Indigenous human microbiota, but can cause life-threatening infections if access to normally sterile sites is gained.

All Streptococcus are facultative anaerobes except for what?

Some species are ___________ and thus require an increased consumption of CO2 for growth

True/False: Growth of Family Streptococcaceae is enhanced by blood, serum or glucose incorporated in the agar plate

All streptococci except the ______________ and _______________ are included in the Lancefield classification.

How do Streptococcus looks like under the microscope?

How do Streptococcus looks like in Culture Media?

Reaction of Family Streptococcaceae

This classification of Streptococci is based on temperature requirement.

- Will neither grow at 10°C nor at 45 °C, but only at 37 C - Mostly has ẞ-haemolytic reactions -S.pyogenes, groups C and G streptocococci

Give out the Academuc/Bergey's Classification of Streptococci

This group is formerly streptococci

What are the species under Pyogenic group?

What are the species under Viridans group

-Will grow both at 45 °C and 37°C -Not part of the Lancefield group and maybe a-haemolytic or non-haemolytic -Indigenous microbiota in the upper respiratory tract of humans -Some may have the A, C,G, or N Lancefield antigen

This specie of streptococci causes coagulation or souring of milk

-Will grow at 10°C to 37°C -Non-haemolytic and has a Lancefield N antigen -Often found in dairy products

What is/are the specie/s under Lactic group?

What is/are the specie/s under Enterococcus group?

-Will grow at 10 C, 45° C, and 37°C -Part of indigenous microbiota of the human intestines

This classification of Streptococci is based on haemolytic pattern.

Give out the Smith and Brown Classification

Give out specie/s under Alpha-haemolytic streptocci

Also called green streptococci

Give out specie/s under Beta-haemolytic streptocci

Give out specie/s under Gamma-haemolytic streptocci

-Have partial/complete hemolysis of red blood cells around the colonies -Culture, exhibit a greenish or incomplete hemolysis

-Exhibit complete lysis of red blood cells around the colony -Culture, clear zone around the colony

Do not exhibit the lysis of red blood cells around the colony

This specie of B-hemolytic streptococci is bacitracin sensitive

This specie of B-hemolytic streptococci is bacitracin resistant

Who is the person behind the Lancefield Classification?

It is based on the extraction of C carbohydrate from the streptococcal cell wall.

Mostly significant in classifying and identifying B-haemolytic streptococci.

Fammiliarize

Lancefield A is under what hemolytic reaction?

Lancefield B and C are under what hemolytic reaction?

Lancefield D is under what hemolytic reaction?

Specie under Lancefield group A

Specie under Lancefield group B

Species under Lancefield group C

Specie under Lancefield group D

Longest chain is produced by Streptococcus _____________ (Commensal)

Morphology: -Spherical to oval cocci -Arranged in chains, more in liquid medium -Non motile -Non sporing

Describe Streptococcus pyogenes in Blood agar

Describe Streptococcus pyogenes in Liquid media

Cultural Characteristic of Streptoccus pyogenes

Fill in the blanks

The antigenic structure of Streptococcus pyogenes are composed of what?

Give out the diff. Protein antigens

These antigens are used for attachment in epithelial cells

How many types are identified in M protein by Griffith typing?

This protein antigen is the most virulent; heat and acid stable, but susceptible to tryptic digestion.

This protein antigen is acid labile, trypsin resistant. Not virulent.

This protein antigen is not virulent

What are the toxins and enzymes of Streptococcus pyogenes?

What are the infections/conditions under suppurative diseases?

What are the diseases/conditions with non suppurative sequelae?

What is primary site of respiratory infections caused by Streptococcus pyogenes?

What is the adherence of Streptococcus pyogenes in causing Respiratory infection?

What is the sequelae of Throat infection caused by Strep. pyogenes?

A condition which is the consequence of a previous disease or injury.

What are the Skin and soft tissue infections caused by Strep. pyogenes?

It is a Skin and soft tissue infections caused by Strep. pyogenes which occurs in older patients.

It is a Skin and soft tissue infections caused by Strep. pyogenes which occurs in Children.

What is the sequelae of Impetigo caused by Step. pyogenes?

What is this condition?

This condition is caused by what?

Caused by normal inhabitants of the femal genitalia and an example is Puerperal sepsis.

• M types 1 and 3 forming pyrogenic exotoxin A •High fatality •Flesh eating bacteria •Shock, DIC •Treatment with penicillin - not effective •Vancomycin infections- DOC in life threatening infections

What is the site of infection of Acute rheumatic fever?

What is/are the Serotype of Acute rheumatic fever?

What toxin has relation with Necrotizing fascitis?

It is an infection of the genital tract occurring at any time between rupture of membrane or labour and 42 days postpartum.

It is essential for the treatment of Acute rheumatic fever

What hemolysis does Viridans group have in blood agar?

Most common cause of bacterial meningitidis in adults

The new name for Streptococcus MG

What does MG in Streptococcus MG stands for?

It is the source of Streptokinase used for thrombolytic theraphy in patients