Impregnation and Embedding

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Impregnation is also known as

INFILTRATION

The most important step between infiltration and embedding serves as one of the important steps of embedding.

ORIENTATION

What are the different types of microtome

ROTARY, VIBRATING, CRYOSTAT

A frozen cabinet which houses microtome inside

CRYOSTAT

This is the removal of clearing agent and filling the tissue cavities to give firm consistency

INFILTRATION, IMPREGNATION

Impregnation or infiltration promotes firm consistency to tissues giving an easier?

HANDLING, CUTTING

This may happen to the tissue when section due to inadequate impregnation as the tissue is soft

CRUMBLE

This may happen to the tissue when floated out in a water bath due to inadequate impregnation as the tissue is soft.

BREAK UP

This is where we placed the tissue during embedding for orientation

MOLD

What is the main purpose of infiltrating media

FILL UP HOLE

What is the end product of embedding

TISSUE BLOCK

Process by which the impregnated tissue is placed into precisely arranged position in a mold containing a medium which is then allowed to solidify

EMBEDDING

What is the other name of embedding

BLOCKING, CASTING

This is where we place the tissue during impregnation

CASSETTE

This is where we place the tissue when we fill up paraffin wax

WAX DISPENSER

What is the required temperature for curing embedded tissue

-5C

Maximum temperature for melting parawaffin wax

60C

This substances cannot be detected when using paraffin wax. This also the disadvantage of using paraffin wax

LIPID, ENZYME

This is used to cover the oriented tissue

CASSETTE

Embedding is done for what step of the tissue processing

SECTION CUTTING

This is formed when the tissue block is run through the section cutting

TISSUE RIBBON

The medium used to infiltrate the tissue is usually the same medium utilized for impregnation and for general purposes is known as

EMBEDDING MEDIUM, RESIN

The most common embedding medium

PARAFFIN WAX

The medium use for embedding hard tissues

CELLOIDIN

This medium is good for embedding tissues for electron microscopy

PLASTIC

Enumerate the required characteristics of infiltrating and embedding medium

SOLUBLE IN PROCESSING FLUID, SUITABLE, MOLTEN BETWEEN 30 TO 60, TRANSPARENT, HOMOGENEOUS, INEXPENSIVE, FLATTEN AFTER RIBBONING, NON-TOXIC

Factors that affects infiltration

LABORATORY TEMPERATURE, NUMBER OF CHANGES, CLEARING AGENT USED, VOLUME

How many temperatures you add to the manufacturers guideline of the paraffin wax when using paraffin oven

2-5C

Minimum number of changes in paraffin wax

Do larger and denser tissues requires more changes true or false

true

The following clearing agents are more difficult to remove

CEDAR WOOD OIL, CHLOROFORM

The following clearing agent are more easy to remove

XYLENE, BENZENE

What is the normal volume of embedding medium as compared to the tissue

25X THE EMBEDDING MEDIUM

This gives paraffin wax harder matrix

POLYMER

How many changes and minutes each changes done in impregnation using paraffin wax in manual processing

4, 15

What is the requirement and how many changes is done in impregnation automatic processing

AGITATION, 2-3

The most common organ that is received in laboratory for histopathology

WOMB

How many minutes that is take in embedding when paraffin wax is used

The measurement of negative pressure of tissue tek in mmHg

400-500

This is used to accelerate the processing urgent biopsies dense and fibrosis connective tissues and delicate

VACUMM

These are the disadvantages of overheating to the tissues

BRITTLENESS, SHRINKAGE, HARDENING

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