MICROBIOLOGY & PARASITOLOGY PROGRESS EXAM PART 2

32問 • 1年前

問題一覧

When 10% CYANOGEN BROMIDE and 4% aniline in 96% ethanol are added to a suspension of the culture, a CANARY YELLOW color indicates a positive reaction: * 1/1 Arylsulfatase test Heat-stable catalase test Niacin test Nitrate reduction test

Niacin test

What two reagents must be added to determine if the bacterium is VP positive? * 1/1 Creatine and 1N HC1 10% FeCl3 and alpha-naphthol Kovac's reagent and zinc dust Alpha-naphthol and 40% KOH

Alpha-naphthol and 40% KOH

Which of the following is a key characteristic of Coccidioides immitis? * 1/1 Has a higher dissemination rate in white females Is endemic in the northeastern United States Produces endosporulating spherules in tissue Forms foot cells

Produces endosporulating spherules in tissue

Which of the following is a key characteristic by which an unknown Cryptococcus spp. can be identified as Cryptococcus neoformans? * 0/1 Appearance of yellow colonies b. Positive urease test Positive urease test Presence of a capsule Positive niger seed agar test

Positive niger seed agar test

The protein coat that surrounds the nucleic acid of a virion is called which of the following? * 1/1 Capsomere Capsid Capsule Nucleocapsid

Capsid

The test of choice and most sensitive assay for use with CSF to diagnose aseptic meningitis caused by enterovirus is which of the following? * 1/1 Cell culture PCR Antigenemia immunoassay Shell vial assay

PCR

Which preservation method is most suitable and the most widely used for subsequent fixed smear preparation? * 0/1 Formalin-ethyl acetate PVA Trichrome MIF

PVA

The only known human tapeworm with an operculum is: * 1/1 Diphyllobothrium latum Hymenolepis nana Giardia lamblia Schistosoma haematobium

Diphyllobothrium latum

To quantify malaria parasites against RBCs Count the parasitized RBCs among 500-2,000 RBCs on the thin smear and express the results as: * 0/1 % Parasitemia Parasites per microliter of blood

% Parasitemia

To quantify malaria parasites against WBCs On the thick smear, tally the parasites against WBCs, until you have counted 500 parasites or 1,000 WBCs, whichever comes first; express the results as: * 0/1 % Parasitemia Parasites per microliter of blood

Parasites per microliter of blood

In disk diffusion susceptibility testing, as an antimicrobial agent diffuses away from the disk, the concentration of antibiotic is: * 1/1 Increased Decreased Unchanged Inoculum dependent

Decreased

Mycobacterium tuberculosis complex can be identified directly in AFB smear positive respiratory specimens the same day the smear is read by: * 0/1 Cording seen on the AFB smear b molecular testing Molecular testing QuantiFERON®-TB test MALDI-TOF testing

Molecular testing

Which testing platform meets this description? An isolated colony is irradiated by a laser, which ionizes the biomolecules and causes them to become accelerated in an electric field. The ionized biomolecules then enter a flight tube where they are separated by their mass-to-charge ratio. * 0/1 MALDI-TOF Multiplex PCR Pulsed-field gel electrophoresis Sequencing

MALDI-TOF

PCR involving the sequential use of two primer sets: * 0/1 Multiplex PCR Nested PCR Arbitrary primed PCR RT-PCR

Nested PCR

How can PCR be applied to the detection of human immunodeficiency and other RNA viruses? * 0/1 The virus must be inserted into human DNA by viral integrase prior to PCR Substitute deoxyuridine triphosphate in place of deoxythymidine triphosphate in the master mix Add a heat-stable reverse transcriptase enzyme to the master mix Substitute ribonucleotide triphosphates for deoxyribonucleotide triphosphates in the master mix

Add a heat-stable reverse transcriptase enzyme to the master mix

ISOTHERMAL AMPLIFICATION Loop-mediated isothermal amplification (LAMP) uses _____ primers and proceeds using a constant temperature coupled to a strand displacement reaction. * 0/1 2 primers 3 primers 4 primers 5 primers

4 primers

Actinomyces: * 1/1 Gram positive facultative anaerobe Gram negative facultative anaerobe Gram positive cocci Gram negative cocci

Gram positive facultative anaerobe

The biochemical tests performed on a gram-positive bacillus were consistent with those of Corynebacterium diphtheriae. The MLS should now: * 0/1 Perform a spore stain of the colonies Determine if the isolate is toxigenic by performing an Elek test Perform an agglutination test to confirm the organism's identity Subculture the organism to Hektoen enteric medium and examine for black colonies

Determine if the isolate is toxigenic by performing an Elek test

Production of exotoxin A, which kills host cells by inhibiting protein synthesis and production of several proteolytic enzymes and hemolysins that destroy cells and tissue are factors that contribute to pathogenicity of which of the following organisms? * 1/1 Pseudomonas aeruginosa Burkholderia cepacia Ralstonia pickettii Burkholderia mallei

Pseudomonas aeruginosa

An anaerobic, box-shaped gram-positive bacillus is positive for the reverse CAMP test. This organism would also be: * 1/1 Lipase positive Indole positive Lecithinase positive Spore negative

Lecithinase positive

During a blood meal, a malaria-infected female Anopheles mosquito INOCULATES ______into the human host. * 1/1 Gametocytes Hypnozoites Merozoites Sporozoites

Sporozoites

Ingested by an Anopheles mosquito during a blood meal: * 1/1 Gametocytes Hypnozoites Merozoites Sporozoites

Gametocytes

First-stage larva of flukes that emerge from the egg in fresh water, equipped with cilia, which aid in movement: Cercaria Metacercaria Redia Miracidium

Miracidium

Identify: Cercaria Metacercaria Redia Miracidium

Miracidium

Biological safety hood * Class I Class IIA Class IIB Class III

Class I

Biological safety hood * Class I Class IIA Class IIB Class III

Class IIA

Biological safety hood * Class I Class IIA Class IIB Class III

Class IIB

Biological safety hood * Class I Class IIA Class IIB Class III

Class III

Similar to hookworm eggs: * 1/1 Ascaris Trichuris Trichostrongylus Enterobius

Trichostrongylus

Mystery disease: * 0/1 Ascariasis Filariasis Capillariasis Taeniasis

Capillariasis

Eggs can be confused with POLLEN GRAINS: * 1/1 Diphyllobothrium latum Dipylidium caninum Hymenolepis nana Taenia spp.

Taenia spp.

Gravid proglottids resemble RICE GRAINS (dry) or CUCUMBER SEEDS (moist): * 1/1 Diphyllobothrium latum Dipylidium caninum Hymenolepis nana Taenia spp.

Dipylidium caninum

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Yves Laure Pimentel · 64問 · 2年前

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