Quiz Announcement ANALYTICAL METHODS &amp; INSTRUMENTATION

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Travels as electromagnetic wave

RADIANT ENERGY, ELECTROMAGNETIC ENERGY

the proportion of wavelength and electromagnetic energy is _

INVERSELY PROPORTIONAL

LAW OF COLORIMETRY the concentration of a solution for the known path length is directly proportional to its absorption of light.

BEER LAMBERT LAW

TYPES OF ELECTROMAGNETIC ENERGIES

COSMIC RAY, GAMMA RAY, X-RAY, VISIBLE, ULTRA VIOLET, INFRARED, MICROWAVE

Types of Cuvette for solutions that do not etch glass

BOROSILICATE GLASS

distance between two peaks as the light travels in a wavelike manne

WAVELENGTH

Types of Cuvette does not absorb UV radiation at wavelength below 320 nm

QUARTZ, PLASTIC

Types of Cuvette good for 340 nm and above (visible region)

ALUMINA SILICA GLASS

Visible-spectra Wavelength

340-700nm

An Internal Component of Spectro which measure light intensity by converting light signal into electrical signal

DETECTOR

Non-visible spectra below 340nm

ULTRAVIOLET REGION

Non visible spectra that above 700nm

INFRARED REGION

Type of detector composed of a film of light sensitive material (e.g. Selenium) on an iron plate with a transparent layer of silver

PHOTOCELL

Type of detector that when light passing through the semi-conductive metal layer falls upon the Selenium surface, electrons are released in proportion to the intensity of light and are collected to the silver layer to produce a negative charge

PHOTOCELL

Wavelength is expressed in

NANOMETER, ANGSTROM, MILLIMICRON

Type of detector that has as photosensitive material that gives off electrons when light energy strikes it

PHOTOTUBE, PHOTOEMISSIVE TUBE

Measurement of the wavelength and the intensity of electromagnetic radiation in the visible region of the spectrum.

COLORIMETRY

Used for identification and determination of concentrations of substances that absorb light

COLORIMETRY

Kinds of Colorimetry

VISUAL, PHOTOELECTRIC

Kind of Colorimetry Relies on visual acuity to determine end-point

VISUAL

Kind of Colorimetry primary analytical utility of spectrophotometry or filter photometry is the isolation of discreet portion of spectrum for the purposes of measurement

PHOTOELECTRIC

Kinds of Photoelectric Colorimetry

SPECTROPHOTOMETRY, FILTER PHOTOMETRY

Photoelectric Colorimetry where it make use of prisms and/or diffraction gratings as monochromator to disperse the radiant energy into a continuous spectrum

SPECTROPHOTOMETRY

Photoelectric Colorimetry where it make useof filters (interference or transmission) to isolate part of the spectrum

FILTER PHOTOMETRY

LAW OF COLORIMETRY The concentration of the solution is directly proportional to the amount of light absorbed and inversely proportional to the logarithm of transmitted light

BEER'S LAW

LAW OF COLORIMETRY Absorbance is directly proportional to the length of light path

BOUGUER'S LAW, LAMBERT'S LAW

An Internal Component of Spectro that provides a continuous spectrum of white light which can be separated at different wavelengths

LIGHT SOURCE

Most commonly used light source produces energy wavelength from 340 – 700 nm (visible region); used for moderately diluted solution

TUNGSTEN IODIDE LAMP

A light Source which Iodine used to prevent the decomposition on the vaporized tungsten from the hot filament of light

QUARTZ HALIDE LAMP

A light Source which provides energy source with high output in the UV range (down to 165 nm)

DEUTERIUM DISCHARGE LAMP

A light Source which used above 800 nm

INFRARED ENERGY SOURCE

Beer's Law formula Where A= a= b= c=

ABSORBANCE, MOLAR ABSORPTIVITY, LIGHT PATH OF THE SOLUTION, CONC OF SUBSTANCE

A light Source which emits narrow bands of energy at well-defined places in the spectrum (UV and visible)

MERCURY VAPOR LAMP

Glass prisms are for

VISIBLE REGION

are for the UV region in prism

QUARTZ PRISM

A type of monochromator that Rays of radiant energy bend (refract) around the sharp edges of the grooves and Extent of refraction varies with the wavelength

DIFFRACTION GRATING

A type of monochromator that the thickness of the layer determines the wavelength of energy transmitted and with Band pass of 10 – 20 nm

INTERFERENCE FILTER

2 Types of Double Beam Spectrophotometer

DOUBLE BEAM IN SPACE, DOUBLE BEAM IN TIME

w/ 2 photodetectors and all components are duplicated except the light source the beams of light pass-through different components but at the same time

DOUBLE BEAM IN SPACE

Light Source consists of a gas tight chamber Containing anode, a cylindrical Cathode and inert gas such as helium or argon

HOLLOW CATHODE

Light Source produces a continuous source of radiation which covers both the UV and visible range. This is not suited for routine application as problems result from stray light

XENON

An Internal Component of Spectro – isolates a narrow beam of radiant energy; prevents stray light from entering the monochromator

ENTRANCE SLIT

Defines the range of wavelengths transmitted and is calculated as width at more than half the maximum transmittance

MONOCHROMATOR BANDPASS

A type of monochromator that is Wedge-shaped pieces of glass, quartz, NaCl, that can be rotated

PRISM

A type of monochromator that disperses white light into a continuous spectrum of colors by refraction

PRISM

A type of monochromator most commonly used monochromator because it has better resolution.

DIFFRACTION GRATING

A type of monochromator device that has small parallel grooves or slits and acts as prism (refract white light) and has slit (diffract it into several spectra)

DIFFRACTION GRATING

Prism monochromator Principle

NON-LINEAR

Diffraction Grating Monochromator Principle

LINEAR

A type of monochromator that has Colored glass or colored gelatin sandwiched between two glass plates

TRANSMISSION FILTER

In TRANSMISSION FILTERS Light outside the transmission band is absorbed by the

COLORED MATERIAL

The Band Pass of TRANSMISSION FILTERS

35-50nm

A type of monochromator that is Semi-transparent silver films on both sides of dielectric such as MgF. It is not expensive but precise

INTERFERENCE FILTER

A type of monochromator that produces monochromatic light based on the principle of constructive interference of waves

INTERFERENCE FILTER

An Internal Component of Spectro with an Internal Dimension of 1 Cm that holds the solution in which the absorption is to be measured

ANALYTICAL CELL, CUVETTE, ABSORPTION CELL

Other name for Photocell

PHOTOVOLTAIC CELL, BARRIER LAYER CELL

Type of detector where it is a device whose electrical resistance decreases as the level of incident light is raised and do not require an external power source

PHOTOCONDUCTIVE TUBE, PHOTORESISTIVE TUBE

Type of detector that has Cadmium sulfide or cadmium selenide are the light-sensitive materials typically used for the visible region

PHOTOCONDUCTIVE TUBE, PHOTORESISTIVE TUBE

Type of detector that is capable of significantly amplifying a current and the cathode is a negative light-sensitive metal that absorbs light and emits electrons in proportion to the radiant energy that strikes the surface

PHOTOMULTIPLIER TUBE

Type of detector where electrons go to the dynodes, where electrons produce 4 – 6 additional electrons. The electrons are collected at a final electrode, the positive anode

PHOTOMULTIPLIER TUBE

Advantages of Photomultiplier tube

RAPID RESPONSE TIME, VERY SENSITIVE, LOW FATIGUE

An Internal Component of Spectro Where electrical energy from a detector is displayed

READ OUT DEVICES

A read out device where the output of the detector is used to drive a sensitive meter directly without further amplification

DIRECT READING SYSTEM

A read out device where the output of the detector is balanced against the output of a reference circuit

NULL POINT SYSTEM

A read out device where numerical display of absorbance or converted values of concentrations

DIGITAL READ OUT

A read out device where

MICROPROCESSOR

Type of Double Beam Spectrophotometer where All components are duplicated except the light source

DOUBLE BEAM IN SPACE

Type of Double Beam Spectrophotometer where beams of light pass through different components but at the same time

DOUBLE BEAM IN SPACE

Type of Double Beam Spectrophotometer that Uses a light beam chopper (a rotating wheel) – with alternate silvered sections and cut out sections, inserted after the exit slit.

DOUBLE BEAM IN TIME

It involves the measurement of emitted light when electrons in an atom become excited by heat energy produced by the flame. When these electrons return to their ground state, they emit light characteristic of the ions present.

FLAME PHOTOMETRY

Other name for flame photometry

FLAME EMISSION SPECTROPHOTOMETRY, FILTER PHOTOMETRY

It is used primarily to determine concentration of sodium, potassium or lithium since these alkali metals are easy to excite.

FLAME PHOTOMETRY

Color of alkali metals when excite letters only Sodium = Potassium = Lithium = Rubidium = Magnesium =

Y, V, R, R, B

A component of Flame Photometer mixture of hydrogen and oxygen gas

GAS

Gases in Flame Photometry

ACETYLENE, PROPANE, NATURAL GAS

Part of the Burner assembly where it draw sample into the flame

ASPIRATOR

Part of the Burner assembly that creates a fine spray of sample solution to be fed into the flame of the burner

ATOMIZER, NEBULIZER

Part of the Burner assembly that provides heat energy for excitation

FLAME

A type of Burner in Flame Photometry where aspirate sample directly into the flame, the gases are passed at high velocity over the end of the capillary suspended in the solution

TOTAL CONSUMPTION BURNER

A type of Burner in Flame photometry where involves the gravitational feeding of solution through a restricting capillary into an area of high velocity gas flow where small droplets are produced and passed into the flame

PREMIX BURNER

A component of Flame Photometer uses photocell as detector

DETECTOR

MONOCHROMATOR where transmit yellow light (589 nm)

Na Filter

INTERFERENCE FILTERS Where it transmit violet light (767 nm)

K FILTER

INTERFERENCE FILTERS where it transmit red light (761 nm)

LITHIUM

The Internal Standard in Flame Photometry

LITHIUM, CESIUM

The element is not excited in the flame but merely dissociated from its chemical bond and placed in an unexcited state. The atom, at a lower energy level, absorbs light. The light source emits radiant energy to be absorbed by the element.

ATOMIC ABSORPTION SPECTROPHOTOMETRY

Measures the amount of light absorbed by ground state atom

ATOMIC ABSORPTION SPECTROPHOTOMETRY

AAS Used to measure element symbol only

Al, Ca, Cu, Pb, Li, Mg, Zn

Components of Flame Photometer

GAS, BURNER ASSEMBLY, INTERFERENCE FILTER, DETECTOR

Components of AAS

LIGHT SOURCE, MECHANICAL ROTATING CHOPPER, BURNER, MONOCHROMATOR, DETECTOR, READ OUT DEVICE

Light Source in AAS which produces a wavelength of light specific for the kind of metal in the cathode

HOLLOW CATHODE LAMP

A component of AAS that modulates light beam coming from the hollow cathode lamp

MECHANICAL ROTATING CHOPPER

A component of AASuses flame to dissociate the chemical bonds and form free, unexcited atoms

BURNER

Type of Burner in AAS where flame is more concentrated and can be made hotter, thus lessening chemical interferences.

TOTAL CONSUMPTION BURNER

Type of Burner in AAS wheregases are mixed and the sample is atomized before entering the flame and the large droplets go to waste and not in the flame. It has less noisy signals with longer pathlength and greater absorption and sensitivity.

PRE MIX BURNER

A component of AAS selects the desired wavelength from a spectrum of wavelength which could either be a prism or a diffraction grating.

MONOCHROMATOR

A component of AAS uses photomultiplier tubes to measure the intensity of the light signal.

DETECTOR

energy emission that occurs when certain compounds absorb electromagnetic radiation, become excited and then return to an energy level that is usually slightly higher than their original level.

FLUORESCENCE

FLUORESCENCE Components

LIGHT SOURCE, MONOCHROMATOR, PHOTOMULTIPLIER, READ OUT DEVICE

100

Light Source of FLUORESCENCE SPECTROPHOTOMETRY

HYDROGEN DISCHARGE LAMP, XENON LAMP

COMPH Lesson 5

COMPH Lesson 5

73問 • 2年前

Jef Marc Valencia · 42問 · 2年前

Discussion 1

Discussion 1

42問 • 2年前

Jef Marc Valencia · 42問 · 2年前

Dis 1

Dis 1

42問 • 2年前

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Shift -Systematic Error

Shift -Systematic Error

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LEVEY – JENNINGS CHART

LEVEY – JENNINGS CHART

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WESTGARD MULTIRULE CHART

WESTGARD MULTIRULE CHART

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Random vs Systematic

Random vs Systematic

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Researcj Design

Researcj Design

48問 • 2年前

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Dis 1 mid

Dis 1 mid

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COMPM LESSON 6-7

COMPM LESSON 6-7

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Discussion -2 Six Sigma

Discussion -2 Six Sigma

31問 • 2年前

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Sigma Levels

Sigma Levels

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COMPH- LESSON 8

COMPH- LESSON 8

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Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

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WAVELENGTH Color Table

WAVELENGTH Color Table

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QUIz ANNOUNCEMENT part 2

QUIz ANNOUNCEMENT part 2

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Electrochem

Electrochem

62問 • 2年前

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ELEC TROCH

ELEC TROCH

18問 • 1年前

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TRANSCRIPTION

TRANSCRIPTION

49問 • 1年前

LIS

LIS

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Carbohydrates

Carbohydrates

16問 • 1年前

DIFFERENT PATHWAYS IN GLUCOSE METABOLISM

DIFFERENT PATHWAYS IN GLUCOSE METABOLISM

III. GLUCOSE MEASUREMENT

III. GLUCOSE MEASUREMENT

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Copper Reductions

Copper Reductions

7問 • 1年前

Exp.8

Exp.8

QUIZ 8/9

QUIZ 8/9

Immunology

Immunology

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Immunology part 2

Immunology part 2

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Immunology 3

Immunology 3

Lesson 4

Lesson 4

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PROTEINS

PROTEINS

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PROTEIN 2

PROTEIN 2

34問 • 1年前

PROTEIN 3

PROTEIN 3

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Serum Albumin Determination

Serum Albumin Determination

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CC lec debris

CC lec debris

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NPN

NPN

44問 • 1年前

NPN 2

NPN 2

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Materials management

Materials management

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Liver

Liver

57問 • 1年前

LESSON 10

LESSON 10

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hyperlipoproteinemia

hyperlipoproteinemia

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OBLIGATE INTRACELLULAR BACTERIA

OBLIGATE INTRACELLULAR BACTERIA

24問 • 1年前

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THE SPIROCHETES

THE SPIROCHETES

48問 • 1年前

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PYOGENIC GRAM POSTIVE

PYOGENIC GRAM POSTIVE

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Introduction

Introduction

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Introduction Page 2

Introduction Page 2

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Introduction pg 3

Introduction pg 3

31問 • 1年前

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Introduction table

Introduction table

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Introduction-body

Introduction-body

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NK-Immunity

NK-Immunity

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meet 2

meet 2

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discussion 3

discussion 3

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dis 1

dis 1

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Immuno vs anti

Immuno vs anti

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Immune 3

Immune 3

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dis 4

dis 4

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TRANSUDATIVE Vs. EXUDATIVE

TRANSUDATIVE Vs. EXUDATIVE

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dis 5

dis 5

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Preserved Tissue Examination Steps by definition

Preserved Tissue Examination Steps by definition

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FIXATIVES

FIXATIVES

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Long discussion 1

Long discussion 1

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ALDEHYDE-FIXATIVES

ALDEHYDE-FIXATIVES

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lactate dehydrogenase

lactate dehydrogenase

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FIXATIVES-METALLIC

FIXATIVES-METALLIC

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FIXATIVES-CHROMATE

FIXATIVES-CHROMATE

5問 • 1年前

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Specialized Macrophages

Specialized Macrophages

10問 • 1年前

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LYMPHOPOIESIS

LYMPHOPOIESIS

14問 • 1年前

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PICRIC ACID-FIXATIVE

PICRIC ACID-FIXATIVE

5問 • 1年前

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immune 4

immune 4

33問 • 1年前

FIXATIVES-ALCOHOL

FIXATIVES-ALCOHOL

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Immunoglobulins

Immunoglobulins

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ARTIFACTS

ARTIFACTS

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FIXATIVES QUIZ COPY

FIXATIVES QUIZ COPY

11問 • 1年前

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MAJOR HISTOCOMPATIBILITY

MAJOR HISTOCOMPATIBILITY

26問 • 1年前

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Introduction

Introduction

58問 • 1年前

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OTHERS-FIXATIVES

OTHERS-FIXATIVES

5問 • 1年前

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MEGAKARYOPOLESIS

MEGAKARYOPOLESIS

34問 • 1年前

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DECALCIFICATION

DECALCIFICATION

51問 • 1年前

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DEHYDRATION

DEHYDRATION

64問 • 1年前

Blood Specimen Collection

Blood Specimen Collection

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CLEARING

CLEARING

7問 • 1年前

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CEARING AGENT

CEARING AGENT

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INTRODUCTION

INTRODUCTION

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Jef Marc Valencia · 51問 · 1年前

Introduction

Introduction

51問 • 1年前

Jef Marc Valencia · 12問 · 1年前

Methods of Determination

Methods of Determination

12問 • 1年前

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ASPARTATE AMINOTRANSFERASE

ASPARTATE AMINOTRANSFERASE

11問 • 1年前

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ALANINE AMINOTRANSFERASE

ALANINE AMINOTRANSFERASE

15問 • 1年前

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Creatinine Kinase

Creatinine Kinase

34問 • 1年前

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LACTATE DEHYDROGENASE

LACTATE DEHYDROGENASE

27問 • 1年前

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ACID PHOSPHATASE

ACID PHOSPHATASE

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