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DECALCIFICATION

  • Jef Marc Valencia

    • 問題数 51 • 8/30/2024

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  • 1

    Decalcification is a process that can be putted between what process of routine histo

    FIXATION, DEHYDRATION

  • 2

    A process in which calcium or lime salts are removed from tissues

    DECALCIFICATION, DEMINERALIZATION

  • 3

    Tissues that are performed in decalcification

    BONE, TEETH, TUBERCULOUS LUNG, ATHEROSCLEROTIC VESSEL

  • 4

    What if the Histotech did not decalcify the specimen

    DAMAGE TO MICROTOME KNIFE, FORMATION OF BONE DUST

  • 5

    Consequences of decalcification

    DISTORTION TO TISSUE, SECTION FLOAT OFF DURING STAINING

  • 6

    Why alcohol fixatives cant be used in decalcified speciemen?

    PREVENT DECALCIFICATION

  • 7

    Affects of staining when overtreated with acid

    EOSIN PRODUCE DEEP BRICK RED

  • 8

    Factors affecting decalcificatoon

    VOLUME, CONCENTRATION, TIME, TEMPERATURE

  • 9

    Factor in which rationof fluid to tissue volume is 20 to 1

    VOLUME

  • 10

    Ratio of fluid to tissue to volume

    20:1

  • 11

    Routine decalfication time

    24-48 HOUR

  • 12

    Dense bone decalcification duration

    14 DAY

  • 13

    What temp impair nuclear staining of Van Gieson stain for collagen fibers

    37

  • 14

    This temp will undergo complete digestion

    55

  • 15

    Optimal temperature for decalcification

    18-30

  • 16

    Position of the specimen when ddcalcifying

    SUSPENDED

  • 17

    Reagents for decalcification

    ACID, CHELATING, ION EXCHANGE RESIN, ELECTRICAL IONIZATION

  • 18

    Chelating means

    TO ENVELOP

  • 19

    Most common, stable, available and cheap decalcifying reag

    ACID

  • 20

    Acids used in decalcification

    NITRIC ACID, FORMIC ACID, HYDROCHLORIC ACID, TCA, SULFUROUS, CHROMIC ACID, CITRIC ACID

  • 21

    The most common and fastest nitric acid

    5-10% NITRIC ACID

  • 22

    Rapid acting and good nuclear staining. Less tissue destruction but requires fume hood

    FORMOL-NITRIC ACID

  • 23

    In formol-nitric acid, it lessen yellow tissue discoloration by adding?

    5% SODIUM SULFATE, 1% UREA

  • 24

    Decalcifying and tissue block softener

    PERENYI'S

  • 25

    Nitric acid which rapid about 12-24 hrs. with minimal distortion

    10% AQUEOUS NITRIC ACID

  • 26

    Most rapid nitric acid but have poor nuclear staining and acid must be removed by 3 changes of 70-90% of alcohol

    PHLOROGLUCIN-NITRIC ACID

  • 27

    Nitric Acid which have silver impregnation of nerve fiber

    DE CASTRO'S FLUID

  • 28

    The best general decalcifying agent which good for small pieces for bones, and teeth. Excellent nuclear and cytoplasmic staining

    5% FORMIC ACID

  • 29

    Slower and greater tissue distortion

    HCL

  • 30

    Good cytologic staining under HCL

    VON EBNER'S FLUID

  • 31

    Chelating agent

    EDTA

  • 32

    EDTA is an excellent bone decalcifying agent for

    IMMUNOHISTOCHEMISTRY , ENZYME STUDY

  • 33

    Act asbboth decalcifying agent and tissue softener which does not interfere stains and distort tissues

    EDTA

  • 34

    Edta in small specimen duration

    1-3 WEEK

  • 35

    Edta on dense cortical bone

    6-8 WEEK

  • 36

    Well preserved cellular detail, economical and faster decalcification

    ION EXCHANGE RESIN

  • 37

    Resins uses?

    AMMONIUM-SULFONATE POLYSTYRENE, FORMIC ACID

  • 38

    Dissolution of calcium by an acid

    ION EXCHANGE RESIN

  • 39

    In ion exhange resin, calcified specimen which are _mm thick could be decalcified within _hrs.

    2-3, 2-3

  • 40

    Calcium ions are attracted to negative electrode (cathode)

    ELECTROPHORESIS

  • 41

    Eletrolyte soln for electrophoresis

    8% HCL, 10% FORMIC ACID

  • 42

    For soaking the block face of trimmed paraffijn blocks

    TISSUE BLOCK SOFTENER

  • 43

    Tissue block softerns agents

    PERENYI'S FLUID, 4% AQUEOUS PHENOL, 1% HCL IN 70% ALCOHOL, 2% HCL

  • 44

    May cause swelling or make tissues soapy

    MOLLIFLEX

  • 45

    Removal/neutralization of the reags from the tissues

    POST DECALCIFICATION

  • 46

    Post decalcificatio processes

    WASHING OUT, NEUTRALIZATION, EDTA

  • 47

    Test for complete decalcification

    PHYSICAL, RADIOLOGIC, CHEMICAL

  • 48

    Complete decalcification which includes touching, bending, pricking, probing

    PHYSICAL

  • 49

    Complete test which ideal and reliable yet expensive and not applicable on mercuric chloride fixed tissues

    RADIOLOGIC

  • 50

    Complete decalcification which is Simple reliable and recommend for routine purposes

    CHEMICAL

  • 51

    Complete test which requires unprecipiration as this would indicate incomplete decalcification

    CALCIUM OXALATE TEST

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    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26