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Small, Pleomorphic Gram-negative bacilli Part 1
  • MAHATHIR ALPHA

  • 問題数 100 • 12/24/2023

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    問題一覧

  • 1

    It is known as the Foam-loving bacterium

    Aggregalibacter aphrophilus

  • 2

    Haemophilus originates from the Greek word 'haima' and 'philus' which means what?

    Blood lover

  • 3

    All Haemophilus are oxidase positive except for what specie?

    Haemophilus segnis

  • 4

    Also known as Actinobacillus actinomycetemcomitans

    Aggregalibacter actinomycetemcomitans

  • 5

    What is the biochemical test for Haemophilus influenzae?

    Porphyrin (-)

  • 6

    It spreads from nasopharynx and the regional lymph nodes to the blood and finally to the Meninges.

    Haemophilus influenzae

  • 7

    In the Gram stain of Brucella, ___________ should be substituted for safranin O to improve the Gram Stain

    Carbol fuchsin

  • 8

    HACEK are fastidious and grow slowly on BAP & CAP & require _________ of incubation

    7-14 days

  • 9

    It is the only member of the genus Haemophilus that produces IgA protease.

    Haemophilus influenzae

  • 10

    What Haemophilus species contain/s v growth factor only?

    Haemophilus parahaemolyticus Haemophilus parainfluenzae

  • 11

    Fastidious, non-motile, capnophilic, and facultatively anaerobic bacteria. And most species cannot grow on pure BAP

    Haemophilus

  • 12

    What are the encapsulated strains of the Typeable form of Haemophilus influezae?

    A, B, C, D, E and F

  • 13

    What should be done for recovery of Haemophilus ducreyi?

    For recovery of H. ducreyi, the ulcer should be cleansed with a sterile gauze that is pre-moistened w/ sterile phosphate-buffered saline and plating is preferred instead of a transport medium for the isolation of H. ducreyi.

  • 14

    What are the Primary routes of Human infections caused by Brucella?

    A. Ingestion of unpasteurized and contaminated milk or cheese from infected animals. B. Inhalation of air around animal carcasses (aerosol infection) C. Penetration of ocular or oral mucosa. D. Direct inoculation into the bloodstream through abrasions in the skin of needlestick injuries.

  • 15

    Sheka was observing a BAP and she sees colonies that are small, convex, translucent, yellowish and non-haemolytic. What bacteria do you think is present in the medium?

    Brucella

  • 16

    This Haemophilus specie has tan and dry colonies; B- haemolytic

    Haemophilus parahaemolyticus

  • 17

    It infects the aortic valve more freq. than the other HACEK species

    Cardiobacterium hominis

  • 18

    What are the preferred specimen for the isolation of Brucella?

    Blood and bone marrow

  • 19

    Describe the appearance of Haemophilus under the microscope

    Gram-negative, small, pleomorphic coccobacilli or rods

  • 20

    What are the associated infection/disease with Haemophilus ducreyi?

    Chancroid or soft chancre

  • 21

    It grow as “satellites” on BAP around bacterial colonies and produce NAD like S. aureus

    V factor-dependent H. influenzae

  • 22

    True/False: Haemophilus need an immediate transport and processing for their isolation.

    True

  • 23

    What are the associated infection/disease with Haemophilus parainfluenzae?

    Endocarditis

  • 24

    This category of Haemophilus influenzae is based on the capsular characteristics of H. influenzae

    Typeable form Haemophilus influenzae

  • 25

    This Haemophilus specie has Fructose and maltose fermentation

    Haemophilus parainfluenzae

  • 26

    This Haemophilus specie is genetically related to Haemophilus influenzae

    Haemophilus aegypticus

  • 27

    It shows "false Gram-positive" and the only indole-positive member of the HACEK group

    Cardiobacterium hominis

  • 28

    Test for identifying the heme-producing species of Haemophilus

    Porphyrin test

  • 29

    True/False: Haemophilus ducreyi is part of the indigenous human microbiota.

    False

  • 30

    What is the selective media for Haemophilus ducreyi?

    Selective Nairobi biplate medium (combination of gonococcal agar and MHA with horse’s blood and vancomycin)

  • 31

    Serotype can be determined through the identification of the distinct capsular antigen.

    Serologic test

  • 32

    It is commonly found as indigenous microbiota of the URT of adult

    Haemophilus parainfluenzae

  • 33

    It appears to have in "school-of-fish" gram stain

    Haemophilus ducreyi

  • 34

    What are the associated infection/disease with Haemophilus influenzae biogroup aegyticus?

    Brazilian purpuric fever

  • 35

    Haemophilus influenzae is very ___________ and can be rapidly killed by phagocytes.

    Fastidious

  • 36

    What are the associated infection/disease with Haemophilus influenzae?

    Meningitis Epiglottitis Arthritis

  • 37

    What is the other term for Haemophilus influenzae?

    Pfeiffer's bacillus

  • 38

    What Haemophilus species contain/s x and v growth factors?

    Haemophilus influenzae Haemophilus aegypticus Haemophilus influenzae biogroup aegypticus Haemophilus haemolyticus

  • 39

    What are the specimens used in the laboratory diagnosis of Haemophilus?

    CSF, sputum, genital lesion or ulcer, joint fluid, vaginal swab, abscess drainage, conjunctival swab, bronchial washing & blood

  • 40

    It is the main cause of meningitis in children

    Haemophilus influenzae

  • 41

    What are the growth factors of Haemophilus?

    X factor (hemin) V factor (NAD)

  • 42

    What is the selective media for Haemophilus aegypticus?

    CAP with 1% IsoVitaleX or Vitox

  • 43

    What is the other term for Brucella?

    Bang's bacillus

  • 44

    H. aegypticus requires _____ days of incubation while H. ducreyi requires _____ days.

    Four Seven

  • 45

    What are included in the HACEK(AACEK) group?

    Aggregalibacter aphrophilus Aggregalibacter actinomycetemcomitans Cardiobacterium hominis Eikenella corrodens E. Kingella species

  • 46

    It is characterized by normal temperatures in the morning and then followed by high temperatures in the afternoon and evening.

    Undulant fever

  • 47

    True/False: Turbidity of the specimen is an abnormal occurrence in the culture of Brucella

    False

  • 48

    What are the tests used in the laboratory diagnosis of Haemophilus?

    Gram stain Culture Porphyrin test Serological test

  • 49

    What are the test/s used in the Laboratory diagnosis of Brucella?

    Gram stain Culture Serological test

  • 50

    What are the species of the genus Brucella?

    Brucella abortus Brucella canis Brucella suis Brucella melitensis

  • 51

    Brucella are localized in tissues that are rich in__________________ & induces spontaneous abortion among animals.

    Erythritol (placental tissue)

  • 52

    Obligately aerobic, fastidious, gram-negative coccobacilli and they replicate on ciliated respiratory epithelial cells of humans

    Bordetella

  • 53

    What are the other related infections of Non-typeable form of Haemophilus influenzae?

    Conjunctivitis Sinusitis (localized infections)

  • 54

    What is the mode of transmission of Haemophilus influenzae?

    Person-to-person (droplets)

  • 55

    This Haemophilus specie is Non-typeable

    Haemophilus influenzae biogroup aegypticus

  • 56

    No growth of Haemophilus occurs on what medium?

    MacConkey agar

  • 57

    What is the other term for Haemophilus aegypticus?

    Koch-Weeks bacillus

  • 58

    In the Culture of Brucella, isolates can be recovered after ______ but may require prolonged incubation up to ______.

    7 days 30 days

  • 59

    Haemophilus species resemble an______________________ because of their pleomorphic appearances.

    "amorphous serous material"

  • 60

    It is also known as the corroding bacilli

    Eikenella corrodens

  • 61

    It is the preferred medium for Haemophilus because it contains the X and V factors.

    CAP

  • 62

    What media are used in the Culture of Brucella?

    BAP TSA Castaneda's medium

  • 63

    True/False: HACEK group does not causes slow & progressive bacterial endocarditis

    False

  • 64

    What are the most virulent among the species of Brucella?

    Brucella melitensis Brucella suis

  • 65

    It is the agent of chancroid which is highly communicable, sexually transmitted, genital ulcer disease.

    Haemophilus ducreyi

  • 66

    It is the cause of serious infections in humans and leading cause of meningitis in unvaccinated children.

    Haemophilus influenzae type b (Hib)

  • 67

    They are part of the indigenous microbiota & are considered as opportunistic pathogens. They are small, non-motile, cannot grown on MAC.

    HACEK(AACEK) Group

  • 68

    Describe the appearance of brucella under the microscope

    Small coccobacilli that are arranged singly, in pairs which have a “sandy appearance”

  • 69

    It detects the presence of enzymes that converts ALA into porphyrins.

    Porphyrin test

  • 70

    What Serological test is used in Brucella?

    Serum agglutination test

  • 71

    What are the media used for the culture of Haemophilus species?

    CAP BAP BHI Thioglycollate

  • 72

    It is the second prevalent etiologic agent for otitis media with effusion (middle ear infection) after Streptococcus pneumoniae

    Non-typeable form Haemophilus influenzae

  • 73

    It is used for rapid direct identification of the capsular antigens of H. influenzae.

    Neufeld- Quellung reaction

  • 74

    It is the only catalase-positive HACEK specie

    Aggregalibacter actinomycetemcomitans

  • 75

    What is preffered instead of a transport medium for the recovery of Haemophilus ducreyi?

    Plating

  • 76

    What Haemophilus species contain/s x growth factor only?

    Haemophilus ducreyi

  • 77

    Also known as the X factor

    Hemin

  • 78

    This Haemophilus specie is B-haemolytic

    Haemophilus haemolyticus

  • 79

    What are specimens that can be used in the Laboratory diagnosis of Brucella?

    Blood Bone marrow Skin lesions Placental tissues

  • 80

    This category of Haemophilus influenzae does not produce capsules and part of the indigenous microbiota of the URT and adheres to human epithelial cells

    Non-typeable form Haemophilus influenzae

  • 81

    They are non-motile, assacharolytic, and non-encapsulated. Some species require an increased supply of CO2 for growth.

    Brucella

  • 82

    What are the associated infection/disease with Haemophilus aegypticus?

    Pink eye conjunctivitis

  • 83

    When Haemophilus are grown anaerobically, only ____ & not hemin, is required.

    NAD

  • 84

    They are obligate parasites on the mucous membranes of humans. And they die rapidly in clinical specimen and very susceptible to drying and extreme temperatures.

    Haemophilus

  • 85

    What are the two categories of Haemophilus influenzae?

    Typeable form Non-Typeable form

  • 86

    What is the principal Virulence factor of Haemophilus influenzae?

    Polysaccharide capsule

  • 87

    What are the biochemical test and result for Haemophilus?

    (+) catalase (+) oxidase except Haemophilus segnis

  • 88

    All species of Haemophilus normally inhabit the URT of humans except for what?

    Haemophilus ducreyi

  • 89

    The distinguishing characteristic of this Haemophilus specie is School of fish

    Haemophilus ducreyi

  • 90

    What are the human pathogens of the genus Haemophilus?

    Haemophilus influenzae Haemophilus ducreyi Haemophilus parainfluenzae Haemophilus paraphrophilus Haemophilus parahaemolyticus Haemophilus pittmaniae Haemophilus aegypticus Haemophilus segnis

  • 91

    They are assacharolytic like the species of the genus Moraxella and the least common isolate of the HACEK group

    Eikenella corrodens

  • 92

    This Haemophilus specie has mouse/bleach-like odor; non-haemolytic

    Haemophilus influenzae

  • 93

    Also known as the V factor

    NAD

  • 94

    What are the hallmarks of chanroid caused by Haemophilus ducreyi?

    Buboes or suppurative, enlarged, draining, inguinal lymph nodes.

  • 95

    Describe the appearance of Haemophilus ducreyi in CAP

    Colonies are transparent, small, non-mucoid, and tan or yellow.

  • 96

    What are the other Virulence factors of Haemophilus influenzae aside the polysaccharide capsule?

    IgA protease Fimbriae Lipopolysaccharide

  • 97

    It is formerly known as Haemophilus aphrophilus

    Aggregalibacter aphrophilus

  • 98

    What is the selective media for Haemophilus influenzae?

    Horse’s blood-bacitracin agar for respiratory secretions of patients with cystic fibrosis.

  • 99

    Species of this genus are important human and animal pathogens. Obligate aerobes and intracellular parasites.

    Brucella

  • 100

    Haemophilus species grow best at ______ degree cent., except H. ducreyi which grows at ___ degree cent. & in an environment with ___________.

    35-37 35 5% to 10% CO2