1. SERO

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

Plain evacuated tube (serum) – ? complete clot retraction.

1hr

tube?– 1hr complete clot retraction.

Plain evacuated tube (serum)

centrifuged for ? minutes

remedy– serum contaminated with erythrocytes

Recentrifuged

Recentrifuged – serum contaminated with ?

erythrocytes

HEAT

coagulates protein

HEAT-

coagulates protein

BACTERIAL growth –

alters protein

– alters protein.

BACTERIAL growth

LIPEMIA

– interference

LIPEMIA

ACID/ ALKALI =

Denature protein

= Denature protein

ACID/ ALKALI

= sample valid on some test

Icteric/Turbid serum

= Freeze at -20oC

>72 hours specimen

>72 hours specimen =

Freeze at -20oC

>72 hours specimen

Freeze at -20oC

process that DESTROYS complement

Complement Inactivation

promote lysis of the red blood cells

Complement Inactivation

= syphilis interference

Serum Complement

False positive (?)

latex passive agglutination and Hemagglutination

result? (latex passive agglutination and Hemagglutination)

False positive

Physical = heat 56oC for 30 min. for _________ hours elapsed -> 56oC for _________.

4, 10 mins

= heat 56oC for 30 min.

PHYSICAL

for 4 hours elapsed -> heated at? for?

56oC for 10

Physical = heat ?

56oC for 30 min.

= choline chloride and thimerosal

Chemical

Chemical =

choline chloride and thimerosal

– used if great accuracy is not required.

GRADUATED

recognized by frosted ring at noncalibrated end, with calibrations extending tip

SEROLOGIC

Greatest accuracy – used _________ pipette.

SMALL

– used SMALL pipette.

Greatest accuracy

is the greatest potential hazard

mouth pipetting

The _________ is the curvature in the top surface of a liquid

meniscus

–The most common type of micropipette used in many laboratories is one that is automatic or semiautomatic

Micropipettor

- material being diluted

Solute

– the medium making up the rest of the solution

Diluent

Simple Dilution The relationship between these two is expressed as a

fraction

- is an indication of relative concentration

DILUTION

– used to correct for having used a diluted sample in a determination rather than the undiluted sample

Dilution factor

Specimen dilution: Simple Dilution FORMULA

1/Dilution = SOLUTE/TOTAL VOL (SOLUTE+DILUENT)

dilution occurs in several stages

Compound Dilution

what do you call? 1:500 dilution can be achieved by making a 1:5 dilution of the original serum, a 1:10 dilution from the first dilution, and another 1:10 dilution

Compound Dilution

Multiplying 5 x 10 x 10 equals 500, or the total dilution

Compound Dilution

If, in each step of the dilution, the dilution factor is exactly the SAME

Serial dilution

a doubling dilution, in which the amount of serum is cut in half with each dilution

Serial dilution

The most common serial dilution is a ?, in which the amount of serum is cut in half with each dilution

doubling dilution

Antibody Test – blood should be drawn during the _________ phase of illness

ACUTE

– blood should be drawn during the ACUTE phase of illness

Antibody Test

Antibody Test – blood should be drawn during the ACUTE phase of illness – when the disease is first discovered or suspected and another sample drawn during the _____(2 weeks later)

convalescent phase

Antibody Test – blood should be drawn during the ACUTE phase of illness – when the disease is first discovered or suspected and another sample drawn during the convalescent phase (weeks?)

2 weeks later

- levels of antibody.

Titer

Titer - levels of antibody. Reciprocal of the _________ dilution of the patient’s serum in which the Ab is still _________.

HIGHEST, DETECTABLE

Reciprocal of the HIGHEST dilution of the patient’s serum in which the Ab is still DETECTABLE.

Titer

other names parasitology

other names parasitology

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