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1
What are the Enzymes and Toxins produced by Staphylococcus aureus?
Coagulase, Hyaluronidase, Staphylokinase, Lipase, Deoxyribonuclease(Dnase) and phosphatase, B-lactamase, Enterotoxin, Leukocidin, Hemolysin, Exfoliatin serotypes A and B, Toxic shock syndrome toxin, Protein A
2
What are the diagnosis for Osteomyelitis
X-ray, MRI, Bone aspirates
3
They only grow aerobically (strict aerobes).
Micrococcus
4
True/False: Micrococcus is Microdase positive
True
5
Phylum of Staphylococci
Firmicutes
6
It is used to differentiate the pathogenic staph. aureus From non-pathogenic ones
Mannitol fermentation test
7
Staphylocci which is Coagulase positive
Staphylococcus aureus, Staphylococcus intermedius, Staphylococcus pseudointermedius, Staphylococcus hyicus, Staphylococcus delphini, Staphylococcus lutrae, Staphylococcus agnetis, Staphylococcus scheliferi(some strain)
8
True/False: Staphylococcus is resistant to 0.04 U of bacitracin.
True
9
What is the original colour of VP test?
Colorless
10
What are the specimens used in the Laboratory Diagnosis of Staphylococci?
Aspirated secretion (best sample), Purulent exudates, Joint fluids
11
Also known as lemon yellow colonies
Staphylococcus citreus
12
True/False: Micrococcus is not susceptible to 0.04 U of bacitracin
False
13
It coagulates the fibrinogen in the plasma. Promotes the formation of a fibrin layer around the staphylococci abscess thereby protecting the bacteria from phagocytosis.
Coagulase
14
It is bound to the cell wall and clots human, rabbit, or pig plasma by directly converting fibrinogen into fibrin.
Cell-bound coagulase or clumping factor
15
They are Catalase-producing and facultatively anaerobic except for S. saccharolyticus.
Staphylococci
16
How can bacteria get to the bone?
Via bloodstream, Following an injury
17
The exception in staphylococci because it is an obligate anaerobe
Staphylococcus saccharolyticus
18
What is the positive result for Tube method?
Clot or coagulation formation after one to four hours of incubation
19
What are the two method that can be used in Coagulase test?
Slide method, Tube method
20
True/False: Staphylococcus is fermentative
True
21
It is considered sensitive but definitive method, detects extracellular or free coagulase.
Tube method
22
It is the other important of the cell wall aside the peptidoglycan layer.
Teichoic acid
23
What are the different types of Hemolysin?
Alpha-hemolysin, Beta-hemolysin, Delta-hemolysin, Gamma-hemolysin
24
This family is consist of Gram positive cocci, arranged in tetrads, clusters
Micrococcaceae
25
Where does S. aureus grow?
Ordinary media
26
True/False: Staphylococcus is microdase positive.
False
27
Also known as golden yellow colonies
Staphylococcus aureus
28
He was the first to see staphylococci in pus specimen in 1878.
Robert Koch
29
Staphylcocci have ___ species and ___ sub species.
32, 15
30
It is an extensive exfoliative dermatitis that occurs primarily in newborns and previously healthy children.
Scalded skin syndrome
31
It is a very superficial skin infection common in children, usually produces blisters or sores on the face, neck, hands and diaper area. It is characterized by watery bristles, which become pustules and then honey colouted crust.
Impetigo
32
It breaks down penicillin and other B-lactam drugs. More than 90% of clinical staphylococci isolates are penicillin-resistant as a result of enzyme production.
B-lactamase
33
Staphylococci are gram positive cocci which is classified into these 2 families:
Micrococcaceae, Streptococcaceae
34
It is the major structural component of the cell wall. It is important in the pathogenesis of staphylococcal infections.
The peptidoglycan layer
35
What are the 4 genera of Micrococcaceae?
Planococcus, Micrococcus, Stomatococcus, Staphylococcus
36
What does Staphylococcus aureus and S. lugdunensis have in common which makes it possible to confuse it with each other?
mecA gene
37
Greek word 'Kokkus' means
berry
38
Type of Coagulase
Cell-bound coagulase or clumping factor, Unbound or free coagulase
39
Who first cultured in liquid media?
Louis Pasteur
40
Who first observed in pus in 1871?
Friedrich von Recklinghausen
41
He named S. aureus and S. albus in 1884
Rosenbach
42
What does CONS stands for?
Coagulase negative staphylococcus
43
What is the positive result for Mannitol fermentation test?
Yellow coloured S.aureus colonies
44
True/False: Staphylococcus is not susceptible to antibiotic furazolidone.
False
45
It is used to screen catalase positive colonies, detects cell bound coagulase or clumping factor.
Slide method
46
It attacks and kills white blood cells, pore forming exotoxin that suppresses phagocytosis and responsible for necrotizing skin and soft tissue infections.
Leukocidin/Panton-Valentine leukocidin (Cytolytic toxin)
47
He named the bacteria as "staphylococcus" in 1881
Sir Alexander Ongston
48
It is an extracellular enzyme that is not bound to the cell wall and causes clot formation when bacterial cells are incubated with plasma.
Unbound or free coagulase
49
It is an aggregation of infected furuncles. it may form large abscesses. It is a large are of redness, swelling and pain, punctuated by several sites of drainage pus.
Carbuncle
50
Other slide coagulase Staphylococcus are:
S. lugdunensis, S. schleiferi
51
Most commonly used method of Coagulase test
Tube method
52
Species of Staphylococcus that are causes of endocarditis
Staphylococcus lugdunensis, Staphylococcus aureus
53
A method of Coagulase test that is not used nowadays
Slide method
54
What are the differential tests for Staphylococcus aureus?
Coagulase test, Mannitol fermentation test, Tellurite glycine agar, Polymyxin sensitivity test, Voges-Proskauer (VP) test, Deoxyribonuclease (Dnase) test
55
Specie of Staphylococcus that is a cause of urinary tract infection
Staphylococcus saprophyticus
56
It refers to the inflammation of the bone
Osteomyelitis
57
It is a folliculitis affecting one or more hair follicles on the edge of the upper or lower eyelid.
Sty
58
It is less toxic than a and B-lysins, produced by all S.aureus strains that cause RBC injury in culture and produces edematous lesions.
Gamma-hemolysin
59
It destroys RBC and is associated with the Panton-Valentine leukocidin.
Delta-hemolycin
60
Staphylocci which are Coagulase negative
S. epidermidis, S. hemolyticus, S. saprophyticus, S. hominis, S. capitis, S. schleiferi, S. simulans, S. warneri
61
It causes honeymoon cystitis
Staphylococcus saprophyticus
62
What is the pH indicator for Mannitol fermentation test?
Phenol red
63
A unique property of _________ is its ability to bind to the Fc part of all IgG molecules except IgG3.
Protein A
64
It is a chromosomal-mediated toxin and causes almost all cases of menstruation-associated TSS and it stimulates the production of a large amount of cytokines that are responsible for the symptoms.
Toxic shock syndrome toxin 1 (TSST-1)/ Enterotoxin F/Pyogenic exotoxin
65
True/False: Micrococcus is resistant to antibiotic furazolidone.
True
66
True/False: Micrococcus utilize sugars oxidatively or not at all in the O/F test.
True
67
It enhances invasion and survival in the tissue. Breaks down the hyaluronic acid that is present in the intracellular ground substances of connective tissues, resulting in the spread of bacteria.
Hyaluronidase (Spreading-factor enzyme)
68
Species of Staphylococcus which are causes for nosocomial infection
Staphylococcus epidermidis, Staphylococcus hemolyticus
69
What is the Family of Staphylococcus?
Micrococcaceae
70
True/False: Micrococcus is lysed with lysostaphin (sensitive).
False
71
It is a serin protease that divides the intracellular bridges of the epidermidis and causes extensive sloughing of the epidermis to produce a burn-like effect on the patient.
Exfoliatin serotypes A and B (Superantigens)/ Epidermolytic toxins A and B