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SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

  • Jef Marc Valencia

    • 問題数 74 • 9/6/2025

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  • 1

    General considerations in specimen collection for mycology

    AVOID CONTAMINANT, STAGE OF INFECTION, ADEQUATE QUANTITY

  • 2

    Specimen should be collected___and processed

    ASEPTICALLY, STAT

  • 3

    Stage of infection when fungal elements are most abundant

    ACTIVE STAGE

  • 4

    This ensures higher diagnostic yield

    ADEQUATE QUANTITY

  • 5

    Site of collection which scrapes form the active and advancing edge of the lesion after cleaning w/ 70% alcohol

    SKIN

  • 6

    Site of collection from clippings or scrapings from beneath the nail plate

    NAIL

  • 7

    Region where nail collection is obtained

    DISTAL, SUBUNGUAL

  • 8

    Hair plucks from the base of the infected follicle is obtained using

    STERILE FORCEP, WOODS LUMP

  • 9

    Exudates collected via drainage or pus from the infected site via

    ASPIRATION, SWABBING

  • 10

    Preferable collection of exudates

    ASPIRATION

  • 11

    Site of collection which used for the identifiation of fluorescent eg. dermatophytes

    HAIR

  • 12

    What are the site of collection for systemic infections

    SPUTUM, URINE, BLOOD, CSF

  • 13

    Site of collection for systemic infection collected in early morning via deep cough specimen like M. tuberculosis requiring repeated collection

    SPUTUM

  • 14

    Site of collection for systemic infection collected in early morning as it is more concentrated requiring stat centrifugation used for Histoplasma and Yeast Cell identification

    URINE

  • 15

    Site of collection for systemic infection collected in buffy coat for suspected disseminates

    BLOOD

  • 16

    Site of collection for systemic infection used for suspected fungal meningitis

    CSF

  • 17

    Widely used and general purpose fungal medium containing cycloheximide and acidic pH inhibiting bacterial growth

    SABOURAUDS DEXTROSE AGAR

  • 18

    pH that inhibits bacterial growth in SDA medium

    5.6

  • 19

    This inhibits saprophytic fungi but allows pathogenic fungi to grow in SDA agar

    CYCLOHEXIMIDE

  • 20

    Antibiotics that can be supplemented in SDA agar

    CHLORAMPHENICOL, GENTAMICIN, PENICILLIN, STREPTOMYCIN

  • 21

    Enriched medium containing many nutrients used for fastidious and dimorphic fungi which antibiotics could also be used

    BRAIN HEART INFUSION AGAR

  • 22

    Examples of fungi which requires BHIA agar

    HISTOPLASMA CAPSULATUM, BLASTOMYCES DERMATITIDIS, COCCIDIOIDES IMMITIS

  • 23

    Medium used in routine mycology laboratory that provides nutrients for both c'mon colds and fastidious fungi

    SABOURAUDS BRAIN HEART INFUSION AGAR

  • 24

    SABHI agar is a combination of what agar

    BRAIN HEART INFUSION, SABOURAUDS DEXTROSE AGAR

  • 25

    Agar used for c'mon fungi in SABHI

    SABOURAUDS DEXTROSE AGAR

  • 26

    Agar used for fastidious fungi in SABHI

    BRAIN HEART INFUSION AGAR

  • 27

    Used for ID and to enhance fungal specific structures

    SUBCULTURING MEDIA

  • 28

    Promotes pigment and conidial production providing good contrast background for colony morphology

    POTATO DEXTROSE AGAR

  • 29

    Potato Dextrose Agar is good for what mycoses

    ASPERGILLUS, PENICILLIUM

  • 30

    Incubation temperature for mold phase or filamentous mycoses

    25-30C

  • 31

    Incubation temperature for yeast phase mycoses

    37C

  • 32

    This fungi must be incubate at both temperatures to be demonstrated

    DIMORPHIC FUNGI

  • 33

    Duration of incubation of fungi

    2-4 WEEK

  • 34

    Dermatophytes fungi may take longer to incubate for how many weeks

    6 WEEK

  • 35

    Macroscopic characteristics for the Identification of fungi

    COLOR/PIGMENT, TEXTURE, GROWTH RATE, TOPOGRAPHY, CONDITION OF GROWTH

  • 36

    This appears as small, fluffy, lacking of diagnostic structures only in vegetative hyphae with limited growth w/o spores

    YOUNG COLONY

  • 37

    Well developed w/ conidiospores describe based on texture

    MATURE COLONY

  • 38

    Rapid growers which are mostly saprobes, mucor, rhizopus may take for how many days

    <5 DAY

  • 39

    Intermediate growers which are dermatophytes may take for how many days

    6-10 DAY

  • 40

    Slow growers which are systemic fungi like histoplasma and blastomycosis may take for how many days

    >11 DAY, 4-6 WEEK

  • 41

    Macroscopic features which examined on both obverse and reverse of the colony

    PIGMENTATION

  • 42

    Pigmentation which shows front surface colour and texture

    OBVERSE

  • 43

    Pigmentation which shows diffusable metabolite

    REVERSE

  • 44

    This fungi often display distinct obverse and reverse pigmentation

    DERMATOPHYTE

  • 45

    This fungi display dark pigmentation

    DEMATIACEOUS FUNGI

  • 46

    What colour do Microsporum canis pigmentation displays

    YELLOW ORANGE

  • 47

    What colour do Trichophyton rubrum pigmentation displays

    DEEP RED-REDDISH

  • 48

    What colour do dematiaceous fungi pigmentation displays

    OLIVE GREEN, BROWN, BLACK

  • 49

    Non pigmented fungi which is a counterpart of dematiaceous fungi

    HYALINE

  • 50

    Fungal texture

    COTTONY

  • 51

    Fungal texture

    VELVETY

  • 52

    Fungal texture

    GRANULAR

  • 53

    Fungal texture

    GLABROUS

  • 54

    Macroscopic appearance of fungal colony type and amount of aerial hyphae fungal production

    TEXTURE

  • 55

    Leathery/waxy and smooth with minimal mycelium

    GLABROUS

  • 56

    Example of fungi which displays glabrous texture

    DERMATOPHYTE, TRICHOPHYTON

  • 57

    Short aerial hyphae with soft surface

    VELVETY, SUEDE LIKE

  • 58

    What fungi exhibits a velvety texture

    ASPERGILLUS, PENICILLIUM

  • 59

    Resembles bacterial colonies, smooth with no aerial mycelium

    YEAST LIKE

  • 60

    What fungi exhibits yeast like texture

    CANDIDA ALBICAN

  • 61

    Long aerial hyphae with fluffy surface that could lift the lid

    COTTONY, WOOLLY, FLOCCOSE

  • 62

    Heavy sporulation, rough, powdery, dry and abundant seen in condia spores

    GRANULAR, POWDERY

  • 63

    What fungi exhibits granular and powdery texture

    MICROSPORUM, TRICHOPHYTON

  • 64

    What macroscopic characteristics observed in SDA and PDA plates

    TOPOGRAPHY

  • 65

    Smooth and even surface topography

    FLAT

  • 66

    Radial grooves like a bicycle wheel topography

    RUGOSE

  • 67

    Ridges arranged in folds with wrinkles, uneven surface seen in older mature colony topography

    FOLDED

  • 68

    Central depression volcano like topography

    CRATERIFORM

  • 69

    Rough wart like projections topography

    VERRUCOSE

  • 70

    Convoluted folds resembling brain tissue topography

    CEREBRIFORM

  • 71

    This macroscopic appearance shows early clue

    GROWTH RATE

  • 72

    Fungi that are rapid growers

    MUCOR

  • 73

    Fungi that slow growers

    HISTOPLASMA

  • 74

    This features are combined for accurate fungal identification

    MACROSCOPIC, MICROSCOPIC

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    問題数 132025/05/06

    MCQ-CSF2

    問題数 242025/05/03

    TREMATODES

    問題数 72025/05/06

    INTRODUCTION

    問題数 362025/06/10

    INTRODUCTION TABLE

    問題数 412025/06/10

    CLASSFICATION OF ENZYME

    問題数 472025/06/11

    ENZYME VARIANTS

    問題数 412025/06/11

    FACTORS AFFECTING ENZYME ACTIVITY

    問題数 902025/06/12

    INTRODUCTION TO ENZYMOLOGY-MCQ

    問題数 412025/06/26

    CLASS AND VARIANT OF ENZYME-MCQ

    問題数 772025/06/26

    PITUITARY GLAND PART 2

    問題数 52025/07/04

    THYROID GLAND

    問題数 712025/07/04

    PARATHYROID GLAND

    問題数 122025/07/04

    ADRENAL GLAND

    問題数 772025/07/04

    SEX GLANDS

    問題数 352025/07/04

    OTHER GLANDS

    問題数 82025/07/04

    ACID BASE BALANCE

    問題数 512025/07/10

    TRACE ELEMENT

    問題数 792025/07/10

    VITAMINS

    問題数 272025/07/10

    TUMOR MARKERS

    問題数 252025/07/10

    TOXICOLOGY

    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26