ENZYME IMMUNOASSAY

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

These are naturally occurring molecules react with suitable substrates to produce product

ENZYME

Enzymes serves as _in a certain biological reaction

CATALYST

Enzyme are measured via_method

COLORIMETRIC

Enzymes produce this following reactions

CHROMOGENIC, FLUOROGENIC, LUMINESCENT

This immunoassay test is cheap readily available and has long shelf life

ENZYME IMMUNOASSAY

Typical enzymes used in enzyme immunoasay

HORSERADISH PEROXIDASE, GLUCOSE 6 PHOSPHATE DEHYDROGENASE, ALKALINE PHOSPHATASE, BETA D GALACTOSIDASE

The most common enzyme used enzyme immunoase as this has the highest turnover and detection rate

HORSERADISH PEROXIDASE

This requires steps to physically separate (washing) free from bound analyte

HETEROGENOUS IMMUNOASSAY

This has no separation step is necessary (washing) as enzyme activity diminishes when binding of an antibody and antigen occurs

HOMOGENOUS IMMUNOASSAY

What are the heterogeneous in enzyme immunoassay

COMPETITIVE

c-ELISA Transcribe: Unknown: Principle: Result:

COMPETITIVE ENZYME LINK IMMUNOSORBENT ASSAY, PATIENT ANTIGEN, COMPETITIVE BINDING ASSAY, INVERSELY PROPORTIONAL

This type of enzyme immunoassay is used for measuring small antigens that are relatively pure such as insulin and estrogen

COMPETITIVE ENZYME LINK IMMUNOSORBENT ASSAY

It does not participate in the initial AGAB binding reaction. It measures antibody production to infectious agents that are difficult to isolate.

INDIRECT ENZYME LINK IMMUNOSORBENT ASSAY

Indirect enzyme link immunosorbent assays are preferred screening test for detecting antibody to

HIV, HEP-A, HEP-C

Preferred subject for testing as it is low cost and does not require the course of infection

ANTIBODY

nc-ELISA First reagent: Unknown: Second reagent: Principle: Result:

SOLID PHASE ANTIGEN, PATIENT ANTIBODY, ENZYME LABELED ANTI IMMUNOGLOBULIN, NON-COMPETITIVE BINDING ASSAY, DIRECTLY PROPORTIONAL

Immunoenzymometric Test unknown

ANTIGEN

Capture assays is also known as

IMMUNOLOGICAL SANDWICH

Capture assays First reagent: Unknown: Second reagent: Principle: Result:

ANTIBODY, MULTIVALENT ANTIGEN, ENZYME LABELED ANTIBODY, NON-COMPETITIVE BINDING ASSAY, DIRECTLY PROPORTIONAL

Type of enzyme immunoassay which only reacts if the patient antigen is bivalent

IMMUNOLOGICAL SANDWICH

This is used to stop the enzymatic reaction as enzymes are catalyst which means reactions continuous

SUBSTRATE

It is based on the principle of change in enzyme activity as a specific agab combination occurs

HOMOGENOUS ENZYME IMMUNOASSAY

Reagent antigen in homogeneous immunoase is labeled with an

ENZYME TAG

It is the emission of light caused by a chemical reaction typically an oxidation reaction producing an excited molecule that decays back to its original ground state

CHEMILUMINESCENCE

What are the common substances in chemiluminescence

LUMINOL, ACRIDINIUM ESTER, RUTHENIUM DERIVATIVE, NITROPHENYL OXALATE

Complement fixation test is discovered by

BORDET, GENGOU

Complement are used for

LYSIS

Complement precautions

UNSTABLE, HEAT LABILE

How to inactivate a complement in serum Heat: DURATION:

56C, 30 MIN

Chemicals that inactivate complement

ACID, ALKALI, ALCOHOL

Complement are stable in Room Temp for: Ref temp for: -20C for: -40C for:

24 HOUR, 3-4 DAY, 1 MON, 6 MON

Compliment are in activated by

VIGOROUS AGITATION, RESISTANT ORGANISM, HEMOLYSIS, LOSS OF ACTIVITY

These are susceptible to complement

ERYTHROCYTE, LEUKOCYTE, THROMBOCYTE, GRAM NEGATIVE BACTERIA

These are resistant to complement

GRAM POSITIVE BACTERIA, MOST MAMMALIAN CELL, PLANT CELL, YEAST, MOLD

It is when agab reaction occurs as this is an inactivated or fix such that it will be unavailable to cause cell license after the addition of indicator sheep rbc

LYTIC COMPLEMENT FIXATION TEST

Lytic complement fixation test detects

COMPLEMENT FIXING ANTIBODY

What is the positive result of lytic complement fixation test

NON-LYSIS OF INDICATOR CELL

What is the indicator cell of lytic complement fixation test

SHEEP RED BLOOD CELL

This is present in shift red blood cell causing a lysis in the presence of complement

HEMOLYSIN

What is the unknown in the lytic complement fixation test

ANTIBODY

What is the preferred source of complement

GUINEA PIG

Identify the reaction principle

IMMUNOLOGICAL SANDWICH

Identify the reaction principle

NON-COMPETITIVE ENZYME LINKED IMMUNOSORBENT ASSAY

COMPH Lesson 5

COMPH Lesson 5

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Discussion 1

Discussion 1

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Dis 1

Dis 1

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Shift -Systematic Error

Shift -Systematic Error

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LEVEY – JENNINGS CHART

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WESTGARD MULTIRULE CHART

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Random vs Systematic

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Researcj Design

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Dis 1 mid

Dis 1 mid

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COMPM LESSON 6-7

COMPM LESSON 6-7

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Discussion -2 Six Sigma

Discussion -2 Six Sigma

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Sigma Levels

Sigma Levels

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COMPH- LESSON 8

COMPH- LESSON 8

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Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

Long Discussion ANALYTICAL METHODS & INSTRUMENTATION

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WAVELENGTH Color Table

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Quiz Announcement ANALYTICAL METHODS & INSTRUMENTATION

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Electrochem

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ELEC TROCH

ELEC TROCH

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TRANSCRIPTION

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LIS

LIS

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Carbohydrates

Carbohydrates

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DIFFERENT PATHWAYS IN GLUCOSE METABOLISM

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III. GLUCOSE MEASUREMENT

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Copper Reductions

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Exp.8

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QUIZ 8/9

QUIZ 8/9

Immunology

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Immunology part 2

Immunology part 2

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Immunology 3

Immunology 3

Lesson 4

Lesson 4

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PROTEINS

PROTEINS

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PROTEIN 2

PROTEIN 2

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PROTEIN 3

PROTEIN 3

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Serum Albumin Determination

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CC lec debris

CC lec debris

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NPN

NPN

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NPN 2

NPN 2

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Materials management

Materials management

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Liver

Liver

57問 • 1年前

LESSON 10

LESSON 10

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hyperlipoproteinemia

hyperlipoproteinemia

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OBLIGATE INTRACELLULAR BACTERIA

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24問 • 1年前

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THE SPIROCHETES

THE SPIROCHETES

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PYOGENIC GRAM POSTIVE

PYOGENIC GRAM POSTIVE

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Introduction

Introduction

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Introduction Page 2

Introduction Page 2

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Introduction pg 3

Introduction pg 3

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Introduction table

Introduction table

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Introduction-body

Introduction-body

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NK-Immunity

NK-Immunity

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meet 2

meet 2

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discussion 3

discussion 3

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dis 1

dis 1

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Immuno vs anti

Immuno vs anti

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Immune 3

Immune 3

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dis 4

dis 4

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TRANSUDATIVE Vs. EXUDATIVE

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dis 5

dis 5

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Preserved Tissue Examination Steps by definition

Preserved Tissue Examination Steps by definition

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FIXATIVES

FIXATIVES

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Long discussion 1

Long discussion 1

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ALDEHYDE-FIXATIVES

ALDEHYDE-FIXATIVES

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lactate dehydrogenase

lactate dehydrogenase

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FIXATIVES-METALLIC

FIXATIVES-METALLIC

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FIXATIVES-CHROMATE

FIXATIVES-CHROMATE

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Specialized Macrophages

Specialized Macrophages

10問 • 1年前

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LYMPHOPOIESIS

LYMPHOPOIESIS

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PICRIC ACID-FIXATIVE

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immune 4

immune 4

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FIXATIVES-ALCOHOL

FIXATIVES-ALCOHOL

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Immunoglobulins

Immunoglobulins

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ARTIFACTS

ARTIFACTS

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FIXATIVES QUIZ COPY

FIXATIVES QUIZ COPY

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MAJOR HISTOCOMPATIBILITY

MAJOR HISTOCOMPATIBILITY

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Introduction

Introduction

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OTHERS-FIXATIVES

OTHERS-FIXATIVES

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MEGAKARYOPOLESIS

MEGAKARYOPOLESIS

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DECALCIFICATION

DECALCIFICATION

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DEHYDRATION

DEHYDRATION

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Blood Specimen Collection

Blood Specimen Collection

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CLEARING

CLEARING

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CEARING AGENT

CEARING AGENT

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INTRODUCTION

INTRODUCTION

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Introduction

Introduction

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Methods of Determination

Methods of Determination

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ASPARTATE AMINOTRANSFERASE

ASPARTATE AMINOTRANSFERASE

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ALANINE AMINOTRANSFERASE

ALANINE AMINOTRANSFERASE

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Creatinine Kinase

Creatinine Kinase

34問 • 1年前

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LACTATE DEHYDROGENASE

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Jef Marc Valencia · 41問 · 1年前

ACID PHOSPHATASE

ACID PHOSPHATASE

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