Side –Chain theory STIMULATED BY?

theory which Cells have specific receptor for antigen before exposure

theory for Lock and key

it laid the foundation for further hypotheses.

Clonal Selection Discovered by?

Niels Jerne and Macfarlane Burnet discovered

what do you call where Each lymphocyte is preprogrammed to prod. 1 type of Ig and specific Ag selects them. 3 to 5 days proliferation

Accounts for most of the basic properties of the adaptive immune system

Constant and variable region have separated genes for coding discovered by

Who Discovered that chromosomes contain no intact gene for Ig, only bldg. blocks from w/c gene can be assembled

Discoverers -ANTIBODY

ANTIBODY Heavy Chain -CHROMOSOME #

ANTIBODY Light Chains -CHROMOSOME #

ANTIBODY Light Chains -2 and 22 2 types

Sensitive indicator of a monoclonal gammopathy

Serum Free Light Chain Found in

Serum Free Light Chain MEASURED BY rate or endpoint (also heavy chain)

Bence Jones Protein Discoverer:

Dr.Henry Bence-Jones Discovered?

light chains secreted by malignant Plasma cells

found in urine of patient with MULTIPLE MYELOMA

Bence Jones Protein found in urine of patient with

Bence Jones Precipitate at ? at dissolve at ?

ANTIBODY Location

ANTIBODY Functions:

ANTIBODY Composes:

2 heavy chain and 2 light chain

ANTIBODY Mostly are ________ charge:

ANTIBODY – linking protein

ANTIBODY – Cleavage with

Has NO antigen binding ability

Fc/Fragment Crystallizable (at ?)

(at 4C)

Important in effector function of Ig

CLEAVE IN TO 2 PARTS

Pepsin CLEAVE IN TO 2 PARTS?

CLEAVED AB INTO 3 PARTS OR FRAGMENTS

Papain CLEAVED AB INTO 3 PARTS OR FRAGMENTS?

complement C1q binding portion

Fc/Fragment Crystallizable Represents ? -terminal

Location: BETWEEN Ch1 and Ch2

Hinge Region Location:

Hinge Region has high content of ________ for FLEXIBILITY

has high content of PROLINE for FLEXIBILITY

Hinge Region Enhance functional activity of _______

Enhance functional activity of FC

fix the complement

FC BINDING PORTION

Important in effector function of Ig ARE:

IIMMUNOGLOBULINS IN HINGE REGION

BEST IMMUNOGEN

MADE FROM AMINO ACID

Fab Fragment AKA

COMBINING SITE

Fab Fragment (Fragment antigen-binding) ________ acid terminal

AMINO acid terminal

Fab Fragment (Fragment antigen-binding) Fd Fragment

Fab Fragment (Fragment antigen-binding) IN WHAT FRAGMENT?

2 Regions

Variable region CONSIST OF

VARIABLE LIGHT CHAIN AND VARIABLE HEAVY CHAIN IS IN WHAT REGION?

CONSTANT HEAVY CHAIN AND CONSTANT LIGHT CHAIN

Constant region CONSISTS OF

1 LIGHT CHAIN AND 1/2 HEAVY CHAIN

FD FRAGMENT CONSIST OF

VARIABLE REGION OF LIGHT CHAIN AND HEAVY CHAIN

Antibody Variations are (3)

Same heavy chain for each class

Variations in constant region

Variations in variable regions that give individual antibody molecules specificity

Antibody Phases

Ab catabolism

Decline

Ab stabilizes

Plateau

Log phase –

– Ab increase

Lag phase –

– No Ab detectable