiotic furazolidone.

    False

  • 45

    It is used to screen catalase positive colonies, detects cell bound coagulase or clumping factor.

    Slide method

  • 46

    It attacks and kills white blood cells, pore forming exotoxin that suppresses phagocytosis and responsible for necrotizing skin and soft tissue infections.

    Leukocidin/Panton-Valentine leukocidin (Cytolytic toxin)

  • 47

    He named the bacteria as "staphylococcus" in 1881

    Sir Alexander Ongston

  • 48

    It is an extracellular enzyme that is not bound to the cell wall and causes clot formation when bacterial cells are incubated with plasma.

    Unbound or free coagulase

  • 49

    It is an aggregation of infected furuncles. it may form large abscesses. It is a large are of redness, swelling and pain, punctuated by several sites of drainage pus.

    Carbuncle

  • 50

    Other slide coagulase Staphylococcus are:

    S. lugdunensis, S. schleiferi

  • 51

    Most commonly used method of Coagulase test

    Tube method

  • 52

    Species of Staphylococcus that are causes of endocarditis

    Staphylococcus lugdunensis, Staphylococcus aureus

  • 53

    A method of Coagulase test that is not used nowadays

    Slide method

  • 54

    What are the differential tests for Staphylococcus aureus?

    Coagulase test, Mannitol fermentation test, Tellurite glycine agar, Polymyxin sensitivity test, Voges-Proskauer (VP) test, Deoxyribonuclease (Dnase) test

  • 55

    Specie of Staphylococcus that is a cause of urinary tract infection

    Staphylococcus saprophyticus

  • 56

    It refers to the inflammation of the bone

    Osteomyelitis

  • 57

    It is a folliculitis affecting one or more hair follicles on the edge of the upper or lower eyelid.

    Sty

  • 58

    It is less toxic than a and B-lysins, produced by all S.aureus strains that cause RBC injury in culture and produces edematous lesions.

    Gamma-hemolysin

  • 59

    It destroys RBC and is associated with the Panton-Valentine leukocidin.

    Delta-hemolycin

  • 60

    Staphylocci which are Coagulase negative

    S. epidermidis, S. hemolyticus, S. saprophyticus, S. hominis, S. capitis, S. schleiferi, S. simulans, S. warneri

  • 61

    It causes honeymoon cystitis

    Staphylococcus saprophyticus

  • 62

    What is the pH indicator for Mannitol fermentation test?

    Phenol red

  • 63

    A unique property of _________ is its ability to bind to the Fc part of all IgG molecules except IgG3.

    Protein A

  • 64

    It is a chromosomal-mediated toxin and causes almost all cases of menstruation-associated TSS and it stimulates the production of a large amount of cytokines that are responsible for the symptoms.

    Toxic shock syndrome toxin 1 (TSST-1)/ Enterotoxin F/Pyogenic exotoxin

  • 65

    True/False: Micrococcus is resistant to antibiotic furazolidone.

    True

  • 66

    True/False: Micrococcus utilize sugars oxidatively or not at all in the O/F test.

    True

  • 67

    It enhances invasion and survival in the tissue. Breaks down the hyaluronic acid that is present in the intracellular ground substances of connective tissues, resulting in the spread of bacteria.

    Hyaluronidase (Spreading-factor enzyme)

  • 68

    Species of Staphylococcus which are causes for nosocomial infection

    Staphylococcus epidermidis, Staphylococcus hemolyticus

  • 69

    What is the Family of Staphylococcus?

    Micrococcaceae

  • 70

    True/False: Micrococcus is lysed with lysostaphin (sensitive).

    False

  • 71

    It is a serin protease that divides the intracellular bridges of the epidermidis and causes extensive sloughing of the epidermis to produce a burn-like effect on the patient.

    Exfoliatin serotypes A and B (Superantigens)/ Epidermolytic toxins A and B