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DEHYDRATION

  • Jef Marc Valencia

    • 問題数 64 • 9/3/2024

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  • 1

    Removal of fixative and intracellular and extracellular water fron the specimen

    DEHYDRATION

  • 2

    Filling cavities of the tissue with paraffin

    INFILTRATION

  • 3

    Replacement of fixative and water with dehydrating agent in preparation for

    IMPREGNATION

  • 4

    The most affected step

    IMPREGNATION

  • 5

    Dehydrating agent

    ALCOHOL IN INCREASING STRENGTH

  • 6

    Alcohol prevent

    VIOLENT OSMOTIC CHANGE

  • 7

    Accelerated increase in alcohol may result the tissue to

    HARD, BRITTLE, SHRUNKEN, DIFFICULT TO CUT

  • 8

    Dehydration involves slow substitution of the water in the tissue with an

    ORGANIC SOLVENT

  • 9

    Characteristics of an ideal dehydrating solution

    RAPID WITHOUT SHRINKAGE OR DISTORTION, DO NOT EVAPORATE QUICKLY, DEHYDRATE FAT, DOES NOT HARDEN TISSUE, DOES NOT REMOVE STAIN, NON TOXIC, NON FLAMMABLE

  • 10

    Minimum fixative volume

    20x THE VOLUME OF TISSUE

  • 11

    Acetone can be used in long time t/f

    F

  • 12

    Do not dry out the tissues t/f

    T

  • 13

    may be used as long as it does not damage the proteins and miscible,to the fluids to be used subsequently

    WATER MISCIBLE, ANHYDROUS FLUID

  • 14

    Signs of water content

    MACERATED TISSUE, MILKY XYLENE, ANHYDROUS COPPER SULFATE TEST

  • 15

    + in anhydrous copper sulfate

    BLUE

  • 16

    Temperature in dehydration

    37

  • 17

    More delicate tissue (embryo) begins at__ ethanol

    30%

  • 18

    Typical dehydration sequence (4mm) ethanol/min

    70%/15, 90%/15, 100%/15, 100%/15, 100%/30, 100%/45

  • 19

    Orientation of the tissue must be

    OUTSIDE

  • 20

    Embryo mold must not be in

    SIDEVIEW

  • 21

    Best dehydrating agent, Recommended for routine dehydration

    ETHANOL

  • 22

    Ethanol advs excwpt for routine dehydration

    PENETRATES EASILY, USED FOR EYES AND EMBRYO, NON TOXIC

  • 23

    Ethanol may react with

    UNREDUCED OSMIUM TETROXIDE

  • 24

    Long use of ethanol may cause

    SHRINKAGE, HARDEN

  • 25

    Ethanol extracts more lipids than

    ACETONE

  • 26

    Ethanol extractss _ and other_from section

    METHYLENE BLUE, THIAZINE DYE

  • 27

    Ethanol is only slightly miscible to

    WAX

  • 28

    Can be used in Blood, touch preparations and smear preparation but it is toxic

    METHANOL

  • 29

    Used in plant and animal micro-techniques, Less shrinkage and distortion than ethanol

    BUTANOL

  • 30

    Recommended for tissues which do not require rapid processing , Miscible with paraffin

    BUTANOL

  • 31

    Give 2 butanol cons

    ODOROUS, SLOW ACTING

  • 32

    Butanol temp

    117.7

  • 33

    Odorous reag you must use?

    FUME HOOD

  • 34

    Universal solvent-acts as dehydrating and clearing agent

    TERTIARY BUTANOL

  • 35

    Tertiary butanol mixes well with

    WATER, ETHANOL, XYLENE, PARAFFIN

  • 36

    Temp where tertiary butanol solidifys

    25

  • 37

    Temp of Tertiary butanol

    82.8

  • 38

    Excellent substitute for ethanol, Less shrinkage and hardening than ethanol. It is also sufficiently water-free to use in place of absolute ethanol and Less expensive. Used in Microwave processing

    ISOPROPANOL

  • 39

    Isopropanol Cannot be used in the celloidin technic since _ is insoluble in it

    NITROCELLULOSE

  • 40

    Miscible with 90% alcohol, toluene and xylene Dissolves paraffin wax

    PENTANOL, AMYL ALCOHOL

  • 41

    Cons of amyl alcohol

    TOXIC, CANNOT BE USED IN POORLY VENTILATED ROOM, NOT MISCIBLE WITH WATER

  • 42

    Cheap, rapid • Does not extract methylene blue and other dyes from stained sections . May cause less shrinkage of specimen than ethanol

    ACETONE

  • 43

    Not reactive with Os0, remaining in specimen. • Miscible with most embedding resins.

    ACETONE

  • 44

    Acetone 2 cons

    FLAMMABLE, VOLATILE

  • 45

    Acetone may induced_in prolonged immersion

    BRITTLENESS

  • 46

    Best processing requires a graded series of a mixture of _ and _ before one can go into paraffin

    ACETONE, XYLENE

  • 47

    Acetone is a

    FIXATIVE, DEHYDRATING

  • 48

    Universal solvent--it dehydrates and clears. Does not harm tissue over long time periods and Faster dehydrant than ethanol

    DIOXANE, DIETHYLENE DIOXIDE

  • 49

    2 cons of Dioxane

    HIGHLY TOXIC, RIBBONS POORLY

  • 50

    Explosive products in dioxane wgen reused

    PEROXIDE

  • 51

    This where the tisue rriboon palced to fix its fold

    FLOATATION BATH

  • 52

    2 methods in dioxane

    GRAUPNER'S, WEISEBERGER'S

  • 53

    tissue is wrapped in a gauze bag and suspended in a bottle containing dioxane and a little anhydrous calcium oxide

    WEISEBERGER'S

  • 54

    Rapid dehydrating agent • Tissue may remain in it for months without injury

    CELLOSOLVE, ETHYLENE GLYCOL MONOETHYL ETHER

  • 55

    Tissues may be transferred from water or normal saline and stored for months without hardening or distortion • Avoids distortion and does not require graded dilutions

    CELLOSOLVE, ETHYLENE GLYCOL MONOETHYL ETHER

  • 56

    Cellusolve combust at

    43-48

  • 57

    Universal solvent-it dehydrates and clears • Miscible in all proportions with water, ether chloroform, acetone, and the hydrocarbons xylene, toluene, and benzene

    TETRAHYDROFURAN

  • 58

    Rapid without excessive shrinkage and hardening; easier cutting of sections • Low toxicity; low fire and explosion

    TETRAHYDROFURAN

  • 59

    6months exposure to tetrahydrofuran produces

    CONJUNCTIVAL IRRITATION

  • 60

    For electron microscopy Dehydrating agent: Transition fluid: Substitute:

    ETHANOL, PROPYLENE OXIDE, ACETONITRILE

  • 61

    softening agent for hard tissues

    GLYCEROL

  • 62

    acts as a softening agent for hard tissues like tendons, nail, dense connective tissues, and keratin masses

    PHENOL

  • 63

    _phenol is added to each 95% ethanol stations

    4%

  • 64

    This will impair subsequent step

    WATER

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    PITUITARY GLAND PART 2

    問題数 52025/07/04

    THYROID GLAND

    問題数 712025/07/04

    PARATHYROID GLAND

    問題数 122025/07/04

    ADRENAL GLAND

    問題数 772025/07/04

    SEX GLANDS

    問題数 352025/07/04

    OTHER GLANDS

    問題数 82025/07/04

    ACID BASE BALANCE

    問題数 512025/07/10

    TRACE ELEMENT

    問題数 792025/07/10

    VITAMINS

    問題数 272025/07/10

    TUMOR MARKERS

    問題数 252025/07/10

    TOXICOLOGY

    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26