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Hemocytometry

  • Jef Marc Valencia

    • 問題数 55 • 9/24/2024

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  • 1

    3 methods in hemocytometry

    TURBIDIMETRIC, MICROSCOPIC, AUTOMATED

  • 2

    Method in which it is simple as observation wherein the more cell there, the more it appears to its characteristics

    TURBIDIMETRIC

  • 3

    A manual method, wherein sample is prepared by dilution and counting them under microscope.

    MICROSCOPIC

  • 4

    It is the most routine cell-counting method in hematology laboratory

    AUTOMATED

  • 5

    Method when counts exceed the linearity of an instrument or when an instrument is non-functional and there is no backup instrument, or in an extreme situation when testing is done in the field.

    MANUAL

  • 6

    It is the numerical evaluation of formed elements and estimation of the number of blood cells in a known volume of blood.

    HEMOCYTOMETRY

  • 7

    Estimated numbers of RBC's in uL

    SEVERAL MILLIONS

  • 8

    Estimated numbers of WBC's in uL

    SEVERAL THOUSANDS

  • 9

    Estimated numbers of platelets in uL

    SEVERAL HUNDRED THOUSANDS

  • 10

    Too much overlapping of cells my occur and it is impossible to count them if we simply focus the blood sample into a microscope. Therefore, what method should we do to DISPERSE them?

    DILUTION

  • 11

    These are used in preparation of dilution

    DILUTING PIPET

  • 12

    What are the 2 types of diluting pipets

    AUTOMATIC, NON AUTOMATIC

  • 13

    An automatic pipet that can be filled via capillary action. It is similar to RBC pipet however, it is attached with a scuking tube to allow entry of blood.

    TRENNER

  • 14

    An automatic pipet that has a red bulb with single graduation on top of the bulb which is 101.

    TRENNER

  • 15

    An automatic plastic disposable pipet that already contains the diluent inside the reservoir of the container.

    UNOPETTE

  • 16

    Unopette is only available to RBC counting.

    FALSE

  • 17

    Diluent of RBC in unopette

    NSS

  • 18

    Diluent of WBC in unopette

    ACETIC ACID

  • 19

    Diluent of platelets in unopette

    AMMONIUM OXALATE

  • 20

    A non-automatic pipet that is commonly used in manual procedure.

    THOMA

  • 21

    What is the usual dilution for WBC pipet

    1:20

  • 22

    What is the usual dilution in RBC pipet

    1:200

  • 23

    What is the usual blood volume

    0.5

  • 24

    This is the highest and lowest dilution that a pipet can contain

    DILUTION RANGE

  • 25

    The least volume of blood that we can add which has the highest dilution

    0.1

  • 26

    The maximum amount of blood that we can add which has the lowest dilution

    1

  • 27

    Recommended dilution for anticipated WBC count of 0.1-3.0

    1:10

  • 28

    Recommended dilution for anticipated WBC count of 3.1-30

    1:20

  • 29

    Recommended dilution for anticipated WBC count of >30

    1:100

  • 30

    Recommended dilution for anticipated WBC count of 100

    1:200

  • 31

    This characteristics of diluting fluid as it evaporates and it becomes concentrated causing red cells to crenate.

    STABLE

  • 32

    These characteristics of diluting fluid should not distort the blood cells preventing swelling.

    PRESERVATIVE ACTION

  • 33

    These characteristics of diluting fluid let cells settle faster and prevent them from moving for easier counting.

    HIGH SPECIFIC GRAVITY

  • 34

    Too high specific gravity can cause

    CRENATION

  • 35

    These characteristics of diluting fluid capable of maintaining the shape of the cells

    BUFFER ACTION

  • 36

    Reagent that should be used in WBCs

    HYPOTONIC FLUID

  • 37

    RBCs can obscure WBC count causing difficulty in counting. Therefore, hypotonic solution is needed that will not cause any changes to WBCs but it cause RBCs to

    HEMOLYZE

  • 38

    Reagent used for RBCs

    ISOTONIC FLUID

  • 39

    The most fragile cells which needed to maintains morphology to be able to count them

    RBC

  • 40

    This serves as a guide in counting cells

    HEMOCYTOMETER

  • 41

    Most commonly used hemocytomter

    IMPROVED NEUBAUER

  • 42

    The space between the cover slip and ruled chamber

    DEPTH

  • 43

    Most common types of hemocytometer according to rulings

    FUCHS ROSENTHAL, SPEIRS LEVY, IMPROVED NEUBAUER

  • 44

    Hemocytometer ruling used in counting fewer number of cells like eosinophil and Basophil

    SPEIRS LEVY

  • 45

    Most ideal diluting fluid of RBC containing Formalin and 1% of Sodium Citrate. This serves in preserving cell

    FORMOL CITRATE, DACIES

  • 46

    This will make the nucleus of the WBCs more prominent as it contains Glacial Acetic acid and 1% aqueous Gentian Violet.

    TURKS SOLUTION

  • 47

    The most ideal diluting fluid of WBC

    1-3% ACETIC ACID

  • 48

    How many drops of blood to be discarded after dilution

    2-3

  • 49

    How long for diluted sample to settle

    2-10 MIN

  • 50

    What objective to be used in counting WBCs

    LPO

  • 51

    What objective to be used in counting RBCs

    HPO

  • 52

    What objective to be used in counting platelets

    HPO

  • 53

    When counting the hemocytometer, it always starts on what part?

    TOP MOST LEFT, FIRST SQUARE

  • 54

    This is the rule where all cell touching the L-shaped will be counted wherein RIGHT and LEFT most will not be counted

    INVERTED L RULE

  • 55

    Which of these hemocytometers have a DEPTH of 0.2 mm

    FUCHS ROSENTHAL, SPEIRS LEVY

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    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26