Sectioning Microtomy Part 2

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Enumerate the different kinds of microtome knives

UNDER COMPOUND MICROSCOPE, UNDER ELECTRON MICROSCOPE, DISPOSABLE BLADE, SAFETY RAZOR BLADE

Different knives focuses under the compound microscope

PLANE CONCAVE KNIFE, PLANE WEDGE KNIFE, BICONCAVE

Focuses under the electron microscope

DIAMOND EDGE KNIFE, GLASS KNIFE

Knife with about 25 mm length

PLANE CONCAVE KNIFE

Knife with about 100 mm length

PLANE WEDGE KNIFE

Knife with about 120 mm length

BICONCAVE KNIFE

Profile of knife which one side is flat while the other is concave

PLANE CONCAVE KNIFE

What kind of microtome and what block is used in less concave sides

SLIDING, CELLOIDIN EMBEDDED TISSUE

What microtome and what tissue block is used in more concave sides

BASE SLEDGE, ROTARY, PARAFFIN EMBEDDED

Profile of knife with both sides are straight

PLANE WEDGE

Plane wedge knife are used for what section

FROZEN, CELLOIDIN EMBEDDED

What microtome uses plane wedge knife

BASE SLEDGE, SLIDING

What section uses biconcave knife

PARAFFIN EMBEDDED

What microtome uses biconcave knife

ROTARY

Profile of knife which used for resin blocks for ultra thin microscopy and for electron microscopy

DIAMOND EDGE

It is generally used for trimming semi thin section

GLASS

How many micrometers thin in disposable plates

2-4

This is quoted in disposable blades to enhance strength and prolong cutting life

PLATINUM 6, CHROMIUM 7

This reduces cutting resistance and minimal friction in disposable blades. This is also in crystat

TEFLON

Very thin cutting edge manufacture from high quality stainless steel

DISPOSABLE BLADES

How many micrometers do safety razor blades have

Safety razor blades are used for

PARTIALLY CALCIFIED TISSUE, PARAFFIN, FROZEN

What is the clearance correct angle for cutting specimen

0° clearance angle causes what to the specimen

PRESSURE

Too step knife angle causes what to the specimen

DEFORMATION

Correct angle reduces friction which preventing what to the section

COMPRESSION

Angle between two facets that form the cutting edge which about 27 to 32°

BEVEL ANGLE

Larger the bevel angle, the sharper the knife true or false

FALSE

Too small bevel angle permits what of the edge

ELASTIC DISTORTION

The width of the two faucet which makes the cutting edge of knife has a recommended measurement of how many millimeters

0.1-0.6

The angle between lower edge/bevel of the knife and tissue block

CLEARANCE

How many degrees should be maintained in clearance angle to maintain even sections

5-10

The angle between the upper edge/bevel of the knife and the tissue block

RAKE

This is also called as the angle of cutting

WEDGE

Give less compression to the tissue block and produces a smooth plastic flow type during sectioning

HIGH RAKE, LOW CLEARANCE

Recommended angle for soft tissues but it need to be reduced for harder tissues

HIGH RAKE

This must be of good quality steel

CUTTING EDGE

It is a criterion where cutting edge is able to reflect light and split hair strand across ot it their own resistance

VON MOL'S CRITERION

Microtome knives are assess in a paraffin wax which it should cut how many um thick that is examined under what magnification

2-4, 100X

What are the 2 types of microtome knife sharpening

HONING, STROPPING

This is the hard sharpening which removes nicks, blemishes, and irregularities

HONING

This is the polishing, and sharpening of cutting edge, which remove burrs during honing that it can be performed before sectioning a delicate specimen

STROPPING

COMPH Lesson 5

COMPH Lesson 5

73問 • 2年前

Jef Marc Valencia · 42問 · 2年前

Discussion 1

Discussion 1

42問 • 2年前

Jef Marc Valencia · 42問 · 2年前

Dis 1

Dis 1

42問 • 2年前

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Shift -Systematic Error

Shift -Systematic Error

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LEVEY – JENNINGS CHART

LEVEY – JENNINGS CHART

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WESTGARD MULTIRULE CHART

WESTGARD MULTIRULE CHART

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Random vs Systematic

Random vs Systematic

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Researcj Design

Researcj Design

48問 • 2年前

Jef Marc Valencia · 40問 · 2年前

Dis 1 mid

Dis 1 mid

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COMPM LESSON 6-7

COMPM LESSON 6-7

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Discussion -2 Six Sigma

Discussion -2 Six Sigma

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Sigma Levels

Sigma Levels

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COMPH- LESSON 8

COMPH- LESSON 8

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Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

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WAVELENGTH Color Table

WAVELENGTH Color Table

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Quiz Announcement ANALYTICAL METHODS & INSTRUMENTATION

Quiz Announcement ANALYTICAL METHODS & INSTRUMENTATION

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QUIz ANNOUNCEMENT part 2

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Electrochem

Electrochem

62問 • 1年前

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ELEC TROCH

ELEC TROCH

18問 • 1年前

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TRANSCRIPTION

TRANSCRIPTION

49問 • 1年前

LIS

LIS

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Carbohydrates

Carbohydrates

16問 • 1年前

DIFFERENT PATHWAYS IN GLUCOSE METABOLISM

DIFFERENT PATHWAYS IN GLUCOSE METABOLISM

III. GLUCOSE MEASUREMENT

III. GLUCOSE MEASUREMENT

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Copper Reductions

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7問 • 1年前

Exp.8

Exp.8

QUIZ 8/9

QUIZ 8/9

Immunology

Immunology

Jef Marc Valencia · 22問 · 1年前

Immunology part 2

Immunology part 2

22問 • 1年前

Immunology 3

Immunology 3

Lesson 4

Lesson 4

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PROTEINS

PROTEINS

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PROTEIN 2

PROTEIN 2

34問 • 1年前

PROTEIN 3

PROTEIN 3

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Serum Albumin Determination

Serum Albumin Determination

56問 • 1年前

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CC lec debris

CC lec debris

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NPN

NPN

44問 • 1年前

NPN 2

NPN 2

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Materials management

Materials management

33問 • 1年前

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Liver

Liver

57問 • 1年前

LESSON 10

LESSON 10

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hyperlipoproteinemia

hyperlipoproteinemia

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OBLIGATE INTRACELLULAR BACTERIA

OBLIGATE INTRACELLULAR BACTERIA

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THE SPIROCHETES

THE SPIROCHETES

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PYOGENIC GRAM POSTIVE

PYOGENIC GRAM POSTIVE

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Jef Marc Valencia · 31問 · 1年前

Introduction

Introduction

31問 • 1年前

Introduction Page 2

Introduction Page 2

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Introduction pg 3

Introduction pg 3

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Introduction table

Introduction table

32問 • 1年前

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Introduction-body

Introduction-body

14問 • 1年前

NK-Immunity

NK-Immunity

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meet 2

meet 2

45問 • 1年前

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discussion 3

discussion 3

38問 • 1年前

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dis 1

dis 1

25問 • 1年前

Immuno vs anti

Immuno vs anti

Jef Marc Valencia · 47問 · 1年前

Immune 3

Immune 3

47問 • 1年前

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dis 4

dis 4

46問 • 1年前

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TRANSUDATIVE Vs. EXUDATIVE

TRANSUDATIVE Vs. EXUDATIVE

12問 • 1年前

Jef Marc Valencia · 75問 · 1年前

dis 5

dis 5

75問 • 1年前

Jef Marc Valencia · 9問 · 1年前

Preserved Tissue Examination Steps by definition

Preserved Tissue Examination Steps by definition

9問 • 1年前

FIXATIVES

FIXATIVES

Jef Marc Valencia · 70問 · 1年前

Long discussion 1

Long discussion 1

70問 • 1年前

Jef Marc Valencia · 10問 · 1年前

ALDEHYDE-FIXATIVES

ALDEHYDE-FIXATIVES

10問 • 1年前

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lactate dehydrogenase

lactate dehydrogenase

24問 • 1年前

FIXATIVES-METALLIC

FIXATIVES-METALLIC

Jef Marc Valencia · 5問 · 1年前

FIXATIVES-CHROMATE

FIXATIVES-CHROMATE

5問 • 1年前

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Specialized Macrophages

Specialized Macrophages

10問 • 1年前

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LYMPHOPOIESIS

LYMPHOPOIESIS

14問 • 1年前

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PICRIC ACID-FIXATIVE

PICRIC ACID-FIXATIVE

5問 • 1年前

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immune 4

immune 4

33問 • 1年前

FIXATIVES-ALCOHOL

FIXATIVES-ALCOHOL

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Immunoglobulins

Immunoglobulins

40問 • 1年前

ARTIFACTS

ARTIFACTS

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FIXATIVES QUIZ COPY

FIXATIVES QUIZ COPY

11問 • 1年前

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MAJOR HISTOCOMPATIBILITY

MAJOR HISTOCOMPATIBILITY

26問 • 1年前

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Introduction

Introduction

58問 • 1年前

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OTHERS-FIXATIVES

OTHERS-FIXATIVES

5問 • 1年前

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MEGAKARYOPOLESIS

MEGAKARYOPOLESIS

34問 • 1年前

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DECALCIFICATION

DECALCIFICATION

51問 • 1年前

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DEHYDRATION

DEHYDRATION

64問 • 1年前

Blood Specimen Collection

Blood Specimen Collection

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CLEARING

CLEARING

7問 • 1年前

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CEARING AGENT

CEARING AGENT

19問 • 1年前

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INTRODUCTION

INTRODUCTION

27問 • 1年前

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Introduction

Introduction

51問 • 1年前

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Methods of Determination

Methods of Determination

12問 • 1年前

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ASPARTATE AMINOTRANSFERASE

ASPARTATE AMINOTRANSFERASE

11問 • 1年前

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ALANINE AMINOTRANSFERASE

ALANINE AMINOTRANSFERASE

15問 • 1年前

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Creatinine Kinase

Creatinine Kinase

34問 • 1年前

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LACTATE DEHYDROGENASE

LACTATE DEHYDROGENASE

27問 • 1年前

Jef Marc Valencia · 41問 · 1年前

ACID PHOSPHATASE

ACID PHOSPHATASE

41問 • 1年前