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SEMEN 1
100問 • 2年前
  • Yves Laure Pimentel
    • 通報

    問題一覧

  • 1

    Paired glands inside the scrotum and contain semineferous glands

    TESTES

  • 2

    site of spermatogenesis

    semineferous glands

  • 3

    production of spermatozoa and located in epithelial cells of seminiferous tubules

    GERM CELLS

  • 4

    provide support & nutrients for the germ cells as they undergo spermatogenesis => complete > Immature sperm enter EPIDIDYMIS where sperm mature & develops flagella & stored until ejaculation

    SORTOLI CCELLS

  • 5

    propels sperm to ejaculatory duct w/ the fluid from seminal vesicles

    VAS DEFERENS

  • 6

    produce most fluid in semen (60-70%)- transport fluid of sperm

    SEMINAL VESICLE

  • 7

    produce most fluid in semen ____- transport fluid of sperm

    (60-70%)

  • 8

    Energy for flagella)

    FRUCTOSE

  • 9

    (Gray appearance of the semen)

    FLAVIN

  • 10

    produces 20-30% of semen volume (acidic)

    PROSTATE GLAND

  • 11

    produces ___ of semen volume (acidic)

    20-30%

  • 12

    Differs from other fluids

    ACID PHOSPHATASE

  • 13

    DECREASE amount: Disorders of prostate

    ZINC

  • 14

    controls liquefaction & coagulation of seminal fluid

    PROTEOLYTIC ENZYMES

  • 15

    antibacterial

    SPERMINE AND CHOLINE

  • 16

    produces 5% of the fluid volume composed of thick..alkaline mucus

    BULBOURETHRAL GLANDS

  • 17

    BULBOURETHRAL GLANDS produces how many % of the fluid volume composed of thick..alkaline mucus

    5%

  • 18

    Neutralize the acidity from the prostatic secretion & vagina

    ALKALINE MUCUS

  • 19

    ALKALINE MUCUS: Neutralize the acidity from the prostatic secretion & vagina w/o this neutralization:

    Motility is diminished

  • 20

    Sexual abstinence:

    Atleast 2 days but not > 7 days

  • 21

    WHO fertility testing: ? samples (? intervals)

    2-3; 2-weekS

  • 22

    liquefaction:

    30 min.-1 hr

  • 23

    best Method of Collection:

    MASTURBATION

  • 24

    Method of Collection Not reliable = 1# portion of ejaculate is loss 2 | sperm count; low pH: affects motility

    Coitus Interruptus/ Withdrawal method

  • 25

    aspiration of semen from vaginal vault after coitus

    Vaginal Vault Aspiration

  • 26

    Non-lubricant rubber/ Poly-urethane condoms; Ordinary condom: spermatocides

    Condom Method:

  • 27

    ALL SPX ARE POTENTIAL SOURCE OF

    HIV/ HBV

  • 28

    Preservation o @?: Transport

    RT

  • 29

    Body temp (?): While awaiting analysis

    37C

  • 30

    Fructose level: tested w/n ? For frozen fo prevent FRUCTOLYSIS -85 DEGREES CELCIUS

    2 HOURS

  • 31

    Fructose level: tested w/n 2 HOURS For frozen fo prevent ?

    FRUCTOLYSIS -85 DEGREES CELCIUS

  • 32

    SEMEN ANALYSIS = Appearance o Normal Color:

    GRAY-WHITE (Translucent/ Pearly-white)

  • 33

    SEMEN Odor:

    Musty odor, Fishy, BLEACH LIKE/ CLOROX LIKE

  • 34

    Clear specimen:

    DECREASED sperm conc.: infertility

  • 35

    COLOR: Urine contamination, antibiotics, prolonged abstinence, pyospermia

    Yellow

  • 36

    If after 2 hrs, the specimen not liquefied: add:

    DULBECCOS PHOSPHATE BUFFERED SALINE Proteolytic enzymes: A- CHYMOTRYPSIN & BROMELAIN

  • 37

    : Liquefied specimen

    Jelly-like granules (gelatinous bodies)

  • 38

    Volume o Normal: semen

    2-5 ml

  • 39

    Refers to the consistency of the fluid

    Viscosity

  • 40

    Incomplete liquefied specimen:

    clumped & highly viscous

  • 41

    Droplets that forms thread longer than 2 cm:

    ABNORMAL/ HIGLY VISCOUS

  • 42

    Viscosity IS 0

    WATERY

  • 43

    Viscosity IS 4

    GEL LIKE

  • 44

    Indicates the balance bet. from the acidic prostatic secretion & alkaline seminal vesicles secretion

    pH

  • 45

    Normal pH:

    7.2 - 8.0

  • 46

    increased pH:

    Reproductive tract infection

  • 47

    decreased pH:

    increased prostatic fluid, ejaculatory duct obstruction, poorly developed seminal vesicles

  • 48

    Normal Sperm Concentration:

    20-250 million/ mL

  • 49

    Sperm Concentration & Sperm Count Borderline:

    10-20 million/ mL

  • 50

    Sperm Concentration & Sperm Count USING

    NEUBAUER COUNTING CHAMBER

  • 51

    diluting fluids: (both immobilize & preserve cells)

    SODIUM BICARBONATE, FORMALIN

  • 52

    can also be used as Diluting fluids:

    SALINE, distilled H20 (cold) or Tap H20

  • 53

    inflammation/ infection w/n reproductive tract

    1 million leukocyte/ml:

  • 54

    Spermatogenesis disruption, viral infections, toxic chemicals

    1 million spermatids/ml

  • 55

    undiluted spx, sperms are immobilized by heating, sperm motility is assessed unheated portion

    MAKLER

  • 56

    Complete/ total/Absence of spermatozoa seen

    AZOOSPERMIA

  • 57

    presence of sperm cells whether completely dead or immobile

    NECROSPERMIA

  • 58

    Deficiency in the number of sperm cells or presence of few motile cells seen

    OLIGOSPERMIA

  • 59

    Sperm Motility Normal:

    MOTILITY OF 50% OF THE SPERM WITH RATING OF 2

  • 60

    Sperm Motility ? movement in a well-mixed liquefied specimen

    Forward, progressive

  • 61

    Sperm Motility Count in ? AND evaluate ?

    20 HPF ; 200 sperm/slide

  • 62

    w/n 1 hr, 2 50% of sperms should be motile in

    CATEGORIES A, B , C

  • 63

    >/ = 25% should show Progressive Motility in

    A & B

  • 64

    Sperm Morphology SHAPED

    OVAL SHAPED

  • 65

    Sperm Morphology

    5 um long & 3 um wide and a long flagella (45 um)

  • 66

    approximately half and cover 2/3 of sperm nucleus for ovum penetration

    head

  • 67

    attaches the head to tail

    NECK PIECE

  • 68

    thickest; surrounded by mitochondrial sheath that produces energy required for energy

    MID PIECE

  • 69

    SPERM STAIN

    Wright, Giemsa, Papanicolaou stain

  • 70

    stain of choice and doesnt require heat fixation

    Papanicolaou

  • 71

    MORPHOLOGY (Kruger's strict):

    >14% normal

  • 72

    Normal: Normal morphology

    >14%

  • 73

    Routine: Normal morphology

    >30%

  • 74

    abnormal Morphology

    >70%

  • 75

    differentiate spermatids (-) & leukocytes (+)

    peroxidase

  • 76

    Sperm Vitality/aka

    BLOOM'S TEST

  • 77

    Red against a purple background

    DEAD CELLS

  • 78

    Are not infiltrated by the stain bluish white

    LIVING CELLS

  • 79

    LIVING CELLS Are not infiltrated by the stain ?

    bluish white

  • 80

    Dead Cells:

    Red against a purple background

  • 81

    Normal: ___living cells: VITALITY

    >50%

  • 82

    defective flagellum

    Vital but immobile:

  • 83

    Immotile & non-viable cells:

    Epididymal pathology

  • 84

    Seminal Fluid Fructose TEST

    Resorcinol Test:

  • 85

    Resorcinol Test:

    ORANGE/ ORANGE RED

  • 86

    Test for choline Rgts: K iodide, iodine crystals (+) BROWN RHOMBIC CRYSTALS

    FLORENCE TEST

  • 87

    FLORENCE TEST REAGENTS

    K iodide, iodide crystals

  • 88

    Test for spermin Rgt: Picric Acid, TCA yellow leaf like structure

    barbieros test

  • 89

    barbieros test structure

    yellow leaf like

  • 90

    barbieros test is for? what reagents?

    spermin rgts: Picric Acid, TCA

  • 91

    Test for tenacity of mucus

    SPINBARKITT TEST

  • 92

    SPINBARKITT TEST

    Test for tenacity of mucus

  • 93

    Test for ability of sperm cells to penetrate the cervical mucosa, Post-coital test

    SIMS HUHNER TEST

  • 94

    More specific: IgG, IgM, IgA & demonstrate what area of sperm does the ab attached Polyacrylamide beads coated w/ anti-IgG, IgM, IgA, IgM tail ab, IgG head

    Immunobead Test

  • 95

    Immunobead Test NORMAL:

    <50% OF SPERM

  • 96

    ROUND CELLS: Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum

    >1 MILLION WBC/ML

  • 97

    RAPE CASES

    SPECIMEN+XYLENE

  • 98

    ACID PHOSPHATASES, SEMINAL GLYCOPROTEIN P3D

    RAPE CASES

  • 99

    VISCOSITY:

    POURS IN DROPLETS

  • 100

    ROUND CELLS: DISEASES

    Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum

    • other names parasitology

    other names parasitology

    Yves Laure Pimentel · 70問 · 2年前

    other names parasitology

    other names parasitology

    70問 • 2年前
    Yves Laure Pimentel

    PARASITOLOGY

    PARASITOLOGY

    Yves Laure Pimentel · 111問 · 2年前

    PARASITOLOGY

    PARASITOLOGY

    111問 • 2年前
    Yves Laure Pimentel

    HTMLBE

    HTMLBE

    Yves Laure Pimentel · 64問 · 2年前

    HTMLBE

    HTMLBE

    64問 • 2年前
    Yves Laure Pimentel

    CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

    CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

    Yves Laure Pimentel · 61問 · 2年前

    CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

    CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

    61問 • 2年前
    Yves Laure Pimentel

    HISTOPATHOLOGY

    HISTOPATHOLOGY

    Yves Laure Pimentel · 69問 · 2年前

    HISTOPATHOLOGY

    HISTOPATHOLOGY

    69問 • 2年前
    Yves Laure Pimentel

    CC-2 SPECTROPHOTOMETRY

    CC-2 SPECTROPHOTOMETRY

    Yves Laure Pimentel · 100問 · 2年前

    CC-2 SPECTROPHOTOMETRY

    CC-2 SPECTROPHOTOMETRY

    100問 • 2年前
    Yves Laure Pimentel

    CC-3 ELECTROPHORESIS

    CC-3 ELECTROPHORESIS

    Yves Laure Pimentel · 41問 · 2年前

    CC-3 ELECTROPHORESIS

    CC-3 ELECTROPHORESIS

    41問 • 2年前
    Yves Laure Pimentel

    AUBF LAB SAFETY

    AUBF LAB SAFETY

    Yves Laure Pimentel · 87問 · 2年前

    AUBF LAB SAFETY

    AUBF LAB SAFETY

    87問 • 2年前
    Yves Laure Pimentel

    CSF 1

    CSF 1

    Yves Laure Pimentel · 100問 · 2年前

    CSF 1

    CSF 1

    100問 • 2年前
    Yves Laure Pimentel

    CSF 2

    CSF 2

    Yves Laure Pimentel · 82問 · 2年前

    CSF 2

    CSF 2

    82問 • 2年前
    Yves Laure Pimentel

    SEMEN 2

    SEMEN 2

    Yves Laure Pimentel · 7問 · 2年前

    SEMEN 2

    SEMEN 2

    7問 • 2年前
    Yves Laure Pimentel

    SYNOVIAL FLUID 1

    SYNOVIAL FLUID 1

    Yves Laure Pimentel · 100問 · 2年前

    SYNOVIAL FLUID 1

    SYNOVIAL FLUID 1

    100問 • 2年前
    Yves Laure Pimentel

    SYNOVIAL FLUID 2

    SYNOVIAL FLUID 2

    Yves Laure Pimentel · 6問 · 2年前

    SYNOVIAL FLUID 2

    SYNOVIAL FLUID 2

    6問 • 2年前
    Yves Laure Pimentel

    SEROUS FLUID

    SEROUS FLUID

    Yves Laure Pimentel · 25問 · 2年前

    SEROUS FLUID

    SEROUS FLUID

    25問 • 2年前
    Yves Laure Pimentel

    PLEURAL FLUID

    PLEURAL FLUID

    Yves Laure Pimentel · 44問 · 2年前

    PLEURAL FLUID

    PLEURAL FLUID

    44問 • 2年前
    Yves Laure Pimentel

    PERICARDIAL FLUID

    PERICARDIAL FLUID

    Yves Laure Pimentel · 18問 · 2年前

    PERICARDIAL FLUID

    PERICARDIAL FLUID

    18問 • 2年前
    Yves Laure Pimentel

    PERITONEAL FLUID/ ASCITIC FLUID

    PERITONEAL FLUID/ ASCITIC FLUID

    Yves Laure Pimentel · 30問 · 2年前

    PERITONEAL FLUID/ ASCITIC FLUID

    PERITONEAL FLUID/ ASCITIC FLUID

    30問 • 2年前
    Yves Laure Pimentel

    AMNIOTIC FLUID

    AMNIOTIC FLUID

    Yves Laure Pimentel · 92問 · 2年前

    AMNIOTIC FLUID

    AMNIOTIC FLUID

    92問 • 2年前
    Yves Laure Pimentel

    FECALYSIS

    FECALYSIS

    Yves Laure Pimentel · 80問 · 2年前

    FECALYSIS

    FECALYSIS

    80問 • 2年前
    Yves Laure Pimentel

    問題一覧

  • 1

    Paired glands inside the scrotum and contain semineferous glands

    TESTES

  • 2

    site of spermatogenesis

    semineferous glands

  • 3

    production of spermatozoa and located in epithelial cells of seminiferous tubules

    GERM CELLS

  • 4

    provide support & nutrients for the germ cells as they undergo spermatogenesis => complete > Immature sperm enter EPIDIDYMIS where sperm mature & develops flagella & stored until ejaculation

    SORTOLI CCELLS

  • 5

    propels sperm to ejaculatory duct w/ the fluid from seminal vesicles

    VAS DEFERENS

  • 6

    produce most fluid in semen (60-70%)- transport fluid of sperm

    SEMINAL VESICLE

  • 7

    produce most fluid in semen ____- transport fluid of sperm

    (60-70%)

  • 8

    Energy for flagella)

    FRUCTOSE

  • 9

    (Gray appearance of the semen)

    FLAVIN

  • 10

    produces 20-30% of semen volume (acidic)

    PROSTATE GLAND

  • 11

    produces ___ of semen volume (acidic)

    20-30%

  • 12

    Differs from other fluids

    ACID PHOSPHATASE

  • 13

    DECREASE amount: Disorders of prostate

    ZINC

  • 14

    controls liquefaction & coagulation of seminal fluid

    PROTEOLYTIC ENZYMES

  • 15

    antibacterial

    SPERMINE AND CHOLINE

  • 16

    produces 5% of the fluid volume composed of thick..alkaline mucus

    BULBOURETHRAL GLANDS

  • 17

    BULBOURETHRAL GLANDS produces how many % of the fluid volume composed of thick..alkaline mucus

    5%

  • 18

    Neutralize the acidity from the prostatic secretion & vagina

    ALKALINE MUCUS

  • 19

    ALKALINE MUCUS: Neutralize the acidity from the prostatic secretion & vagina w/o this neutralization:

    Motility is diminished

  • 20

    Sexual abstinence:

    Atleast 2 days but not > 7 days

  • 21

    WHO fertility testing: ? samples (? intervals)

    2-3; 2-weekS

  • 22

    liquefaction:

    30 min.-1 hr

  • 23

    best Method of Collection:

    MASTURBATION

  • 24

    Method of Collection Not reliable = 1# portion of ejaculate is loss 2 | sperm count; low pH: affects motility

    Coitus Interruptus/ Withdrawal method

  • 25

    aspiration of semen from vaginal vault after coitus

    Vaginal Vault Aspiration

  • 26

    Non-lubricant rubber/ Poly-urethane condoms; Ordinary condom: spermatocides

    Condom Method:

  • 27

    ALL SPX ARE POTENTIAL SOURCE OF

    HIV/ HBV

  • 28

    Preservation o @?: Transport

    RT

  • 29

    Body temp (?): While awaiting analysis

    37C

  • 30

    Fructose level: tested w/n ? For frozen fo prevent FRUCTOLYSIS -85 DEGREES CELCIUS

    2 HOURS

  • 31

    Fructose level: tested w/n 2 HOURS For frozen fo prevent ?

    FRUCTOLYSIS -85 DEGREES CELCIUS

  • 32

    SEMEN ANALYSIS = Appearance o Normal Color:

    GRAY-WHITE (Translucent/ Pearly-white)

  • 33

    SEMEN Odor:

    Musty odor, Fishy, BLEACH LIKE/ CLOROX LIKE

  • 34

    Clear specimen:

    DECREASED sperm conc.: infertility

  • 35

    COLOR: Urine contamination, antibiotics, prolonged abstinence, pyospermia

    Yellow

  • 36

    If after 2 hrs, the specimen not liquefied: add:

    DULBECCOS PHOSPHATE BUFFERED SALINE Proteolytic enzymes: A- CHYMOTRYPSIN & BROMELAIN

  • 37

    : Liquefied specimen

    Jelly-like granules (gelatinous bodies)

  • 38

    Volume o Normal: semen

    2-5 ml

  • 39

    Refers to the consistency of the fluid

    Viscosity

  • 40

    Incomplete liquefied specimen:

    clumped & highly viscous

  • 41

    Droplets that forms thread longer than 2 cm:

    ABNORMAL/ HIGLY VISCOUS

  • 42

    Viscosity IS 0

    WATERY

  • 43

    Viscosity IS 4

    GEL LIKE

  • 44

    Indicates the balance bet. from the acidic prostatic secretion & alkaline seminal vesicles secretion

    pH

  • 45

    Normal pH:

    7.2 - 8.0

  • 46

    increased pH:

    Reproductive tract infection

  • 47

    decreased pH:

    increased prostatic fluid, ejaculatory duct obstruction, poorly developed seminal vesicles

  • 48

    Normal Sperm Concentration:

    20-250 million/ mL

  • 49

    Sperm Concentration & Sperm Count Borderline:

    10-20 million/ mL

  • 50

    Sperm Concentration & Sperm Count USING

    NEUBAUER COUNTING CHAMBER

  • 51

    diluting fluids: (both immobilize & preserve cells)

    SODIUM BICARBONATE, FORMALIN

  • 52

    can also be used as Diluting fluids:

    SALINE, distilled H20 (cold) or Tap H20

  • 53

    inflammation/ infection w/n reproductive tract

    1 million leukocyte/ml:

  • 54

    Spermatogenesis disruption, viral infections, toxic chemicals

    1 million spermatids/ml

  • 55

    undiluted spx, sperms are immobilized by heating, sperm motility is assessed unheated portion

    MAKLER

  • 56

    Complete/ total/Absence of spermatozoa seen

    AZOOSPERMIA

  • 57

    presence of sperm cells whether completely dead or immobile

    NECROSPERMIA

  • 58

    Deficiency in the number of sperm cells or presence of few motile cells seen

    OLIGOSPERMIA

  • 59

    Sperm Motility Normal:

    MOTILITY OF 50% OF THE SPERM WITH RATING OF 2

  • 60

    Sperm Motility ? movement in a well-mixed liquefied specimen

    Forward, progressive

  • 61

    Sperm Motility Count in ? AND evaluate ?

    20 HPF ; 200 sperm/slide

  • 62

    w/n 1 hr, 2 50% of sperms should be motile in

    CATEGORIES A, B , C

  • 63

    >/ = 25% should show Progressive Motility in

    A & B

  • 64

    Sperm Morphology SHAPED

    OVAL SHAPED

  • 65

    Sperm Morphology

    5 um long & 3 um wide and a long flagella (45 um)

  • 66

    approximately half and cover 2/3 of sperm nucleus for ovum penetration

    head

  • 67

    attaches the head to tail

    NECK PIECE

  • 68

    thickest; surrounded by mitochondrial sheath that produces energy required for energy

    MID PIECE

  • 69

    SPERM STAIN

    Wright, Giemsa, Papanicolaou stain

  • 70

    stain of choice and doesnt require heat fixation

    Papanicolaou

  • 71

    MORPHOLOGY (Kruger's strict):

    >14% normal

  • 72

    Normal: Normal morphology

    >14%

  • 73

    Routine: Normal morphology

    >30%

  • 74

    abnormal Morphology

    >70%

  • 75

    differentiate spermatids (-) & leukocytes (+)

    peroxidase

  • 76

    Sperm Vitality/aka

    BLOOM'S TEST

  • 77

    Red against a purple background

    DEAD CELLS

  • 78

    Are not infiltrated by the stain bluish white

    LIVING CELLS

  • 79

    LIVING CELLS Are not infiltrated by the stain ?

    bluish white

  • 80

    Dead Cells:

    Red against a purple background

  • 81

    Normal: ___living cells: VITALITY

    >50%

  • 82

    defective flagellum

    Vital but immobile:

  • 83

    Immotile & non-viable cells:

    Epididymal pathology

  • 84

    Seminal Fluid Fructose TEST

    Resorcinol Test:

  • 85

    Resorcinol Test:

    ORANGE/ ORANGE RED

  • 86

    Test for choline Rgts: K iodide, iodine crystals (+) BROWN RHOMBIC CRYSTALS

    FLORENCE TEST

  • 87

    FLORENCE TEST REAGENTS

    K iodide, iodide crystals

  • 88

    Test for spermin Rgt: Picric Acid, TCA yellow leaf like structure

    barbieros test

  • 89

    barbieros test structure

    yellow leaf like

  • 90

    barbieros test is for? what reagents?

    spermin rgts: Picric Acid, TCA

  • 91

    Test for tenacity of mucus

    SPINBARKITT TEST

  • 92

    SPINBARKITT TEST

    Test for tenacity of mucus

  • 93

    Test for ability of sperm cells to penetrate the cervical mucosa, Post-coital test

    SIMS HUHNER TEST

  • 94

    More specific: IgG, IgM, IgA & demonstrate what area of sperm does the ab attached Polyacrylamide beads coated w/ anti-IgG, IgM, IgA, IgM tail ab, IgG head

    Immunobead Test

  • 95

    Immunobead Test NORMAL:

    <50% OF SPERM

  • 96

    ROUND CELLS: Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum

    >1 MILLION WBC/ML

  • 97

    RAPE CASES

    SPECIMEN+XYLENE

  • 98

    ACID PHOSPHATASES, SEMINAL GLYCOPROTEIN P3D

    RAPE CASES

  • 99

    VISCOSITY:

    POURS IN DROPLETS

  • 100

    ROUND CELLS: DISEASES

    Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum