MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

53問 • 5ヶ月前

問題一覧

This provides immediate ID but a negative finding does not rule out mycotic infection because wrong area of collection may have been sampled and only few condia may be present

MICROSCOPIC STUDY OF FUNGI

Wet preparations are simple cover slipped preparations examined under what objective

LOW POWER

C'mon specimen sources for wet preparations

SKIN, SCRAPING, NAIL, HAIR, BIOPSY

Rapid and inexpensive stain for direct examination that clears tissues debris by digesting keratin

POTASSIUM HYDROXIDE

This component of KOH prevents crystallization

GLYCEROL

Concentration of KOH

10-15%

This component of KOH may be added for contrast

METHYLENE BLUE

Ratio of methylene blue for KOH

2:1

How many minutes do KOH are allow to stand

10-15 MIN, HEAT GENTLY

This speed up digestion eliminating the need of heating and also added to enhance penetration

DIMETHYL SULFOXIDE

What specimen is not recommended to used Dimethyl sulfoxide

HAIR, THIN SKIN SCALE

Fluorescent stain that binds chitin and cellulose viewed under UV light

CALCOFLUOR WHITE

Calcofluor white may added with what component for rapid detection

POTASSIUM HYDROXIDE

Calcofluor white uses what background

DARK BACKGROUND

What colour do calcofluor white imparts

FLUORESCENT APPLE-GREEN, BLUE-WHITE

Used for encapsulated yeasts that appears as a refractile clear halo around yeast cells

INDIA INK, NIGROSIN, NEGATIVE STAIN

What fungi is specifically used India Ink

CRYPTOCOCCUS NEOFORMAN

India Ink may be diluted with

WATER

Used for detection of intracellular fungi

WRIGHTS, GIEMSA

Wrights and Giemsa are useful for what fungi

HISTOPLASMA CAPSULATUM

What colour of the fungal elements imparts in wrights, giemsa, and periodic acid schiff stain

RED/PURPLE

What colour of the background requires in Wrights and Giemsa stain

GREEN

Special stain for tissue section that is not often performed

HEMATOXYLIN AND EOSIN

What colour do fungi appear in hematoxylin and eosin

PINKISH-BLUE

Sensitive stain that uses green background that stains fungal cell wall great for localizing yeast cells hyphae

PERIODIC ACID SCHIFF

The gold standard in histopath for fungi staining

METHENAMINE SILVER, GOMORI

What colour do fungi appear in gomoris stain

BLACK

What colour of the background do gomoris stain uses

LAVENDER GRAY

Specific to capsules that highlights mucopolysaccharide and cryptococcus neoformans

MEYERS MUCICARMINE

What colour do fungi imparts in meyers mucicarmine

ROSE-RED

Highly specific stain sensitive detection but too expensive

FLUORESCENT ANTIBODY STAINING

Toxic but the standard/permanent w/ detailed slide mounting fluid in mycology

LACTOPHENOL COTTON BLUE

Component of LCPB that Acts as fixative

LACTIC ACID

Component of LCPB that acts as fungicidal agent

PHENOL

Component of LCPB that stains chitin in fungal cell wall

COTTON BLUE

An Alternative mounting fluid that preserves good structure reducing shrinking and distortion

POLYVINYL ALCOHOL

Rapid method by teasing fungal elements with needles or slides but mmay distort morphology

TEASE MOUNT

It is pressed onto fungal colony that placed on slide with LCPB that preserves colony morphology

CELLULOSE TAPE PREPARATION

What are the disadvantages of Cellulose tape preparation

NOT STERILE, NOT FOR LONG TERM PRESERVATION, AEROSOL SPREAD OF CONIDIA

Inoculated with fungi and covers lipped allowing observation of delicate linkages between conidia and conidiophores preserving natural arrangement of spores that is used for fungi with fragile structures

MICROSLIDE CULTURE, AGAR BLOCK METHOD

Are of the agar block in microslide method

1 CM

Rapid screening test for Candida albicans

GERM TUBE TEST

Initial filamentous outgrowth yeast cell longer than the parent cell about half as wide w/ parallel sides and asexual reproductive structure

GERM TUBE

Candida albicans is positive when germ tube forms for how many hours after incubation in serum

2-3 HOUR

This is to maintain viable cultures for research or teaching and achieved by slowing fungal metabolism

PRESERVATION OF FUNGAL CULTURE

Preservation of fungal cultures can be achieved by decreasing

OXYGEN, NUTRIENT AVAILABILITY

Method of fungi preservation which requires 10 drops of the reagent absorbed by cotton and placed in tube or jar with cultures

FORMALDEHYDE METHOD

Method of preservation which seals cultures tubes and plates reducing evaporation

PARAFILM SEALING

Storage of parafilm sealing

6-12 MOS/BOTH TEMP

Method of preservation that covers slant with sterile mineral oil limiting oxygen which is anerobic but messy to handle

MINERAL OIL OVERLAY

Method of preservation used in research studies that is viable for 1-2 years

FREEZING

Freezing method of preservation are stored in what temperature

-20C/-70C

Method of preservation which cultures is suspended in 2 ML sterile distilled water which is simple, inexpensive, but effective

STERILE WATER METHOD

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