DECALCIFICATION

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Decalcification is a process that can be putted between what process of routine histo

FIXATION, DEHYDRATION

A process in which calcium or lime salts are removed from tissues

DECALCIFICATION, DEMINERALIZATION

Tissues that are performed in decalcification

BONE, TEETH, TUBERCULOUS LUNG, ATHEROSCLEROTIC VESSEL

What if the Histotech did not decalcify the specimen

DAMAGE TO MICROTOME KNIFE, FORMATION OF BONE DUST

Consequences of decalcification

DISTORTION TO TISSUE, SECTION FLOAT OFF DURING STAINING

Why alcohol fixatives cant be used in decalcified speciemen?

PREVENT DECALCIFICATION

Affects of staining when overtreated with acid

EOSIN PRODUCE DEEP BRICK RED

Factors affecting decalcificatoon

VOLUME, CONCENTRATION, TIME, TEMPERATURE

Factor in which rationof fluid to tissue volume is 20 to 1

VOLUME

Ratio of fluid to tissue to volume

20:1

Routine decalfication time

24-48 HOUR

Dense bone decalcification duration

14 DAY

What temp impair nuclear staining of Van Gieson stain for collagen fibers

This temp will undergo complete digestion

Optimal temperature for decalcification

18-30

Position of the specimen when ddcalcifying

SUSPENDED

Reagents for decalcification

ACID, CHELATING, ION EXCHANGE RESIN, ELECTRICAL IONIZATION

Chelating means

TO ENVELOP

Most common, stable, available and cheap decalcifying reag

ACID

Acids used in decalcification

NITRIC ACID, FORMIC ACID, HYDROCHLORIC ACID, TCA, SULFUROUS, CHROMIC ACID, CITRIC ACID

The most common and fastest nitric acid

5-10% NITRIC ACID

Rapid acting and good nuclear staining. Less tissue destruction but requires fume hood

FORMOL-NITRIC ACID

In formol-nitric acid, it lessen yellow tissue discoloration by adding?

5% SODIUM SULFATE, 1% UREA

Decalcifying and tissue block softener

PERENYI'S

Nitric acid which rapid about 12-24 hrs. with minimal distortion

10% AQUEOUS NITRIC ACID

Most rapid nitric acid but have poor nuclear staining and acid must be removed by 3 changes of 70-90% of alcohol

PHLOROGLUCIN-NITRIC ACID

Nitric Acid which have silver impregnation of nerve fiber

DE CASTRO'S FLUID

The best general decalcifying agent which good for small pieces for bones, and teeth. Excellent nuclear and cytoplasmic staining

5% FORMIC ACID

Slower and greater tissue distortion

HCL

Good cytologic staining under HCL

VON EBNER'S FLUID

Chelating agent

EDTA

EDTA is an excellent bone decalcifying agent for

IMMUNOHISTOCHEMISTRY, ENZYME STUDY

Act asbboth decalcifying agent and tissue softener which does not interfere stains and distort tissues

EDTA

Edta in small specimen duration

1-3 WEEK

Edta on dense cortical bone

6-8 WEEK

Well preserved cellular detail, economical and faster decalcification

ION EXCHANGE RESIN

Resins uses?

AMMONIUM-SULFONATE POLYSTYRENE, FORMIC ACID

Dissolution of calcium by an acid

ION EXCHANGE RESIN

In ion exhange resin, calcified specimen which are _mm thick could be decalcified within _hrs.

2-3, 2-3

Calcium ions are attracted to negative electrode (cathode)

ELECTROPHORESIS

Eletrolyte soln for electrophoresis

8% HCL, 10% FORMIC ACID

For soaking the block face of trimmed paraffijn blocks

TISSUE BLOCK SOFTENER

Tissue block softerns agents

PERENYI'S FLUID, 4% AQUEOUS PHENOL, 1% HCL IN 70% ALCOHOL, 2% HCL

May cause swelling or make tissues soapy

MOLLIFLEX

Removal/neutralization of the reags from the tissues

POST DECALCIFICATION

Post decalcificatio processes

WASHING OUT, NEUTRALIZATION, EDTA

Test for complete decalcification

PHYSICAL, RADIOLOGIC, CHEMICAL

Complete decalcification which includes touching, bending, pricking, probing

PHYSICAL

Complete test which ideal and reliable yet expensive and not applicable on mercuric chloride fixed tissues

RADIOLOGIC

Complete decalcification which is Simple reliable and recommend for routine purposes

CHEMICAL

Complete test which requires unprecipiration as this would indicate incomplete decalcification

CALCIUM OXALATE TEST

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