Mycobacteria Part 2

100問 • 2年前

問題一覧

True/False: Mycobacterium kansasii is contagious from person to person

False

There was a princess named Sheka who was interested in viewing Cultures, in one of her endeavors while observing one of the culture that was offered to her. She noticed smooth to rough colonies with dark centers and waxy edges-M8 7H10agar photochromogenic colonies-"dark red crystals of 10-8-carotene". What do you think is the bacteria the princess has viewed?

Mycobacterium kansasii

What is the biochemical tests and results of Mycobacterium kansasii?

(+) Tween 80 hydrolysis in 3 days and pyrazinamidase Strong nitrate reduction

It causes diseases of the fish and has been isolated from an aquarium.

Mycobacterium marinum

It is the causative agent of "swimming pool granuloma".

Mycobacterium marinum

It is usually indicated by a red or blue-red subcutaneous nodule on the elbow, knee, toe or finger a cutaneous Infection in human which occur when traumatized skin came in contact with inadequately chlorinated freshwater or saltwater.

Swimming pool granuloma

Sheka was observing the microscope and she noticed moderate to long rods with cross-barring, what do you think is the bacteria present under the microscope?

Mycobacterium marinum

Sheka was observing a culture and she noticed smooth to rough and wrinkled deep blue colonies (photochromogenic), what do you think is the bacteria present in the media?

Mycobacterium maninum

What is the preferred growth temperature for Mycobacterium marinum?

28-32°C (preferred low temperature)

What is the natural reservoir for Mycobacterium marinum

Fresh water Salt water

What are the biochemical test and result for Mycobacterium marinum?

(+) Tween 80 hydrolysis (+) Urease (+) Pyrazinamidase

It is the 3rd most common Mycobacterium species after M. tuberculosis and M. leprae.

Mycobacterium ulcerans

It is a rare cause of Buruli ulcer, a painless nodule under the skin after previous trauma.

Mycobacterium ulcerans

A painless nodule under the skin after previous trauma rarely caused by Mycobacterium ulcerans

Buruli ulcer

Sheka was observing the microscope and she noticed moderately long rods without beading, what do you think is the bacteria present under the microscope?

Mycobacterium ulcerans

Sheka was observing a culture and she noticed smooth and rough nonpigmented colonies (6-12 weeks incubation), what do you think is the bacteria present in the media?

Mycobacterium ulcerans

How long is the incubation of Mycobacterium ulcerans in culture?

6-12 weeks

What are the biochemical test and results for Mycobacterium ulcerans?

(+) heat-stable catalase

What is the Preferred growth temperature for Mycobacterium ulcerans?

30-33°C

What is the other term for Mycobacterium gordonae?

Tap water bacillus

This bacteria is caused by a problem in the preparation of bacteriologic smears.

Mycobacterium gordonae

True/False: Mycobacterium gordonae rarely causes infection in humans

True

Sheka was observing a culture and she noticed smooth, yellow-orange colonies (scotochromogen), what do you think is the bacteria present in the media?

Mycobacterium gordonae

What are the biochemical test and results for Mycobacterium gordonae?

(+) Tween 80 hydrolysis (+) Heat-stable catalase (-) Nitrate reduction

What is the Preferred growth temperature for Mycobacterium gordonae?

22 to 37°C

This bacteria can be recovered from hot and cold water taps, especially water storage tanks of hospitals, and from birds.

Mycobacterium xenopi

This bacteria is first isolated from an african toad.

Mycobacterium xenopi

It causes pulmonary infection in adults; a potential pathogen.

Mycobacterium xenopi

Sheka was observing a culture and she noticed small colonies with dense centers and filamentous edges, what do you think is the bacteria present in the media?

Mycobacterium xenopi

Describe the appearance of Mycobacterium xenopi in MB 7H10 agar

Round colonies with branching filaments

Appearance of Mycobacterium xenopi in cornmeal-glycerol agar

"bird's nest" appearance with sticklike projections (young colonies)

It can be either nonphotochromogen or scotochromogen (bright yellow colonies).

Mycobacterium xenopi

What is preferred growth temperature for Mycobacterium xenopi?

42 degree celsius

What is the biochemical test and result for Mycobacterium xenopi?

(+) heat stable Catalase (+) Pyrazinamidase (+) Arysulfatase

Mycobacterium terrae is also known as what?

Radish bacillus

It normally saprophytic and rarely causes human infections.

Mycobacterium terrae complex

What are the species included in Mycobacterium terrae complex?

Mycobacterium terrae Mycobacterium triviale Mycobacterium nonchromogen

Sheka was observing the microscope and she noticed long and filamentous rods, what do you think is the bacteria present under the microscope?

Mycobacterium xenopi

Sheka was observing the microscope and she noticed short to medium coccobacilli, what do you think is the bacteria present under the microscope?

Mycobacterium terrae complex

This specie in Mycobacterium terrae complex has rough and dry colonies

Mycobacterium triviale

This specie in Mycobacterium terrae complex has smooth colonies

Mycobacterium terrae

This specie in Mycobacterium terrae complex has smooth to rough and white to buff colonies

Mycobacterium nonchromogenicum

What are the biochemical test and result for Mycobacterium terrae-complex?

(+) Tween 80 hydrolysis (+) Heat-stable catalase reduction of nitrate

This specie of Mycobacterium terrae-complex has positive growth in 5% NaCl

Mycobacterium triviale

What infection/s does Mycobacterium kansasii cause?

Chronic pulmonary disease Extrapulmonary diseases such as cervical lymphadenitis and cutaneous disease

What infection/s does Mycobacterium marinum cause?

Cutaneous disease Bacteremia

What infection/s does Mycobacterium scrofulaceum cause?

Cervical adenitis in children Bacteremia Pulmonary disease Skin infections

What infection/s does Mycobacterium gordonae cause?

Infections only in immunocompromised hosts

What are the natural reservoir of Mycobacterium gordonae?

Tap water Water Soil

What are the natural reservoir of Mycobacterium scrofulaceum?

Raw milk Soil Water Daily products

Infection is more common in white males; natural reservoir is tap water; aerosols are involved in transmission.

Mycobacterium kansasii

It is usually associated with aquatic activity usually involving fish

Mycobacterium marinum

How is Mycobacterium marinum transmitted?

By contact with contaminated water and organism entry by means of trauma or small breaks in the skin

What infection/s does Mycobacterium avium complex cause?

It causes pulmonary infections, cervical lymphadenitis and disseminated disease in people without aids; causes disseminated disease in people with aids

What infection/s does Mycobacterium xenopi cause?

Primarily pulmonary infections in adults Extrapulmonary infections Disseminated disease

What infection/s does Mycobacterium ulcerans cause?

Indolent cutaneous and subcutaneous infections

African Buruli ulcer or Australian Bairndale ulcer are caused by what bacteria?

Mycobacterium ulcerans

What infection/s does Mycobacterium haemophilum cause?

Disseminated disease Cutaneous infections in immunosuppressed adults Mild and limited skin infections Cervical lymphadenitis in children

What infection/s does Mycobacterium terrae complex cause?

Tenosynovitis Pulmonary disease

What is the natural reservoir of Mycobacterium avium complex?

Environmental sources including natural waters and soil

What is the natural reservoir of Mycobacterium xenopi?

Water especially hot water taps in hospitals; believed to be transmitted in aerosols

What is the natural reservoir of Mycobacterium ulcerans?

Stagnant tropical waters; also harbored in an aquatic insect's salivary glands

On what climate does infection caused by Mycobacterium occurs?

Tropical or temperate climate

What is the natural reservoir of Mycobacterium haemophilum?

Unknown

Where can Mycobacterium terrae-complex be found?

Aquatic environments

Contains saphrophytes; rarely cause human infection

Mycobacterium asiaticum

Mycobacterium celatum grow best at what temperature?

35 degree celsius

Fastidious mycobacteria; it does not grow on routine mycobacterial media; it has been recovered from BACTEC culture.

Mycobacterium genavense

This NTM requires hemoglobin or hemin for growth

Mycobacterium haemophilum

This NTM exhibit growth at 22 degree celsius and requires 7-12 weeks of incubation

Mycobacterium malmoense

What are biochemical test and result for Mycobacterium asiaticum?

(+) high level of heat-stable catalase

What are biochemical test and result for Mycobacterium genavense?

(+) semi-quantitative and heat-stable catalase (+) Pyrazinamidase (+) Urease

What are biochemical test and result for Mycobacterium Malmoense?

(+)Tween 80 hydrolysis (+) Heat-stable catalase (+) Pyrazinamidase

What are biochemical test and result for Mycobacterium scrofulaceum?

(+) high level heat-stable catalase (+)Urease

What are biochemical test and result for Mycobacterium simiaea?

(+) niacin (+) high level heat-stable catalase

Sheka was observing a culture and she noticed rough to smooth non pigmented colonies, what do you think is the NTM bacteria present in the media?

Mycobacterium haemophilum

Sheka was observing a culture and she noticed dysgonic colonies which requires 6-8 weeks of incubation, what do you think is the NTM bacteria present in the media?

Mycobacteriym genavense

Sheka was observing a culture and she noticed large non pigmented colonies, what do you think is the NTM bacteria present in the media?

Mycobacterium celatum

Sheka was observing a culture and she noticed dysgonic, smooth and pigmented colonies, what do you think is the NTM bacteria present in the media?

Mycobacterium asiaticum

Sheka was observing a culture and she noticed nonpigmented smooth, glistening and opaque colonies with dense center, what do you think is the NTM bacteria present in the media?

Mycobacterium malmoense

Sheka was observing a culture and she noticed light yellow to deep orange smooth colonies with dense centers (scotochromogen), what do you think is the NTM bacteria present in the media?

Mycobacterium scrofulaceum

Sheka was observing a culture and she noticed Yellow smooth colonies (photochromogen), what do you think is the NTM bacteria present in the media?

Mycobacterium simiae

Sheka was observing a culture and she noticed Yellow to orange smooth and rough colonies (scotochromogen), what do you think is the bacteria present in the media?

Mycobacterium szulgai

What are the media used in the culture of Mycobacterium haemophilum?

CAP MHA with 5% Fildes enrichment LJ with 2% ferric ammonium citrate

Sheka was observing the microscope and she noticed acid-fast coccoid cells, what do you think is the NTM bacteria present under the microscope?

Mycobacterium asiaticum

Sheka was observing the microscope and she noticed strongly acid-fast; short or curved, without beading, in clusters or cords, what do you think is the NTM bacteria present under the microscope?

Mycobacterium haemophilum

Sheka was observing the microscope and she noticed short coccobacilli without cross-bands, what do you think is the NTM bacteria present under the microscope?

Mycobacterium malmoense

Sheka was observing the microscope and she sees medium to long rod, what do you think is the NTM bacteria present under the microscope?

Mycobacterium scrofulaceum

Sheka was observing the microscope and she noticed a short coccobacilli, what do you think is the NTM bacteria present under the microscope?

Mycobacterium simiae

Sheka was observing the microscope and she noticed medium to long rods with cross-barring, what do you think is the NTM bacteria present under the microscope?

Mycobacterium szulgai

They are considered potentially pathogenic and are found in soil, rivers, municipal water supplies, marine and terrestrial life forms.

NTM Rapid growers

Organisms gain entry into the host by inoculation into the skin and subcutaneous tissues during trauma, injections, surgery or animal contact.

NTM Rapid growers

Microscopy: weakly Gram-positive rods resembling diptheroids

NTM Rapid growers

Cultures: colonies appear on solid media in 7 days or less.

NTM Rapid growers

It is the most common rapidly growing mycobacteria associated with localized cutaneous infections - skin and soft tissue infections.

Mycobacterium fortuitum

Where is Mycobacterium fortuitum isolated from?

Water soil Dust

Microscopy: long and tapered to short, thick rods ( pleomorphic) have tendency to decolorize and appear partially AFB (old cultures)

Mycobacterium fortuitum

Culture: rough or smooth multilobate nonpigmented colonies - U medium branching

Mycobacterium fortuitum

What are the biochemical test and result of Mycobacterium fortuitum?

(+) 3-day Arylsulfatase tests Nitrate reduction

100

Describe the appearance of Mycobacterium fortuitum in MB 7H11

Filamentous colonies with aerial hyphae

Translation(Pre-finals)

MAHATHIR ALPHA · 55問 · 2年前

Translation(Pre-finals)