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1
What are the Skin and soft tissue infections caused by Strep. pyogenes?
Cellulitis, Septicemia, Erysipelas, Impetigo
2
It is based on the extraction of C carbohydrate from the streptococcal cell wall.
Lancefield Classification
3
This protein antigen is acid labile, trypsin resistant. Not virulent.
T protein
4
Mostly significant in classifying and identifying B-haemolytic streptococci.
Lancefield Classification
5
Lancefield D is under what hemolytic reaction?
Alpha, Beta, or Gamma-hemolytic
6
Give out the diff. Protein antigens
M protein, T protein, R protein
7
Fammiliarize
Done
8
Give out the Smith and Brown Classification
Alpha-haemolytic streptocci, Beta-haemolytic streptocci, Gamma--haemolytic streptocci
9
Reaction of Family Streptococcaceae
..
10
What is/are the specie/s under Lactic group?
Streptococcus lactis
11
Also called green streptococci
Streptococcis pneumoniae
12
Most common cause of bacterial meningitidis in adults
Streptococcus pnemoniae
13
Indigenous human microbiota, but can cause life-threatening infections if access to normally sterile sites is gained.
Family Streptococcacea
14
What is the adherence of Streptococcus pyogenes in causing Respiratory infection?
By pili
15
What hemolysis does Viridans group have in blood agar?
Alpha lysis
16
- Will neither grow at 10°C nor at 45 °C, but only at 37 C - Mostly has ẞ-haemolytic reactions -S.pyogenes, groups C and G streptocococci
Pyogenic group
17
-Will grow at 10°C to 37°C -Non-haemolytic and has a Lancefield N antigen -Often found in dairy products
Lactic group
18
What is/are the Serotype of Acute rheumatic fever?
Any
19
This specie of B-hemolytic streptococci is bacitracin resistant
Streptococcus agalactiae
20
The new name for Streptococcus MG
Streptococcus anginosus
21
What is the sequelae of Throat infection caused by Strep. pyogenes?
Acute rheumatic fever
22
Describe Streptococcus pyogenes in Blood agar
Small colonies with beta type of hemolysis around them
23
These antigens are used for attachment in epithelial cells
Fimbrial antigens
24
What does MG in Streptococcus MG stands for?
Milleri group
25
-Have partial/complete hemolysis of red blood cells around the colonies -Culture, exhibit a greenish or incomplete hemolysis
Alpha-haemolytic streptococci
26
How do Streptococcus looks like under the microscope?
Gram positive spherical cells that are arranged in chains or pairs
27
Morphology: -Spherical to oval cocci -Arranged in chains, more in liquid medium -Non motile -Non sporing
Streptococcus pyogenes
28
This protein antigen is the most virulent; heat and acid stable, but susceptible to tryptic digestion.
M protein
29
This classification of Streptococci is based on haemolytic pattern.
Smith and Brown classification
30
This specie of B-hemolytic streptococci is bacitracin sensitive
Streptococcus pyogenes
31
It is used for thrombolytic theraphy in patients
Streptokinase
32
-Will grow both at 45 °C and 37°C -Not part of the Lancefield group and maybe a-haemolytic or non-haemolytic -Indigenous microbiota in the upper respiratory tract of humans -Some may have the A, C,G, or N Lancefield antigen
Viridans group
33
Who is the person behind the Lancefield Classification?
Rebecca Lancefield
34
-Will grow at 10 C, 45° C, and 37°C -Part of indigenous microbiota of the human intestines
Enterococcus group
35
This classification of Streptococci is based on temperature requirement.
Academic/Bergey's Classification
36
Species under Lancefield group C
Streptococcus dysgalactiae subsp. equisimilis, Streptococcus equi
37
This protein antigen is not virulent
R protein
38
Specie under Lancefield group A
Streptococcus pyogenes
39
This group is formerly streptococci
Enterococcus group
40
Cultural Characteristic of Streptoccus pyogenes
Done
41
A condition which is the consequence of a previous disease or injury.
Sequelae
42
The antigenic structure of Streptococcus pyogenes are composed of what?
Structural antigens - cell wall antigens, Toxins, Enzymes, Protein antigens , Fimbrial antigens
43
Fill in the blanks
Negative, Positive, Not bile soluble
44
What is primary site of respiratory infections caused by Streptococcus pyogenes?
Throat
45
What are the infections/conditions under suppurative diseases?
Respiratory infection, Skin and soft tissue infections, Genital infections
46
It is a Skin and soft tissue infections caused by Strep. pyogenes which occurs in older patients.
Erysipelas
47
Give out specie/s under Beta-haemolytic streptocci
Streptococcus pyogenes
48
It is an infection of the genital tract occurring at any time between rupture of membrane or labour and 42 days postpartum.
Puerpural sepsis
49
Specie under Lancefield group D
Enterococci
50
This specie of streptococci causes coagulation or souring of milk
Streptococcus lactis
51
Longest chain is produced by Streptococcus _____________ (Commensal)
salivarius
52
What are the species under Pyogenic group?
S. pyogenes, Group C and G Streptococci
53
Give out specie/s under Alpha-haemolytic streptocci
Streptococcus pneomonia (green streptococci), Viridans
54
Some species are ___________ and thus require an increased consumption of CO2 for growth
Capnophilic
55
• M types 1 and 3 forming pyrogenic exotoxin A •High fatality •Flesh eating bacteria •Shock, DIC •Treatment with penicillin - not effective •Vancomycin infections- DOC in life threatening infections
Necrotizing fasciitis
56
Lancefield A is under what hemolytic reaction?
Beta-hemolytic
57
All Streptococcus are facultative anaerobes except for what?
Peptostreprococci
58
What toxin has relation with Necrotizing fascitis?
M type 1 and 3 forming pyrogenic extoxin A
59
What are the toxins and enzymes of Streptococcus pyogenes?
Streptolysin - O, S, Pyrogenic exotoxin, Streptokinase, Deoxyribonucleases (Streptodornase), NADase, Hyaluronidase, Serum opacity factor
60
What is the site of infection of Acute rheumatic fever?
Throat
61
What are the diseases/conditions with non suppurative sequelae?
Acute rheumatic fever, Acute glomerulonephritis
62
All streptococci except the ______________ and _______________ are included in the Lancefield classification.
Viridans group , Streptococcus pneumonia
63
What is/are the specie/s under Enterococcus group?
Enterococcus faecalis
64
Give out the Academuc/Bergey's Classification of Streptococci
Pyogenic group, Viridans group, Lactic group, Enterococcus group
65
How do Streptococcus looks like in Culture Media?
Colonies appear grayish, pinpoint, and translucent to slightly opaque while some species have mucoid colonies.
66
What is the sequelae of Impetigo caused by Step. pyogenes?
Acute glomerulonephritis
67
Specie under Lancefield group B
Streptococcus agalactiae
68
Caused by normal inhabitants of the femal genitalia and an example is Puerperal sepsis.
Genital infections
69
-Exhibit complete lysis of red blood cells around the colony -Culture, clear zone around the colony
Beta-haemolytic streptococci
70
It is the source of Streptokinase used for thrombolytic theraphy in patients
Streptoccus equisimilis
71
Lancefield B and C are under what hemolytic reaction?
Alpha or Gamma hemolytic
72
Do not exhibit the lysis of red blood cells around the colony
Gamma-haemolytic streptococci
73
This condition is caused by what?
Flesh eating bacteria
74
Give out specie/s under Gamma-haemolytic streptocci
Streptococcus bovis, Enterococcus faecalis, Enterococcus faecium
75
It is essential for the treatment of Acute rheumatic fever
Penicillin prophylaxis
76
What are the species under Viridans group
Streptococcus mutans, Streptococcus mitis, Streptococcus salivarus
77
What is this condition?
Cellulitis
78
It is a Skin and soft tissue infections caused by Strep. pyogenes which occurs in Children.
Impetigo
79
How many types are identified in M protein by Griffith typing?
80 types
80
Describe Streptococcus pyogenes in Liquid media
Granular turbidity