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1
What are the species of the family Enterococci?
Enterococcus faecalis, Enterococcus faecium, Enterococcus avium, Enterococcus gallinarum, Enterococcus durans
2
What are diff types of Biochemical test for Enterococci?
Bile esculin, 6.5% NACl test, Acid production
3
Who invented CAMP test?
Christie, Atkins, Munch-Peterson
4
•Upper respiratory infections •Endocarditis, osteomyelitis, brain abscess •Penicillin tolerance •Treat with penicillin and gentamicin •Source of streptokinase used for thrombolytic therapy in patients
Streptococci equisimilis
5
True/False: Prior sensitization is NOT necessary in Acute glomerulonephritis
True
6
• Formerly known as nutritionally variant streptococci (NVS) • Part of human oral and GI microbiota • Culture: colonies appear as "satellites" around organism
Abiotrophia , Granulicatolla
7
In this test, the positive result is swelling of capsule.
Neufel-Quellung reaction
8
It is an indigenous microbiota of human and animal intestinal tract which are not highly pathogenic but are frequent causes of nosocomial infection.
Enterococci
9
Streptococcus bovis group includes:
Streptococcus equinus, Streptococcus gallolyticus, Streptococcus infantarius, Streptococcus alactolyticus
10
What does Enterococci look in Mac Conkey agar?
Tint magenta coloured colonies
11
What are the differential test used in Streptococcus pneumoniae?
Optochin susceptibility test, Bile solubility (Confirmatory test), Neufel-Quellung reaction, Mouse virulence
12
•Site of infection - Throat or skin •Prior sensitisation - not necessary •Serotype 49,53-55,59-61 and 1,12 •Moderate immune response •Complement level - lowered •Genetic susceptibility - Absent •Repeated attacks - Absent •Penicillin prophylaxis is not indicated
Acute glomerulonephritis
13
True/False: Enterococci are NOT resistant to Penicillin
False
14
This streptococcus-like organism is frequently found in plant surfaces, vegetables and in milk products.
Leuconostoc
15
Presence of voluminous fluid hastens the spread of the bacteria to the lungs.
Lobar pneumonia
16
What are the diagnostic markers of Streptococcus agalactiae?
Hippurate hydrolysis, CAMP test
17
This streptococcus-like organism has similar colonial morphology to viridans streptococci.
Gemella
18
What are the Laboratory Diagnosis methods used for Enterococci?
Gram stain, Culture media, Biochemical test , PYR test, Antimicrobial susceptibility test
19
What are the predisposing factors of Lobar Pneumonia?
Alcoholism, Malnutrition, Viral infection of the URT
20
What is the positive result of Bile esculin test for the Laboratory test of Enterococci?
Black colony complex within 48 hours
21
•Present in intestine, genital tract and saliva • It can cause urinary tract infection and wound infection. •Intrinsically resistant to cephalosporins
Enterococci
22
Streptococcus pneumoniae is also known as what?
Diplococcus or pneumococcus
23
Complication of pneumonia and otitis media and commonly affects infants and elderly.
Meningitis
24
What are used for the identification of Enterococci?
Mannitol, Sucrose, Sorbitol fermentation, Bile Esculin hydrolysis
25
What are the different types of Viridans group?
Streptococcus mitis , Streptococcus mutans, Streptococcus salvarius, Streptococcus bovis, Streptococcus anginosus
26
What is the source of Streptococcus agalactiae?
From the maternal vagina during birth
27
Streptococcus mutans group includes:
Streptococcus mutans, Streptococcus sobrinus
28
•Neonatal infection •Most common cause of neonatal meningitis •Source - from the maternal vagina during birth •GBS puerperal sepsis, pneumonia •Diagnostic markers - Hippurate hydrolysis, CAMP test
Streptococcus agalactiae
29
It is also known as minute streptococci
Streptococci MG
30
True/False: Prior sensitization is NOT essential in Acute rheumatic fever.
False
31
Streptococcus anginosus group includes:
Streptococcus anginosus, Streptococcus constellatus, Streptococcus intermedius
32
What are the virulence factor of Strep. pneumoniae?
Capsule, Toxins, Hemolysins, Immunoglobin A protease, Neuraminidase, Hyaluronidase
33
•Grow in the presence of 40% bile •6.5% sodium chloride • pH 9.6 •Growth at 45degC
Enterococci
34
What does Streptococcus pneumoniae look like under gram stain?
Cells appear oval or lancet-shaped
35
•Streptococci normally resident in the mouth and upper respiratory tract •Alpha lysis on blood agar •Cannot be categorised under lancefield antigenic groups •Causes dental caries • Tooth extraction seeding into blood stream endocarditis - hence give prophylactic antibiotics
Viridans group
36
What is the susceptibility test used in Streptococcus pneumoniae?
Optochin disk test
37
What is the positive result of 6.5% NaCl test for the Laboratory test of Enterococci?
Turbidity and growth for the enterococci
38
It is the the source of Streptokinase for thrombolytic therapy in patients.
Streptococci equisimilis
39
•Christie, Atkins and Munch-Peterson •When Strep. agalactiae is inoculated perpendicular to a streak of Staph. aureus grown on blood agar ----> an accentuated zone of hemolysis occurs
CAMP test
40
• Belong to the family Streptococcaceae • Indigenous microbiota of human and animal intestinal tract • Not highly pathogenic but are frequent causes of nosocomial infection • Species: E. faecalis, E. faecium, E. avium, E. gallinarum, E. durans • Most common isolate is E. faecalis • Infections: UTI, endocarditis, bacteremia and wound infection • Laboratory test: Bile esculin and PYR = POSITIVE; Positive growth in 6.5% NaC
Enterococci
41
Streptococcus pneumoniae is the causative agent of _________________.
Lobar pneumonia
42
What will happen if Strep. agalactiae is inoculated perpendicular to a streak of Staph. aureus grown on blood agar?
An accentuated zone of hemolysis occurs
43
• Also known as diplococcus or pneumococcus Causative agent of lobar pneumonia • Most common cause of bacterial meningitis in adults • Cell wall contains antigen called "C substance" • Principal virulence factor is a capsule • Culture: colonies spear flat with depressed centers, mucoid and are alpha-hemolytic
Streptococcus pneumoniae
44
What are common infections caused by Enterococci?
UTI, Endocarditis, Bacteremia, Wound infection
45
This test differentiates pneumococci from viridans streptococci
Bile solubility (Confirmatory test)
46
True/False: Enterococci are resistant to Efromycin acid disk (100 ug).
True
47
How does Abiotrophia and Granulicatolla look like in culture media?
Colonies appear as "satellites" around organism
48
How does Streptococcus pneumoniae looks like in culture media?
Colonies appear flat with depressed centers, mucoid and are alpha-hemolytic
49
What are used for the antimicrobial susceptibility test of Enterococci?
Penicillin, Efromycin acid disk(100 ug)
50
It is the causative agent of lobar pneumonia
Streptococcus pneomoniae
51
In this test, the positive result is the death of the animal.
Mouse virulence
52
What is the positive result of PYR test for the Laboratory test of Enterococci?
Bright pink to cherry red color
53
What is the most common isolate of the family Enterococci?
Enterococcus faecalis
54
Streptococci equisimilis is treat with what?
Penicillin, Gentamicin
55
What is/are the Serotype of Acute glomerulonephritis?
49, 53-55, 59-61 and 1, 12
56
This streptococcus-like organism is a common airborne bacterium which resembles viridans streptococci.
Aerococcus
57
What are the infections and diseases caused by Streptococcus pneumonia?
Lobar pneumonia, Meningitis, Otitis media, Bacteremia, Endocarditis and peritonitis
58
•Site of infection - Throat •Prior sensitization is essential •Serotype - Any •Marked immune response •Unaffected complement level •Genetic susceptibility - present •Repeated attacks - common •Penicillin prophylaxis is essential
Acute rheumatic fever
59
What are the types under the viridans group?
S. mitis, S. mutans, S. salivarius, S. anginosus, S. bovis
60
This test evaluates the ability of S. pneumoniae to lyse in the presence of bile salts
Bile solubility test
61
•Heat resistant - survives 60degC for 30 minutes •Pairs of oval cocci, arranged at an angle to each other •Non hemolytic
Enterococci
62
What are the culture media used in Streptococcus pneumoniae?
CAP, BHI, TSA with 5% sheep's blood
63
What is the treatment for Enterococci?
Penicillin + Aminoglycosides, Vancomycin
64
NOTICE THE DIFFERENCE!!
DONE
65
It is the most common cause of bacterial meningitis in adults
Streptococcus pneumoniae
66
What does Streptococcus pneumoniae looks like in culture media?
Colonies appear "dome-shaped", glistening, mucoid. Old colonies look like coin with a raised rim "dimple-shaped"
67
This test is used to detect the presence of antibody against pneumococci
Francis test
68
Streptococcus salivarius group includes:
Streptococcus salivarius, Streptococcus vestibularis
69
This streptococcus-like organism has resistance to vancomycin that is similar to Leuconostoc.
Pediococcus
70
•Grow poorly on blood agar •Minute streptococci •Streptococcus MG - alphalytic strain - isolated from Primary atypical pneumonia
Streptococcus MG
71
What are the virulence factors of the Viridans group?
Polysaccharide capsule and cytolysin, Extracellular dextran, Adhesins, M protein, Enzymes
72
What are the different Stretococcus-like organisms?
Aerococcus, Gemella, Lactococcus, Leuconostoc, Pediococcus
73
What culture media is used in Laboratory diagnosis for Enterococci?
TSB or BHI with 5% sheep's blood
74
What are the specimens used for the laboratory diagnosis of Enterococci?
Blood, Urine, Wound secretions
75
It is an antigen that is contained in the cell wall of Streptococcus pneumoniae
C substance
76
What are the diff Laboratory diagnosis method for Streptococcus pneumoniae?
Gram stain, Culture Media, Susceptibility test, Bile solubility test, Neufeld-Quellung reaction, Francis test, Coagglutination test
77
This streptococcus-like organism is formerly under the group N. streptococci. And is similar to enterococci but does not produce acid from carbohydrates.
Lactococcus
78
S.pneumonia is the most common isolate in children under 3 years old with recurrent _______________.
Otitis media
79
What is the principal virulence factor of Streptococcus pnemoniae?
Capsule
80
It causes Neonatal infection and is the most common cause of neonatal menigitis
Streptococcus agalactiae
81
Streptococcus mitis group includes:
Streptococcus mitis, Streptococcus pneumoniae, Streptococcus sanguis, Streptococcus oralis