問題一覧
1
It is the orderly production of mature granulocytes (N,E,B)
Granulocyte production
2
How long is the maturation sequence of Granulocytes?
About 14 days
3
What is the Normal range of WBC at birth or infants?
10,000-25,000/uL of blood
4
What is the Normal range of WBC of Infants upto 1 year of age?
6000-16000/uL of blood
5
What is the Normal range of WBC of Adults?
4000-11000/uL of blood
6
Try to Memorize!
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7
It refer to the detection of changes in numbers of circulating WBCs(percentage of each type). It indicates infection, poisoning, leukemia, chemotheraphy, parasites or allergy reactions.
Differential WBC counts
8
In this stage of the granulocyte, The size of the cell is 15-20 um, has a round/slightly oval nucleus, a fine chromatin and 2-5 Nucleoli.
Myeloblast
9
In this stage of the granulocyte, the size of the cell is 15-21 um, has a oval/round nucleus, a slightly coarse chromatin and 2-3 Nucleoli.
Promyelocyte
10
In this stage of the granulocyte, the size of the cell is 12-18 um, has a oval/round nucleus, a coarser and condensed chromatin and no Nucleoli.
Myelocyte
11
In this stage of the granulocyte, the size of the cell is 10-16 um, has a Unsegmented/bi lobed nucleus, a condensed nucleus and no Nucleoli.
Basophil
12
What is the cytoplasm staining of Promyelocyte?
Basophilic
13
What is the cytoplasm staining of myelocyte?
Basophilic acidophilic
14
What is the cytoplasm staining of Metamyelocyte?
Beige/Salmon
15
What is the cytoplasm staining of Band?
Beige/Salmond
16
What is the cytoplasm staining of Neutrophil, eosinophil and basophil?
Beige/Salmond
17
1st recognizable in granulocytic series
Myeloblast
18
First appearance of primary granules
Promyelocyte
19
First appearance of secondary granules
Myelocyte
20
Youngest stage
Metamyelocyte/juvenile
21
Youngest cell to appear in peripheral blood
Band
22
N, E, B
Mature granulocyte
23
What are the Secondary or Specific Granules found in Neutrophil?
Aminopeptidase, Collagenase, Muramidase, Lactoferrin, Lysozyme, Basic proteins, Plasminogen activator, Alkaline phosphatase
24
What are the Larger Secondary or Specific granules/Major basic Proteins found in Eosinophil?
Acid hydrolase, Peroxidase, Phospholipase, Cathepsin, Eosinophilic Cationic CHON, Eosinophil-derived neurotoxin
25
What are the Smaller Secondary or Specific Granules found in Eosinophil?
Arylsulfatase, Peroxidase, Acid phosphatase
26
What are the Secondary or Specific Granules found in Basophil?
Histamine, Heparin, Peroxidase, Eosinophilic Chemotactic Factor A
27
What is the other term for Neutrophil?
Polymorphonuclear (PMN)
28
What are the granules found in Neutrophil?
Pink-rose violet granules
29
What is the Ratio of primary to secondary granules of Mature neutrophil?
2-3:1
30
In Neutrophil, it competes with bacteria for Fe (possibly inhibiting growth) and may promote PMN adherence to endothelial cells.
Lactoferrin-Fe binding glycoprotein
31
What is the life span of Neutrophil/Polymorphonuclear (PMN)?
9-10 days
32
What are the POOLS of Neutrophils in the body?
Bone marrow-production and maturation, Blood
33
What are the POOLS of Neutrophils in the Blood?
Circulating pool, Marginating pool
34
What are the POOLS of Neutrophil in the Bone marrow production and maturation?
Mitotic pool, Maturing pool, Storage pool
35
What are found in the Mitotic pool?
Myeloblast, Promyelocyte, Myelocyte
36
What are found in the maturing pool?
Metamyelocyte
37
What are the Secondary or Specific Granules found in the Storage pool?
Metamyelocyte, Bands, Segmented PMN's
38
What are found in the Circulating pool?
50% of PMNs circulating freely
39
What is found in the Marginating pool?
50% PMNs that adhere to the vessel walls or are sequenstered in the capillaries
40
What are the granules found on Eosinophil?
Reddish-orange with affinity for eosin(acidic)
41
It responds to parasitic and helminthic infection and allergy.
Eosinophil
42
True/False: Concentration of Eosinophil is normally high at night or in the afternoon.
True
43
It is the Major basic CHON -arginine- rich protein that plays a major role in killing parasites
Eosinophil
44
Its major function is to provide defense against helmenthic parasites and it has a role in allergic reactions by lessening hypersensitivity reactions through the release of an amine oxidase, which neutralizes histamine.
Eosinophil
45
What does Eosinophil releases that neutralize histamine?
Amine oxidase
46
It is formed from the disintegration of eosinophils and is made up of lysophospholipase found in the cytoplasm of eosinophils.
Charcot-Leyden Crystal
47
What is Charcot-Leyden crystal made of?
Lysophospholipase
48
Charcot-Leyden crystal are seen in what conditions?
Allergic asthma (nasal mucos), Pulmonary eosinophilic infiltrates (pleural fluid), Parasitic infection (stool)
49
It responds to allergic or hypersensitivity reactions a such as allergic asthma and delayed-onset allergy reaction
Basophil
50
What are the granules found in Basophil?
Water soluble blue black specific granules with affinity to methylene blue
51
What makes up 60-80 percent of the lymphoid population?
T lymphocyte
52
What makes up 10-20 percent of the lymphoid population?
B lymphocytes
53
What makes up 10 percent percent of the lymphoid population?
Null lymphocyte
54
Long lived lymphocyte (4-10 years) with cellular-mediated immunity
T lymphocyte
55
Short-lived lymphocyte with humoral-mediated immunity
B lymphocyte
56
Lymphocyte with tumor host defense
Null lymphocytes
57
What are the stages of lymphocyte?
Lymphoblast, Prolymphocyte, Mature small lymphocyte, Medium lymphocyte, Large lymphocyte
58
What are the stages of Plasma cells?
Plasmablast, Proplasmacyte, Plasmacyte/plasma cell
59
In this stage of the lymphocyte, the size of the cell is 10-18 um, it has a coarse chromatin, round/oval nucleus, a coarse 1-2 Nucleoli.
Lymphoblast
60
In this stage of the lymphocyte, the size of the cell is the same as lymphoblast or smaller, it has a more clumped chromatin, round/oval nucleus, a coarse 1-2 Nucleoli.
Prolymphocyte
61
In this stage of the lymphocyte, the size of the cell is 8-10 um, it has a dense chromatin, round/oval nucleus, a not visible Nucleoli.
Mature small lymphocyte
62
In this stage of the lymphocyte, the size of the cell is 10-12 um, chromatin not as dense as small lymphocyte.
Medium lymphocyte
63
In this stage of the lymphocyte, the size of the cell is 12-16 um, it has a round/oval nucleus and nucleoli is not visible, abundant clear and very pale blue cytoplasm
Large lymphocyte
64
It is a stage of lymphocyte wherein its cytoplasm appears smooth, has no granules and moderate dark blue in color
Lymphoblast
65
It is a stage of lymphocyte wherein its cytoplasm is nongranular, moderate and darkblue in color
Prolymphocyte
66
It is a stage of lymphocyte wherein its cytoplasm is thin rim around nucleus and Robin's egg blue.
Mature small lymphocyte
67
It is a stage of lymphocyte wherein its cytoplasm is more abundant, pale moderate blue in color.
Medium Lymphocyte