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Infiltrating and embedding media

  • Jef Marc Valencia

    • 問題数 62 • 9/27/2024

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    問題一覧

  • 1

    It is the simplest most common and best embedding medium which doesn't distort even it is immersed not more than 4 hours or use in serial sectioning

    PARAFFIN WAX

  • 2

    What is the most common melting point of paraffin wax

    56-58

  • 3

    Paraffin wax needed to be pure and filtered before use, what kind of filter should be used

    COARSE FILTER PAPER

  • 4

    How many times the maximum reuse of paraffin wax

    2

  • 5

    If paraffin wax was heated to about 100 to 105° c what substance or element trying to be removed

    WATER

  • 6

    Paraffin wax should not be used with this kind of tissues as it can melt it out

    FATTY TISSUE

  • 7

    If fixed knife microtome is used what kind of paraffin wax should be used

    HIGH MELTING POINT

  • 8

    What is added on the paraffin to become paraplast

    SYNTHETIC PLASTIC POLYMER

  • 9

    Paraplast has a melting point of what temperature

    56-57

  • 10

    This is good for bones and brain and better in ribboning sections as it does not deposits on the slide after staining and requires no station processing schedule

    PARAPLAST

  • 11

    This is the substitute similar with paraplast which has the same melting point which is less brittle and less compressible than paraplast

    EMBEDDOL

  • 12

    This is used for embedding the eyes

    BIOLOID

  • 13

    Same as paraplast but it contains rubber

    TISSUE MAT

  • 14

    It has low melting point but it is harder than paraffin and it is not soluble in water but only in 95% ethanol and clearing agents

    ESTER WAX

  • 15

    This medium requires a heavy duty microtome for sectioning

    ESTER WAX

  • 16

    This medium is used for enzyme histochemical studies and demonstration of neutral and lipids but it is hydroscopic which attracts water

    CARBOWAX

  • 17

    Carbowax requires how many changes

    4

  • 18

    What solution do we use in floating tissue ribbons

    PEARSE SOLUTION, BLANK AND MCCARTHY SOLUTION

  • 19

    The most common adhesive for ribbon to be adhered on the slide

    EGG ALBUMIN

  • 20

    This is added to the water for floatation when carbowax is used

    SOAP, 10% POLYETHYLENE GLYCOL

  • 21

    Celloidin is a purified form of what compound

    NITROCELLULOSE

  • 22

    This medium is used for hollow cavities which tend to collapse like eyes and this is also used for hard tissues like bones and teeth and can also be used in whole embryo

    CELLOIDIN

  • 23

    What are the percentages requirement for celloidin according to the thickness of the following tissues Thin- Medium- Thick-

    2-4, 4-6, 8-12

  • 24

    This medium can be used for neurological tissues and it reduces distortion in sectioning due to its robbery consistency it also requires no heat

    CELLOIDIN

  • 25

    This medium is very volatile and should be stored in 70 to 80% alcohol. Photo micrographs is also difficult to prepare in this medium

    CELLOIDIN

  • 26

    This is highly flammable and it used in gun cotton and gunpowder as propellant for firearms

    NITROCELLULOSE

  • 27

    What method of celloidine impregnation for bones teeth and brain and whole organs which requires soaking in 70 to 80% alcohol until ready for cutting

    WET

  • 28

    What method in celloidine impregnation which is for wholel eyes and requires gilson's mixture which is chloroform plus cedar wood oil but does not need to be stored in alcohol

    DRY

  • 29

    This medium can able to tolerate water and more easily to torn than celloidin however it is more explosive than celloidin. It would explode if hit even dry and exposure to direct sunlight should be avoided. This requires special equipment and non-conventional microtomy techniques which is not suitable for clinical histology laboratories

    LOW VISCOSITY NITROCELLULOSE

  • 30

    This medium is used for light microscopic studies and for high resolution microscopy of tissues that sections which requires thinner than usual about 4 to 6 um and can be used also for renal biopsies and bone marrow biopsies

    PLASTIC RESIN

  • 31

    Classification of plastic resins according to their chemical composition

    EPOXY, POLYESTER, ACRYLIC

  • 32

    This is use for ultra structural studies and permits section as thin as 30 to 40 mm to be cut since it is stable in an electron beam

    EPOXY

  • 33

    This reduces antigenicity but it is but it is sensitive on skin contact and inhalation

    EPOXY

  • 34

    This is the toxic component of epoxy

    VINYLCYCLOHEXANE DIOXIDE

  • 35

    This epoxy is the slowest infiltration

    BISPHENOL A

  • 36

    This epoxy is the fastest infiltration

    CYCLOHEXENE DIOXIDE

  • 37

    This type of plastic resin medium is rarely used and but it is used for em however epoxide are more superior

    POLYESTER

  • 38

    It is a type of acrylic plastic resin medium used for light microscopy due to hydrophilic nature

    POLYGLYCOL METHACRYLATE

  • 39

    It is a type of acrylic plastic racine used for undicalsified bone and other hard tissues due to hardness

    METHYL METHACRYLATE

  • 40

    In LVN medium, it needs these plasticisers to prevent tendency of tissues to crack

    OLEUM RACINI, CASTOR OIL

  • 41

    Medium used when dehydration is avoided or when tissues is subjected to histochemical and enzyme studies

    GELATIN IMPREGNATION

  • 42

    It is a medium used for delicate specimens like friable, necrotic tissues and frozen tissues sections

    GELATIN IMPREGNATION

  • 43

    In gelatin impregnation, the whole organs are subject to what kind of technique

    GOUGH WENWORTH TECHNIQUE

  • 44

    When washing out the fixatives using the gelatin infiltration, the tissues are placed with these concentrations

    20% GELATIN, 1% PHENOL

  • 45

    This prevents the growth of mold in gelatin infiltration

    1% PHENOL

  • 46

    Preferred fixative for gelatin infiltration for 12-24 hours

    10% FORMALIN

  • 47

    What is the preferred tissue width in mm and volume of gelatin infiltration

    2-3, 25X

  • 48

    It is mainly used in double embedding as this medium do not prove sufficient support. They are used for FNAB specimens and small friable pieces

    AGAR IMPREGNATION

  • 49

    The immortalisation of tissues presentation

    EMBEDDING

  • 50

    Minimum allowance of surrounding margin of wax

    2

  • 51

    This is the marker used and putted on the side of the tissue opposite that to be cut during grossing

    INDIA INK

  • 52

    This must always be read during embedding for proper orientation

    REQUISITION FORM

  • 53

    What ink should be used on margins of the tissue

    INDIA INK

  • 54

    These type of tissues are placed diagonally

    ELONGATED

  • 55

    Tubular tissues like the arteries, vein, fallopian tubes and GITs should be embedded in what perspective

    CROSS SECTION

  • 56

    Skin biopsies like shave and punch must be oriented in what perspective

    SIDE

  • 57

    Multiple tissues pieces are aligned in what perspective in the center of the mold

    ACROSS THE LONG AXIS

  • 58

    Membrane specimens should be oriented in what perspective

    SWISS ROLE

  • 59

    Endometrial curettings specimen should be oriented in what perspective

    KEEP IN CENTER

  • 60

    An embedding mold with an L shaped metal plate

    LEUCKHART'S EMBEDDING MOLD

  • 61

    The methods wherein tissues are first infiltrated w/CELLOIDIN and subsequently embedded in paraffin mass. This is used to facilitate cutting of large blocks of dense firm tissues like brain recommended for making small sections of celloidin blocks

    DOUBLE EMBEDDING

  • 62

    The process of removing paraffin to form a 4 sided prism

    TRIMMING

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    INTRODUCTION

    問題数 362025/06/10

    INTRODUCTION TABLE

    問題数 412025/06/10

    CLASSFICATION OF ENZYME

    問題数 472025/06/11

    ENZYME VARIANTS

    問題数 412025/06/11

    FACTORS AFFECTING ENZYME ACTIVITY

    問題数 902025/06/12

    INTRODUCTION TO ENZYMOLOGY-MCQ

    問題数 412025/06/26

    CLASS AND VARIANT OF ENZYME-MCQ

    問題数 772025/06/26

    PITUITARY GLAND PART 2

    問題数 52025/07/04

    THYROID GLAND

    問題数 712025/07/04

    PARATHYROID GLAND

    問題数 122025/07/04

    ADRENAL GLAND

    問題数 772025/07/04

    SEX GLANDS

    問題数 352025/07/04

    OTHER GLANDS

    問題数 82025/07/04

    ACID BASE BALANCE

    問題数 512025/07/10

    TRACE ELEMENT

    問題数 792025/07/10

    VITAMINS

    問題数 272025/07/10

    TUMOR MARKERS

    問題数 252025/07/10

    TOXICOLOGY

    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26