10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

Yves Laure Pimentel · 70問 · 2年前

通報

問題一覧

SEPARATION of Mononuclear cells from the WB and detection of Surface markers

FICOLL HYPAQUE

FICOLL HYPAQUE SG:

1.077 AND 1.114

FICOLL HYPAQUE Sample:

Diluted defribinated blood or/heparin

WB + FH -> Centrifuged -> ? RPM/30minutes -> four layers

400g

WB + FH -> Centrifuged -> 400g RPM/time? -> four layers

30minutes

WB + FH -> Centrifuged -> 400g RPM/30minutes -> ? layers

four

Surface Ag tested in T Cell are

CD2 CD3 CD4 CD7 CD8

Surface Ag tested B Cell are

CD19 CD20 CD22

Manual Method for ID:

Rosette Technique

Traditionally used

Rosette Technique

Rosette Technique Specimen

Whole blood

Rosette Technique Reagent

sheep rbc

Rosette Technique Surface Ag -________ found only on T cells

CD2

Rosette Technique Result -

Rosette formation

MITOGENIC SUBSTANCE THAT STIMULATE T CELL BLASTIC TRANSFORMATION

1. Concanavalin A (ConA) 2. Phytohemagglutinin A (PHA) – 3. Pokeweed mitogen (PWM)

MITOGENIC SUBSTANCE THAT STIMULATE B CELL BLASTIC TRANSFORMATION

1. Lipopolysaccharides 2. Protein A 3. PHA 4. PWM 5. Phorbol myristate 6. Anti-IgM antibody

other names parasitology

other names parasitology

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