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問題一覧
1
It is known as the Foam-loving bacterium
Aggregalibacter aphrophilus
2
Haemophilus originates from the Greek word 'haima' and 'philus' which means what?
Blood lover
3
All Haemophilus are oxidase positive except for what specie?
Haemophilus segnis
4
Also known as Actinobacillus actinomycetemcomitans
Aggregalibacter actinomycetemcomitans
5
What is the biochemical test for Haemophilus influenzae?
Porphyrin (-)
6
It spreads from nasopharynx and the regional lymph nodes to the blood and finally to the Meninges.
Haemophilus influenzae
7
In the Gram stain of Brucella, ___________ should be substituted for safranin O to improve the Gram Stain
Carbol fuchsin
8
HACEK are fastidious and grow slowly on BAP & CAP & require _________ of incubation
7-14 days
9
It is the only member of the genus Haemophilus that produces IgA protease.
Haemophilus influenzae
10
What Haemophilus species contain/s v growth factor only?
Haemophilus parahaemolyticus Haemophilus parainfluenzae
11
Fastidious, non-motile, capnophilic, and facultatively anaerobic bacteria. And most species cannot grow on pure BAP
Haemophilus
12
What are the encapsulated strains of the Typeable form of Haemophilus influezae?
A, B, C, D, E and F
13
What should be done for recovery of Haemophilus ducreyi?
For recovery of H. ducreyi, the ulcer should be cleansed with a sterile gauze that is pre-moistened w/ sterile phosphate-buffered saline and plating is preferred instead of a transport medium for the isolation of H. ducreyi.
14
What are the Primary routes of Human infections caused by Brucella?
A. Ingestion of unpasteurized and contaminated milk or cheese from infected animals. B. Inhalation of air around animal carcasses (aerosol infection) C. Penetration of ocular or oral mucosa. D. Direct inoculation into the bloodstream through abrasions in the skin of needlestick injuries.
15
Sheka was observing a BAP and she sees colonies that are small, convex, translucent, yellowish and non-haemolytic. What bacteria do you think is present in the medium?
Brucella
16
This Haemophilus specie has tan and dry colonies; B- haemolytic
Haemophilus parahaemolyticus
17
It infects the aortic valve more freq. than the other HACEK species
Cardiobacterium hominis
18
What are the preferred specimen for the isolation of Brucella?
Blood and bone marrow
19
Describe the appearance of Haemophilus under the microscope
Gram-negative, small, pleomorphic coccobacilli or rods
20
What are the associated infection/disease with Haemophilus ducreyi?
Chancroid or soft chancre
21
It grow as “satellites” on BAP around bacterial colonies and produce NAD like S. aureus
V factor-dependent H. influenzae
22
True/False: Haemophilus need an immediate transport and processing for their isolation.
True
23
What are the associated infection/disease with Haemophilus parainfluenzae?
Endocarditis
24
This category of Haemophilus influenzae is based on the capsular characteristics of H. influenzae
Typeable form Haemophilus influenzae
25
This Haemophilus specie has Fructose and maltose fermentation
Haemophilus parainfluenzae
26
This Haemophilus specie is genetically related to Haemophilus influenzae
Haemophilus aegypticus
27
It shows "false Gram-positive" and the only indole-positive member of the HACEK group
Cardiobacterium hominis
28
Test for identifying the heme-producing species of Haemophilus
Porphyrin test
29
True/False: Haemophilus ducreyi is part of the indigenous human microbiota.
False
30
What is the selective media for Haemophilus ducreyi?
Selective Nairobi biplate medium (combination of gonococcal agar and MHA with horse’s blood and vancomycin)
31
Serotype can be determined through the identification of the distinct capsular antigen.
Serologic test
32
It is commonly found as indigenous microbiota of the URT of adult
Haemophilus parainfluenzae
33
It appears to have in "school-of-fish" gram stain
Haemophilus ducreyi
34
What are the associated infection/disease with Haemophilus influenzae biogroup aegyticus?
Brazilian purpuric fever
35
Haemophilus influenzae is very ___________ and can be rapidly killed by phagocytes.
Fastidious
36
What are the associated infection/disease with Haemophilus influenzae?
Meningitis Epiglottitis Arthritis
37
What is the other term for Haemophilus influenzae?
Pfeiffer's bacillus
38
What Haemophilus species contain/s x and v growth factors?
Haemophilus influenzae Haemophilus aegypticus Haemophilus influenzae biogroup aegypticus Haemophilus haemolyticus
39
What are the specimens used in the laboratory diagnosis of Haemophilus?
CSF, sputum, genital lesion or ulcer, joint fluid, vaginal swab, abscess drainage, conjunctival swab, bronchial washing & blood
40
It is the main cause of meningitis in children
Haemophilus influenzae
41
What are the growth factors of Haemophilus?
X factor (hemin) V factor (NAD)
42
What is the selective media for Haemophilus aegypticus?
CAP with 1% IsoVitaleX or Vitox
43
What is the other term for Brucella?
Bang's bacillus
44
H. aegypticus requires _____ days of incubation while H. ducreyi requires _____ days.
Four Seven
45
What are included in the HACEK(AACEK) group?
Aggregalibacter aphrophilus Aggregalibacter actinomycetemcomitans Cardiobacterium hominis Eikenella corrodens E. Kingella species
46
It is characterized by normal temperatures in the morning and then followed by high temperatures in the afternoon and evening.
Undulant fever
47
True/False: Turbidity of the specimen is an abnormal occurrence in the culture of Brucella
False
48
What are the tests used in the laboratory diagnosis of Haemophilus?
Gram stain Culture Porphyrin test Serological test
49
What are the test/s used in the Laboratory diagnosis of Brucella?
Gram stain Culture Serological test
50
What are the species of the genus Brucella?
Brucella abortus Brucella canis Brucella suis Brucella melitensis
51
Brucella are localized in tissues that are rich in__________________ & induces spontaneous abortion among animals.
Erythritol (placental tissue)
52
Obligately aerobic, fastidious, gram-negative coccobacilli and they replicate on ciliated respiratory epithelial cells of humans
Bordetella
53
What are the other related infections of Non-typeable form of Haemophilus influenzae?
Conjunctivitis Sinusitis (localized infections)
54
What is the mode of transmission of Haemophilus influenzae?
Person-to-person (droplets)
55
This Haemophilus specie is Non-typeable
Haemophilus influenzae biogroup aegypticus
56
No growth of Haemophilus occurs on what medium?
MacConkey agar
57
What is the other term for Haemophilus aegypticus?
Koch-Weeks bacillus
58
In the Culture of Brucella, isolates can be recovered after ______ but may require prolonged incubation up to ______.
7 days 30 days
59
Haemophilus species resemble an______________________ because of their pleomorphic appearances.
"amorphous serous material"
60
It is also known as the corroding bacilli
Eikenella corrodens
61
It is the preferred medium for Haemophilus because it contains the X and V factors.
CAP
62
What media are used in the Culture of Brucella?
BAP TSA Castaneda's medium
63
True/False: HACEK group does not causes slow & progressive bacterial endocarditis
False
64
What are the most virulent among the species of Brucella?
Brucella melitensis Brucella suis
65
It is the agent of chancroid which is highly communicable, sexually transmitted, genital ulcer disease.
Haemophilus ducreyi
66
It is the cause of serious infections in humans and leading cause of meningitis in unvaccinated children.
Haemophilus influenzae type b (Hib)
67
They are part of the indigenous microbiota & are considered as opportunistic pathogens. They are small, non-motile, cannot grown on MAC.
HACEK(AACEK) Group
68
Describe the appearance of brucella under the microscope
Small coccobacilli that are arranged singly, in pairs which have a “sandy appearance”
69
It detects the presence of enzymes that converts ALA into porphyrins.
Porphyrin test
70
What Serological test is used in Brucella?
Serum agglutination test
71
What are the media used for the culture of Haemophilus species?
CAP BAP BHI Thioglycollate
72
It is the second prevalent etiologic agent for otitis media with effusion (middle ear infection) after Streptococcus pneumoniae
Non-typeable form Haemophilus influenzae
73
It is used for rapid direct identification of the capsular antigens of H. influenzae.
Neufeld- Quellung reaction
74
It is the only catalase-positive HACEK specie
Aggregalibacter actinomycetemcomitans
75
What is preffered instead of a transport medium for the recovery of Haemophilus ducreyi?
Plating
76
What Haemophilus species contain/s x growth factor only?
Haemophilus ducreyi
77
Also known as the X factor
Hemin
78
This Haemophilus specie is B-haemolytic
Haemophilus haemolyticus
79
What are specimens that can be used in the Laboratory diagnosis of Brucella?
Blood Bone marrow Skin lesions Placental tissues
80
This category of Haemophilus influenzae does not produce capsules and part of the indigenous microbiota of the URT and adheres to human epithelial cells
Non-typeable form Haemophilus influenzae
81
They are non-motile, assacharolytic, and non-encapsulated. Some species require an increased supply of CO2 for growth.
Brucella
82
What are the associated infection/disease with Haemophilus aegypticus?
Pink eye conjunctivitis
83
When Haemophilus are grown anaerobically, only ____ & not hemin, is required.
NAD
84
They are obligate parasites on the mucous membranes of humans. And they die rapidly in clinical specimen and very susceptible to drying and extreme temperatures.
Haemophilus
85
What are the two categories of Haemophilus influenzae?
Typeable form Non-Typeable form
86
What is the principal Virulence factor of Haemophilus influenzae?
Polysaccharide capsule
87
What are the biochemical test and result for Haemophilus?
(+) catalase (+) oxidase except Haemophilus segnis
88
All species of Haemophilus normally inhabit the URT of humans except for what?
Haemophilus ducreyi
89
The distinguishing characteristic of this Haemophilus specie is School of fish
Haemophilus ducreyi
90
What are the human pathogens of the genus Haemophilus?
Haemophilus influenzae Haemophilus ducreyi Haemophilus parainfluenzae Haemophilus paraphrophilus Haemophilus parahaemolyticus Haemophilus pittmaniae Haemophilus aegypticus Haemophilus segnis
91
They are assacharolytic like the species of the genus Moraxella and the least common isolate of the HACEK group
Eikenella corrodens
92
This Haemophilus specie has mouse/bleach-like odor; non-haemolytic
Haemophilus influenzae
93
Also known as the V factor
NAD
94
What are the hallmarks of chanroid caused by Haemophilus ducreyi?
Buboes or suppurative, enlarged, draining, inguinal lymph nodes.
95
Describe the appearance of Haemophilus ducreyi in CAP
Colonies are transparent, small, non-mucoid, and tan or yellow.
96
What are the other Virulence factors of Haemophilus influenzae aside the polysaccharide capsule?
IgA protease Fimbriae Lipopolysaccharide
97
It is formerly known as Haemophilus aphrophilus
Aggregalibacter aphrophilus
98
What is the selective media for Haemophilus influenzae?
Horse’s blood-bacitracin agar for respiratory secretions of patients with cystic fibrosis.
99
Species of this genus are important human and animal pathogens. Obligate aerobes and intracellular parasites.
Brucella
100
Haemophilus species grow best at ______ degree cent., except H. ducreyi which grows at ___ degree cent. & in an environment with ___________.
35-37 35 5% to 10% CO2