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1
Classifications of enzymes are based on_____of the enzyme
CATALYTIC ACTIVITY
2
The international union of biochemistry enzyme commission categorize enzyme to how many classes
6
3
What is the first class of enzyme
OXIDOREDUCTASE, OXIDASE, DEHYDROGENASE
4
Class of enzyme which catalyze oxidation reduction reactions via transfer of electron from one mole to another
OXIDOREDUCTASE
5
Class of enzyme assayed for investigation of cardiac and liver disorders
OXIDOREDUCTASE
6
Examples of oxidoreductase
GLUCOSE OXIDASE, CYTOCHROME OXIDASE, LACTATE DEHYDROGENASE
7
Donor of electron is also known as
OXIDANT
8
Recipient of electron is also known as
REDUCTANT
9
What is the second class of enzyme
TRANSFERASE
10
Class of enzyme move an intact group of atoms (NH2 or PO4) from one molecule to another
TRANSFERASE
11
Class of enzyme that gives information about liver damage
TRANSFERASE
12
Process in which a phosphate group is added to molecule
PHOSPHORYLATION
13
What are the examples of transferase
AST, ALT, CK
14
Class of enzyme which catalyzes the hydrolysis of various chemical bonds
HYDROLASE
15
Class of enzyme in which reactions utilizes water and it is used to break a bond within a molecule leading to the formation of two smaller molecules
HYDROLASE
16
What are the subclasses of hydrolase
ESTERASE, PEPTIDASE, GLYCOSIDASE
17
Subclass of hydrolase in which hydrolyzed or catalyzed ester bonds or lipid molecules
ESTERASE
18
Sunglass of hydrolase which hydrolyzes peptide bonds in proteins and peptides
PEPTIDASE, PROTEASE
19
Subclass of hydrolase which hydrolyzes glycosidic bonds in carbohydrates
GLYCOSIDASE
20
Examples of glycosidase
AMYLASE, AMYLO-1,6-GLUCOSIDASE
21
Examples of peptidase
LEUCINE AMINOPEPTIDASE, PEPSIN
22
Examples of esterase
ACID PHOSPHATASE, ALKALINE PHOSPHATASE, TRIACYLGLYCEROL LIPASE
23
Class of enzyme which catalyzes the breaking of various chemical bonds without the use of water and without oxidation and reduction
LYASE
24
Lyase often result in the formation of a _______ or ____ structure in the molecule
DOUBLE BOND, NEW RING
25
Class of enzyme that are acid in the diagnosis of cellular muscle disorder
LYASE
26
Splits fructose-1, 6-biphosphate into two smaller sugar
ALDOLASE
27
Class of enzyme that catalyze the conversion of molecule into one of its isomers which is a molecule with the same chemical formula but different structural arrangement
ISOMERASE
28
Which class of enzyme is reversible reaction
ISOMERASE
29
The class of enzyme which involves intramolecular rearrangement not addition or removal of atoms and its substrate and product have identical molecular formulas but differ in structure or spatial arrangement
ISOMERASE
30
The type of isomer reaction which geometric isomers of alkenes or cyclic compounds
CIS-TRANS ISOMERISM
31
The type of isomer reaction which amino acids and sugars changing chirality
L-D ISOMERISM
32
An example of alldose-ketose conversion which function is to convert G6P to become F6P
GLUCOSE PHOSPHATE ISOMERASE
33
What is the third class of enzyme
HYDROLASE
34
What is the fourth class of enzyme
LYASE
35
What is the fifth class of enzyme
ISOMERASE
36
What is the six class of enzyme
LIGASE
37
Class of enzyme which catalyze the joining or delegation of two molecules typically using energy derived from atp hydrolysis to form a larger more complex
LIGASE
38
Ligase enzyme reactions are typically_____building up molecules
ANABOLIC
39
Ligase enzyme reactions requires energy input usually in the form
ATP
40
Ligase enzyme which joins dna strands during replication and repair
DNA LIGASE
41
Ligase enzyme which attaches amino acids to the corresponding trna
AMINOACYL-TRNA SYNTHETASE
42
Ligase enzyme which adds carbon dioxide to pyruvate in gluconeogenesis
PYRUVATE CARBOXYLASE
43
What is the first class of enzyme
OXIDOREDUCTASE, OXIDASE, DEHYDROGENASE
44
Converts glutamate to GABA which removes carbon dioxide
GLUTAMATE DECARBOXYLASE
45
Converts pyruvate to acetaldehyde + CO2
PYRUVATE DECARBOXYLASE
46
Refers to the different forms of the same enzyme that exist in an organism
ENZYME VARIANT
47
Structurally distinct forms of an enzyme that catalyzes the same reaction
ISOENZYME, ISOZYME
48
Isoenzyme are usually____multimeric proteins composed of different combinatios of subunits
MULTICHAINED
49
Isoenzymes are often differ in
AMINO ACID COMPOSITION, KINETIC PROPERTIES, PHYSICAL PROPERTIES
50
Km is also known as
MICHAELIS CONSTANT
51
Kinetic properties of isoenzymes are measured in
KM, VMAX
52
Physical properties of isoenzymes are measured in
ELECTROPHORETIC MOBILITY, PH STABILITY
53
Enzyme used to differentiate liver Vs bone disease
ALKALINE PHOSPHATASE
54
Enzyme used to assess pancreatic disorder
AMYLASE
55
Characteristics of isoenzyme which differ in net charge and size affecting how they migrate in an electric field
ELECTROPHORETIC MOBILITY
56
Characteristics of isoenzyme which binds differently due to variation in surface charge, allowing for fractionation and identification
ION EXCHANGE RESIN MOBILITY
57
Enzymes of similar catalytic activity but are specie specific
HETEROENZYME
58
Importance of heteroenzyme
COMPARATIVE ENZYMOLOGY, IMMUNOLOGIC STUDY, THERAPEUTIC DEVELOPMENT
59
Used to differentiate RBC ACP and Prostatic ACP
2% FORMALIN
60
Which ACP inhibit 2% formalin
RBC ACP
61
Genetically transmitted enzyme important in defining biochemical characteristics of an individual
ALLOENZYME
62
Stable and active generally secreted by the liver directly to the plasma
PLASMA SPECIFIC ENZYME
63
Examples of plasma specific enzyme
COAGULATION ENZYME, COMPLEMENT ENZYME, LIPOPROTEIN LIPASE
64
Enzymes mostly originated from cells or tissues, and their presence in plasma is typically due to cell injury, secretion, or turnover
NON PLASMA SPECIFIC ENZYME
65
Secreted into plasma at high rate but also rapidly cleared
ENZYME OF SECRETION
66
Examples of enzyme of secretion
AMYLASE, LIPASE
67
Perform metabolic functions inside the cells where they are synthesized and usually released into plasma only when cells are damaged orr undergo necrosis
ENZYME ASSOCIATED WITH CELLULAR METABOLISM
68
Examples of enzyme associated with cellular metabolism
ALT, AST
69
Enzymes that are found in only one specific cellular location and involved in cytosolic metabolism such as alkaline phosphatase
UNILOCULAR ENZYME
70
Enzymes that are distributed in 2 distinct cellular compartments
BILOCULAR ENZYME
71
Location of unilocular enzyme
CELL SAP
72
Location of bilocular enzyme
CELL SAP, MITOCHONDRIA
73
Enzyme kinetics which is unchanges acts as catalyst
ENZYME
74
Enzyme kinetics which is temporary intermediate
ENZYME SUBSTRATE COMPLEX
75
Enzyme kinetics which is molecule acted upon
SUBSTRATE
76
Enzyme kinetics which is transformed substrate
PRODUCT
77
Enzyme responsible for clot formation
SERINE PROTEASE
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