18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

Most frequently used: ? (both QUANTITATIVE)

Radial immunodiffusion (RID) and Nephelometry

ASSAYS FOR CLASSICAL PATHWAY; Functional Tests

Hemolytic Titration (CH50) ELISA

most commonly used FOR CLASSICAL PATHWAY

Hemolytic Titration (CH50)

measures the amount of ptx serum required to lyse 50% of a standardized concentration of Absensitized Sheep RBC

Hemolytic Titration (CH50)

Hemolytic Titration (CH50) measures the amount of ptx serum required to lyse ?% of a standardized concentration of Absensitized Sheep RBC

50%

based on lysis of liposomes that release an enzyme when lysed. More accurate than no.1

CH50 TEST

– Best screening for complement abnormalities.

ELISA

Can also detect complement activation split prod.

ELISA

used to detect the presence of specific Ag or Ab.

COMPLEMENT FIXATION TEST

Application -> detect Ab to virus, rickettsia, and fungi

COMPLEMENT FIXATION TEST

COMPLEMENT FIXATION TEST Application -> detect Ab to ?

virus, rickettsia, and fungi

COMPLEMENT FIXATION TEST Two Stage Process

Test system Indicator system

which lyse in the presence of complement

Indicator system

COMPLEMENT FIXATION POSITIVE =

NO HEMOLYSIS

COMPLEMENT FIXATION = NO HEMOLYSIS

POSITIVE

COMPLEMENT FIXATION NEGATIVE =

HEMOLYSIS

COMPLEMENT FIXATION = HEMOLYSIS

NEGATIVE

? must be heated to 56C for 30 min. to destroy complement

Serum

Serum must be heated to ? to destroy complement

56C for 30 min.

other names parasitology

other names parasitology

70問 • 2年前

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PARASITOLOGY

PARASITOLOGY

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HTMLBE

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CC - 1 LAB. SAFETY, BASIC UNITS, AND CONVERSION FACTOR

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HISTOPATHOLOGY

HISTOPATHOLOGY

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CC-2 SPECTROPHOTOMETRY

CC-2 SPECTROPHOTOMETRY

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CC-3 ELECTROPHORESIS

CC-3 ELECTROPHORESIS

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AUBF LAB SAFETY

AUBF LAB SAFETY

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CSF 1

CSF 1

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CSF 2

CSF 2

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SEMEN 1

SEMEN 1

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SEMEN 2

SEMEN 2

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SYNOVIAL FLUID 1

SYNOVIAL FLUID 1

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SYNOVIAL FLUID 2

SYNOVIAL FLUID 2

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SEROUS FLUID

SEROUS FLUID

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PLEURAL FLUID

PLEURAL FLUID

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PERICARDIAL FLUID

PERICARDIAL FLUID

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PERITONEAL FLUID/ ASCITIC FLUID

PERITONEAL FLUID/ ASCITIC FLUID

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AMNIOTIC FLUID

AMNIOTIC FLUID

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FECALYSIS

FECALYSIS

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