CC-3 ELECTROPHORESIS

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

- a molecule, such as protein, whose net charge can be either positive or negative

Ampholyte

– pH where protein has no net charge

Isoelectric point (pI)

Particle with No net charge won’t migrate, MIGRATION of Charged particles

Electrophoresis

At pH 8.6 proteins migrate and divide to:

Albumin, a1 globulin, a2 globulin, beta globulin, and gamma globulin

At what pH proteins migrate and divide to: Albumin, a1 globulin, a2 globulin, beta globulin, and gamma globulin

8.6

- migration of small ions

Iontophoresis

- migration of charged macromolecules (protein)

Zone electrophoresis

separation is performed in narrow-bore fused silica capillaries

Capillary Electrophoresis

MIGRATION of Charged particles

Electrophoresis

Capillary Electrophoresis molecules are separated by?

electro-osmotic flow (EOF)

molecules are separated by electro-osmotic flow (EOF)

Capillary Electrophoresis

Capillary Electrophoresis - charge w/c moves faster

Positive (+)

Capillary Electrophoresis - charge w/c moves slower

Negative (-)

it separates according to charge and not size

Isoelectric Focusing

Isoelectric Focusing i. molecules migrate thru a pH ________.

gradient

molecules migrate thru a pH gradient.

Isoelectric Focusing

Isoelectric Focusing Ideal for separating proteins of IDENTICAL _____ but w/DIFFERENT NET CHARGES

size

Ideal for separating proteins of IDENTICAL size but w/DIFFERENT NET CHARGES

Isoelectric Focusing

- separate proteins into as many as 12 zones

High-resolution protein electrophoresis

High-resolution protein electrophoresis - separate proteins into as many as ? zones

COMPONENTS OF ELECTROPHORESIS driving force is?

ELECTRICITY

COMPONENTS OF ELECTROPHORESIS separates by charge and molecular size

Starch gel

COMPONENTS OF ELECTROPHORESIS separates by molecular size, used in Isoelectric focusing

Cellulose acetate

Agarose gel – _______, does not bind to protein and separates by electrical charge

NEUTRAL

COMPONENTS OF ELECTROPHORESI – NEUTRAL, does not bind to protein and separates by electrical charge

Agarose gel

COMPONENTS OF ELECTROPHORESI – neutral, separates by charge and size, separates protein into 20 fractions. For ISOENZYMES

Polyacrylamide gel

COMPONENTS OF ELECTROPHORESI - monovalent ions

buffer

– measures absorbance of stain

Densitometry

Densitometry- Scan and _____________ electrophoretic pattern

QUANTITATE

- Scan and QUANTITATE electrophoretic pattern

Densitometry

Detection and Quantitation (2)

Densitometry UV

Specimen Treatment Serum - High protein concentration = must be ________

DILUTED

Specimen Treatment - High protein concentration = must be DILUTED

Serum

Specimen Treatment . Urine and CSF - low protein = _____________

CONCENTRATED

Specimen Treatment . - low protein = CONCENTRATED

Urine and CSF

Specimen Treatment - no concentration

Hemoglobin hemolysate

is used for CSF proteins

Silver nitrate

fat red 7B and oil red O are used_________________,

LIPOPROTEIN

are used LIPOPROTEIN,

fat red 7B and oil red O

is used for lactate dehydrogenase isoenzymes

nitrotetrazolium blue

nitrotetrazolium blue is used for

lactate dehydrogenase isoenzymes

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