NON-PROTEIN NITROGEN derived from the early days of _ where Analytic methodology required the_from a specimen before the analysis

the specimen of choice for the determination of urea

The concentration of nitrogen containing compound in PFF was

quantified spectrophotometrically converts_ to_ w/ to produce _

Diff. NPN compounds

Majority of NPN compounda arise from the _ of _and_

Highest concentration in the blood

Major excretory product of protein and AA catabolism

Urea Consist of % of the total non-protein nitrogen

Urea Approximately % of the total nitrogen that urea is excreted

Urea is Formed in the liver from _and _

Processes where Urea is formed

KREB’S HENSELEIT CYCLE is also known as

Since historic assays for urea were based on the measurement of nitrogen the term BUN has been used to refer

Protein metabolism produces_ that can be oxidized to produced _ or_ or stored as _

Process in Urea Pathyway of protein metabolism released _ which is converted in _ and excretes as a waste

Urea Concentration is dependent on

Waste product formed by the breakdown of amino acid/gut bacteria

2 stages of Urea Cycle

Dtermined what stage CP formed from Ammonia and bicarbonate by CPS

Dtermined what stage OTC condenses CP and Ornithine to form citrulline

Dtermined what stage Citrulline is transported to the cytosol by SLC25A15

Enzyme formed from ammonia and bicarbonate by CPS

Enyzme that condenses carbamoyl phosphate and ornithine to form_

Transported to the cytosol by

Dtermined what stage AS condenses citrulline and aspartate to form Argininosuccinate

Dtermined what stage Argininosuccinate is broken down into arginine and fumarate by AL

Dtermined what stage Arginine is broken down into Urea and ornithine by Arginase

Dtermined what stage Ornithine Translocase transports ornithine into the mitochondria

It condenses citrulline and aspartate to form argininosuccinate

Argininosuccinate is broken down into arginine and fumarate by

Arginine is broken down into _ and_by arginase

Transports ornithine into mitochondria

the first metabolite to elevate in kidney diseases and It is easily removed by

In stable nitrogen balance, _ of urea excreted daily

Specimen of choice in BUN abbrev

PFF is based on measuring amount of

Urea nitrogen concentration can be converted to urea concentration by multiplying by

SI unit Urea nitrogen concentration in mg/dL to mmol/L – multiply by

Specimen requirements of Urea

Specimen where it inhibit ammonium ions and high concentration of sodium citrate and SODIUM FLUORIDE must be avoided

Specimen of choice where fasting is not required

Specimen that cannot be analyzed within a few hours should be refrigerated as it is susceptible to bacterial infection

Specimen where it should be refrigerated during the collection period