3. PASSIVE IMMUNODIFFUSION

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

precipitation on support medium

PASSIVE IMMUNODIFFUSION

Factors affecting diffusion: size of particles, temp, gel viscosity, and hydration

PASSIVE IMMUNODIFFUSION

PASSIVE IMMUNODIFFUSION Factors affecting diffusion:

size of particles, temp, gel viscosity, and hydration

(Single diffusion, single dimension)

Radial Immunodiffusion

Radial Immunodiffusion (?)

Single diffusion, single dimension

Radial Immunodiffusion DISCOVERED (?)

James Oudin

RESULTS IN PRECIPITIN LINE

Radial Immunodiffusion

Modification of RID Ab (well) + Ag (sample) = precipitin_________ = Conc.

RING

? Ab (well) + Ag (sample) = precipitin RING = Conc.

Modification of RID

: Endpoint - Square of the diameter= Conc.

Mancini

Antigen is allowed to diffuse to COMPLETION

Mancini

in mancini method: Antigen is allowed to diffuse to COMPLETION HOW MANY HRS?

24 TO 72 H

– Diameter = log of Conc.

Fahey and Mckelvey/kinetic method

Fahey and Mckelvey/kinetic method – Diameter =

log of Conc.

uses measurements taken before the point of equivalence is reached.

Fahey and Mckelvey/kinetic method

Modification of RID used to measure

IgG, IgM, IgA, and complement components

used to measure IgG, IgM, IgA, and complement components

Modification of RID

other names parasitology

other names parasitology

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