MOUNTING AND LABELLING MCQ

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Refers to the water based mounting media

AQUEOUS

Refers to the water insoluble mounting media

NON AQUEOUS, RESINOUS

Process that involves the use of e medium and a coverslip to facilitate protection of the specimen from damage

MOUNTING

What is the refractive index of a good mounting media

1.518

Process of sealing the margins of the coverslip

RINGING

A type of ringing media which composed of 2 parts of paraffin +4-9 parts of powdered colophonium resin

KRONIG CEMENT

A type ringing media which composed of cellulose adhesive

DUROFIX

Higher refractive index is achieved by the use of high concentration of

SUGAR

A type of mounting media used for water miscible preparations directly form water (stain is removed w/ alcohol and xylene)

AQUEOUS

Aqueous mounting media are used for this type of specimen

LIPID

A type mounting media for preparation which were cleared using xylene

NON AQUEOUS

Mounting media commonly used for most staining method

NON AQUEOUS

Resinous mounting media have a refractive index of greater or equal to

1.518

What is the refractive index of Aqueous mounting media

1.40

Added to mountants to the point of saturation in order to reduce the 'bleeding' of cationic dyes at the same time giving a pH of approximately 7.0

POTASSIUM ACETATE

Used as temporary mounting medium which evaporates easily and not suitable for OIO, give also the refractive index

WATER, 1.33

Aqueous mounting media used as preservative, sets hard and keep sections for years after ringing.

GLYCERIN

An aqueous standard mountants when dehydration and clearing w/ xylene are not performed. Give also the refractive index

GLYCERIN JELLY, 1.47

It is made of gelatin+glycerol+distilled water+phenol crystals . It stain tends to face and does not set solidify on storage

GLYCERIN JELLY

What is the refractive index of a good mounting media

1.518

A good mountant should have a refiective index close to the glass and tissue to prevent excess bending of light in microscopy.

TRUE

When excess xylene is mixed with the mountant the tissue will be more miscible to the mountant.

FALSE

Canada balsam has been widely used for over 150 years due to its archival quality.

TRUE

Excess mounting media will improperly set the coverslip and form bubbles.

FALSE

Once the tissue is mounted it cannot be remounted anymore.

FALSE

A-good ringing media should not evaporate the mounting media.

TRUE

Durofix ringing media is great for tissues embedded in resin.

FALSE

Canada balsam is primarily dissolved in water for preparation.

FALSE

Aqueous mounting media require the addition of bacteriostatic agents to prevent microbial growth.

TRUE

The refractive index of glycerin jelly is 1.33

FALSE

A good mounting medium should shrink back from the edge of the cover glass to ensure specimen stabi

FALSE

Apathy's medium is a non-fluorescent aqueous mounting medium useful for fluorescent microscopy.

TRUE

Synthetic resin mountants like DPX have a higher refractive index than Canada balsamn.

TRUE

Mounting media affects the imaging quality by influencing the transparency of tissue sections

TRUE

Air bubblés under the coverslip can always be removed by applying pressure.

FALSE

IHC is used for assessing metastasis using dye-based interactions

FALSE

The direct method in IHC is less sensitive because the signal or color is too weak.

TRUE

The disadvantage of IHC is it could not detect micro tumors that are spreading beyond the area of origin of the cancer

FALSE

Immunohistochemistry has revolutionized the classification of tumors of uncertain origin by providing specific antigen localization.

TRUE

Immunohistochemistry is rore effective than microbial culture for detecting dificult-to-grow pathogens in tissue samples.

TRUE

IHC provides no advantage over traditional histological techniques in diagnosing degenerative brain diseases.

FALSE

IHC can differentiate between morphologically similar tumors by identifying distinct antigens

TRUE

The direct method of IHC involves two steps antigen binding and signal amplification by secondary antibodies.

FALSE

The use of lHC in surgical pathology has significantly improved the accuracy of tumor staging and treatment decisions.

TRUE

The tissue must be dried first after staining then continue to mounting

FALSE

Germ cell marker

ALPHA FETOPROTEIN

Intermediate filament marker

EPITHELIAL TUMOR MARKER

Neuroendocrine marker

CARCINOEMBRYONIC ANTIGEN

MESENCHYMAL TUMOR MARKER

LEUKOCYTE COMMON ANTGEN

Epithelial tumor marker

PROSTATE SPECIFIC ANTIGEN

COMPH Lesson 5

COMPH Lesson 5

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Discussion 1

Discussion 1

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Dis 1

Dis 1

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Shift -Systematic Error

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LEVEY – JENNINGS CHART

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WESTGARD MULTIRULE CHART

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Random vs Systematic

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Researcj Design

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Dis 1 mid

Dis 1 mid

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COMPM LESSON 6-7

COMPM LESSON 6-7

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Sigma Levels

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COMPH- LESSON 8

COMPH- LESSON 8

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Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

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WAVELENGTH Color Table

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ELEC TROCH

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TRANSCRIPTION

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LIS

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Carbohydrates

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Exp.8

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QUIZ 8/9

QUIZ 8/9

Immunology

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Immunology part 2

Immunology part 2

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Immunology 3

Immunology 3

Lesson 4

Lesson 4

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PROTEINS

PROTEINS

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PROTEIN 2

PROTEIN 2

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PROTEIN 3

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CC lec debris

CC lec debris

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NPN

NPN

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NPN 2

NPN 2

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Materials management

Materials management

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Liver

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LESSON 10

LESSON 10

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hyperlipoproteinemia

hyperlipoproteinemia

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OBLIGATE INTRACELLULAR BACTERIA

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THE SPIROCHETES

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PYOGENIC GRAM POSTIVE

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Introduction

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Introduction Page 2

Introduction Page 2

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Introduction pg 3

Introduction pg 3

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Introduction table

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Introduction-body

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NK-Immunity

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meet 2

meet 2

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discussion 3

discussion 3

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dis 1

dis 1

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Immuno vs anti

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Immune 3

Immune 3

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dis 4

dis 4

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TRANSUDATIVE Vs. EXUDATIVE

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dis 5

dis 5

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Preserved Tissue Examination Steps by definition

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FIXATIVES

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Long discussion 1

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ALDEHYDE-FIXATIVES

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lactate dehydrogenase

lactate dehydrogenase

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FIXATIVES-METALLIC

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FIXATIVES-CHROMATE

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Specialized Macrophages

Specialized Macrophages

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LYMPHOPOIESIS

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immune 4

immune 4

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Immunoglobulins

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ARTIFACTS

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FIXATIVES QUIZ COPY

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MAJOR HISTOCOMPATIBILITY

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Introduction

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OTHERS-FIXATIVES

OTHERS-FIXATIVES

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MEGAKARYOPOLESIS

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DEHYDRATION

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Blood Specimen Collection

Blood Specimen Collection

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CLEARING

CLEARING

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CEARING AGENT

CEARING AGENT

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INTRODUCTION

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Introduction

Introduction

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Methods of Determination

Methods of Determination

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ASPARTATE AMINOTRANSFERASE

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ALANINE AMINOTRANSFERASE

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Creatinine Kinase

Creatinine Kinase

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LACTATE DEHYDROGENASE

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ACID PHOSPHATASE

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