The usual concentration of formaldehyde as a fixative

Tissue remains in one container but reagents get changed at schedule interval

Automated tissue transfer processor where the cassettes are in the processing chamber while the different reagent are pumped into the chamber

Fixation primary aim is to preserve cell

Fixation secondary aim is to make tissues

Formaldehyde fixes tissue by forming

Type of fixative which is taken in and become part of the tissue

Type of fixative which is not taken in and it facilitates the removal of water forcross-links to form

Practical consideration which the specimen should be placed in fixative as soon as it is removed from the body

Volume of fixative must be placed in a tissue that is not yet fixed

Formalin diffuses into the tissue approximately

Important in maintaining the microenvironment of cells as it ensures that cells are morphologically clear (especially under microscope) ensuring that cells are intact

The best general fixative

Microscopy use in slightly hypertonic (400-450 mOsm)

Microscopy use in more or less isotonic (340 mOsm)

Hasten the penetration of formalin into the tissue

Increases the penetration of formaldehyde

Type of fixative made up of only one component /substance

Type of fixative made of 2 or more fixatives

Type of fixative that permits the general microscopic study of tissue structures. It Focuses on the anatomy of cells

Type of fixative that preserve the specific parts and particular microscopic structure. It preserves Nuclear and Cytoplasmic fixatives

Type of fixative that preserve chemical contents (CHO, glycogen, mucopolysaccharide)

To improve the demonstration of particular substances. For special staining techniques possible (with secondary fixative acting as a mordant) to ensure complete hardening and preservation

For grams staining; mordant is

Improves the staining, optical differentiation of diff parts of tissue

A form of secondary fixation whereby a primarily fixed tissue is placed in aqueous solution of 2.5-3% potassium dichromate for 24hours to act as mordant. It is for better staining effects and to aid in cytologic preservation of tissues

Process of removing excess fixative from the tissue after fixation in order to improve staining and remove artifacts from the tissues

Removal of black granular deposit using alcoholic iodine