Neisseria Part 2

56問 • 2年前

問題一覧

•Colonies on CAP is similar to the T3 colonies of N. gonorrhoea •Colistin susceptibility test differentiates N. cinerea from N. gonorrhoea •Grows on MH but not on TMA

Neisseria cinerea

It differentiates N. cinerea from N. gonorrhoea

Colistin susceptibility test

•Asaccharolytic, yellow pigmented Neisseria species •Ability to grow on BAP and CAP at 22 degree centigrade

Neisseria flavescens

•Usually isolated from nasopharynx of children or young adults •Grow in NA incubated at 22 degree centigrade •Colonies appear large and very mucoid

Neisseria mucosa

It is isolated from meningococcal carrier surveys, commonly in children who are two years of age and rarely from adults

Neisseria lactamica

• It will grow in NA incubated at 25 degree centigrade • Colonies appear like bread crumbs

Neisseria sicca

•it is a "rod-shaped" gram-negative coccus •weakly positive or negative catalase test

Neisseria elongata

•it is a " rod-shaped" gram-negative coccus • Positive catalase test but does not produce acid from any of the carbohydrates that are used to identify Neisseria species.

Neisseria weaveri

• Most commonly isolated member of the genus Moraxella • Part of the microbiota of the oropharynx and is an opportunistic pathogen • Resembles Neisseria species by exhibiting Gram-negative coccal morphology • Non-motile, fastidious, ß-lactamase producer and is encapsulated with common pili

Moraxella catarrhalis (Branhamella catarrhalis)

It causes URT infections in otherwise healthy children and the elderly. And is the third most common cause of otitis media and sinusitis in children.

Moraxella catarrhalis (Branhamella catarrhalis)

Also known as Branhamella catarrhalis

Moraxella catarrhalis

How does Moraxella catarrhalis look like under the microscope?

Small, gram-negative cocci that tends to grow in pairs and have a hockey puck" appearance

What are the Laboratory Diagnosis method used for Moraxella catarrhalis?

Gram stain, Culture, Catalase and oxidase test, CHO utilizatiom test, Dnase test, Butyrase esterase/Tributyrin test

It is the definitive test for M. catarrhalis

Butyrase esterase/Tributyrin test

What is substrate used in Butyrate esterase/Tributyrin test?

Bromo-chloro-indolyl butyrate

What is the positive result for Butyrate esterase/Tributyrin test?

Blue color

What are the media used in the laboratory diagnosis of Moraxella catarrhalis?

BAP and CAP

How does Moraxella catarrhalis looks like under the microscope?

Prescence of Intracellular diplocci

True/False: Moraxella catarrhalis is catalase and oxidase postive.

True

Result of Moraxella catarrhalis in CHO utilizatiom test

Negative

•definitive test for M. catarrhalis •(+) result Exhibits a clear or colorless halo around the colonies after 24 hours incubation.

Dnase test

What are the different Non-pathogenic Neisseria species?

Neisseria cinerea, Neisseria flavescens, Neisseria mucosa, Neisseria lactamica, Neisseria sicca, Neisseria elongata, Neisseria weaveri

It accelerates antibody and antigen through a buffered diffusion medium

Counterimmunoelectrophoresis

It is the direct identification of antigens in CSF, serum, and urine

Latex agglutination

What medium is used in the Antimicrobial test of Neisseria meningitidis?

Laked horse blood or MHA with 5% sheep blood

What method is used in the Antimicrobial test of Neisseria meningitidis?

Broth microdilution or agar dilution MIC test with cation-supplemented MH broth

It is the primary treatment in the Antimicrobial test of Neisseria meningitidis

Penicillin G

What is the positive result of Neisseria meningitidis in Oxidase test?

Purple color

N. meningitidis is sensitive to SPS, so the content in blood culture broth should not exceed to __________.

0.025%

In the culture of Neisseria meningitidis, where should Nasopharyngal swabs be placed?

JEMBEC system

What are the culture media used in Laboratory diagnosis of Neisseria meningitidis?

BAP, CAP, TMA

What does Neisseria mengitidis looks like in Gram stain?

Reveals Gram-negative intracellular and extracellular diplococci

Neisseria meningitidis produces this enzyme

Gamma-glutamyl aminopeptidase

What are laboratory diagnosis method used for Neisseria meningitidis?

Gram stain, Culture media, Oxidase test, Antimicrobial test, Immunologic test, Gamma-glutamyl aminopeptidase test

What are the specimens used for the Laboratory diagnosis of Neisseria meningtidis?

CSF, Blood, Nasopharyngeal swabs, Petechial skin lesions

What are the sourde of epidemics of Meningococcemia?

Oral secretions, Respiratory droplets

What are the signs and symptoms of Meningococcemia?

Frontal headache, Stiff neck, Fever

What is drug of choice for the treatment of Meningococcemia?

Penicillin

• It refers to the presence of N. meningitidis in the blood, occur in acute or chronic form • Occurs with or without meningitis • Source of epidemics are oral secretions and respiratory droplets •Signs and symptoms: frontal headache, stiff neck, and fever. • Petechial skin lesions may develop during bacteremic spread due to the release of endotoxin after a bacterial cell lysis • Penicillin is the drug of choice

Meningococcemia

What is the related infection of Neisseria meningitidis?

Meningococcemia

True/False: Neisseria meningitidis is Beta-lactamase negative

False

What are the virulence factor of Neisseria meningitidis?

Pili, Polysaccharife capsules, Cellular membranes(pro A and B)

What are the Serogroups of Neisseria meningitidis?

A, B, C, Y and W-135

What does Neisseria meningitidis looks like in culture media?

Encapsulated strains are mucoid in appearance. Colonies appear bluish gray on BAP; Small, tan, and mucoid on CAP.

What does Neisseria meningitidis looks like under the microscope?

Encapsulated strains, halo around the organism

Also known as Meningococci

Neisseria meningitidis

• Causative agent of meningococcemia or spotted fever • Leading cause of fatal bacterial meningitis • Found in nasopharynx and oropharynx • Commensal of the URT • Glucose and maltose fermenter, requires iron for growth

Neisseria meningitidis ( Meningococci)

What is the Preferred method for Antimicrobial susceptibility test?

Disk diffusion or agar dilution

What is the Preferred medium for Antimicrobial susceptibility test?

GC agar

It may be used as an alternative method instead of Antimicrobial susceptibility test

E-test

What are the Antibiotics that are utilized in the Antimicrobial susceptibility test (AST) of Neisseria gonorrhoea?

Extended-spectrum Cephalosporins and Quinolones

What are the specimens used for Chemiluminiscent nucleic acid probe?

Endocervical or urethral swabs

• It is the rapid test for directly detecting gonococcal rRNA in genital and conjunctival specimens and which provides results within hours. • Not recommended for pharyngeal or rectal specimens

Chemiluminescent nucleic acid probe

What are the specimens used in Nucleic acid amplification test?

Endocervical and urethral swabs and urine

•It detects gonococcal antigen or nucleic acid directly in specimens •Has the capability to detect Chlamydia trachomatis.

Nucleic acid amplification test

What are the different tests under Molecular assays?

Nucleic acid amplification test, Chemiluminescent nucleic acid probe

Translation(Pre-finals)

MAHATHIR ALPHA · 55問 · 2年前

Translation(Pre-finals)