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MICROBIOLOGY BOC

MICROBIOLOGY BOC
100問 • 1年前
  • Yves Laure Pimentel
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    問題一覧

  • 1

    Crust from a cauliflower-like lesion on the hand exhibited brown spherical bodies 6-12 um in diameter when examined microscopically. After 3 weeks of incubation at room temperature, a slow-growing black mold grew on Sabouraud dextrose agar. Microscopic examination revealed Cladosporium, Phialophora and Fonsecaea types of sporulation. The probable identification of this organism is: a Fonsecaea pedrosoi b Pseudallescheria boydii c Phialephora verrucosa d Cladosporium carrionii

    a Fonsecaea pedrosoi

  • 2

    Pus from a draining fistula on a foot was submitted for culture. Gross examination of the specimen revealed the presence of a small (0.8 mm in diameter), yellowish, oval granule. Direct microscopic examination of the crushed granule showed hyphae 3-4 um in diameter and the presence of chlamydospores at the periphery. After 2 days a cottony, white mold was seen that turned gray with a gray to black reverse after a few days. When viewed microscopically, moderately large hyaline septate hyphae with long or short conidiophores, each with a single pear-shaped conidium, 5-7 x 8-10 pm, were seen. The most likely identification is: a_ Exophiala jeanselmei b Fonsecaea pedrosoi c Pseudallescheria boydii d Cladosporium carrionii

    c Pseudallescheria boydii

  • 3

    In processing clinical specimens and fungal isolates, laboratory workers may contract systemic fungal infections through: a inhalation b ingestion c skin contact d insect vector

    a inhalation

  • 4

    A sputum specimen received at 8 AM for an AFB smear reveals acid- fast bacilli. An additional sputum is submitted that afternoon. This specimen was concentrated by the NALC-sodium hydroxide method and inoculated on 2 Lowenstein-Jensen slants and held for 8 weeks at 35°C in 5%-10% CO», No growth occurs. ‘Ihe best explanation is that: the hypochlorite technique was not used improper specimen was submitted for culture improper media was used for culture cultures were held for an insufficient period of time

    improper specimen was submitted for culture

  • 5

    ‘The preferred carbon source for mycobacteria is: a_ glycerol b glucose c fatty acids d casein hydrolysate

    a_ glycerol

  • 6

    first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Lowenstein-Jensen slants are incubated in the dark at 35°C with 5%-10% CO,. The smears reveal acid-fast bacilli, and after 7 days no growth appears on the slants. The best explanation is: a improper specimen submitted b incorrect concentration procedure c exposure to CO, prevents growth d cultures held for insufficient length of time

    d cultures held for insufficient length of time

  • 7

    A first morning sputum specimen is received for acid-fast culture. The specimen is centrifuged, and the sediment is inoculated on 2 Lowenstein-Jensen slants, which are incubated at 35°C in 5%-10% CO. After 1 week, the slants show abundant growth over the entire surface. Stains reveal gram-negative bacilli. To avoid this problem: a utilize a medium that inhibits bacterial growth b add sodium hypochlorite to the sediment before inoculation c incubate the tubes at room temperature to retard bacterial growth d decontaminate the specimen with sodium hydroxide

    d decontaminate the specimen with sodium hydroxide

  • 8

    A first morning sputum is received for culture of acid-fast bacilli. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Sabouraud dextrose slants are incubated in the dark at 35°C with 5%-10% COy. The smears reveal acid-fast bacilli, but the slants show no growth after 8 weeks. The explanation is: a improper media used b incorrect concentration procedure used c improper specimen submitted d exposure to CO, prevents growth

    a improper media used

  • 9

    In reviewing the number of Mycobacterium isolates for the current year, it was noted that there were 76% fewer isolates than the previous year (115 vs 28). The technologist in charge of the area has documented that the quality control of media, reagents and stains has been acceptable and there has been no gross contamination of the cultures noted. The most appropriate course of action to pursue would be: a stop use of commercial media and produce in-house b change to different formulations of egg and agar based media c change over to the Bactec™ system for isolation of Mycobacterium d review the digestion and decontamination procedure

    an review the digestion and decontamination procedure

  • 10

    Which of the following combinations of media provides an egg base, agar base, and a selective egg or agar base media? a Lowenstein-Jensen, American Thoracic Society (ATS), Middlebrook 7H11 b Lowenstein-Jensen, Middlebrook 7H11, Lowenstein-Jensen Gruft c Middlebrook 7H10, Petragnani, Lowenstein-Jensen [-7 Middlebrook 7H10, Middlebrook 7H11, Mitchison 7H11

    b Lowenstein-Jensen, Middlebrook 7H11, Lowenstein-Jensen Gruft

  • 11

    Which of the following reagents should be used as a mucolytic, alkaline reagent for digestion and decontamination of a sputum for mycobacterial culture? a N-acetyl-L-cystine and NaOH b NaOH alone c zephiran-trisodium phosphate d_ oxalic acid

    a N-acetyl-L-cystine and NaOH

  • 12

    The function of N-acetyl-L-cysteine in the reagent for acid-fast digestion-decontamination procedure is to: a inhibit growth of normal respiratory flora b inhibit growth of fungi c neutralize the sodium hydroxide d_ liquefy the mucus

    d liquefy the mucus

  • 13

    When staining acid-fast bacilli with Truant auramine-rhodamine stain, potassium permanganate is used as a: a decolorizing agent b quenching agent c mordant d dye

    b quenching agent

  • 14

    Middlebrook 7H10 and 7H11 media must be refrigerated in the dark, and incubated in the dark as well. If these conditions are not met, the media may prove toxic for mycobacteria because: a_ carbon dioxide will be released b growth factors will be broken down c light destroys the ammonium sulfate d formaldehyde may be produced

    d formaldehyde may be produced

  • 15

    The method used for processing specimens for mycobacterial culture contaminated with Pseudomonas is: a N-acetyl-L-cystine and NaOH b NaOH ¢ zephiran-trisodium phosphate d_ oxalic acid

    d_ oxalic acid

  • 16

    An AEB broth culture is positive for acid-fast bacilli at 1 week while the agar slant shows no growth. The most likely explanation for this is: a_ the organism is a contaminant b AFB grow more rapidly in liquid media ¢ PANTA was added to the broth d_ the agar slant was incubated in 5% CO,

    b AFB grow more rapidly in liquid media

  • 17

    A bronchial washing is processed for acid-fast bacilli. Which of the following precautions should be taken in order to prevent infection of laboratory personnel? add an equal amount of NALC to the specimen process all specimens under ultraviolet light centrifuge specimen only after the addition of preservative process all specimens in a biological safety hood

    process all specimens in a biological safety hood

  • 18

    Tubercle bacilli are specifically stained by: crystal violet 1% acid fuchsin methylene blue carbol fuchsin

    carbol fuchsin

  • 19

    ‘The species of mycobacteria that will give a positive niacin test is Mycobacterium: a leprae b kansasii c fortuitum d_ tuberculosis

    d_ tuberculosis

  • 20

    A digested and decontaminated sputum is inoculated into a Bactec™ 12B bottle and incubated in air at 37°C, On day 14, a positive growth index is obtained, and the auramine-rhodamine stain is positive. Broth from the initial bottle is inoculated into one Bactec™ 12B bottle, and one Bactec™ 12B bottle with NAP. After reincubation, the inoculated growth bottle shows an increase in growth index, while the bottle containing NAP shows no increase. The organism cultured from the sputum is most likely Mycobacterium: a marinum b kansasii c tuberculosis d aviurmn-intracellulare

    c tuberculosis

  • 21

    on a culture suspected to be Mycobacterium tuberculosis, the most important test to perform is: a catalase production b tellurite reduction c Tween® 80 hydrolysis d_ niacin production

    d_ niacin production

  • 22

    A positive niacin test is most characteristic of Mycobacterium: chelonae marinum tuberculosis xenopt

    tuberculosis

  • 23

    Characteristics necessary for the definitive identification of Mycobacterium tuberculosis are: a buff color, slow growth at 37°C, niacin production-positive, nitrate reduction-negative b rough colony, slow growth at 37°C, nonpigmented c rough, nonpigmented colony, cording positive, niacin production-negative, catalase-negative at pit 7/68°C d_ rough, nonpigmented colony, slow growth at 37°C, niacin production-positive, nitrate reduction-positive

    d_ rough, nonpigmented colony, slow growth at 37°C, niacin production-positive, nitrate reduction-positive

  • 24

    A 2-week-old culture of a urine specimen produced a few colonies of acid-fast bacilli, which were rough and nonpigmented. The niacin test was weakly positive and the nitrate test was positive. Which of the following is the most appropriate action when a presumptive identification has been requested as soon as possible? a report the organism as presumptive Mycobacterium tuberculosis b_ wait a few days and repeat the niacin test; report presumptive Mycobacterium tuberculosis if the test is more strongly positive c subculture the organism and set up the routine battery of biochemicals; notify the physician that results will not be available for 3 weeks d set up a thiophene-2-carboxylic acid hydrazide (T*H); if the organism is sensitive, report Mycobacterium bovis

    b_ wait a few days and repeat the niacin test; report presumptive Mycobacterium tuberculosis if the test is more strongly positive

  • 25

    The disease-producing capacity of Mycobacterium tuberculosis depends primarily upon: a production of exotoxin b production of endotoxin ¢ capacity to withstand intracellular digestion by macrophages d lack of susceptibility to the myeloperoxidase system

    ¢ capacity to withstand intracellular digestion by macrophages

  • 26

    Which species of Mycobacterium includes a BCG strain used for vaccination against tuberculosis? a tuberculosis b bovis c kansasii d_ fortuitum/chelonae complex

    b bovis

  • 27

    AFB smear positive respiratory specimens may be reliably identified as Mycobacterium tuberculosis the same day the smear was read by: a cording seen on the AFB smear b nucleic acid amplification c QuantiFERON®-TB test [-7) DNA probes

    b nucleic acid amplification

  • 28

    A physician calls the laboratory to verify a result of Mycobacterium tuberculosis, stating that the clinical history of the patient is not compatible with tuberculosis. On review of the patient’s culture, the smear was negative and the culture became positive at 5 weeks in the broth culture only. Additionally, it was determined that another patient’s specimen that was processed the same day was 4+ AFB on smear and the culture was positive at 10 days for Mycobacterium tuberculosis. The lab should: a include a positive control when processing specimens b perform molecular fingerprinting on both isolates c repeat the nucleic acid probe d set up susceptibility tests

    b perform molecular fingerprinting on both isolates

  • 29

    Which of the following is considered a primary drug for the treatment of Mycobacterium tuberculosis? a rifampin b kanamycin c rifabutin d ethionamide

    a rifampin

  • 30

    An unusual number of Mycobacterium gordonae have been isolated. The most likely source is: an outbreak of infections due to Mycobacterium gordonae contamination by water organisms contarnination of commercial Lowenstein-Jensen tubes contamination of the specimen collection containers

    contamination by water organisms

  • 31

    When grown in the dark, yellow to orange pigmentation of the colonies is usually demonstrated by: a Mycobacterium tuberculosis b Mycobacterium kansasii c Mycobacterium fortuitum d Mycobacterium scrofulaceum

    d Mycobacterium scrofulaceum

  • 32

    the mycobacteria that produce a deep yellow or orange pigment both in the dark and light are: a photochromogens b scotochromogens ¢ nonchromogens d rapid growers

    b scotochromogens

  • 33

    Mycobacteria that produce pigment only after exposure to light are classified as: a photochromogens b scotochromogens c rapid growers d nonchromogens

    a photochromogens

  • 34

    Ina suspected case of Hansen disease (leprosy), a presumptive diagnosis is established by: a isolation of organisms on Lowenstein-Jensen medium b detection of weakly acid-fast bacilli in infected tissue c isolation of organisms in a cell culture d detection of niacin production by the isolated bacterium

    b detection of weakly acid-fast bacilli in infected tissue

  • 35

    The best medium for culture of Mycobacterium tuberculosis is: Bordet-Gengou agar Loeffler medium Lowenstein-Jensen medium cystine blood agar

    Lowenstein-Jensen medium

  • 36

    A 27-year-old scuba diver has an abrasion on his left thigh. A culture of this wound grew an acid-fast organism at 30°C. This isolate most likely is: a Mycobacterium chelonae b Mycobacterium marinum c Mycobacterium tuberculosis d Mycobacterium xenopi

    b Mycobacterium marinum

  • 37

    A nonchromogen that grows best at 42°C and is highly resistant to antibiotics is: a Mycobacterium chelonae b Mycobacterium marinum c Mycobacterium tuberculosis d Mycobacterium xenopi

    d Mycobacterium xenopi

  • 38

    Photochromogens produce pigment when: kept in the dark at 22°C exposed to light for 1 hour grown in the presence of CO2 incubated with x-ray film

    exposed to light for 1 hour

  • 39

    An acid-fast bacillus recovered from an induced sputum had the following characteristics: pigmentation: yellow in the dark, turning a deeper yellow-orange after 2 weeks of light exposure nitrate reduction: negative Tween® hydrolysis: positive at 5-10 days urease: negative Based on this information, the organism is most likely Mycobacterium: a_ scrofulaceum b gordonae c szulgai d flavescens

    b gordonae

  • 40

    Which of the following characteristics best distinguishes Mycobacterium scrofulaceum from Mycobacterium gordonae? a iron uptake b Tween® hydrolysis c good growth at 25°C d_ niacin production

    b Tween® hydrolysis

  • 41

    Differentiation of Mycobacterium avium from Mycobacterium intracellulare can be accomplished by: a nitrate reduction test b Tween® hydrolysis test ¢ resistance to 10 pg thiophene-2-carboxylic acid hydrazide (TCH) d DNA probe

    d DNA probe

  • 42

    Which one of the following species of Mycobacterium does not usually fluoresce on fluorochrome stain? a Mycobacterium fortuitum b Mycobacterium tuberculosis c Mycobacterium ulcerans d Mycobacterium bovis

    a Mycobacterium fortuitum

  • 43

    mycobacterial isolate that causes multiple skin nodules, grows at 30°C and requires hemin for growth is Mycobacterium: a marinum b genavense c haemophilum d xenopi

    c haemophilum

  • 44

    AFB smears of a lymph node biopsy from a child are positive. At the end of 8 weeks, the AFB cultures are no growth. To enhance the possibility of recovery of the causative organism, the technologist should: incubate the cultures an additional 4 weeks add mycobactin J to the media transfer the cultures to a 30°C incubator subculture the liquid culture to chocolate agar

    incubate the cultures an additional 4 weeks

  • 45

    The nitrate test for mycobacteria can be performed with a reagent impregnated paper strip or by the use of standard reagents. In order to quality control the test properly, which of the following should be used for a positive control? a Mycobacterium bovis b Mycobacterium gordonae ¢ Mycobacterium tuberculosis d Mycobacterium intracellulare

    c Mycobacterium tuberculosis

  • 46

    Virus transport medium containing penicillin, gentamicin and amphotericin is used to collect and transport specimens for virus culture because this medium: a_ enables rapid viral growth during the transport time b inhibits bacterial and fungal growth c destroys nonpathogenic viruses d inhibits complement-fixing antibodies

    b inhibits bacterial and fungal growth

  • 47

    Which of the following indicates the presence of a viral infection in tissue smears or biopsies? cytopathic effect intranuclear inclusions cell lysis mononuclear inflammatory cells

    intranuclear inclusions

  • 48

    Respiratory syncytial virus is best isolated using a(n): nasopharyngeal aspirate cough plate expectorated sputum throat swab

    nasopharyngeal aspirate

  • 49

    A urine specimen was submitted for isolation of cytomegalovirus (CMV). The urine was inoculated into human fibroblast tissue culture tubes. After 72 hours, no cytopathic effect was observed in the culture tubes. The mast appropriate course of action is to: incubate the culture tubes for 2-3 weeks longer request a fecal specimen as urine is inappropriate repeat the test using monkey kidney cell culture tubes request CMV serology as CMV cannot be isolated

    incubate the culture tubes for 2-3 weeks longer

  • 50

    The genus of virus associated with anogenital warts, cervical dysplasia and neoplasia is: herpes simplex virus papillomavirus cytomegalovirus coxsackievirus

    papillomavirus

  • 51

    Encephalitis is mest commonly associated with which of the following viruses? a Epstein-Barr b herpes simplex c coxsackie B d_ varicella zoster

    b herpes simplex

  • 52

    Colds and other acute respiratory diseases are most often associated with: Epstein-Barr virus adenovirus coxsackie B reovirus

    adenovirus

  • 53

    The Epstein-Barr virus is associated with which of the following? chickenpox Hodgkin lymphoma Burkitt lymphoma smallpox

    Burkitt lymphoma

  • 54

    Which organism fails to grow on artificial media or in cell cultures? a Chlamydia trachomatis b Neisseria gonorrhoeae c Treponema pallidum d_ herpes simplex virus

    c Treponema pallidum

  • 55

    Darkfield microscopy is can be used to visualize: a Pseudomonas aeruginosa b Streptococcus pneumoniae c« Treponema pallidum d_ Legioneila pneumophila

    c« Treponema pallidum

  • 56

    A Wright stain on a conjunctival smear from a neonate shows granular cytoplasmic perinuclear inclusions. This is mest indicative of: a Chlamydia trachomatis b herpes simplex virus c cytomegalovirus d_ varicella-zoster virus

    a Chlamydia trachomatis

  • 57

    lodine staining of a McCoy cell monolayer culture of a cervical swab reveals a large brown intracytoplasmic inclusion. What is the most likely infecting organism? a cytomegalovirus b Ehrlichia chaffeensis c« Chlamydia trachomatis d Rickettsia prowazekii

    c« Chlamydia trachomatis

  • 58

    Which compound, detected by Lugol iodine, is used in the nonimmunologic detection of Chlamydia trachomatis in cell culture? a DNA b RNA c glycogen d DNA polymerase

    c glycogen

  • 59

    Microorganisms resembling L-forms have been isolated from the blood of patients treated with antibiotics that: complex with flagellar protein interfere with cell membrane function inhibit protein synthesis interfere with cell wall synthesis

    interfere with cell wall synthesis

  • 60

    Relapsing fever in humans is caused by: a Borrelia recurrentis b Brucella abortus c Leptospira interrogans d Spirillum minus

    a Borrelia recurrentis

  • 61

    Psittacosis is transmissible to man via contact with: insects birds cattle dogs

    birds

  • 62

    Chlamydial infections have been implicated in: urethritis and conjunctivitis gastroenteritis and urethritis neonatal pneumonia and gastroenteritis neonatal meningitis and conjunctivitis

    urethritis and conjunctivitis

  • 63

    Mycoplasmas differ from bacteria in that they: a do not cause disease in humans b cannot grow in artificial inanimate media c lack cell walls d are not serologically antigenic

    c lack cell walls

  • 64

    jaundiced 7-year-old boy, with a history of playing in a pond in a rat-infested area, has a urine specimen submitted for a direct darkfield examination. Several spiral organisms are seen. Which of the following organisms would most likely be responsible for the patient’s condition? a Spirillum minus b Streptobacillus moniliformis c Listeria monocytogenes d_ Leptospira interrogans

    d Leptospira interrogans

  • 65

    A jaundiced 7-year-old boy, with a history of playing in a pond in a rat-infested area, has a urine specimen submitted for a direct dark-field examination. No organisms are seen in the specimen. Which medium should be inoculated in an attempt to isolate the suspected organism? a_blood cysteine dextrose b PPLO agar c Fletcher semisolid d chopped meat glucose

    c Fletcher semisolid

  • 66

    Which of the following is a growth requirement for the isolation of Leptospira? an atmosphere of 10% CO, an incubation temperature of 4°C 4-5 day incubation medium containing 10% serum plus fatty acids

    medium containing 10% serum plus fatty acids

  • 67

    Blood cultures from a case of suspected leptospiremia should be drawn: a_ between 10 pM and 2 AM b inthe first 7-10 days of infection c during febrile periods, late in the course of the disease d_ after the first 10 days of illness

    b inthe first 7-10 days of infection

  • 68

    What material should be used to prepare slides for direct smear examination for virus detection by special stains or FA technique? a_ vesicular fluid b leukocytes from the edge of the lesion c the top portion of the vesicle d_ epithelial cells from the base of the lesion

    d_ epithelial cells from the base of the lesion

  • 69

    A 29-year-old man is seen for recurrence of a purulent urethral discharge 10 days after the successful treatment of culture proven gonorrhea. The most likely etiology of his urethritis is: Mycoplasma hominis Chlamydia trachomatis Trichomonas vaginalis Neisseria gonorrhoeae

    Chlamydia trachomatis

  • 70

    Ureaplasma urealyticum are difficult to grow in the laboratory on routine media because of their requirement for: a_ sterols b horse blood ¢ ferric pyrophosphate d_ surfactant such as Tween® 80

    a_ sterols

  • 71

    A cell culture line used for the recovery of Chlamydia trachomatis from clinical specimens is: a Hela 229 b Hep-2 c BHK-21 d McCoy

    d McCoy

  • 72

    Rickettsiae infecting man multiply preferentially within which of the following cells? reticuloendothelial hepatic renal tubule endothelial

    endothelial

  • 73

    Artifacts found in a stool specimen that can be confused with ova or cysts are: partially digested meat fibers degenerated cells from the gastrointestinal mucosa dried chemical crystals pollen grains

    pollen grains

  • 74

    Polyvinyl alcohol used in the preparation of permanently stained smears of fecal material: concentrates eggs dissolves artifacts serves as an adhesive enhances stain penetration

    serves as an adhesive

  • 75

    The method of choice to detect Acanthamoeba sp from corneal ulcer scrapings is: a Novy, MacNeal and Nicolle (NNN) medium b culture on McCoy cells c direct exam d_ blood agar flooded with a 24-hour growth of E coli

    d_ blood agar flooded with a 24-hour growth of E coli

  • 76

    Primary amoebic encephalitis may be caused by: a Entamoeba coli b Dientamoeba fragilis c Endolimax nana d Naegleria fowleri

    d Naegleria fowleri

  • 77

    A formed stool is received in the laboratory at 3 AM for ova and parasite exam. The night shift technologist is certain that the workload will prevent examination of the specimen until 6 AM when the next shift arrives. The technologist should: a request that a new specimen be collected after 6 AM b perform a zinc sulfate floatation procedure for eggs and hold the remaining specimen at room temperature c examine a direct prep for trophozoites and freeze the remaining specimen d_ preserve the specimen in formalin until it can be examined

    d_ preserve the specimen in formalin until it can be examined

  • 78

    A batch of trichrome-stained slides for ova and parasite examination contains numerous minute crystals, which totally obscure the microscopic field. Which of the following measures is the most appropriate remedial action? change the Schaudinn fixative, remove coverslips and restain change the acid alcohol and restain remove coverslips and remount using fresh Permount™ or similar medium change the iodine alcohol solution to obtain a strong tea-colored solution, restain

    aa change the iodine alcohol solution to obtain a strong tea-colored solution, restain

  • 79

    The advantage of thick blood smears for malarial parasites is to: a improve staining of the organisms b improve detection of the organisms c remove RBC artifacts d remove platelets

    b improve detection of the organisms

  • 80

    Multifocal brain lesion in AIDS patients is commonly caused by: a Toxoplasma gondii b Pneumocystis jiroveci c Cryptosporidium parvum d Giardia lamblia

    a Toxoplasma gondii

  • 81

    A 44-year-old man was admitted to the hospital following a 2-week history of low-grade fever, malaise and anorexia. Examination of a Giemsa stain revealed many intraerythrocytic parasites. Further history revealed frequent camping trips near Martha’s Vineyard and Nantucket Island, but no travel outside the continental United States. This parasite could easily be confused with: a Trypanosoma cruzi b Trypanosoma rhodesiense/gambiense ¢ Plasmodium falciparum d Leishmania donovani

    ¢ Plasmodium falciparum

  • 82

    A patient is suspected of having amebic dysentery. Upon microscopic examination of a fresh fecal specimen for ova and parasites, the following data were obtained: a trophozoite of 25 ym progressive, unidirectional crawl evenly distributed peripheral chromatin finely granular cytoplasm This information probably indicates: a Entamoeba coli b Entamoeba histolytica c Endolimax nana da lodamoeba biitschlii

    b Entamoeba histolytica

  • 83

    Refer to the following image: Trophozoites of the cyst shown ahove are likely to: contain red blood cells have clear, pointed pseudoepodia contain few, if any, vacuoles have slow, undefined motility

    have slow, undefined motility

  • 84

    Refer to the following image: Upon finding the above in a fecal concentrate, the technologist should: a_ telephone the report of this pathogen to the physician immediately b review the fecal concentration carefully for the presence of other microorganisms that may be pathogenic c look for motile trophozoites d request a new specimen because of the presence of excessive pollen grains

    b review the fecal concentration carefully for the presence of other microorganisms that may be pathogenic

  • 85

    Refer to the following image: An inexperienced parasitology student may confuse the above organism with: a_ Entamoeba histolytica b Dientamoeba fragilis c Giardia lamblia d_ Trichomonas vaginalis

    a_ Entamoeba histolytica

  • 86

    Refer to the following image: This structure depicts a: cyst of a nonpathogenic amoeba trophozoite of a nonpathogenic amoeba cyst of a pathogenic amoeba trophozoite of a pathogenic amoeba

    cyst of a nonpathogenic amoeba

  • 87

    Refer to the following image: The organism depicted is a(n): amoeba flagellate filaria sporozoan

    flagellate

  • 88

    Refer to the following image: A 24-year-old woman, who just returned from vacationing in Russia, became ill with steatorrheal diarrhea. The above organism was found in her stool. The patient most likely is suffering from: a giardiasis b amebiasis € ascafiasis d balantidiasis

    a giardiasis

  • 89

    A liquid stool specimen is collected at 10:00 PM and brought to the laboratory for culture and ova and parasite examination. It is refrigerated until 10:10 AM the next day, when the physician requests that the technologist look for amoebic trophozoites. The best course of action would be to: a request a fresh specimen b perform a concentration on the original specimen c perform a trichrome stain on the original specimen d perform a saline wet mount on the original specimen

    a request a fresh specimen

  • 90

    Protozoan cysts are found in a wet mount of sediment from ethyl-acetate concentrated material. The cysts are without peripheral chromatin on the nuclear membrane. Each cyst has 4 nuclei, and each nucleus has a large karyosome, which appears as a refractive dot. These oval cysts are most likely: Endolimax nana Chilomastix mesnili Entamoeba histolytica Entamoeba hartmanni

    Endolimax nana

  • 91

    The term “internal autoinfection” is generally used in referring to infections with: a Ascaris lumbricoides b Necator americanus ¢ Trichuris trichiura d Strongyloides stercoralis

    d Strongyloides stercoralis

  • 92

    The best method to demonstrate the ova of Enterobius vermicularis is: a acid-ether concentration b cellophane tape preparation c formalin-ether concentration d zinc sulfate flotation

    b cellophane tape preparation

  • 93

    Proper collection of a sample for recovery of Enterobius vermicularis includes collecting: a 24-hour urine collection a first morning stool collection with proper preservative a scotch tape preparation from the perianal region peripheral blood from a finger

    a scotch tape preparation from the perianal region

  • 94

    fibrous skin nodule is removed from the back of a patient from Central America. A microfilaria seen upon microscopic exam of the nodule is: a Wuchereria bancrofti b Brugia malayi c Onchocerca volvulus d Loa loa

    c Onchocerca volvulus

  • 95

    Refer to the following image: The egg depicted above is most likely to be found in children suffering from: a diarrhea b constipation c perianal itching d stomach pain

    c perianal itching

  • 96

    Refer to the following illustration: The specimen of choice for finding the above parasite is: a stool b duodenal washing ¢ rectal swab d scotch tape preparation

    d scotch tape preparation

  • 97

    The examination of human feces is no help in the detection of: a Strongyloides stercoralis b Entamoeba histolytica ¢ Echinococcus granulosus d Ancylostoma duodenale

    ¢ Echinococcus granulosus

  • 98

    The causative agent of cysticercosis is: a Taenia solium b Taenia saginata ¢ Ascaris lumbricoides d Trichuris trichiura

    a Taenia solium

  • 99

    Organisms that can be easily identified to the species level from the ova in fecal specimens include: a Metagonimus yokogawat, Heterophyes heterophyes b Taenia solium, Taenia saginata c Necator americanus, Ancylostoma duodenale d Paragonimus westermani, Hymenolepis nana

    d Paragonimus westermani, Hymenolepis nana

  • 100

    The scolex of Taenia saginata has: a 4suckers b no suckers and 14 hooklets c 24 hooklets d 26-28 sucking discs

    a 4suckers

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    CC-3 ELECTROPHORESIS

    CC-3 ELECTROPHORESIS

    Yves Laure Pimentel · 41問 · 2年前

    CC-3 ELECTROPHORESIS

    CC-3 ELECTROPHORESIS

    41問 • 2年前
    Yves Laure Pimentel

    AUBF LAB SAFETY

    AUBF LAB SAFETY

    Yves Laure Pimentel · 87問 · 2年前

    AUBF LAB SAFETY

    AUBF LAB SAFETY

    87問 • 2年前
    Yves Laure Pimentel

    CSF 1

    CSF 1

    Yves Laure Pimentel · 100問 · 2年前

    CSF 1

    CSF 1

    100問 • 2年前
    Yves Laure Pimentel

    CSF 2

    CSF 2

    Yves Laure Pimentel · 82問 · 2年前

    CSF 2

    CSF 2

    82問 • 2年前
    Yves Laure Pimentel

    SEMEN 1

    SEMEN 1

    Yves Laure Pimentel · 100問 · 2年前

    SEMEN 1

    SEMEN 1

    100問 • 2年前
    Yves Laure Pimentel

    SEMEN 2

    SEMEN 2

    Yves Laure Pimentel · 7問 · 2年前

    SEMEN 2

    SEMEN 2

    7問 • 2年前
    Yves Laure Pimentel

    SYNOVIAL FLUID 1

    SYNOVIAL FLUID 1

    Yves Laure Pimentel · 100問 · 2年前

    SYNOVIAL FLUID 1

    SYNOVIAL FLUID 1

    100問 • 2年前
    Yves Laure Pimentel

    SYNOVIAL FLUID 2

    SYNOVIAL FLUID 2

    Yves Laure Pimentel · 6問 · 2年前

    SYNOVIAL FLUID 2

    SYNOVIAL FLUID 2

    6問 • 2年前
    Yves Laure Pimentel

    SEROUS FLUID

    SEROUS FLUID

    Yves Laure Pimentel · 25問 · 2年前

    SEROUS FLUID

    SEROUS FLUID

    25問 • 2年前
    Yves Laure Pimentel

    PLEURAL FLUID

    PLEURAL FLUID

    Yves Laure Pimentel · 44問 · 2年前

    PLEURAL FLUID

    PLEURAL FLUID

    44問 • 2年前
    Yves Laure Pimentel

    PERICARDIAL FLUID

    PERICARDIAL FLUID

    Yves Laure Pimentel · 18問 · 2年前

    PERICARDIAL FLUID

    PERICARDIAL FLUID

    18問 • 2年前
    Yves Laure Pimentel

    PERITONEAL FLUID/ ASCITIC FLUID

    PERITONEAL FLUID/ ASCITIC FLUID

    Yves Laure Pimentel · 30問 · 2年前

    PERITONEAL FLUID/ ASCITIC FLUID

    PERITONEAL FLUID/ ASCITIC FLUID

    30問 • 2年前
    Yves Laure Pimentel

    AMNIOTIC FLUID

    AMNIOTIC FLUID

    Yves Laure Pimentel · 92問 · 2年前

    AMNIOTIC FLUID

    AMNIOTIC FLUID

    92問 • 2年前
    Yves Laure Pimentel

    FECALYSIS

    FECALYSIS

    Yves Laure Pimentel · 80問 · 2年前

    FECALYSIS

    FECALYSIS

    80問 • 2年前
    Yves Laure Pimentel

    OTHER BODY FLUIDS

    OTHER BODY FLUIDS

    Yves Laure Pimentel · 89問 · 2年前

    OTHER BODY FLUIDS

    OTHER BODY FLUIDS

    89問 • 2年前
    Yves Laure Pimentel

    MTLBE- SUHO NOTES

    MTLBE- SUHO NOTES

    Yves Laure Pimentel · 86問 · 2年前

    MTLBE- SUHO NOTES

    MTLBE- SUHO NOTES

    86問 • 2年前
    Yves Laure Pimentel

    MTLBE..

    MTLBE..

    Yves Laure Pimentel · 35問 · 2年前

    MTLBE..

    MTLBE..

    35問 • 2年前
    Yves Laure Pimentel

    CC- CHROMATOGRAPHY

    CC- CHROMATOGRAPHY

    Yves Laure Pimentel · 19問 · 1年前

    CC- CHROMATOGRAPHY

    CC- CHROMATOGRAPHY

    19問 • 1年前
    Yves Laure Pimentel

    CC-OSMOMETRY

    CC-OSMOMETRY

    Yves Laure Pimentel · 14問 · 1年前

    CC-OSMOMETRY

    CC-OSMOMETRY

    14問 • 1年前
    Yves Laure Pimentel

    CC-ELECTROCHEMISTRY TECHNIQUES

    CC-ELECTROCHEMISTRY TECHNIQUES

    Yves Laure Pimentel · 43問 · 1年前

    CC-ELECTROCHEMISTRY TECHNIQUES

    CC-ELECTROCHEMISTRY TECHNIQUES

    43問 • 1年前
    Yves Laure Pimentel

    CC- CARBOHYDRATES

    CC- CARBOHYDRATES

    Yves Laure Pimentel · 49問 · 2年前

    CC- CARBOHYDRATES

    CC- CARBOHYDRATES

    49問 • 2年前
    Yves Laure Pimentel

    CC- DIABETES MELLITUS

    CC- DIABETES MELLITUS

    Yves Laure Pimentel · 97問 · 2年前

    CC- DIABETES MELLITUS

    CC- DIABETES MELLITUS

    97問 • 2年前
    Yves Laure Pimentel

    MAJOR LIPOPROTEINS

    MAJOR LIPOPROTEINS

    Yves Laure Pimentel · 57問 · 2年前

    MAJOR LIPOPROTEINS

    MAJOR LIPOPROTEINS

    57問 • 2年前
    Yves Laure Pimentel

    MINOR LIPOPROTEINS

    MINOR LIPOPROTEINS

    Yves Laure Pimentel · 63問 · 2年前

    MINOR LIPOPROTEINS

    MINOR LIPOPROTEINS

    63問 • 2年前
    Yves Laure Pimentel

    PROTEINS

    PROTEINS

    Yves Laure Pimentel · 37問 · 2年前

    PROTEINS

    PROTEINS

    37問 • 2年前
    Yves Laure Pimentel

    PLASMA PROTEINS 1

    PLASMA PROTEINS 1

    Yves Laure Pimentel · 91問 · 2年前

    PLASMA PROTEINS 1

    PLASMA PROTEINS 1

    91問 • 2年前
    Yves Laure Pimentel

    PLASMA PROTEINS 2 (ALPHA 2 REGION)

    PLASMA PROTEINS 2 (ALPHA 2 REGION)

    Yves Laure Pimentel · 98問 · 2年前

    PLASMA PROTEINS 2 (ALPHA 2 REGION)

    PLASMA PROTEINS 2 (ALPHA 2 REGION)

    98問 • 2年前
    Yves Laure Pimentel

    MISCELLANEOUS PROTEINS

    MISCELLANEOUS PROTEINS

    Yves Laure Pimentel · 61問 · 2年前

    MISCELLANEOUS PROTEINS

    MISCELLANEOUS PROTEINS

    61問 • 2年前
    Yves Laure Pimentel

    Liver Function Test 1

    Liver Function Test 1

    Yves Laure Pimentel · 100問 · 2年前

    Liver Function Test 1

    Liver Function Test 1

    100問 • 2年前
    Yves Laure Pimentel

    Liver Function Test 2

    Liver Function Test 2

    Yves Laure Pimentel · 96問 · 2年前

    Liver Function Test 2

    Liver Function Test 2

    96問 • 2年前
    Yves Laure Pimentel

    TUMOR MARKERS

    TUMOR MARKERS

    Yves Laure Pimentel · 33問 · 2年前

    TUMOR MARKERS

    TUMOR MARKERS

    33問 • 2年前
    Yves Laure Pimentel

    KIDNEY FUNCTION TESTS - NON PROTEIN NITROGEN & CREATININE

    KIDNEY FUNCTION TESTS - NON PROTEIN NITROGEN & CREATININE

    Yves Laure Pimentel · 41問 · 2年前

    KIDNEY FUNCTION TESTS - NON PROTEIN NITROGEN & CREATININE

    KIDNEY FUNCTION TESTS - NON PROTEIN NITROGEN & CREATININE

    41問 • 2年前
    Yves Laure Pimentel

    GLYCOGEN STORAGE DISEASES

    GLYCOGEN STORAGE DISEASES

    Yves Laure Pimentel · 17問 · 2年前

    GLYCOGEN STORAGE DISEASES

    GLYCOGEN STORAGE DISEASES

    17問 • 2年前
    Yves Laure Pimentel

    LIPID STORAGE DISEASES

    LIPID STORAGE DISEASES

    Yves Laure Pimentel · 14問 · 2年前

    LIPID STORAGE DISEASES

    LIPID STORAGE DISEASES

    14問 • 2年前
    Yves Laure Pimentel

    PROTEINS

    PROTEINS

    Yves Laure Pimentel · 71問 · 2年前

    PROTEINS

    PROTEINS

    71問 • 2年前
    Yves Laure Pimentel

    QUICK FIRE- ELECTRODES

    QUICK FIRE- ELECTRODES

    Yves Laure Pimentel · 7問 · 2年前

    QUICK FIRE- ELECTRODES

    QUICK FIRE- ELECTRODES

    7問 • 2年前
    Yves Laure Pimentel

    ELECTROLYTES

    ELECTROLYTES

    Yves Laure Pimentel · 10問 · 2年前

    ELECTROLYTES

    ELECTROLYTES

    10問 • 2年前
    Yves Laure Pimentel

    DRUGS

    DRUGS

    Yves Laure Pimentel · 27問 · 2年前

    DRUGS

    DRUGS

    27問 • 2年前
    Yves Laure Pimentel

    TOXIC AGENTS:

    TOXIC AGENTS:

    Yves Laure Pimentel · 12問 · 2年前

    TOXIC AGENTS:

    TOXIC AGENTS:

    12問 • 2年前
    Yves Laure Pimentel

    HEMATOLOGY 1 (HEMATOPOIESIS 1)

    HEMATOLOGY 1 (HEMATOPOIESIS 1)

    Yves Laure Pimentel · 100問 · 2年前

    HEMATOLOGY 1 (HEMATOPOIESIS 1)

    HEMATOLOGY 1 (HEMATOPOIESIS 1)

    100問 • 2年前
    Yves Laure Pimentel

    HEMATOLOGY (HEMATOPOIESIS 2)

    HEMATOLOGY (HEMATOPOIESIS 2)

    Yves Laure Pimentel · 29問 · 2年前

    HEMATOLOGY (HEMATOPOIESIS 2)

    HEMATOLOGY (HEMATOPOIESIS 2)

    29問 • 2年前
    Yves Laure Pimentel

    hema diseases

    hema diseases

    Yves Laure Pimentel · 25問 · 2年前

    hema diseases

    hema diseases

    25問 • 2年前
    Yves Laure Pimentel

    AML

    AML

    Yves Laure Pimentel · 43問 · 2年前

    AML

    AML

    43問 • 2年前
    Yves Laure Pimentel

    GLOBIN SYNTHESIS

    GLOBIN SYNTHESIS

    Yves Laure Pimentel · 71問 · 2年前

    GLOBIN SYNTHESIS

    GLOBIN SYNTHESIS

    71問 • 2年前
    Yves Laure Pimentel

    HEMATOLOGY REFERENCE RANGES

    HEMATOLOGY REFERENCE RANGES

    Yves Laure Pimentel · 31問 · 2年前

    HEMATOLOGY REFERENCE RANGES

    HEMATOLOGY REFERENCE RANGES

    31問 • 2年前
    Yves Laure Pimentel

    DISORDERS OF PRIMARY HEMOSTASIS

    DISORDERS OF PRIMARY HEMOSTASIS

    Yves Laure Pimentel · 34問 · 2年前

    DISORDERS OF PRIMARY HEMOSTASIS

    DISORDERS OF PRIMARY HEMOSTASIS

    34問 • 2年前
    Yves Laure Pimentel

    PLATELET DISORDERS

    PLATELET DISORDERS

    Yves Laure Pimentel · 37問 · 2年前

    PLATELET DISORDERS

    PLATELET DISORDERS

    37問 • 2年前
    Yves Laure Pimentel

    CHARACTERISTICS OF CLOTTING FACTORS

    CHARACTERISTICS OF CLOTTING FACTORS

    Yves Laure Pimentel · 100問 · 2年前

    CHARACTERISTICS OF CLOTTING FACTORS

    CHARACTERISTICS OF CLOTTING FACTORS

    100問 • 2年前
    Yves Laure Pimentel

    4. CLASSIFICATION OF MACROPHAGE

    4. CLASSIFICATION OF MACROPHAGE

    Yves Laure Pimentel · 12問 · 2年前

    4. CLASSIFICATION OF MACROPHAGE

    4. CLASSIFICATION OF MACROPHAGE

    12問 • 2年前
    Yves Laure Pimentel

    3. NATURAL OR INNATE IMMUNITY

    3. NATURAL OR INNATE IMMUNITY

    Yves Laure Pimentel · 39問 · 2年前

    3. NATURAL OR INNATE IMMUNITY

    3. NATURAL OR INNATE IMMUNITY

    39問 • 2年前
    Yves Laure Pimentel

    5. INTERNAL DEFENSE:SOLUBLE FACTORS (ACUTE PHASE REACTANTS)

    5. INTERNAL DEFENSE:SOLUBLE FACTORS (ACUTE PHASE REACTANTS)

    Yves Laure Pimentel · 55問 · 2年前

    5. INTERNAL DEFENSE:SOLUBLE FACTORS (ACUTE PHASE REACTANTS)

    5. INTERNAL DEFENSE:SOLUBLE FACTORS (ACUTE PHASE REACTANTS)

    55問 • 2年前
    Yves Laure Pimentel

    6. PHAGOCYTOSIS Chemotaxis

    6. PHAGOCYTOSIS Chemotaxis

    Yves Laure Pimentel · 37問 · 2年前

    6. PHAGOCYTOSIS Chemotaxis

    6. PHAGOCYTOSIS Chemotaxis

    37問 • 2年前
    Yves Laure Pimentel

    8. NATURE OF ANTIGEN

    8. NATURE OF ANTIGEN

    Yves Laure Pimentel · 44問 · 2年前

    8. NATURE OF ANTIGEN

    8. NATURE OF ANTIGEN

    44問 • 2年前
    Yves Laure Pimentel

    9. MAJOR HISTOCOMPATIBILITY COMPLEX/HUMAN LEUKOCYTE ANTIGEN

    9. MAJOR HISTOCOMPATIBILITY COMPLEX/HUMAN LEUKOCYTE ANTIGEN

    Yves Laure Pimentel · 50問 · 2年前

    9. MAJOR HISTOCOMPATIBILITY COMPLEX/HUMAN LEUKOCYTE ANTIGEN

    9. MAJOR HISTOCOMPATIBILITY COMPLEX/HUMAN LEUKOCYTE ANTIGEN

    50問 • 2年前
    Yves Laure Pimentel

    1. HISTORY

    1. HISTORY

    Yves Laure Pimentel · 69問 · 2年前

    1. HISTORY

    1. HISTORY

    69問 • 2年前
    Yves Laure Pimentel

    2. TYPES OF IMMUNITY

    2. TYPES OF IMMUNITY

    Yves Laure Pimentel · 38問 · 2年前

    2. TYPES OF IMMUNITY

    2. TYPES OF IMMUNITY

    38問 • 2年前
    Yves Laure Pimentel

    7. PHAGOCYTOSIS Engulfment and Digestion

    7. PHAGOCYTOSIS Engulfment and Digestion

    Yves Laure Pimentel · 21問 · 2年前

    7. PHAGOCYTOSIS Engulfment and Digestion

    7. PHAGOCYTOSIS Engulfment and Digestion

    21問 • 2年前
    Yves Laure Pimentel

    8. PHAGOCYTOSIS EXOCYTOSIS

    8. PHAGOCYTOSIS EXOCYTOSIS

    Yves Laure Pimentel · 15問 · 2年前

    8. PHAGOCYTOSIS EXOCYTOSIS

    8. PHAGOCYTOSIS EXOCYTOSIS

    15問 • 2年前
    Yves Laure Pimentel

    10. DISEASE ASSOCIATED TO HUMAN LEUKOCYTE ANTIGEN (henry)

    10. DISEASE ASSOCIATED TO HUMAN LEUKOCYTE ANTIGEN (henry)

    Yves Laure Pimentel · 32問 · 2年前

    10. DISEASE ASSOCIATED TO HUMAN LEUKOCYTE ANTIGEN (henry)

    10. DISEASE ASSOCIATED TO HUMAN LEUKOCYTE ANTIGEN (henry)

    32問 • 2年前
    Yves Laure Pimentel

    11. TRANSPLANTATION IMMUNOLOGY

    11. TRANSPLANTATION IMMUNOLOGY

    Yves Laure Pimentel · 30問 · 2年前

    11. TRANSPLANTATION IMMUNOLOGY

    11. TRANSPLANTATION IMMUNOLOGY

    30問 • 2年前
    Yves Laure Pimentel

    2. LYMPHOID ORGANS

    2. LYMPHOID ORGANS

    Yves Laure Pimentel · 38問 · 2年前

    2. LYMPHOID ORGANS

    2. LYMPHOID ORGANS

    38問 • 2年前
    Yves Laure Pimentel

    3. CLUSTER OF DIFFERENTIATION

    3. CLUSTER OF DIFFERENTIATION

    Yves Laure Pimentel · 48問 · 2年前

    3. CLUSTER OF DIFFERENTIATION

    3. CLUSTER OF DIFFERENTIATION

    48問 • 2年前
    Yves Laure Pimentel

    4. T-CELL DIFFERENTIATION

    4. T-CELL DIFFERENTIATION

    Yves Laure Pimentel · 35問 · 2年前

    4. T-CELL DIFFERENTIATION

    4. T-CELL DIFFERENTIATION

    35問 • 2年前
    Yves Laure Pimentel

    5. T CELL IMMUNODEFICIENCIES

    5. T CELL IMMUNODEFICIENCIES

    Yves Laure Pimentel · 18問 · 2年前

    5. T CELL IMMUNODEFICIENCIES

    5. T CELL IMMUNODEFICIENCIES

    18問 • 2年前
    Yves Laure Pimentel

    6. STAGES IN B-CELL DIFFERENTIATION  PROPIMAP

    6. STAGES IN B-CELL DIFFERENTIATION  PROPIMAP

    Yves Laure Pimentel · 44問 · 2年前

    6. STAGES IN B-CELL DIFFERENTIATION  PROPIMAP

    6. STAGES IN B-CELL DIFFERENTIATION  PROPIMAP

    44問 • 2年前
    Yves Laure Pimentel

    7. B CELL IMMUNODEFICIENCIES

    7. B CELL IMMUNODEFICIENCIES

    Yves Laure Pimentel · 23問 · 2年前

    7. B CELL IMMUNODEFICIENCIES

    7. B CELL IMMUNODEFICIENCIES

    23問 • 2年前
    Yves Laure Pimentel

    8. COMBINED T CELL AND B CELL DEFICIENCIES

    8. COMBINED T CELL AND B CELL DEFICIENCIES

    Yves Laure Pimentel · 34問 · 2年前

    8. COMBINED T CELL AND B CELL DEFICIENCIES

    8. COMBINED T CELL AND B CELL DEFICIENCIES

    34問 • 2年前
    Yves Laure Pimentel

    9. COMPARISON OF T AND B CELLS

    9. COMPARISON OF T AND B CELLS

    Yves Laure Pimentel · 12問 · 2年前

    9. COMPARISON OF T AND B CELLS

    9. COMPARISON OF T AND B CELLS

    12問 • 2年前
    Yves Laure Pimentel

    10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

    10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

    Yves Laure Pimentel · 16問 · 2年前

    10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

    10. LABORATORY IDENTIFICATION OF LYMPHOCYTES

    16問 • 2年前
    Yves Laure Pimentel

    11. NATURAL KILLER CELLS OR THIRD POPULATION CELL

    11. NATURAL KILLER CELLS OR THIRD POPULATION CELL

    Yves Laure Pimentel · 15問 · 2年前

    11. NATURAL KILLER CELLS OR THIRD POPULATION CELL

    11. NATURAL KILLER CELLS OR THIRD POPULATION CELL

    15問 • 2年前
    Yves Laure Pimentel

    12. ANTIBODY

    12. ANTIBODY

    Yves Laure Pimentel · 79問 · 2年前

    12. ANTIBODY

    12. ANTIBODY

    79問 • 2年前
    Yves Laure Pimentel

    13. TYPES OF ANTIBODIES

    13. TYPES OF ANTIBODIES

    Yves Laure Pimentel · 97問 · 2年前

    13. TYPES OF ANTIBODIES

    13. TYPES OF ANTIBODIES

    97問 • 2年前
    Yves Laure Pimentel

    14. MONOCLONAL ANTIBODIES

    14. MONOCLONAL ANTIBODIES

    Yves Laure Pimentel · 11問 · 2年前

    14. MONOCLONAL ANTIBODIES

    14. MONOCLONAL ANTIBODIES

    11問 • 2年前
    Yves Laure Pimentel

    1. INTERLEUKINS

    1. INTERLEUKINS

    Yves Laure Pimentel · 23問 · 2年前

    1. INTERLEUKINS

    1. INTERLEUKINS

    23問 • 2年前
    Yves Laure Pimentel

    2. INTERFERONS

    2. INTERFERONS

    Yves Laure Pimentel · 28問 · 2年前

    2. INTERFERONS

    2. INTERFERONS

    28問 • 2年前
    Yves Laure Pimentel

    3. CYTOKINES IN THE INNATE AND ADAPTIVE IMMUNITY

    3. CYTOKINES IN THE INNATE AND ADAPTIVE IMMUNITY

    Yves Laure Pimentel · 8問 · 2年前

    3. CYTOKINES IN THE INNATE AND ADAPTIVE IMMUNITY

    3. CYTOKINES IN THE INNATE AND ADAPTIVE IMMUNITY

    8問 • 2年前
    Yves Laure Pimentel

    4. COMPLEMENT SYSTEM

    4. COMPLEMENT SYSTEM

    Yves Laure Pimentel · 19問 · 2年前

    4. COMPLEMENT SYSTEM

    4. COMPLEMENT SYSTEM

    19問 • 2年前
    Yves Laure Pimentel

    15. COMPLEMENT SYSTEM

    15. COMPLEMENT SYSTEM

    Yves Laure Pimentel · 76問 · 2年前

    15. COMPLEMENT SYSTEM

    15. COMPLEMENT SYSTEM

    76問 • 2年前
    Yves Laure Pimentel

    16. SYSTEM CONTROLS/COMPLEMENT REGULATION

    16. SYSTEM CONTROLS/COMPLEMENT REGULATION

    Yves Laure Pimentel · 21問 · 2年前

    16. SYSTEM CONTROLS/COMPLEMENT REGULATION

    16. SYSTEM CONTROLS/COMPLEMENT REGULATION

    21問 • 2年前
    Yves Laure Pimentel

    17. COMPLEMENT AND DISEASE STATES

    17. COMPLEMENT AND DISEASE STATES

    Yves Laure Pimentel · 29問 · 2年前

    17. COMPLEMENT AND DISEASE STATES

    17. COMPLEMENT AND DISEASE STATES

    29問 • 2年前
    Yves Laure Pimentel

    18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

    18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

    Yves Laure Pimentel · 19問 · 2年前

    18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

    18. IMMUNOLOGIC ASSAYS OF INDIVIDUAL COMPONENTS (CLASSICAL)

    19問 • 2年前
    Yves Laure Pimentel

    19. CYTOKINES

    19. CYTOKINES

    Yves Laure Pimentel · 22問 · 2年前

    19. CYTOKINES

    19. CYTOKINES

    22問 • 2年前
    Yves Laure Pimentel

    20. INTERLEUKINS

    20. INTERLEUKINS

    Yves Laure Pimentel · 24問 · 2年前

    20. INTERLEUKINS

    20. INTERLEUKINS

    24問 • 2年前
    Yves Laure Pimentel

    21. INTERFERONS, TNF, TGF, CHEMOKINE

    21. INTERFERONS, TNF, TGF, CHEMOKINE

    Yves Laure Pimentel · 21問 · 2年前

    21. INTERFERONS, TNF, TGF, CHEMOKINE

    21. INTERFERONS, TNF, TGF, CHEMOKINE

    21問 • 2年前
    Yves Laure Pimentel

    1. SERO

    1. SERO

    Yves Laure Pimentel · 54問 · 2年前

    1. SERO

    1. SERO

    54問 • 2年前
    Yves Laure Pimentel

    2. PRECIPITATION

    2. PRECIPITATION

    Yves Laure Pimentel · 38問 · 2年前

    2. PRECIPITATION

    2. PRECIPITATION

    38問 • 2年前
    Yves Laure Pimentel

    3. PASSIVE IMMUNODIFFUSION

    3. PASSIVE IMMUNODIFFUSION

    Yves Laure Pimentel · 17問 · 2年前

    3. PASSIVE IMMUNODIFFUSION

    3. PASSIVE IMMUNODIFFUSION

    17問 • 2年前
    Yves Laure Pimentel

    4. OUCHTERLONY

    4. OUCHTERLONY

    Yves Laure Pimentel · 15問 · 2年前

    4. OUCHTERLONY

    4. OUCHTERLONY

    15問 • 2年前
    Yves Laure Pimentel

    5. ELECTROPHORETIC TECHNIQUE

    5. ELECTROPHORETIC TECHNIQUE

    Yves Laure Pimentel · 9問 · 2年前

    5. ELECTROPHORETIC TECHNIQUE

    5. ELECTROPHORETIC TECHNIQUE

    9問 • 2年前
    Yves Laure Pimentel

    6. IMMUNOELECTROPHORESIS (DOUBLE DIFFUSION)

    6. IMMUNOELECTROPHORESIS (DOUBLE DIFFUSION)

    Yves Laure Pimentel · 11問 · 2年前

    6. IMMUNOELECTROPHORESIS (DOUBLE DIFFUSION)

    6. IMMUNOELECTROPHORESIS (DOUBLE DIFFUSION)

    11問 • 2年前
    Yves Laure Pimentel

    7. IMMUNOFIXATION ELECTROPHORESIS

    7. IMMUNOFIXATION ELECTROPHORESIS

    Yves Laure Pimentel · 29問 · 2年前

    7. IMMUNOFIXATION ELECTROPHORESIS

    7. IMMUNOFIXATION ELECTROPHORESIS

    29問 • 2年前
    Yves Laure Pimentel

    問題一覧

  • 1

    Crust from a cauliflower-like lesion on the hand exhibited brown spherical bodies 6-12 um in diameter when examined microscopically. After 3 weeks of incubation at room temperature, a slow-growing black mold grew on Sabouraud dextrose agar. Microscopic examination revealed Cladosporium, Phialophora and Fonsecaea types of sporulation. The probable identification of this organism is: a Fonsecaea pedrosoi b Pseudallescheria boydii c Phialephora verrucosa d Cladosporium carrionii

    a Fonsecaea pedrosoi

  • 2

    Pus from a draining fistula on a foot was submitted for culture. Gross examination of the specimen revealed the presence of a small (0.8 mm in diameter), yellowish, oval granule. Direct microscopic examination of the crushed granule showed hyphae 3-4 um in diameter and the presence of chlamydospores at the periphery. After 2 days a cottony, white mold was seen that turned gray with a gray to black reverse after a few days. When viewed microscopically, moderately large hyaline septate hyphae with long or short conidiophores, each with a single pear-shaped conidium, 5-7 x 8-10 pm, were seen. The most likely identification is: a_ Exophiala jeanselmei b Fonsecaea pedrosoi c Pseudallescheria boydii d Cladosporium carrionii

    c Pseudallescheria boydii

  • 3

    In processing clinical specimens and fungal isolates, laboratory workers may contract systemic fungal infections through: a inhalation b ingestion c skin contact d insect vector

    a inhalation

  • 4

    A sputum specimen received at 8 AM for an AFB smear reveals acid- fast bacilli. An additional sputum is submitted that afternoon. This specimen was concentrated by the NALC-sodium hydroxide method and inoculated on 2 Lowenstein-Jensen slants and held for 8 weeks at 35°C in 5%-10% CO», No growth occurs. ‘Ihe best explanation is that: the hypochlorite technique was not used improper specimen was submitted for culture improper media was used for culture cultures were held for an insufficient period of time

    improper specimen was submitted for culture

  • 5

    ‘The preferred carbon source for mycobacteria is: a_ glycerol b glucose c fatty acids d casein hydrolysate

    a_ glycerol

  • 6

    first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Lowenstein-Jensen slants are incubated in the dark at 35°C with 5%-10% CO,. The smears reveal acid-fast bacilli, and after 7 days no growth appears on the slants. The best explanation is: a improper specimen submitted b incorrect concentration procedure c exposure to CO, prevents growth d cultures held for insufficient length of time

    d cultures held for insufficient length of time

  • 7

    A first morning sputum specimen is received for acid-fast culture. The specimen is centrifuged, and the sediment is inoculated on 2 Lowenstein-Jensen slants, which are incubated at 35°C in 5%-10% CO. After 1 week, the slants show abundant growth over the entire surface. Stains reveal gram-negative bacilli. To avoid this problem: a utilize a medium that inhibits bacterial growth b add sodium hypochlorite to the sediment before inoculation c incubate the tubes at room temperature to retard bacterial growth d decontaminate the specimen with sodium hydroxide

    d decontaminate the specimen with sodium hydroxide

  • 8

    A first morning sputum is received for culture of acid-fast bacilli. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Sabouraud dextrose slants are incubated in the dark at 35°C with 5%-10% COy. The smears reveal acid-fast bacilli, but the slants show no growth after 8 weeks. The explanation is: a improper media used b incorrect concentration procedure used c improper specimen submitted d exposure to CO, prevents growth

    a improper media used

  • 9

    In reviewing the number of Mycobacterium isolates for the current year, it was noted that there were 76% fewer isolates than the previous year (115 vs 28). The technologist in charge of the area has documented that the quality control of media, reagents and stains has been acceptable and there has been no gross contamination of the cultures noted. The most appropriate course of action to pursue would be: a stop use of commercial media and produce in-house b change to different formulations of egg and agar based media c change over to the Bactec™ system for isolation of Mycobacterium d review the digestion and decontamination procedure

    an review the digestion and decontamination procedure

  • 10

    Which of the following combinations of media provides an egg base, agar base, and a selective egg or agar base media? a Lowenstein-Jensen, American Thoracic Society (ATS), Middlebrook 7H11 b Lowenstein-Jensen, Middlebrook 7H11, Lowenstein-Jensen Gruft c Middlebrook 7H10, Petragnani, Lowenstein-Jensen [-7 Middlebrook 7H10, Middlebrook 7H11, Mitchison 7H11

    b Lowenstein-Jensen, Middlebrook 7H11, Lowenstein-Jensen Gruft

  • 11

    Which of the following reagents should be used as a mucolytic, alkaline reagent for digestion and decontamination of a sputum for mycobacterial culture? a N-acetyl-L-cystine and NaOH b NaOH alone c zephiran-trisodium phosphate d_ oxalic acid

    a N-acetyl-L-cystine and NaOH

  • 12

    The function of N-acetyl-L-cysteine in the reagent for acid-fast digestion-decontamination procedure is to: a inhibit growth of normal respiratory flora b inhibit growth of fungi c neutralize the sodium hydroxide d_ liquefy the mucus

    d liquefy the mucus

  • 13

    When staining acid-fast bacilli with Truant auramine-rhodamine stain, potassium permanganate is used as a: a decolorizing agent b quenching agent c mordant d dye

    b quenching agent

  • 14

    Middlebrook 7H10 and 7H11 media must be refrigerated in the dark, and incubated in the dark as well. If these conditions are not met, the media may prove toxic for mycobacteria because: a_ carbon dioxide will be released b growth factors will be broken down c light destroys the ammonium sulfate d formaldehyde may be produced

    d formaldehyde may be produced

  • 15

    The method used for processing specimens for mycobacterial culture contaminated with Pseudomonas is: a N-acetyl-L-cystine and NaOH b NaOH ¢ zephiran-trisodium phosphate d_ oxalic acid

    d_ oxalic acid

  • 16

    An AEB broth culture is positive for acid-fast bacilli at 1 week while the agar slant shows no growth. The most likely explanation for this is: a_ the organism is a contaminant b AFB grow more rapidly in liquid media ¢ PANTA was added to the broth d_ the agar slant was incubated in 5% CO,

    b AFB grow more rapidly in liquid media

  • 17

    A bronchial washing is processed for acid-fast bacilli. Which of the following precautions should be taken in order to prevent infection of laboratory personnel? add an equal amount of NALC to the specimen process all specimens under ultraviolet light centrifuge specimen only after the addition of preservative process all specimens in a biological safety hood

    process all specimens in a biological safety hood

  • 18

    Tubercle bacilli are specifically stained by: crystal violet 1% acid fuchsin methylene blue carbol fuchsin

    carbol fuchsin

  • 19

    ‘The species of mycobacteria that will give a positive niacin test is Mycobacterium: a leprae b kansasii c fortuitum d_ tuberculosis

    d_ tuberculosis

  • 20

    A digested and decontaminated sputum is inoculated into a Bactec™ 12B bottle and incubated in air at 37°C, On day 14, a positive growth index is obtained, and the auramine-rhodamine stain is positive. Broth from the initial bottle is inoculated into one Bactec™ 12B bottle, and one Bactec™ 12B bottle with NAP. After reincubation, the inoculated growth bottle shows an increase in growth index, while the bottle containing NAP shows no increase. The organism cultured from the sputum is most likely Mycobacterium: a marinum b kansasii c tuberculosis d aviurmn-intracellulare

    c tuberculosis

  • 21

    on a culture suspected to be Mycobacterium tuberculosis, the most important test to perform is: a catalase production b tellurite reduction c Tween® 80 hydrolysis d_ niacin production

    d_ niacin production

  • 22

    A positive niacin test is most characteristic of Mycobacterium: chelonae marinum tuberculosis xenopt

    tuberculosis

  • 23

    Characteristics necessary for the definitive identification of Mycobacterium tuberculosis are: a buff color, slow growth at 37°C, niacin production-positive, nitrate reduction-negative b rough colony, slow growth at 37°C, nonpigmented c rough, nonpigmented colony, cording positive, niacin production-negative, catalase-negative at pit 7/68°C d_ rough, nonpigmented colony, slow growth at 37°C, niacin production-positive, nitrate reduction-positive

    d_ rough, nonpigmented colony, slow growth at 37°C, niacin production-positive, nitrate reduction-positive

  • 24

    A 2-week-old culture of a urine specimen produced a few colonies of acid-fast bacilli, which were rough and nonpigmented. The niacin test was weakly positive and the nitrate test was positive. Which of the following is the most appropriate action when a presumptive identification has been requested as soon as possible? a report the organism as presumptive Mycobacterium tuberculosis b_ wait a few days and repeat the niacin test; report presumptive Mycobacterium tuberculosis if the test is more strongly positive c subculture the organism and set up the routine battery of biochemicals; notify the physician that results will not be available for 3 weeks d set up a thiophene-2-carboxylic acid hydrazide (T*H); if the organism is sensitive, report Mycobacterium bovis

    b_ wait a few days and repeat the niacin test; report presumptive Mycobacterium tuberculosis if the test is more strongly positive

  • 25

    The disease-producing capacity of Mycobacterium tuberculosis depends primarily upon: a production of exotoxin b production of endotoxin ¢ capacity to withstand intracellular digestion by macrophages d lack of susceptibility to the myeloperoxidase system

    ¢ capacity to withstand intracellular digestion by macrophages

  • 26

    Which species of Mycobacterium includes a BCG strain used for vaccination against tuberculosis? a tuberculosis b bovis c kansasii d_ fortuitum/chelonae complex

    b bovis

  • 27

    AFB smear positive respiratory specimens may be reliably identified as Mycobacterium tuberculosis the same day the smear was read by: a cording seen on the AFB smear b nucleic acid amplification c QuantiFERON®-TB test [-7) DNA probes

    b nucleic acid amplification

  • 28

    A physician calls the laboratory to verify a result of Mycobacterium tuberculosis, stating that the clinical history of the patient is not compatible with tuberculosis. On review of the patient’s culture, the smear was negative and the culture became positive at 5 weeks in the broth culture only. Additionally, it was determined that another patient’s specimen that was processed the same day was 4+ AFB on smear and the culture was positive at 10 days for Mycobacterium tuberculosis. The lab should: a include a positive control when processing specimens b perform molecular fingerprinting on both isolates c repeat the nucleic acid probe d set up susceptibility tests

    b perform molecular fingerprinting on both isolates

  • 29

    Which of the following is considered a primary drug for the treatment of Mycobacterium tuberculosis? a rifampin b kanamycin c rifabutin d ethionamide

    a rifampin

  • 30

    An unusual number of Mycobacterium gordonae have been isolated. The most likely source is: an outbreak of infections due to Mycobacterium gordonae contamination by water organisms contarnination of commercial Lowenstein-Jensen tubes contamination of the specimen collection containers

    contamination by water organisms

  • 31

    When grown in the dark, yellow to orange pigmentation of the colonies is usually demonstrated by: a Mycobacterium tuberculosis b Mycobacterium kansasii c Mycobacterium fortuitum d Mycobacterium scrofulaceum

    d Mycobacterium scrofulaceum

  • 32

    the mycobacteria that produce a deep yellow or orange pigment both in the dark and light are: a photochromogens b scotochromogens ¢ nonchromogens d rapid growers

    b scotochromogens

  • 33

    Mycobacteria that produce pigment only after exposure to light are classified as: a photochromogens b scotochromogens c rapid growers d nonchromogens

    a photochromogens

  • 34

    Ina suspected case of Hansen disease (leprosy), a presumptive diagnosis is established by: a isolation of organisms on Lowenstein-Jensen medium b detection of weakly acid-fast bacilli in infected tissue c isolation of organisms in a cell culture d detection of niacin production by the isolated bacterium

    b detection of weakly acid-fast bacilli in infected tissue

  • 35

    The best medium for culture of Mycobacterium tuberculosis is: Bordet-Gengou agar Loeffler medium Lowenstein-Jensen medium cystine blood agar

    Lowenstein-Jensen medium

  • 36

    A 27-year-old scuba diver has an abrasion on his left thigh. A culture of this wound grew an acid-fast organism at 30°C. This isolate most likely is: a Mycobacterium chelonae b Mycobacterium marinum c Mycobacterium tuberculosis d Mycobacterium xenopi

    b Mycobacterium marinum

  • 37

    A nonchromogen that grows best at 42°C and is highly resistant to antibiotics is: a Mycobacterium chelonae b Mycobacterium marinum c Mycobacterium tuberculosis d Mycobacterium xenopi

    d Mycobacterium xenopi

  • 38

    Photochromogens produce pigment when: kept in the dark at 22°C exposed to light for 1 hour grown in the presence of CO2 incubated with x-ray film

    exposed to light for 1 hour

  • 39

    An acid-fast bacillus recovered from an induced sputum had the following characteristics: pigmentation: yellow in the dark, turning a deeper yellow-orange after 2 weeks of light exposure nitrate reduction: negative Tween® hydrolysis: positive at 5-10 days urease: negative Based on this information, the organism is most likely Mycobacterium: a_ scrofulaceum b gordonae c szulgai d flavescens

    b gordonae

  • 40

    Which of the following characteristics best distinguishes Mycobacterium scrofulaceum from Mycobacterium gordonae? a iron uptake b Tween® hydrolysis c good growth at 25°C d_ niacin production

    b Tween® hydrolysis

  • 41

    Differentiation of Mycobacterium avium from Mycobacterium intracellulare can be accomplished by: a nitrate reduction test b Tween® hydrolysis test ¢ resistance to 10 pg thiophene-2-carboxylic acid hydrazide (TCH) d DNA probe

    d DNA probe

  • 42

    Which one of the following species of Mycobacterium does not usually fluoresce on fluorochrome stain? a Mycobacterium fortuitum b Mycobacterium tuberculosis c Mycobacterium ulcerans d Mycobacterium bovis

    a Mycobacterium fortuitum

  • 43

    mycobacterial isolate that causes multiple skin nodules, grows at 30°C and requires hemin for growth is Mycobacterium: a marinum b genavense c haemophilum d xenopi

    c haemophilum

  • 44

    AFB smears of a lymph node biopsy from a child are positive. At the end of 8 weeks, the AFB cultures are no growth. To enhance the possibility of recovery of the causative organism, the technologist should: incubate the cultures an additional 4 weeks add mycobactin J to the media transfer the cultures to a 30°C incubator subculture the liquid culture to chocolate agar

    incubate the cultures an additional 4 weeks

  • 45

    The nitrate test for mycobacteria can be performed with a reagent impregnated paper strip or by the use of standard reagents. In order to quality control the test properly, which of the following should be used for a positive control? a Mycobacterium bovis b Mycobacterium gordonae ¢ Mycobacterium tuberculosis d Mycobacterium intracellulare

    c Mycobacterium tuberculosis

  • 46

    Virus transport medium containing penicillin, gentamicin and amphotericin is used to collect and transport specimens for virus culture because this medium: a_ enables rapid viral growth during the transport time b inhibits bacterial and fungal growth c destroys nonpathogenic viruses d inhibits complement-fixing antibodies

    b inhibits bacterial and fungal growth

  • 47

    Which of the following indicates the presence of a viral infection in tissue smears or biopsies? cytopathic effect intranuclear inclusions cell lysis mononuclear inflammatory cells

    intranuclear inclusions

  • 48

    Respiratory syncytial virus is best isolated using a(n): nasopharyngeal aspirate cough plate expectorated sputum throat swab

    nasopharyngeal aspirate

  • 49

    A urine specimen was submitted for isolation of cytomegalovirus (CMV). The urine was inoculated into human fibroblast tissue culture tubes. After 72 hours, no cytopathic effect was observed in the culture tubes. The mast appropriate course of action is to: incubate the culture tubes for 2-3 weeks longer request a fecal specimen as urine is inappropriate repeat the test using monkey kidney cell culture tubes request CMV serology as CMV cannot be isolated

    incubate the culture tubes for 2-3 weeks longer

  • 50

    The genus of virus associated with anogenital warts, cervical dysplasia and neoplasia is: herpes simplex virus papillomavirus cytomegalovirus coxsackievirus

    papillomavirus

  • 51

    Encephalitis is mest commonly associated with which of the following viruses? a Epstein-Barr b herpes simplex c coxsackie B d_ varicella zoster

    b herpes simplex

  • 52

    Colds and other acute respiratory diseases are most often associated with: Epstein-Barr virus adenovirus coxsackie B reovirus

    adenovirus

  • 53

    The Epstein-Barr virus is associated with which of the following? chickenpox Hodgkin lymphoma Burkitt lymphoma smallpox

    Burkitt lymphoma

  • 54

    Which organism fails to grow on artificial media or in cell cultures? a Chlamydia trachomatis b Neisseria gonorrhoeae c Treponema pallidum d_ herpes simplex virus

    c Treponema pallidum

  • 55

    Darkfield microscopy is can be used to visualize: a Pseudomonas aeruginosa b Streptococcus pneumoniae c« Treponema pallidum d_ Legioneila pneumophila

    c« Treponema pallidum

  • 56

    A Wright stain on a conjunctival smear from a neonate shows granular cytoplasmic perinuclear inclusions. This is mest indicative of: a Chlamydia trachomatis b herpes simplex virus c cytomegalovirus d_ varicella-zoster virus

    a Chlamydia trachomatis

  • 57

    lodine staining of a McCoy cell monolayer culture of a cervical swab reveals a large brown intracytoplasmic inclusion. What is the most likely infecting organism? a cytomegalovirus b Ehrlichia chaffeensis c« Chlamydia trachomatis d Rickettsia prowazekii

    c« Chlamydia trachomatis

  • 58

    Which compound, detected by Lugol iodine, is used in the nonimmunologic detection of Chlamydia trachomatis in cell culture? a DNA b RNA c glycogen d DNA polymerase

    c glycogen

  • 59

    Microorganisms resembling L-forms have been isolated from the blood of patients treated with antibiotics that: complex with flagellar protein interfere with cell membrane function inhibit protein synthesis interfere with cell wall synthesis

    interfere with cell wall synthesis

  • 60

    Relapsing fever in humans is caused by: a Borrelia recurrentis b Brucella abortus c Leptospira interrogans d Spirillum minus

    a Borrelia recurrentis

  • 61

    Psittacosis is transmissible to man via contact with: insects birds cattle dogs

    birds

  • 62

    Chlamydial infections have been implicated in: urethritis and conjunctivitis gastroenteritis and urethritis neonatal pneumonia and gastroenteritis neonatal meningitis and conjunctivitis

    urethritis and conjunctivitis

  • 63

    Mycoplasmas differ from bacteria in that they: a do not cause disease in humans b cannot grow in artificial inanimate media c lack cell walls d are not serologically antigenic

    c lack cell walls

  • 64

    jaundiced 7-year-old boy, with a history of playing in a pond in a rat-infested area, has a urine specimen submitted for a direct darkfield examination. Several spiral organisms are seen. Which of the following organisms would most likely be responsible for the patient’s condition? a Spirillum minus b Streptobacillus moniliformis c Listeria monocytogenes d_ Leptospira interrogans

    d Leptospira interrogans

  • 65

    A jaundiced 7-year-old boy, with a history of playing in a pond in a rat-infested area, has a urine specimen submitted for a direct dark-field examination. No organisms are seen in the specimen. Which medium should be inoculated in an attempt to isolate the suspected organism? a_blood cysteine dextrose b PPLO agar c Fletcher semisolid d chopped meat glucose

    c Fletcher semisolid

  • 66

    Which of the following is a growth requirement for the isolation of Leptospira? an atmosphere of 10% CO, an incubation temperature of 4°C 4-5 day incubation medium containing 10% serum plus fatty acids

    medium containing 10% serum plus fatty acids

  • 67

    Blood cultures from a case of suspected leptospiremia should be drawn: a_ between 10 pM and 2 AM b inthe first 7-10 days of infection c during febrile periods, late in the course of the disease d_ after the first 10 days of illness

    b inthe first 7-10 days of infection

  • 68

    What material should be used to prepare slides for direct smear examination for virus detection by special stains or FA technique? a_ vesicular fluid b leukocytes from the edge of the lesion c the top portion of the vesicle d_ epithelial cells from the base of the lesion

    d_ epithelial cells from the base of the lesion

  • 69

    A 29-year-old man is seen for recurrence of a purulent urethral discharge 10 days after the successful treatment of culture proven gonorrhea. The most likely etiology of his urethritis is: Mycoplasma hominis Chlamydia trachomatis Trichomonas vaginalis Neisseria gonorrhoeae

    Chlamydia trachomatis

  • 70

    Ureaplasma urealyticum are difficult to grow in the laboratory on routine media because of their requirement for: a_ sterols b horse blood ¢ ferric pyrophosphate d_ surfactant such as Tween® 80

    a_ sterols

  • 71

    A cell culture line used for the recovery of Chlamydia trachomatis from clinical specimens is: a Hela 229 b Hep-2 c BHK-21 d McCoy

    d McCoy

  • 72

    Rickettsiae infecting man multiply preferentially within which of the following cells? reticuloendothelial hepatic renal tubule endothelial

    endothelial

  • 73

    Artifacts found in a stool specimen that can be confused with ova or cysts are: partially digested meat fibers degenerated cells from the gastrointestinal mucosa dried chemical crystals pollen grains

    pollen grains

  • 74

    Polyvinyl alcohol used in the preparation of permanently stained smears of fecal material: concentrates eggs dissolves artifacts serves as an adhesive enhances stain penetration

    serves as an adhesive

  • 75

    The method of choice to detect Acanthamoeba sp from corneal ulcer scrapings is: a Novy, MacNeal and Nicolle (NNN) medium b culture on McCoy cells c direct exam d_ blood agar flooded with a 24-hour growth of E coli

    d_ blood agar flooded with a 24-hour growth of E coli

  • 76

    Primary amoebic encephalitis may be caused by: a Entamoeba coli b Dientamoeba fragilis c Endolimax nana d Naegleria fowleri

    d Naegleria fowleri

  • 77

    A formed stool is received in the laboratory at 3 AM for ova and parasite exam. The night shift technologist is certain that the workload will prevent examination of the specimen until 6 AM when the next shift arrives. The technologist should: a request that a new specimen be collected after 6 AM b perform a zinc sulfate floatation procedure for eggs and hold the remaining specimen at room temperature c examine a direct prep for trophozoites and freeze the remaining specimen d_ preserve the specimen in formalin until it can be examined

    d_ preserve the specimen in formalin until it can be examined

  • 78

    A batch of trichrome-stained slides for ova and parasite examination contains numerous minute crystals, which totally obscure the microscopic field. Which of the following measures is the most appropriate remedial action? change the Schaudinn fixative, remove coverslips and restain change the acid alcohol and restain remove coverslips and remount using fresh Permount™ or similar medium change the iodine alcohol solution to obtain a strong tea-colored solution, restain

    aa change the iodine alcohol solution to obtain a strong tea-colored solution, restain

  • 79

    The advantage of thick blood smears for malarial parasites is to: a improve staining of the organisms b improve detection of the organisms c remove RBC artifacts d remove platelets

    b improve detection of the organisms

  • 80

    Multifocal brain lesion in AIDS patients is commonly caused by: a Toxoplasma gondii b Pneumocystis jiroveci c Cryptosporidium parvum d Giardia lamblia

    a Toxoplasma gondii

  • 81

    A 44-year-old man was admitted to the hospital following a 2-week history of low-grade fever, malaise and anorexia. Examination of a Giemsa stain revealed many intraerythrocytic parasites. Further history revealed frequent camping trips near Martha’s Vineyard and Nantucket Island, but no travel outside the continental United States. This parasite could easily be confused with: a Trypanosoma cruzi b Trypanosoma rhodesiense/gambiense ¢ Plasmodium falciparum d Leishmania donovani

    ¢ Plasmodium falciparum

  • 82

    A patient is suspected of having amebic dysentery. Upon microscopic examination of a fresh fecal specimen for ova and parasites, the following data were obtained: a trophozoite of 25 ym progressive, unidirectional crawl evenly distributed peripheral chromatin finely granular cytoplasm This information probably indicates: a Entamoeba coli b Entamoeba histolytica c Endolimax nana da lodamoeba biitschlii

    b Entamoeba histolytica

  • 83

    Refer to the following image: Trophozoites of the cyst shown ahove are likely to: contain red blood cells have clear, pointed pseudoepodia contain few, if any, vacuoles have slow, undefined motility

    have slow, undefined motility

  • 84

    Refer to the following image: Upon finding the above in a fecal concentrate, the technologist should: a_ telephone the report of this pathogen to the physician immediately b review the fecal concentration carefully for the presence of other microorganisms that may be pathogenic c look for motile trophozoites d request a new specimen because of the presence of excessive pollen grains

    b review the fecal concentration carefully for the presence of other microorganisms that may be pathogenic

  • 85

    Refer to the following image: An inexperienced parasitology student may confuse the above organism with: a_ Entamoeba histolytica b Dientamoeba fragilis c Giardia lamblia d_ Trichomonas vaginalis

    a_ Entamoeba histolytica

  • 86

    Refer to the following image: This structure depicts a: cyst of a nonpathogenic amoeba trophozoite of a nonpathogenic amoeba cyst of a pathogenic amoeba trophozoite of a pathogenic amoeba

    cyst of a nonpathogenic amoeba

  • 87

    Refer to the following image: The organism depicted is a(n): amoeba flagellate filaria sporozoan

    flagellate

  • 88

    Refer to the following image: A 24-year-old woman, who just returned from vacationing in Russia, became ill with steatorrheal diarrhea. The above organism was found in her stool. The patient most likely is suffering from: a giardiasis b amebiasis € ascafiasis d balantidiasis

    a giardiasis

  • 89

    A liquid stool specimen is collected at 10:00 PM and brought to the laboratory for culture and ova and parasite examination. It is refrigerated until 10:10 AM the next day, when the physician requests that the technologist look for amoebic trophozoites. The best course of action would be to: a request a fresh specimen b perform a concentration on the original specimen c perform a trichrome stain on the original specimen d perform a saline wet mount on the original specimen

    a request a fresh specimen

  • 90

    Protozoan cysts are found in a wet mount of sediment from ethyl-acetate concentrated material. The cysts are without peripheral chromatin on the nuclear membrane. Each cyst has 4 nuclei, and each nucleus has a large karyosome, which appears as a refractive dot. These oval cysts are most likely: Endolimax nana Chilomastix mesnili Entamoeba histolytica Entamoeba hartmanni

    Endolimax nana

  • 91

    The term “internal autoinfection” is generally used in referring to infections with: a Ascaris lumbricoides b Necator americanus ¢ Trichuris trichiura d Strongyloides stercoralis

    d Strongyloides stercoralis

  • 92

    The best method to demonstrate the ova of Enterobius vermicularis is: a acid-ether concentration b cellophane tape preparation c formalin-ether concentration d zinc sulfate flotation

    b cellophane tape preparation

  • 93

    Proper collection of a sample for recovery of Enterobius vermicularis includes collecting: a 24-hour urine collection a first morning stool collection with proper preservative a scotch tape preparation from the perianal region peripheral blood from a finger

    a scotch tape preparation from the perianal region

  • 94

    fibrous skin nodule is removed from the back of a patient from Central America. A microfilaria seen upon microscopic exam of the nodule is: a Wuchereria bancrofti b Brugia malayi c Onchocerca volvulus d Loa loa

    c Onchocerca volvulus

  • 95

    Refer to the following image: The egg depicted above is most likely to be found in children suffering from: a diarrhea b constipation c perianal itching d stomach pain

    c perianal itching

  • 96

    Refer to the following illustration: The specimen of choice for finding the above parasite is: a stool b duodenal washing ¢ rectal swab d scotch tape preparation

    d scotch tape preparation

  • 97

    The examination of human feces is no help in the detection of: a Strongyloides stercoralis b Entamoeba histolytica ¢ Echinococcus granulosus d Ancylostoma duodenale

    ¢ Echinococcus granulosus

  • 98

    The causative agent of cysticercosis is: a Taenia solium b Taenia saginata ¢ Ascaris lumbricoides d Trichuris trichiura

    a Taenia solium

  • 99

    Organisms that can be easily identified to the species level from the ova in fecal specimens include: a Metagonimus yokogawat, Heterophyes heterophyes b Taenia solium, Taenia saginata c Necator americanus, Ancylostoma duodenale d Paragonimus westermani, Hymenolepis nana

    d Paragonimus westermani, Hymenolepis nana

  • 100

    The scolex of Taenia saginata has: a 4suckers b no suckers and 14 hooklets c 24 hooklets d 26-28 sucking discs

    a 4suckers