– enhance phagocytosis

- internalized surface membrane

– contains cytoplasmic granules needed for digestion.

. – fusion of Phagosome and lysosome

– deficient NADPH oxidase - >SEVERE RECURRENT INFECTION

defect in oxygen-dependent peroxidation pathway/Cytochrome B defect

most common and best characterized NEUTROPHIL abnormality

catalase-positive bacteria and fungi

MOST IMPORTANT AGENT necessary for producing the toxic superoxides that are critical in bacterial killing.

Nitroblue Tetrazolium Reduction Test (NBT) Yellow/Colorless (?)

Nitroblue Tetrazolium Reduction Test (NBT) ?(tetrazolium)

Nitroblue Tetrazolium Reduction Test (NBT) Deep blue color/brownish black precipitate (?)

Nitroblue Tetrazolium Reduction Test (NBT) (formazan)

Yellow/colorless (tetrazolium) results in

Deep blue color/brownish black precipitate (formazan) RESULTS

CONFIRMATORY OF CGD

– most important mechanism of BACTERIAL KILLING

- primary granules of neutrophils is DECREASED/ ABSENT

- DECREASED in the amount of hydrogen peroxide produced

- abnormal fusion of primary granules in neutrophils

assesses phagocytic activity