7. IMMUNOFIXATION ELECTROPHORESIS

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

Immunofixation electrophoresis DISCOVERED BY

Alper and Johnson

IT is fixed on gel’s surface -> Shorter time

Antisera

Antisera is fixed on gel’s surface ->

Shorter time

-> Antiserum is applied directly to the gel’s surface

Electrophoresis

Electrophoresis -> ? is applied directly to the gel’s surface

Antiserum

Antibody applied: a. antibody to gamma, alpha, or mu heavy chains and to kappa or lambda light chains (5 lanes)

Immunofixation electrophoresis

sixth lane is overlaid with antibody to _________ serum proteins and serves as the _________ lane

ALL; REFERENCE

is overlaid with antibody to ALL serum proteins and serves as the REFERENCE lane

sixth lane

Hypogammaglobulinemias - _________ staining bands

FAINT

- FAINT staining bands

Hypogammaglobulinemias

polyclonal hypergammaglobulinemias - _________ staining bands in the gamma

DARKER

- DARKER staining bands in the gamma

polyclonal hypergammaglobulinemias

Monoclonal bands, such as found in Waldenström’s macroglobulinemia or multiple myeloma - _________ dark band

NARROW

, such as found in Waldenström’s macroglobulinemia or multiple myeloma - NARROW dark band

Monoclonal bands

Monoclonal bands, such as found in ? - NARROW dark band

Waldenström’s macroglobulinemia or multiple myeloma

Dense wide bands in both the κ and λ lanes, No bands present in heavy chain lanes: Increased ____ Fab fragments

POLYCLONAL

____ in both the κ and λ lanes, No bands present in heavy chain lanes: Increased POLYCLONAL Fab fragments

Dense wide bands

Heavy wide bands seen with both anti-κ and anti-λ antisera indicate excessive _________-chain excretion

LIGHT

seen with both anti-κ and anti-λ antisera indicate excessive LIGHT -chain excretion

Heavy wide bands

adapted by WESTERN BLOT

Immunofixation electrophoresis

Immunofixation electrophoresis adapted by

WESTERN BLOT

is technically simpler and less subject to antigen excess

IEP Immunoelectrophoresis

high concentrations of monoclonal protein with IFE give no _________ reactions, whereas IEP is considered to be a better technique for typing _________ monoclonal gammopathies

VISIBLE; LARGE

high concentrations of monoclonal protein give no VISIBLE reactions,

IFE

is considered to be a better technique for typing LARGE monoclonal gammopathies

IEP

can be optimized for greater sensitivity

IFE

IFE results are easy to _________ than IEP

interpret

results are easy to interpret

IFE

IFE is for _________ proteins

other names parasitology

other names parasitology

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