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HEMOCYTOMETRY

  • Jef Marc Valencia

    • 問題数 83 • 10/3/2025

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  • 1

    Device used to perform visual blood and body fluid cell counts, consisting of a microscopic slide w/ a depression whose polished glass base is marked w/ grids and into which a measured volume of a sample of diluted blood is placed

    HEMOCYTOMETER, HEMACYTOMETER, COUNTING CHAMBER

  • 2

    Cmonly used counter for Blood cell counting

    TALLY COUNTER

  • 3

    Numerical evaluation of formed elements of blood and the estimation of the number of blood cells in a known volume of blood

    HEMOCYTOMETRY

  • 4

    Unit used to in relative cell count reporting

    PERCENTAGE

  • 5

    Unit used in absolute cell counting for RBC

    X 10^12/L

  • 6

    Unit used in absolute cell counting for WBC and Platelets

    X 10^9/L

  • 7

    Microglass capillary pipets that suck in just the right amount of sample connected to a plastic container containing just th eright amount of sample

    AUTOMATIC PIPET

  • 8

    Examples of automatic pipets

    TRENNER, UNOPETTE

  • 9

    Most c'monly used automatic pipet

    UNOPETTE

  • 10

    These are the most c'monly used diluting pipets for blood cell counting

    NON AUTOMATIC PIPET

  • 11

    Example of non automatic pipets

    THOMA PIPET

  • 12

    Used to pierce the Resevoir of unopette

    PIPETTE SHIELD

  • 13

    This cointains the diluent of Unopette

    RESERVOIR

  • 14

    Red bead

    RBC PIPET

  • 15

    White bead

    WBC PIPET

  • 16

    Larger Bulb size

    RBC PIPET

  • 17

    Smaller Bulb size

    WBC PIPET

  • 18

    100 bulb volume

    RBC PIPET

  • 19

    10 bulb volume

    WBC PIPET

  • 20

    Smaller bore size

    RBC PIPET

  • 21

    Larger bore size

    WBC PIPET

  • 22

    Calibrations are 0.5, 1, 101

    RBC PIPET

  • 23

    Calibrations are 0.5, 1, 11

    WBC PIPET

  • 24

    Usual dilution of 1:200

    RBC PIPET

  • 25

    Usual dilution of 1:20

    WBC PIPET

  • 26

    Dilution range of 1:100-1:1000

    RBC PIPET

  • 27

    Dilution range of 1:10-1:100

    WBC PIPET

  • 28

    Recommended dilution and pipet to be used if the anticipated WBC count is 0.1-3.0 x10^9/L

    1:10, WBC

  • 29

    Recommended dilution and pipet to be used if the anticipated WBC count is 3.1-30.0 x10^9/L

    1:20, WBC

  • 30

    Recommended dilution and pipet to be used if the anticipated WBC count is >30.0 x10^9/L

    1:100, RBC

  • 31

    Recommended dilution and pipet to be used if the anticipated WBC count is >=100.0 x10^9/L

    1:200, RBC

  • 32

    Conditions that may caused leukocytopenia

    LEUKEMIA, INFECTION

  • 33

    Conditions that may caused WBC count to beyond 100.0 x 10^9/L

    POLYCYTHEMIA VERA, LEUKEMIA

  • 34

    Open Types of Hemocytometer

    SPENCER, LEVY, LEVY HAUSER

  • 35

    Closed type of Hemocytometer

    THOMA-ZEIS

  • 36

    Types of hemocytometer according to rulings

    THOMA, TUERK, BASS JONE, IMPROVED NEUBAUER, FUCHS ROSENTHAL

  • 37

    Used for counting cells in the CSF and Exclusively eosinophils and basophils

    FUCHS ROSENTHAL

  • 38

    Dimensions of Fuchs Rosenthal Depth: Number of ruled areas: Width and length of each ruled area: Number of square each ruled area:

    0.2MM, 2, 4MM X 4MM, 16

  • 39

    Dimensions of Speirs Levy Depth: Number of ruled areas: Width and length of each ruled area: Number of square each ruled area:

    0.2MM, 4, 2MM X 5MM, 10

  • 40

    Dimensions of Improved Neubauer Depth: Number of ruled areas: Width and length of each ruled area: Number of square each ruled area:

    0.1MM, 2, 3MM X 3MM, 9

  • 41

    What coverslip should be used for cell counting

    NON DISPOSABLE

  • 42

    How manty RBC small squared is used for RBC cell counting

    5

  • 43

    How many squares are used for WBC cell counting

    4

  • 44

    How many squares is used for Unopette for WBC counting

    9

  • 45

    How many small squares cointaing in one WBC square

    16

  • 46

    Dimensions of 1 Large WBC square Length and Width: Area: Volume:

    1MM X 1MM, 1MM2, 0.1MM3

  • 47

    Dimensions of 1 small WBC square Length and Width: Area: Volume:

    0.25MM X 0.25MM, 0.0625MM2, 0.00625MM3

  • 48

    How many small RBC square does central square has

    25

  • 49

    Dimensions of 1 Large RBC square Length and Width: Area: Volume:

    0.2MM X 0.2MM, 0.04MM2, 0.004MM3

  • 50

    Dimensions of 1 small RBC square Length and Width: Area: Volume:

    0.05MM X 0.05MM, 0.0025MM2, 0.00025MM3

  • 51

    Angle for Neubauer charging

    45

  • 52

    Objective lens used for WBC counting

    LPO

  • 53

    Objective lens used for RBC counting

    HPO

  • 54

    Cell counting has and inverted L rule which pertains to counting cells that sits in the border of the square excluding what border

    BOTTOM, RIGHT

  • 55

    This causes difficulty in distinguishing the cells

    DUST, FINGERPRINT

  • 56

    If the count is low, a ___are may be counted to improve accuracy

    GREATER

  • 57

    WBC diluting fluids

    2-3% GLACIAL ACETIC ACID, 1% HYDROCHLORIC ACID, TURKS SOLUTION

  • 58

    Components of Turks solution

    3ML GLACIAL ACETIC ACID, 96ML DISTILLED WATER, 1ML 1% AQEUOUS GENTIAN VIOLET

  • 59

    RBC diluting fluid that initiates mold formation and rouleaux formation that can stand for a long time and no corrosive effect

    HAYEMS

  • 60

    RBC diluting fluid that prevents rouleaux formation and precipitation or CHONS

    GOWERS

  • 61

    RBC diluting fluid that initiates mold formation and has high specific gravity and w/ stain

    TOISSONS

  • 62

    Best RBC diluting fluid that has preservative action and prevents mold formation that does not alter cell morphology

    FORMOL CITRATE, DACIES FLUID

  • 63

    Components of Dacies fluid

    10ML 50% FORMALIN, 990ML 3% SODIUM CITRATE

  • 64

    RBC diluting fluid used in cases of emergency when no other RBC diluting fluids are available ideally to use in cases of excessive rouleaux and autoagglutination

    0.85-0.90% NORMAL SALINE SOLUTION

  • 65

    RBC diluting fluid used for platelet counting and coagulation studies

    3.8% SODIUM CITRATE

  • 66

    Composition of Bethels RBC diluting fluid

    SODIUM CHLORIDE, SODIUM SULFATE, GLYCERIN, DISTILLED WATER, SODIUM MERTHIOLATE

  • 67

    Composition of 0.85%-0.90% NSS

    SODIUM CHLORIDE, DISTILLED WATER

  • 68

    Normal value for RBC male

    4.5-5.9 X 10^12/L

  • 69

    Normal value for RBC female

    4.1-5.1 X 10^12/L

  • 70

    Normal WBC count

    4.4-11.3 X 10^9/L

  • 71

    Normal value for Platelet count

    150-450 X 10^9/L

  • 72

    Diluting fluid ideal characterictics

    CHEAP, PRESERVATIVE ACTION, HIGH SPECIFIC GRAVITY, BUFFER ACTION, NON ALLERGIC/CORROSIVE

  • 73

    Tonicity recommended for RBCs

    ISOTONIC

  • 74

    Tonicity recommended for WBC

    HYPOTONIC

  • 75

    States that cells settles at random by allowing cells to settle by letting it stand for 3 mins

    POISSONS LAW OF DISTRIBUTION

  • 76

    Difference of 2 chambers requiring recharge

    >2SD

  • 77

    Difference of 2 chambers requiring to get the average

    <2SD

  • 78

    There should be no more than __cell difference between the highest and lowest number of WBCs

    15

  • 79

    There should be no more than __cell difference between the highest and lowest number of RBCs

    20

  • 80

    WBC pipet accuracy

    +/-3%

  • 81

    RBC pipet accuracy

    +/-5%

  • 82

    Errors in manual hemocytometry

    TECHNICAL/HUMAN, EQUIPMENT/STANDARD, INHERENT/FIELD ERROR

  • 83

    Test other than RBC counting that provides estimates for RBC count

    MEAN CORPUSCULAR VOLUME, HEMATOCRIT

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    SPERM

    問題数 632025/04/22

    SPERM 2

    問題数 692025/04/22

    Fructose test table

    問題数 52025/04/22

    Sperm motility table

    問題数 82025/04/22

    PROTOZOAN

    問題数 812025/04/24

    CSF

    問題数 962025/04/29

    AMOEBA

    問題数 222025/04/29

    MCQ-CHEMICAL EXAMINATION OF URINE PART 2

    問題数 792025/05/03

    PERICARDIAL FLUID

    問題数 662025/05/04

    MCQ-SPERM

    問題数 592025/05/03

    MCQ-SPERM 2

    問題数 882025/05/03

    MCQ-AMNIOTIC FLUID

    問題数 732025/05/08

    HEMOFLAGELLATES

    問題数 682025/05/04

    Sex disorder

    問題数 602025/05/05

    NEMATODES

    問題数 62025/05/06

    MCQ-PERICARDIAL FLUID

    問題数 592025/05/11

    PHASMID NEMATODES

    問題数 52025/05/06

    HOOKWORMS

    問題数 92025/05/06

    MCQ-CSF

    問題数 942025/05/03

    BLOOD AND TISSUE NEMATODE

    問題数 82025/05/06

    PLATYHELMINTHS

    問題数 132025/05/06

    MCQ-CSF2

    問題数 242025/05/03

    TREMATODES

    問題数 72025/05/06

    INTRODUCTION

    問題数 362025/06/10

    INTRODUCTION TABLE

    問題数 412025/06/10

    CLASSFICATION OF ENZYME

    問題数 472025/06/11

    ENZYME VARIANTS

    問題数 412025/06/11

    FACTORS AFFECTING ENZYME ACTIVITY

    問題数 902025/06/12

    INTRODUCTION TO ENZYMOLOGY-MCQ

    問題数 412025/06/26

    CLASS AND VARIANT OF ENZYME-MCQ

    問題数 772025/06/26

    PITUITARY GLAND PART 2

    問題数 52025/07/04

    THYROID GLAND

    問題数 712025/07/04

    PARATHYROID GLAND

    問題数 122025/07/04

    ADRENAL GLAND

    問題数 772025/07/04

    SEX GLANDS

    問題数 352025/07/04

    OTHER GLANDS

    問題数 82025/07/04

    ACID BASE BALANCE

    問題数 512025/07/10

    TRACE ELEMENT

    問題数 792025/07/10

    VITAMINS

    問題数 272025/07/10

    TUMOR MARKERS

    問題数 252025/07/10

    TOXICOLOGY

    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26