QUANTIFICATION OF HBF

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

Alkali denaturation methods for Fetal Hemoglobin

BETKE, SINGER

Quantitation of the percentage of HbF in the blood

ALKALI DENATURATION

This serves as positive control for HbF alkali denaturation

FETAL UMBILICAL BLOOD

HbF reference value using modification by betke

0.2-1.0%

Recommended HbF quantitation range of HbF by NCCLS

2-40%

This must be performed when HbF is less than 2%

RADIO IMMUNOMETRIC ASSAY

This must be performed when HbF is more than 40%

COLUMN CHROMATOGRAPHY

Also known as__Determines the presence of fetal red cells in the maternal circulation to assess whether the distribution of HbF in all red cell is the same. It is useful for the diagnosis of Hemolytic Disease of the Newborn

ACID ELUTION, MODIFICATION OF KLEIHAUER AND BETKE BY SHEPARD

In Acid Elution, Hemoglobins other than Hb F are eluted from the red cells on an air-dried blood film by a

CITRIC ACID PHOSPHATE BUFFER

What is the pH of citric acid phosphate buffer

3.3

This hemoglobin only remains in the fixed cell in acid elution

FETAL HEMOGLOBIN

Stain used for HbF acid elution

EHRLICH ACID HEMATOXYLIN

Counter Stain used for HbF acid elution

ERYTHROSIN

The appearance of RBCs w/ HbF after acid elution

BRIGHT PINK-RED

The appearance of RBCs w/o HbF after acid elution

GHOST CELL

This condition shows even distribution of HbF among red cells

HEREDITARY PERSISTENCE OF FETAL HEMOGLOBIN

This condition heterogenous distribution of HbF among red cell

THALASSEMIA, HEMOGLOBINOPATHY

For the detection and preliminary identification of both normal and abnormal Hbs.

CELLULOSE ACETATE ELECTROPHORESIS

pH used in Cellulose Acetate Electrophoresis

8.4-8.6

Detects small amounts of either Hb A or F in the presence or large amounts of the others. This reveals small amounts of adult Hbs A and S present at birth in cord blood. Separates hb that migrate together on cellulose acetate.

CITRATE AGAR ELECTROPHORESIS

pH used in Citrate Agar Electrophoresis

6.0-6.2

Citrate Agar Electrophoresis is based on the principle of which interactions among Hb variants, agar and citrate buffer ions; altered electrical charge of the various Hbs at acid pH

SEPARATION OF HEMOGLOBIN

Refers to a disease state involving the hemoglobin molecule result form a genetic mutation in one or more genes that hemoglobin synthesis

HEMOGLOBINOPATHY

Thalassemic disorders

QUANTITATIVE DEFECT

Decreased or non-existent production of one or more globin chain

THALASSEMIC DISORDER

Type of Thalassemic disorder c'mon in asians

ALPHA

Type of Thalassemic disorder c'mon in Mediteranean

BETA

Type of Thalassemic disorder c'mon in Africa

ALPHA, BETA

Result from the alteration of the DNA genetic code for the chains

STRUCTURAL HEMOGLOBINOPATHY

Structural hemoglobinopathy

QUALITATIVE DEFECT

Also known as __Amino acid substitution when O2 is reduced at the tissue level polymerizes leading to the formation of tactoid crystals which cause the cells to become rigid.

HEMOGLOBIN S, SICKLE CELL ANEMIA

Hb S gene has provided resistance from what parasite

PLASMODIUM FALCIPARUM

Characteristics of HbF

HIGH OXYGEN AFFINITY, DECREASED 2,3 DPG AFFINITY, ACID AND ALKALI RESISTANT, SLOWER THAN HBA1

This serves as negative control for HbF alkali denaturation

HBA1, ADULT BLOOD

Reagent used for HbF alkalie denaturation

DRABKINS

An alkali solution that denatures HbA1 but not HbF in alkali denaturation

SODIUM HYDROXIDE

This reagent stops reaction in HbF alkali denaturation

AMMONIUM SULFATE

Filtrate of HbF in alkali denaturation is measured via

SPECTROPHOTOMETER

Conditions that may elevate HbF level

HEMOGLOBINOPATHY, B-THALASSEMIA, HEREDITARY PERSISTENCE OF FETAL HEMOGLOBIN

A condition caused by mutation of the synthesis od gamma chain leading to increase HbF

HEREDITARY PERSISTENCE OF FETAL HEMOGLOBIN

C'monly used quantitation method of HbF in blood banking

ACID ELUTION

Antigen present in Rh+ blood

D ANTIGEN

Immunoglobulins capable to cross at placenta

IMMUNOGLOBULIN G

In alkaline pH, hemoglobin proteins migrate to what polar of the electrophoresis

ANODE

Hemoglobin proteins migration to anode fastest to slowest in Celullose Acetate Electrophoresis

A, F, SDG, A2CEO

What hemoglobin migrates to cathode in citrate agar electrophoresis

A, F

What hemoglobin migrates to anode in citrate agar electrophoresis

S, C

Hemoglobin synthess is normal but molecules is altered amino acid sequence in the globin chains

QUALITATIVE HEMOGLOBINOPATHY

Hemoglobin synthesis is reduced but molecules do not affect amino acid sequence of the amino acid sequence of the globin chains.

QUANTITATIVE HEMOGLOBINOPATHY

Mutation

QUALITATIVE

Deletion

QUANTITATIVE

Most c'mon hemoglobinopathy

HEMOGLOBIN S

Hemoglobinopathies are c'monly caused by mutation in what part of beta globin amino acid

6TH, GLUTAMINE

Hb S is result of glutamine mutation to

VALINE

Hb S molecule polymerizes leading to the formation of __causing cell to become rigid

TACTOID CRYSTAL

Inheritance of Hemoglobinopathy which is severe as HbA1 is no longer produced

HOMOZYGOUS

Inheritance of hemoglobinopathy which is less severe as about 50% of HbA1 is still produced

HETEROZYGOUS

Measures the degree of anisocytosis

RED CELL DISTRIBUTION WIDTH

In sickle cell, anemia is usually severe causing RDW to

INCREASE

Sickle cell anemia progression may lead to what conditions

RETARDED GROWTH, SEXUAL MATURATION

RBC inclusions due to DNA remnants

HOWELL JOLLY BODY

RBC inclusions due to iron deposits

PAPPENHEIMER BODY

Howell jolly body may be associated with

HYPOSPLENISM, ASPLENISM, SEVERE HEMOLYTIC ANEMIA

This refers to any situation that produces excessive deoxygenation of RBCS crises

SICKLE CELL CRISES

Refers to sickle crises when Sickle cell obstruct blood vessels leading to cyanosis and necrosis

VASOOCCLUSIVE CRISES

HbA compensates for HbS which patients usually have no symptoms unless in cases of extreme tissue hypoxia

SICKLE CELL TRAIT, HB AS

Percentage of HbS in sickle cell trait

30-45%

Screening test for sickling hemoglobin which turbidity is read against a newsprint/reader card with thin black lines

DITHIONITE SOLUBILITY TUBE TEST

Lysing agent in diothionite solubility tube test

SAPONIN

Deoxidizing agent which binds to oxygen in dithionite solubility tube test

SODIUM DITHIONITE

Anemia with numerous target cells that polymerizes under low O2 tension which structures differs from HbS

Hb CC, HOMOZYGOUS Hb CC

Hb CC is result of glutamine mutation to

LYSINE

Hb CC crystals appearance

HEXAGONAL, ROD SHAPED

RBCs are slightly hypochromic target cells

HB C TRAIT

Milder than Hb SS that looks like pocketbook cell

Hb SC

Hb SC crystals appearance

HAND IN GLOVE, WASHINGTON MONUMENT

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