STAPHYLOCOCCUS AUREUS

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

STAPHYLOCOCCUS General Characteristics

Catalase (+) Non motile Facultative anaerobes Buttery/cream/gold

S.aureus (Aureus latin “?”)

Golden

A.suppurative and pyogenic

S.aureus

NO. 1 CAUSE OF Food poisoning

S.aureus

Food poisoning (no odor/no taste) = Enterotoxin ?

ABD

Enterotoxin ?: Pseudomembranous enterocoliti

Enterotoxin B

Enterotoxin B:

Pseudomembranous enterocoliti

S.aureus IS WHAT Syndrome?

Scalded Skin Syndrome

Enterotoxin ?:Toxic Shock Syndrome (TSS)

BCGI

Enterotoxin BCGI:

Toxic Shock Syndrome (TSS)

: unknown cause linked to infection and vaccine

Toxic Epidermal Necrolysis (TEN)

S.aureus IS CLUSTERS OF?

GRAPES

Scalded Skin Syndrome AKA

RITTERS DISEASE

AN EXFOLIATIVE DERMATITIS/ PRONE TO KIDNEY FAILURE

Scalded Skin Syndrome AKA RITTERS DISEASE

Scalded Skin Syndrome AKA RITTERS DISEASE is an and prone to?

AN EXFOLIATIVE DERMATITIS/ PRONE TO KIDNEY FAILURE

MULTISYSTEM FAILURE, SHOCK HYPOTENSION DIC, DEATH

Enterotoxin BCGI:Toxic Shock Syndrome (TSS).

DSE secondary to Influenza

Pneumonia

Pneumonia secondary to

Influenza

Osteomyelitis secondary to ____________

BACTEREMIA

secondary to BACTEREMIA

Osteomyelitis

Coagulase =

MAJOR VIRULENCE FACTOR; CLOT PRODUCTION

= MAJOR VIRULENCE FACTOR; CLOT PRODUCTION

Coagulase

= BINDS TO FC PORTION OF IMMUNOGLOBULIN

Protein A

Hyaluronidase = _____________ factor

spreading

= spreading factor

Hyaluronidase

Alpha and beta hemolysin = hemolysis of RBCs wc leads to?

hemolytic anemia

B hemolysin: ? – Hot-Cold hemolysin

Sphingomyelinase C

: Sphingomyelinase C – Hot-Cold hemolysin

B hemolysin

B hemolysin: Sphingomyelinase C – Hot-Cold hemolysin, _____________ (+) to ID Group B strep

CAMP

it is B hemolysin: Sphingomyelinase C – Hot-Cold hemolysin, CAMP (+) to ID Group B strep

S.aureus

Gamma hemolysin: Assoc.to _____________

PANTON VALENTIN LEUKOCIDIN

: Assoc.to PANTON VALENTIN LEUKOCIDIN

Gamma hemolysin

Assoc to _____________ Acquired Pneumoniae

COMMUNITY

Assoc to COMMUNITY Acquired Pneumoniae (CAUSATIVE AGENT)

S.Pneumoniae

is produced by all strains of S. aureus and may actually function in association with the Panton-Valentine leukocidin (PVL)

Gamma toxin

Gamma toxin is produced by all strains of S. aureus and may actually function in association with the ?

Panton-Valentine leukocidin (PVL)

Thirty to fifty percent of all S. aureus strains are capable of producing one of eight distinct serologic types of a heat-_____________ enterotoxin

STABLE

Thirty to fifty percent of all S. aureus strains are capable of producing one of ? distinct serologic types of a heat-STABLE enterotoxin

eight

? percent of all S. aureus strains are capable of producing one of eight distinct serologic types of a heat-STABLE enterotoxin

Thirty to fifty

The enterotoxins are resistant to hydrolysis by the gastric and intestinal enzymes. The toxins, which are often found in _____________ products, are associated with pseudomembranous ENTEROCOLITIS and TOXIC shock syndrome, and they may exacerbate the normal immune response, resulting in further tissue damage and systemic pathology

MILK

The enterotoxins are resistant to hydrolysis by the gastric and intestinal enzymes. The toxins, which are often found in MILK products, are associated with ? and , and they may exacerbate the normal immune response, resulting in further tissue damage and systemic pathology

pseudomembranous ENTEROCOLITIS , TOXIC shock syndrome

S.aureus ON BA

BETAHEMOLYTIC

= facilitates colonization on skin (CARBUNCLES)

Lipase

= binds and inactivates B-lactams antibiotics

B-lactamase

=FOUND IN REUSABLE TOURNIQUET

Methillicin Resistant

S.aureus RESISTANT IN?

Methillicin Resistant

S. AUREUS IN BA AND CNA COLONIES:

MEDIUM TO LARGE RAISED CREAM TO GOLDEN YELLOW

IN BA AND CNA COLONIES: MEDIUM TO LARGE RAISED CREAM TO GOLDEN YELLOW

S. AUREUS

S. AUREUS FREE COAGULASE TUBE (+/-)

FREE COAGULASE TUBE POSITIVE

S. AUREUS BOUND COAGULASE / CLUMPING FACTOR (+/-)

BOUND COAGULASE / CLUMPING FACTOR POSITIVE

S. AUREUS DNASE (+/-)

DNASE POSITIVE

FORMER NAME OF MRSA

OXALINE RESISTANT STAPHYLOCOCCUS AUREUS

FORMER NAME OXALINE RESISTANT STAPHYLOCOCCUS AUREUS

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

RESISTANT TO ALL BETA LACTAM ANTIBIOTICS

S.aureus

LEADING CAUSE OF HOSPITAL ACQUIRED INFECTIONS

MRSA

TRANSPEPTIDASE PB2A

MECA GENE

TRANSPEPTIDASE PB2A

= inhibit B-lactamase

Clavunilic Acid

_____________ gene: Codes for MRSA,

MECA

MECA gene: Codes for ,

MRSA

: Codes for MRSA, GOLD STD FOR MRSA IDENTIFICATION

MECA gene

_____________ – most common treatment for MRSA

VANCOMYCIN

RESISTANT TO NAFCILLIN

VANCOMYCIN

VANCOMYCIN RESISTANT TO

NAFCILLIN

BOUND TO CYTOPLASMIC MEMBRANE OF ORGANISM AND HAS HIGH AFFINITY FOR THE FC RECEPTOR IgG molecules and complement

protein a

PBP TARGETS OF BETA LACTAM ANTIBIOTICS SUCH AS

PENICILLINS AND CEPHALOSPORINS

RESISTANT SCREENING FOR MRSA

CEFOXITIN DISK DIFFUSION TEST

INDUCIBLE RESISTANCE TO CLINDAMYCIN BY ERYTHROMYCIN D SHAPED ZONE OF INHIBITION AROUND CLINDAMYCIN RISK

D ZONE TEST (D- TEST)

S.aureus Collection: / BEST SAMPLE

ASPIRATE

= mannitol and 7% NaCl

MSA

MSA = combination of? and ?

mannitol and 7% NaCl

Mannitol + Org –fermentation-- > Acid w/c turns

pink to yellow (yellow halo)

Uses ? as Carb source: S.aureus

Mannitol

= Kills gram (-)

Polyethyl alcohol (PEA), and Columbia-Nalidixic acid agar (CNA)

Polyethyl alcohol (PEA), and Columbia-Nalidixic acid agar (CNA) = Kills gram (?)

gram (-)

PEA-is helpful when a specimen is contaminated w/_____________ and if it’s a stool sample

proteus

PEA-is helpful when a specimen is contaminated w/proteus and if it’s a ? sample

stool

-is helpful when a specimen is contaminated w/proteus and if it’s a stool sample

PEA

– selective and differential for S.aureus

CHROMagar

CHROMagar – for S.aureus

selective and differential

CHROMagar – selective and differential for

S.aureus

clear zone of hemolysis

B-hemolytic

Slide:Cell bound – org + Rabbit plasma =

clump

Tube: Free – org + 0.5mL rabbit plasma –incubation->37oC for 4hours =

clot

: Free – org + 0.5mL rabbit plasma –incubation->37oC for 4hours =

Tube

:Cell bound – org + Rabbit plasma =clump

Slide

Macrolide resistance (D Test)

clindamycin resistance

Macrolide resistance (D Test) clindamycin resistance induced by

erythromycin

used to screen mrsa

cefoxitin disk

for confirmation agar dilution broth microdilution

DILUTION METHOD

SIMILAR TO MIC

E-TEST METHOD

for detection of bacteria

VITEK2 SYSTEM

SUPER ANTIGENS BINDS TO MHC II WHICH ACTIVATES T CELL STIMULATION

TOXIC SHOCK SYNDROME TSST-1

ACTS AS EUKARYOTIC EUKARYOTC CELL MEMBRANE

a-hemolysin

degrades sphingomyelin and rbc resulting cell lysis

B-toxin

disrupts biologic membranes, and may have a role in diarrheal diseases, demonstrates non-specific membrane toxicity to other mammalian cells

delta toxin

association with the Panton Valentine Leukocidin (PVL) , a leucocidin, lyses, wbc severe inflamation

gamma hemolysin

kill wbc by disrupting cell membranes

Panton Valentine Leukocidin (PVL)

heat stable &resistant to the action of gut enzymes causes of food poisoning are produced when s. aureus growns in carbohydrates and protein foods

enterotoxin

100

A STAPHYLOCOCCUS WHICH CAUSES A DOG BITE INFECTION

STAPHYLOCOCCUS INTERMEDIUS

other names parasitology

other names parasitology

70問 • 2年前

Yves Laure Pimentel · 111問 · 2年前

PARASITOLOGY

PARASITOLOGY

111問 • 2年前

Yves Laure Pimentel · 64問 · 2年前

HTMLBE

HTMLBE

64問 • 2年前

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CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

61問 • 2年前

Yves Laure Pimentel · 69問 · 2年前

HISTOPATHOLOGY

HISTOPATHOLOGY

69問 • 2年前

CC-2 SPECTROPHOTOMETRY

CC-2 SPECTROPHOTOMETRY

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CC-3 ELECTROPHORESIS

CC-3 ELECTROPHORESIS

41問 • 2年前

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AUBF LAB SAFETY

AUBF LAB SAFETY

87問 • 2年前

CSF 1

CSF 1

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CSF 2

CSF 2

82問 • 2年前

SEMEN 1

SEMEN 1

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SEMEN 2

SEMEN 2

7問 • 2年前

SYNOVIAL FLUID 1

SYNOVIAL FLUID 1

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SYNOVIAL FLUID 2

SYNOVIAL FLUID 2

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SEROUS FLUID

SEROUS FLUID

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PLEURAL FLUID

PLEURAL FLUID

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PERICARDIAL FLUID

PERICARDIAL FLUID

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PERITONEAL FLUID/ ASCITIC FLUID

PERITONEAL FLUID/ ASCITIC FLUID

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AMNIOTIC FLUID

AMNIOTIC FLUID

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FECALYSIS

FECALYSIS

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OTHER BODY FLUIDS

OTHER BODY FLUIDS

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MTLBE- SUHO NOTES

MTLBE- SUHO NOTES

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MTLBE..

MTLBE..

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CC- CHROMATOGRAPHY

CC- CHROMATOGRAPHY

19問 • 1年前

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CC-OSMOMETRY

CC-OSMOMETRY

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CC-ELECTROCHEMISTRY TECHNIQUES

CC-ELECTROCHEMISTRY TECHNIQUES

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CC- CARBOHYDRATES

CC- CARBOHYDRATES

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CC- DIABETES MELLITUS

CC- DIABETES MELLITUS

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MAJOR LIPOPROTEINS

MAJOR LIPOPROTEINS

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MINOR LIPOPROTEINS

MINOR LIPOPROTEINS

63問 • 2年前

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PROTEINS

PROTEINS

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PLASMA PROTEINS 1

PLASMA PROTEINS 1

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PLASMA PROTEINS 2 (ALPHA 2 REGION)

PLASMA PROTEINS 2 (ALPHA 2 REGION)

98問 • 2年前

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MISCELLANEOUS PROTEINS

MISCELLANEOUS PROTEINS

61問 • 2年前

Liver Function Test 1

Liver Function Test 1

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Liver Function Test 2

Liver Function Test 2

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TUMOR MARKERS

TUMOR MARKERS

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KIDNEY FUNCTION TESTS - NON PROTEIN NITROGEN & CREATININE

KIDNEY FUNCTION TESTS - NON PROTEIN NITROGEN & CREATININE

41問 • 2年前

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GLYCOGEN STORAGE DISEASES

GLYCOGEN STORAGE DISEASES

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LIPID STORAGE DISEASES

LIPID STORAGE DISEASES

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PROTEINS

PROTEINS

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QUICK FIRE- ELECTRODES

QUICK FIRE- ELECTRODES

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ELECTROLYTES

ELECTROLYTES

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DRUGS

DRUGS

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TOXIC AGENTS:

TOXIC AGENTS:

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HEMATOLOGY 1 (HEMATOPOIESIS 1)

HEMATOLOGY 1 (HEMATOPOIESIS 1)

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HEMATOLOGY (HEMATOPOIESIS 2)

HEMATOLOGY (HEMATOPOIESIS 2)

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hema diseases

hema diseases

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AML

AML

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GLOBIN SYNTHESIS

GLOBIN SYNTHESIS

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HEMATOLOGY REFERENCE RANGES

HEMATOLOGY REFERENCE RANGES

31問 • 2年前

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DISORDERS OF PRIMARY HEMOSTASIS

DISORDERS OF PRIMARY HEMOSTASIS

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PLATELET DISORDERS

PLATELET DISORDERS

37問 • 2年前

CHARACTERISTICS OF CLOTTING FACTORS

CHARACTERISTICS OF CLOTTING FACTORS

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4. CLASSIFICATION OF MACROPHAGE

4. CLASSIFICATION OF MACROPHAGE

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3. NATURAL OR INNATE IMMUNITY

3. NATURAL OR INNATE IMMUNITY

39問 • 2年前

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5. INTERNAL DEFENSE:SOLUBLE FACTORS (ACUTE PHASE REACTANTS)

5. INTERNAL DEFENSE:SOLUBLE FACTORS (ACUTE PHASE REACTANTS)

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6. PHAGOCYTOSIS Chemotaxis

6. PHAGOCYTOSIS Chemotaxis

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8. NATURE OF ANTIGEN

8. NATURE OF ANTIGEN

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9. MAJOR HISTOCOMPATIBILITY COMPLEX/HUMAN LEUKOCYTE ANTIGEN

9. MAJOR HISTOCOMPATIBILITY COMPLEX/HUMAN LEUKOCYTE ANTIGEN

50問 • 2年前

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1. HISTORY

1. HISTORY

69問 • 2年前

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2. TYPES OF IMMUNITY

2. TYPES OF IMMUNITY

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7. PHAGOCYTOSIS Engulfment and Digestion

7. PHAGOCYTOSIS Engulfment and Digestion

21問 • 2年前

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8. PHAGOCYTOSIS EXOCYTOSIS

8. PHAGOCYTOSIS EXOCYTOSIS

15問 • 2年前

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10. DISEASE ASSOCIATED TO HUMAN LEUKOCYTE ANTIGEN (henry)

10. DISEASE ASSOCIATED TO HUMAN LEUKOCYTE ANTIGEN (henry)

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11. TRANSPLANTATION IMMUNOLOGY

11. TRANSPLANTATION IMMUNOLOGY

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2. LYMPHOID ORGANS

2. LYMPHOID ORGANS

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3. CLUSTER OF DIFFERENTIATION

3. CLUSTER OF DIFFERENTIATION

48問 • 2年前

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4. T-CELL DIFFERENTIATION

4. T-CELL DIFFERENTIATION

35問 • 2年前

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5. T CELL IMMUNODEFICIENCIES

5. T CELL IMMUNODEFICIENCIES

18問 • 2年前

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6. STAGES IN B-CELL DIFFERENTIATION  PROPIMAP

6. STAGES IN B-CELL DIFFERENTIATION  PROPIMAP

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7. B CELL IMMUNODEFICIENCIES

7. B CELL IMMUNODEFICIENCIES

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8. COMBINED T CELL AND B CELL DEFICIENCIES

8. COMBINED T CELL AND B CELL DEFICIENCIES

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9. COMPARISON OF T AND B CELLS

9. COMPARISON OF T AND B CELLS

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10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

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11. NATURAL KILLER CELLS OR THIRD POPULATION CELL

11. NATURAL KILLER CELLS OR THIRD POPULATION CELL

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12. ANTIBODY

12. ANTIBODY

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13. TYPES OF ANTIBODIES

13. TYPES OF ANTIBODIES

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14. MONOCLONAL ANTIBODIES

14. MONOCLONAL ANTIBODIES

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1. INTERLEUKINS

1. INTERLEUKINS

23問 • 2年前

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2. INTERFERONS

2. INTERFERONS

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3. CYTOKINES IN THE INNATE AND ADAPTIVE IMMUNITY

3. CYTOKINES IN THE INNATE AND ADAPTIVE IMMUNITY

8問 • 2年前

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4. COMPLEMENT SYSTEM

4. COMPLEMENT SYSTEM

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15. COMPLEMENT SYSTEM

15. COMPLEMENT SYSTEM

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16. SYSTEM CONTROLS/COMPLEMENT REGULATION

16. SYSTEM CONTROLS/COMPLEMENT REGULATION

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17. COMPLEMENT AND DISEASE STATES

17. COMPLEMENT AND DISEASE STATES

29問 • 2年前

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18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

19問 • 2年前

Yves Laure Pimentel · 22問 · 2年前

19. CYTOKINES

19. CYTOKINES

22問 • 2年前

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20. INTERLEUKINS

20. INTERLEUKINS

24問 • 2年前

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21. INTERFERONS, TNF, TGF, CHEMOKINE

21. INTERFERONS, TNF, TGF, CHEMOKINE

21問 • 2年前

Yves Laure Pimentel · 54問 · 2年前

1. SERO

1. SERO

54問 • 2年前

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2. PRECIPITATION

2. PRECIPITATION

38問 • 2年前

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3. PASSIVE IMMUNODIFFUSION

3. PASSIVE IMMUNODIFFUSION

17問 • 2年前

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4. OUCHTERLONY

4. OUCHTERLONY

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5. ELECTROPHORETIC TECHNIQUE

5. ELECTROPHORETIC TECHNIQUE

9問 • 2年前

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6. IMMUNOELECTROPHORESIS (DOUBLE DIFFUSION)

6. IMMUNOELECTROPHORESIS (DOUBLE DIFFUSION)

11問 • 2年前

Yves Laure Pimentel · 29問 · 2年前

7. IMMUNOFIXATION ELECTROPHORESIS

7. IMMUNOFIXATION ELECTROPHORESIS

29問 • 2年前