Hemoglobin Determination

25問 • 1年前

問題一覧

It is the hemoglobin determination which based on the principle of a given blood sample can be equilibrated with oxygen under standard conditions of temperature and pressure.

GASOMETRIC

It is the hemoglobin determination which based on the principle of total iron content of the blood

CHEMICAL

It is the hemoglobin determination which uses copper sulfate solution

GRAVIMETRIC

How many milligrams of iron contains in one gm of hemoglobin

3.47

Method in which iron is made to form ferricthiocyanate which is then measured spectrophotometrically

WONG'S METHOD

How many seconds after the drop of that becomes quoted with cover proteinate when exposed to copper sulfate solution

What is standard specific gravity of copper sulfate in blood mass donation

1.053

What is the reference value of specific gravity of hemoglobin determination in female

1.053, 12.5 g/dL

What is the reference value of specific gravity of hemoglobin determination in male

1.055, 13.5 g/dL

Which of these direct visual colorimetric base on the principle of patients and diluted blood is absorbed onto an absorbent pad and the color is compared with litograph color scale representing values from 10 to 100%

TALLQUIST METHOD

Which of these direct visual colorimetric base on the principle of blood is drawn by capillary action between two glass plates and the color of blood is much with the rotating dicks of red tinted glass with varying thickness and color

DARES HEMOGLOBINOMETER

Which of these is acid hematin method

SAHLI-HAYDEN

Which of these is alkali hematin method

KLEGG AND KING

A photoelectric colorometric method which a 0.02 ml of whole blood is reacted with 5 ml of 0.07 N nh4oh or 0.1% and na2co3 then mix for proper oxygenation and absorbance is read at 540 nm against reagent blank

OXYHEMOGLOBIN

A method in oxyhemoglobin in which absorbance is read at 415 nm or soret band which is about 400 to 430 nm

HARBOE

A method in which hemoglobin is catalyzes the rapid oxidation of benzidine by h2o2 and it is more sensitive but less accurate

NAUMANN

It is the most accurate and considered to be the gold standard in hemoglobin determination in which the principle is the blood is mixed with a solution of potassium cyanide and potassium ferracyanide erythrocytes are lysed thereby producing hemoglobin potassium ferricyanide oxidizes hemoglobin into methemoglobin that combines with potassium cyanide to produce hemoglobin cyanide whose absorbance is measured at 540 against reagent blank

CYANMETHEMOGLOBIN

The abnormal hemoglobin in which the cyanmethemoglobin cannot be transformed

SULFHEMOGLOBIN

This is the original drabkins reagent which is used in cyanmethemoglobin method

SODIUM BICARBONATE

What is the ph of drabkins reagent

7.0-7.4

A quantitation of the percentage of hbf in the blood in which hemolysis is organized and the neutralized and the denatured adult hp is precipitated by ammonium sulfate. Filtrate well then contain only alkali resistant hemoglobin.

ALKALI DENATURATION, BETKE METHOD, SINGER METHOD

It is used to assess whether the distribution of hbf in all red cell is the same and it uses the principle of hemoglobins other than hbf are related from the red cells

ACID ELUTION, MODIFICATION OF KLEIHAUER AND BETKE BY SHEPARD

It is used for the variant identifications of hemoglobin

HEMOGLOBIN ELECTROPHORESIS

It is used for the detection and preliminary identification of both normal and abnormal hemoglobins particularly hb safs and c

CELLULOSE ACETATE ELECTROPHORESIS

It is used for the separation of hps that migrate together on cellulose acetate also useful in detecting small amounts of either hba or f in the sequence or large amounts of the others and in revealing small amounts of adult hbs ans present at birth in cord blood

CITRATE AGAR ELECTROPHORESIS

COMPH Lesson 5

Jef Marc Valencia · 73問 · 2年前

COMPH Lesson 5