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Long discussion 1

  • Jef Marc Valencia

    • 問題数 70 • 9/4/2024

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  • 1

    AST Systematic name

    E.C. 2.6.1.1

  • 2

    AST other name

    SERUM GLUTAMIC OXALOACETIC TRANSAMINASE

  • 3

    Substrate of AST

    ASPARTATE+A KETOGLUTARATE

  • 4

    Product of AST

    OXALOACETATE+GLUTAMATE

  • 5

    Widely distributed in human tissue with the Highest concentration in cardiac tissue, liver, & skeletal muscle

    AST

  • 6

    Evaluation of hepatocellular disorders & skeletal muscle involvement

    AST

  • 7

    isoenzymes of AST in the

    MITOCHONDRIA, CYTOPLASM

  • 8

    Mild degree of liver tissue injury: cytoplasmic isoenzyme is

    PREDOMINANT

  • 9

    Severe tissue damage: release of

    MITOCHONDRIAL ISOENZYME

  • 10

    most important cause of elevated transaminase activity in serum

    LIVER DISEASE

  • 11

    AST Elevations

    PULMONARY EMBOLISM, VIRAL HEPATITIS: UP TO 100x ULN, CIRRHOSIS: ~4x ULN, SKELETAL MUSCLE DISORDER: ~4-8x ULN, AMI

  • 12

    in AMI, AST Rise: Peak: Return:

    6-8 HOUR, 24 HOUR, 5 DAY

  • 13

    ALT systematic name

    E.C 2.6.1.2

  • 14

    ALT other name

    SERUM GLUTAMIC PYRUVIC TRANSAMINASE

  • 15

    ALT Substrate

    ALANINE+A KETOGLUTARATE

  • 16

    ALT Product

    PYRUVATE+GLUTAMATE

  • 17

    Distributed in many tissues, High concentrations in the liver (more liver – specific enzyme among the transferases)

    ALT

  • 18

    ALT has an isoenzyme

    CYTOPLASMIC

  • 19

    Evaluation of hepatic disorders (hepatocellular).

    ALT

  • 20

    In cases of progressive inflammatory liver diseases _is higher than_

    ALT, AST

  • 21

    (AST:ALT ratio) is also known as

    DE RITIS RATIO

  • 22

    (AST:ALT ratio) normally and also in viral hepatitis

    <1.0

  • 23

    (AST:ALT ratio) associated with cirrhosis

    >1.0 but <2.0

  • 24

    (AST:ALT ratio)associated with alcoholic hepatitis or hepatocellular carcinoma.

    >2.0

  • 25

    Acute hepatocellular injury:

    AST>ALT

  • 26

    longer half – life, so after 24hrs or second testing during monitoring, it becomes higher

    AST

  • 27

    Method of measurment in Transaminase reactions coupled to specific DH (dehydrogenase reactions) reactions.

    CONTINOUS MONITORING

  • 28

    Assay for AST activity which Coupled enzymatic reaction w/ MDH (indicator reaction). Monitor the conversion of NADH to NAD, which is directly proportional to the amount of AST in the body

    KARMEN

  • 29

    Optimal pH for Karmen in AST

    7.3-7.8

  • 30

    In karment method, it is Monitored at spectrophotometrically at

    340 NM

  • 31

    Aspartate + a – ketoglutarate (AST) Oxaloacetate + Glutamate What reaction

    ASSAY

  • 32

    Oxaloacetate + NADH + H (MDH) Malate + NAD What reaction

    INDICATOR

  • 33

    Error in AST which Increase in serum AST – AST activity in RBC 15x higher in the serum.

    HEMOLYSIS

  • 34

    AST activity is stable in serum for

    3-4 DAY

  • 35

    Assay for ALT activity indicator

    LDH

  • 36

    RR for AST

    5-30 u/L

  • 37

    RR for ALT

    6-37 u/L

  • 38

    Method which Coupling w/ 2,4 – DNPH (Reitman – Frankel).• Still feasible.• Phenylhydrazones of oxaloacetate & pyruvate are more chromogenic.

    COLORIMETRIC

  • 39

    Simple; limited but acceptable accuracy. Mostly used in research laboratories

    COLORIMETRIC

  • 40

    Product formed in colorimetric method_is measured at_

    STRONG BLUE, 505 NM

  • 41

    Creatine Kinase systematic name

    E.C. 2.7.3.2

  • 42

    major phosphorylated compound in muscle

    PHOSPHOCREATINE

  • 43

    Forward reaction requires pH_in ck

    9

  • 44

    reverse reaction requires optimum pH_in ck

    6.7

  • 45

    Catalyzes reversible phosphorylation of creatine by ATP

    CREATINE KINASE

  • 46

    CK is mostly found in

    STRIATED MUSCLE, BRAIN TISSUE, HEART TISSUE

  • 47

    Each sub unit of Ck has a MW

    40000

  • 48

    (brain type) CK1 (fastest in electrophoresis)

    CK-BB

  • 49

    (hybrid type) CK2 (2nd fastest in electrophoresis)

    CK-MB

  • 50

    (muscle type) CK3 (3rd fastest in electrophoresis)

    CK-MM

  • 51

    All three isoenzymes of Ck are found in the

    CELL CYTOSOL

  • 52

    4th isoenzyme • Located between the inner and outer membranes of the mitochondria • Differs immunologically & in electrophoretic mobility

    CK-MT

  • 53

    the major serum in healthy individuals.

    CK3

  • 54

    contains almost mostly of CKMM

    SKELETAL MUSCLE

  • 55

    sensitive indicator of AMI

    CREATINE KINASE

  • 56

    Disease in skeleteal muscle in CK

    MYASTHENIA GRAVIS, MULTIPLE SCLEROSIS, POLIOMYELITIS, PARKISON'S DISEASE

  • 57

    isoenzyme will be elevated in conditions such as cerebral ischemia and cerebrovascular ischemia where blood flow to the brain is insufficient

    CK-BB

  • 58

    the liver and the brain is swollen as a side effect of viralinfections

    REYE'S SYNDROME

  • 59

    results in CK-MM elevations because of the involvement of muscle tissue (increased membrane permeability), the effect of thyroid hormone on enzyme activity, and, possibly, the slower clearance of CK as a result of slowermetabolism.

    HYPOTHYROIDISM

  • 60

    rarely seen in the serum because Of its molecular size

    CK-BB

  • 61

    associated also with patients with carcinoma of various organs such as adenocarcinoma, lung tumors, tumors of the prostate, kidney breasts and ovary the CKBB can be a useful tumor marker

    CK-BB

  • 62

    the reduction of NADH to NAD ismonitored spectrophotometrically. The change in absorbance is proportional to the activity of CK.

    FORWARD REACTION

  • 63

    preferred in the laboratory because it is about six times faster than the forward reaction.The rate of NADPH formation is a measure and directly proportional to the CK activity

    OLIVER ROSALKI, REVERSE REACTION

  • 64

    Sources of error in Ck dtermination

    ADENYLATE KINASE EFFECT, HEMOLYSIS, LIGHT

  • 65

    RR in male ck

    15-160 u/L

  • 66

    RR in female Ck

    15-130 u/L

  • 67

    reference and the most useful method in CK. Detection of atypical bands • Bands are visualized by incubating the support with a concentrated CK assay using the reverse reaction • in hemodialysis, albumin becomes endogenously fluorescent (stimulate the CK-1 band) • Allows visualization of AK

    ELECTROPHORESIS

  • 68

    Potential for being more sensitive & precise than electrophoresis

    ION EXCHANGE CHROMATOGRAPHY

  • 69

    Measure the concentration of enzyme proteins rather than the enzyme activity. • Detects enzymatically inactive CK – 2 • Infarction can be detected earlier than the other methods. • Example: emergency cases

    IMMUNOASSAY

  • 70

    Anti – M or Anti – B antibodies • CK activity is measured before and after inhibition. • Residual activity - Anti – B inhibit or stop the activity of B subunit of CK-B. • Multiply by 2 for the activity of CKMM

    IMMUNOINHIBITION

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    問題数 82025/05/06

    PLATYHELMINTHS

    問題数 132025/05/06

    MCQ-CSF2

    問題数 242025/05/03

    TREMATODES

    問題数 72025/05/06

    INTRODUCTION

    問題数 362025/06/10

    INTRODUCTION TABLE

    問題数 412025/06/10

    CLASSFICATION OF ENZYME

    問題数 472025/06/11

    ENZYME VARIANTS

    問題数 412025/06/11

    FACTORS AFFECTING ENZYME ACTIVITY

    問題数 902025/06/12

    INTRODUCTION TO ENZYMOLOGY-MCQ

    問題数 412025/06/26

    CLASS AND VARIANT OF ENZYME-MCQ

    問題数 772025/06/26

    PITUITARY GLAND PART 2

    問題数 52025/07/04

    THYROID GLAND

    問題数 712025/07/04

    PARATHYROID GLAND

    問題数 122025/07/04

    ADRENAL GLAND

    問題数 772025/07/04

    SEX GLANDS

    問題数 352025/07/04

    OTHER GLANDS

    問題数 82025/07/04

    ACID BASE BALANCE

    問題数 512025/07/10

    TRACE ELEMENT

    問題数 792025/07/10

    VITAMINS

    問題数 272025/07/10

    TUMOR MARKERS

    問題数 252025/07/10

    TOXICOLOGY

    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26