SPERM 2

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Enumerate testing done in Microsocopic analysis of sperm

MOTILITY, COUNT, CONCENTRATION, MORPHOLOGY, VIABILITY

Main factor which affects the penetrating ability of the sperm cells through the cervical mucus barrier

SPERM MOTILITY

Sperm motility are examined under

20 HPF

Instrument used to transfer sperm to the slide in sperm motility

POSITIVE DISPLACEMENT PIPETTE

How many sperm are counted in alternative procedure

200

Normal sperm motility

>50% CATEGORY ABC, >25% CATEGORY AB

Provides objective testing sperm motility based on the sperm velocity and trajectory (direction of motion) and also determines sperm concentration and morphology

COMPUTER ASSISTED SEMEN ANALYSIS

Normal value for sperm count

>40 MILLION/EJACULATE

Normal sperm concentration

>20 MILLION/ML

Counting chambers that can be used in sperm count

MAKLER, NEUBAUER HEMOCYTOMETER

Counting chambers that utilizes undiluted specimen which uses heating to immobilized sperm

MAKLER

It uses 4 corner square and large center square to count sperm, both sides should be loaded and 10% agree to each other

NEUBAUER HEMOCYTOMETER

Microscope used in the sperm analysis

PHASE CONTRAST, BRIGHT FIELD

Rule in counting sperm using neubauer

INVERTED L

Enumerate diluting fluids that can be used in sperm analysis

1.5% SODIUM BICARBONATE, 2.1% FORMALIN, 0.5% CHLORAZENE, 4.1% FORMALIN IN SODIUM BICARBONATE, 5% SODIUM BICARBONATE+1% PHENOL IN DISTILLED H20, CHILLED DISTILLED H2O

Termed used to define WBCS seen in sperm analysis

ROUND CELL

Term used to define immature sperm cells seen in sperm analysis

SPERMATID

Indicates inflammation or infection of the reproductive system leading to infertility

>1 MILLION/ML

Indicates disruption of spermatogenesis

>1 MILLION/ML

Sperm count is reported as

PER EJACULATE

Decrease in the number of sperm cells or presence of few motile cells

OLIGOSPERMIA

Seen in unilateral and bilateral hypotrophic testes and hypothyroidism

OLIGOSPERMIA

Refers to the complete or total absence of spermatozoa

AZOSPERMIA

Seen in bilateral underdeveloped testes, obstruction, and infection w/ gonorrhea

AZOSPERMIA

Presence of dead or immobile sperm cell

NECROSPERMIA

Seen in hypertrophic testes and decrease fructose level

NECROSPERMIA

Contains mitochondria that provides energy for flagellar motion

NECK, MIDDLE PIECE

Approx length of sperm tail

45 UM

Tail of sperm should exhibit what motion

WHIP LIKE

Normal shape of head of the sperm

OVAL

Measurement of the head of sperm

5 UM, 3 UM

Acrosomal cap of head the sperm should occupy

1/2 OF HEAD, 2/3 NUCLEUS

Sperm morphology should be examined under

OIL IMMERSION FIELD

What is being reported in sperm morphology

PERCENTAGE OF ABNORMAL SPERM

Stain of choice for sperm morphology

PAPANICOLAOU

Stains used in sperm morphology

PAPANICOLAOU, GIEMSA, WRIGHTS

Normal value of routine sperm morphology

>30%

Normal value for Krugers Strict criteria sperm morphology

>14%

Differentiation and enumeration of round cells

CALCULATION OF ROUND CELL

Formula in Calculation of Round cells

C=(NXS)/100

In Calculation of Round cells, What variable stands for the number of Round cells

In Calculation of Round cells, What variable stands for the number of spermatids and/ or leukocytes counted

In Calculation of Round cells, What variable stands for the sperm concentration in millions per ml

How many sperm are counted in viability testing

100

Test if patient has normal sperm concentration w/ markedly decreased motility

SPERM VIABILITY TEST, BLOOMS TEST, EOSIN NEGROSIN

In Eosin Negrosin test, living cells appears as

BLUISH WHITE

In Eosin Negrosin test, Dead cells appears as

RED AGAINST PURPLE BACKGROUND

Normal value for sperm viability test

75% LIVING CELL/100 SPERM CELL

Low levels of Fructose are associated w/

ANDROGEN DEFICIENCY, LOW TESTOSTERONE LEVEL, SEMINAL VESICLE INSUFFICIENCY

Screening test for Fructose test

RESORCINOL TEST

Confirmatory test for fructose level which used to quantitate fructose level in semen

SPECTROPHOTOMETRIC

How many ml of the reagent used in resorcinol test

9 ML

After boiling mixed specimen in resorcinol test, what color is imparted

ORANGE RED

Normal value of Fructose level of sperm

>13 UMOL/EJACULATE

It destroys blood and testes barrier which can be detected to both genders

ANTISPERM ANTIBODY

Screening procedure to detect IgG Ab in ASA

MIXED AGGLUTINATION REACTION

Semen sample is incubated w/ AHG and a suspension of latex particles or treated RBCs coated w/ IgG

MIXED AGGLUTINATION REACTION

Positive result for mixed agglutination reaction

>50% AGGLUTINATION

More specific test which detects IgG, IgM, and IgA antibodies and demonstrate what area of the sperm the autoantibodies are affecting

IMMUNOBEAD TEST

Positive result for Immunobead test

>20% SPERM W/ BOUND IMMUNOBEAD

Post coital test to determine the quality of the cervical mucus and the ability of sperm cells to penetrate it.

SIMS HUHNER TEST

Determines the presence of choline used also in crime investigation

FLORENCE TEST

Reagents used in Florence Test

POTASSIUM IODIDE, IODINE CRYSTAL

Positive result of Florence test

BROWN RHOMBIC, NEEDLE CRYSTAL

Determines the presence of spermine

BARBIEROS TEST

Reagent used in Barbieros Test

PICRIC ACID, TRICHLOROACETIC ACID

Positive result of Barbieros test

YELLOW LEAF STRUCTURE

Determines the tenacity of the mucus

SPINBARKEIT TEST

Specimens are test at monthly intervals, beginning at 2 months after vasectomy and continuing until two consecutive monthly specimens shows no spermatozoa

POST VASECTOMY SEMEN ANALYSIS

COMPH Lesson 5

COMPH Lesson 5

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LIS

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Lesson 4

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PROTEINS

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PROTEIN 3

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CC lec debris

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NPN

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NPN 2

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Materials management

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LESSON 10

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Introduction

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Introduction Page 2

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Introduction pg 3

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Introduction table

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meet 2

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discussion 3

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dis 1

dis 1

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Immune 3

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dis 4

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dis 5

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Long discussion 1

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FIXATIVES-CHROMATE

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LYMPHOPOIESIS

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immune 4

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Introduction

Introduction

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OTHERS-FIXATIVES

OTHERS-FIXATIVES

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DECALCIFICATION

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INTRODUCTION

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Introduction

Introduction

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Methods of Determination

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