HEMOCYTOMETRY

83問 • 5ヶ月前

問題一覧

Device used to perform visual blood and body fluid cell counts, consisting of a microscopic slide w/ a depression whose polished glass base is marked w/ grids and into which a measured volume of a sample of diluted blood is placed

HEMOCYTOMETER, HEMACYTOMETER, COUNTING CHAMBER

Cmonly used counter for Blood cell counting

TALLY COUNTER

Numerical evaluation of formed elements of blood and the estimation of the number of blood cells in a known volume of blood

HEMOCYTOMETRY

Unit used to in relative cell count reporting

PERCENTAGE

Unit used in absolute cell counting for RBC

X 10^12/L

Unit used in absolute cell counting for WBC and Platelets

X 10^9/L

Microglass capillary pipets that suck in just the right amount of sample connected to a plastic container containing just th eright amount of sample

AUTOMATIC PIPET

Examples of automatic pipets

TRENNER, UNOPETTE

Most c'monly used automatic pipet

UNOPETTE

These are the most c'monly used diluting pipets for blood cell counting

NON AUTOMATIC PIPET

Example of non automatic pipets

THOMA PIPET

Used to pierce the Resevoir of unopette

PIPETTE SHIELD

This cointains the diluent of Unopette

RESERVOIR

Red bead

RBC PIPET

White bead

WBC PIPET

Larger Bulb size

RBC PIPET

Smaller Bulb size

WBC PIPET

100 bulb volume

RBC PIPET

10 bulb volume

WBC PIPET

Smaller bore size

RBC PIPET

Larger bore size

WBC PIPET

Calibrations are 0.5, 1, 101

RBC PIPET

Calibrations are 0.5, 1, 11

WBC PIPET

Usual dilution of 1:200

RBC PIPET

Usual dilution of 1:20

WBC PIPET

Dilution range of 1:100-1:1000

RBC PIPET

Dilution range of 1:10-1:100

WBC PIPET

Recommended dilution and pipet to be used if the anticipated WBC count is 0.1-3.0 x10^9/L

1:10, WBC

Recommended dilution and pipet to be used if the anticipated WBC count is 3.1-30.0 x10^9/L

1:20, WBC

Recommended dilution and pipet to be used if the anticipated WBC count is >30.0 x10^9/L

1:100, RBC

Recommended dilution and pipet to be used if the anticipated WBC count is >=100.0 x10^9/L

1:200, RBC

Conditions that may caused leukocytopenia

LEUKEMIA, INFECTION

Conditions that may caused WBC count to beyond 100.0 x 10^9/L

POLYCYTHEMIA VERA, LEUKEMIA

Open Types of Hemocytometer

SPENCER, LEVY, LEVY HAUSER

Closed type of Hemocytometer

THOMA-ZEIS

Types of hemocytometer according to rulings

THOMA, TUERK, BASS JONE, IMPROVED NEUBAUER, FUCHS ROSENTHAL

Used for counting cells in the CSF and Exclusively eosinophils and basophils

FUCHS ROSENTHAL

Dimensions of Fuchs Rosenthal Depth: Number of ruled areas: Width and length of each ruled area: Number of square each ruled area:

0.2MM, 2, 4MM X 4MM, 16

Dimensions of Speirs Levy Depth: Number of ruled areas: Width and length of each ruled area: Number of square each ruled area:

0.2MM, 4, 2MM X 5MM, 10

Dimensions of Improved Neubauer Depth: Number of ruled areas: Width and length of each ruled area: Number of square each ruled area:

0.1MM, 2, 3MM X 3MM, 9

What coverslip should be used for cell counting

NON DISPOSABLE

How manty RBC small squared is used for RBC cell counting

How many squares are used for WBC cell counting

How many squares is used for Unopette for WBC counting

How many small squares cointaing in one WBC square

Dimensions of 1 Large WBC square Length and Width: Area: Volume:

1MM X 1MM, 1MM2, 0.1MM3

Dimensions of 1 small WBC square Length and Width: Area: Volume:

0.25MM X 0.25MM, 0.0625MM2, 0.00625MM3

How many small RBC square does central square has

Dimensions of 1 Large RBC square Length and Width: Area: Volume:

0.2MM X 0.2MM, 0.04MM2, 0.004MM3

Dimensions of 1 small RBC square Length and Width: Area: Volume:

0.05MM X 0.05MM, 0.0025MM2, 0.00025MM3

Angle for Neubauer charging

Objective lens used for WBC counting

LPO

Objective lens used for RBC counting

HPO

Cell counting has and inverted L rule which pertains to counting cells that sits in the border of the square excluding what border

BOTTOM, RIGHT

This causes difficulty in distinguishing the cells

DUST, FINGERPRINT

If the count is low, a ___are may be counted to improve accuracy

GREATER

WBC diluting fluids

2-3% GLACIAL ACETIC ACID, 1% HYDROCHLORIC ACID, TURKS SOLUTION

Components of Turks solution

3ML GLACIAL ACETIC ACID, 96ML DISTILLED WATER, 1ML 1% AQEUOUS GENTIAN VIOLET

RBC diluting fluid that initiates mold formation and rouleaux formation that can stand for a long time and no corrosive effect

HAYEMS

RBC diluting fluid that prevents rouleaux formation and precipitation or CHONS

GOWERS

RBC diluting fluid that initiates mold formation and has high specific gravity and w/ stain

TOISSONS

Best RBC diluting fluid that has preservative action and prevents mold formation that does not alter cell morphology

FORMOL CITRATE, DACIES FLUID

Components of Dacies fluid

10ML 50% FORMALIN, 990ML 3% SODIUM CITRATE

RBC diluting fluid used in cases of emergency when no other RBC diluting fluids are available ideally to use in cases of excessive rouleaux and autoagglutination

0.85-0.90% NORMAL SALINE SOLUTION

RBC diluting fluid used for platelet counting and coagulation studies

3.8% SODIUM CITRATE

Composition of Bethels RBC diluting fluid

SODIUM CHLORIDE, SODIUM SULFATE, GLYCERIN, DISTILLED WATER, SODIUM MERTHIOLATE

Composition of 0.85%-0.90% NSS

SODIUM CHLORIDE, DISTILLED WATER

Normal value for RBC male

4.5-5.9 X 10^12/L

Normal value for RBC female

4.1-5.1 X 10^12/L

Normal WBC count

4.4-11.3 X 10^9/L

Normal value for Platelet count

150-450 X 10^9/L

Diluting fluid ideal characterictics

CHEAP, PRESERVATIVE ACTION, HIGH SPECIFIC GRAVITY, BUFFER ACTION, NON ALLERGIC/CORROSIVE

Tonicity recommended for RBCs

ISOTONIC

Tonicity recommended for WBC

HYPOTONIC

States that cells settles at random by allowing cells to settle by letting it stand for 3 mins

POISSONS LAW OF DISTRIBUTION

Difference of 2 chambers requiring recharge

>2SD

Difference of 2 chambers requiring to get the average

<2SD

There should be no more than __cell difference between the highest and lowest number of WBCs

There should be no more than __cell difference between the highest and lowest number of RBCs

WBC pipet accuracy

+/-3%

RBC pipet accuracy

+/-5%

Errors in manual hemocytometry

TECHNICAL/HUMAN, EQUIPMENT/STANDARD, INHERENT/FIELD ERROR

Test other than RBC counting that provides estimates for RBC count

MEAN CORPUSCULAR VOLUME, HEMATOCRIT

COMPH Lesson 5

Jef Marc Valencia · 73問 · 2年前

COMPH Lesson 5