問題一覧
1
Decalcification is a process that can be putted between what process of routine histo
FIXATION, DEHYDRATION
2
A process in which calcium or lime salts are removed from tissues
DECALCIFICATION, DEMINERALIZATION
3
Tissues that are performed in decalcification
BONE, TEETH, TUBERCULOUS LUNG, ATHEROSCLEROTIC VESSEL
4
What if the Histotech did not decalcify the specimen
DAMAGE TO MICROTOME KNIFE, FORMATION OF BONE DUST
5
Consequences of decalcification
DISTORTION TO TISSUE, SECTION FLOAT OFF DURING STAINING
6
Why alcohol fixatives cant be used in decalcified speciemen?
PREVENT DECALCIFICATION
7
Affects of staining when overtreated with acid
EOSIN PRODUCE DEEP BRICK RED
8
Factors affecting decalcificatoon
VOLUME, CONCENTRATION, TIME, TEMPERATURE
9
Factor in which rationof fluid to tissue volume is 20 to 1
VOLUME
10
Ratio of fluid to tissue to volume
20:1
11
Routine decalfication time
24-48 HOUR
12
Dense bone decalcification duration
14 DAY
13
What temp impair nuclear staining of Van Gieson stain for collagen fibers
37
14
This temp will undergo complete digestion
55
15
Optimal temperature for decalcification
18-30
16
Position of the specimen when ddcalcifying
SUSPENDED
17
Reagents for decalcification
ACID, CHELATING, ION EXCHANGE RESIN, ELECTRICAL IONIZATION
18
Chelating means
TO ENVELOP
19
Most common, stable, available and cheap decalcifying reag
ACID
20
Acids used in decalcification
NITRIC ACID, FORMIC ACID, HYDROCHLORIC ACID, TCA, SULFUROUS, CHROMIC ACID, CITRIC ACID
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The most common and fastest nitric acid
5-10% NITRIC ACID
22
Rapid acting and good nuclear staining. Less tissue destruction but requires fume hood
FORMOL-NITRIC ACID
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In formol-nitric acid, it lessen yellow tissue discoloration by adding?
5% SODIUM SULFATE, 1% UREA
24
Decalcifying and tissue block softener
PERENYI'S
25
Nitric acid which rapid about 12-24 hrs. with minimal distortion
10% AQUEOUS NITRIC ACID
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Most rapid nitric acid but have poor nuclear staining and acid must be removed by 3 changes of 70-90% of alcohol
PHLOROGLUCIN-NITRIC ACID
27
Nitric Acid which have silver impregnation of nerve fiber
DE CASTRO'S FLUID
28
The best general decalcifying agent which good for small pieces for bones, and teeth. Excellent nuclear and cytoplasmic staining
5% FORMIC ACID
29
Slower and greater tissue distortion
HCL
30
Good cytologic staining under HCL
VON EBNER'S FLUID
31
Chelating agent
EDTA
32
EDTA is an excellent bone decalcifying agent for
IMMUNOHISTOCHEMISTRY , ENZYME STUDY
33
Act asbboth decalcifying agent and tissue softener which does not interfere stains and distort tissues
EDTA
34
Edta in small specimen duration
1-3 WEEK
35
Edta on dense cortical bone
6-8 WEEK
36
Well preserved cellular detail, economical and faster decalcification
ION EXCHANGE RESIN
37
Resins uses?
AMMONIUM-SULFONATE POLYSTYRENE, FORMIC ACID
38
Dissolution of calcium by an acid
ION EXCHANGE RESIN
39
In ion exhange resin, calcified specimen which are _mm thick could be decalcified within _hrs.
2-3, 2-3
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Calcium ions are attracted to negative electrode (cathode)
ELECTROPHORESIS
41
Eletrolyte soln for electrophoresis
8% HCL, 10% FORMIC ACID
42
For soaking the block face of trimmed paraffijn blocks
TISSUE BLOCK SOFTENER
43
Tissue block softerns agents
PERENYI'S FLUID, 4% AQUEOUS PHENOL, 1% HCL IN 70% ALCOHOL, 2% HCL
44
May cause swelling or make tissues soapy
MOLLIFLEX
45
Removal/neutralization of the reags from the tissues
POST DECALCIFICATION
46
Post decalcificatio processes
WASHING OUT, NEUTRALIZATION, EDTA
47
Test for complete decalcification
PHYSICAL, RADIOLOGIC, CHEMICAL
48
Complete decalcification which includes touching, bending, pricking, probing
PHYSICAL
49
Complete test which ideal and reliable yet expensive and not applicable on mercuric chloride fixed tissues
RADIOLOGIC
50
Complete decalcification which is Simple reliable and recommend for routine purposes
CHEMICAL
51
Complete test which requires unprecipiration as this would indicate incomplete decalcification
CALCIUM OXALATE TEST