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QUANTIFICATION OF HBF

  • Jef Marc Valencia

    • 問題数 76 • 10/12/2025

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  • 1

    Alkali denaturation methods for Fetal Hemoglobin

    BETKE, SINGER

  • 2

    Quantitation of the percentage of HbF in the blood

    ALKALI DENATURATION

  • 3

    This serves as positive control for HbF alkali denaturation

    FETAL UMBILICAL BLOOD

  • 4

    HbF reference value using modification by betke

    0.2-1.0%

  • 5

    Recommended HbF quantitation range of HbF by NCCLS

    2-40%

  • 6

    This must be performed when HbF is less than 2%

    RADIO IMMUNOMETRIC ASSAY

  • 7

    This must be performed when HbF is more than 40%

    COLUMN CHROMATOGRAPHY

  • 8

    Also known as__Determines the presence of fetal red cells in the maternal circulation to assess whether the distribution of HbF in all red cell is the same. It is useful for the diagnosis of Hemolytic Disease of the Newborn

    ACID ELUTION, MODIFICATION OF KLEIHAUER AND BETKE BY SHEPARD

  • 9

    In Acid Elution, Hemoglobins other than Hb F are eluted from the red cells on an air-dried blood film by a

    CITRIC ACID PHOSPHATE BUFFER

  • 10

    What is the pH of citric acid phosphate buffer

    3.3

  • 11

    This hemoglobin only remains in the fixed cell in acid elution

    FETAL HEMOGLOBIN

  • 12

    Stain used for HbF acid elution

    EHRLICH ACID HEMATOXYLIN

  • 13

    Counter Stain used for HbF acid elution

    ERYTHROSIN

  • 14

    The appearance of RBCs w/ HbF after acid elution

    BRIGHT PINK-RED

  • 15

    The appearance of RBCs w/o HbF after acid elution

    GHOST CELL

  • 16

    This condition shows even distribution of HbF among red cells

    HEREDITARY PERSISTENCE OF FETAL HEMOGLOBIN

  • 17

    This condition heterogenous distribution of HbF among red cell

    THALASSEMIA, HEMOGLOBINOPATHY

  • 18

    For the detection and preliminary identification of both normal and abnormal Hbs.

    CELLULOSE ACETATE ELECTROPHORESIS

  • 19

    pH used in Cellulose Acetate Electrophoresis

    8.4-8.6

  • 20

    Detects small amounts of either Hb A or F in the presence or large amounts of the others. This reveals small amounts of adult Hbs A and S present at birth in cord blood. Separates hb that migrate together on cellulose acetate.

    CITRATE AGAR ELECTROPHORESIS

  • 21

    pH used in Citrate Agar Electrophoresis

    6.0-6.2

  • 22

    Citrate Agar Electrophoresis is based on the principle of which interactions among Hb variants, agar and citrate buffer ions; altered electrical charge of the various Hbs at acid pH

    SEPARATION OF HEMOGLOBIN

  • 23

    Refers to a disease state involving the hemoglobin molecule result form a genetic mutation in one or more genes that hemoglobin synthesis

    HEMOGLOBINOPATHY

  • 24

    Thalassemic disorders

    QUANTITATIVE DEFECT

  • 25

    Decreased or non-existent production of one or more globin chain

    THALASSEMIC DISORDER

  • 26

    Type of Thalassemic disorder c'mon in asians

    ALPHA

  • 27

    Type of Thalassemic disorder c'mon in Mediteranean

    BETA

  • 28

    Type of Thalassemic disorder c'mon in Africa

    ALPHA, BETA

  • 29

    Result from the alteration of the DNA genetic code for the chains

    STRUCTURAL HEMOGLOBINOPATHY

  • 30

    Structural hemoglobinopathy

    QUALITATIVE DEFECT

  • 31

    Also known as __Amino acid substitution when O2 is reduced at the tissue level polymerizes leading to the formation of tactoid crystals which cause the cells to become rigid.

    HEMOGLOBIN S, SICKLE CELL ANEMIA

  • 32

    Hb S gene has provided resistance from what parasite

    PLASMODIUM FALCIPARUM

  • 33

    Characteristics of HbF

    HIGH OXYGEN AFFINITY, DECREASED 2,3 DPG AFFINITY, ACID AND ALKALI RESISTANT, SLOWER THAN HBA1

  • 34

    This serves as negative control for HbF alkali denaturation

    HBA1, ADULT BLOOD

  • 35

    Reagent used for HbF alkalie denaturation

    DRABKINS

  • 36

    An alkali solution that denatures HbA1 but not HbF in alkali denaturation

    SODIUM HYDROXIDE

  • 37

    This reagent stops reaction in HbF alkali denaturation

    AMMONIUM SULFATE

  • 38

    Filtrate of HbF in alkali denaturation is measured via

    SPECTROPHOTOMETER

  • 39

    Conditions that may elevate HbF level

    HEMOGLOBINOPATHY, B-THALASSEMIA, HEREDITARY PERSISTENCE OF FETAL HEMOGLOBIN

  • 40

    A condition caused by mutation of the synthesis od gamma chain leading to increase HbF

    HEREDITARY PERSISTENCE OF FETAL HEMOGLOBIN

  • 41

    C'monly used quantitation method of HbF in blood banking

    ACID ELUTION

  • 42

    Antigen present in Rh+ blood

    D ANTIGEN

  • 43

    Immunoglobulins capable to cross at placenta

    IMMUNOGLOBULIN G

  • 44

    In alkaline pH, hemoglobin proteins migrate to what polar of the electrophoresis

    ANODE

  • 45

    Hemoglobin proteins migration to anode fastest to slowest in Celullose Acetate Electrophoresis

    A, F, SDG, A2CEO

  • 46

    What hemoglobin migrates to cathode in citrate agar electrophoresis

    A, F

  • 47

    What hemoglobin migrates to anode in citrate agar electrophoresis

    S, C

  • 48

    Hemoglobin synthess is normal but molecules is altered amino acid sequence in the globin chains

    QUALITATIVE HEMOGLOBINOPATHY

  • 49

    Hemoglobin synthesis is reduced but molecules do not affect amino acid sequence of the amino acid sequence of the globin chains.

    QUANTITATIVE HEMOGLOBINOPATHY

  • 50

    Mutation

    QUALITATIVE

  • 51

    Deletion

    QUANTITATIVE

  • 52

    Most c'mon hemoglobinopathy

    HEMOGLOBIN S

  • 53

    Hemoglobinopathies are c'monly caused by mutation in what part of beta globin amino acid

    6TH, GLUTAMINE

  • 54

    Hb S is result of glutamine mutation to

    VALINE

  • 55

    Hb S molecule polymerizes leading to the formation of __causing cell to become rigid

    TACTOID CRYSTAL

  • 56

    Inheritance of Hemoglobinopathy which is severe as HbA1 is no longer produced

    HOMOZYGOUS

  • 57

    Inheritance of hemoglobinopathy which is less severe as about 50% of HbA1 is still produced

    HETEROZYGOUS

  • 58

    Measures the degree of anisocytosis

    RED CELL DISTRIBUTION WIDTH

  • 59

    In sickle cell, anemia is usually severe causing RDW to

    INCREASE

  • 60

    Sickle cell anemia progression may lead to what conditions

    RETARDED GROWTH, SEXUAL MATURATION

  • 61

    RBC inclusions due to DNA remnants

    HOWELL JOLLY BODY

  • 62

    RBC inclusions due to iron deposits

    PAPPENHEIMER BODY

  • 63

    Howell jolly body may be associated with

    HYPOSPLENISM, ASPLENISM, SEVERE HEMOLYTIC ANEMIA

  • 64

    This refers to any situation that produces excessive deoxygenation of RBCS crises

    SICKLE CELL CRISES

  • 65

    Refers to sickle crises when Sickle cell obstruct blood vessels leading to cyanosis and necrosis

    VASOOCCLUSIVE CRISES

  • 66

    HbA compensates for HbS which patients usually have no symptoms unless in cases of extreme tissue hypoxia

    SICKLE CELL TRAIT, HB AS

  • 67

    Percentage of HbS in sickle cell trait

    30-45%

  • 68

    Screening test for sickling hemoglobin which turbidity is read against a newsprint/reader card with thin black lines

    DITHIONITE SOLUBILITY TUBE TEST

  • 69

    Lysing agent in diothionite solubility tube test

    SAPONIN

  • 70

    Deoxidizing agent which binds to oxygen in dithionite solubility tube test

    SODIUM DITHIONITE

  • 71

    Anemia with numerous target cells that polymerizes under low O2 tension which structures differs from HbS

    Hb CC, HOMOZYGOUS Hb CC

  • 72

    Hb CC is result of glutamine mutation to

    LYSINE

  • 73

    Hb CC crystals appearance

    HEXAGONAL, ROD SHAPED

  • 74

    RBCs are slightly hypochromic target cells

    HB C TRAIT

  • 75

    Milder than Hb SS that looks like pocketbook cell

    Hb SC

  • 76

    Hb SC crystals appearance

    HAND IN GLOVE, WASHINGTON MONUMENT

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    問題数 222025/04/29

    MCQ-CHEMICAL EXAMINATION OF URINE PART 2

    問題数 792025/05/03

    PERICARDIAL FLUID

    問題数 662025/05/04

    MCQ-SPERM

    問題数 592025/05/03

    MCQ-SPERM 2

    問題数 882025/05/03

    MCQ-AMNIOTIC FLUID

    問題数 732025/05/08

    HEMOFLAGELLATES

    問題数 682025/05/04

    Sex disorder

    問題数 602025/05/05

    NEMATODES

    問題数 62025/05/06

    MCQ-PERICARDIAL FLUID

    問題数 592025/05/11

    PHASMID NEMATODES

    問題数 52025/05/06

    HOOKWORMS

    問題数 92025/05/06

    MCQ-CSF

    問題数 942025/05/03

    BLOOD AND TISSUE NEMATODE

    問題数 82025/05/06

    PLATYHELMINTHS

    問題数 132025/05/06

    MCQ-CSF2

    問題数 242025/05/03

    TREMATODES

    問題数 72025/05/06

    INTRODUCTION

    問題数 362025/06/10

    INTRODUCTION TABLE

    問題数 412025/06/10

    CLASSFICATION OF ENZYME

    問題数 472025/06/11

    ENZYME VARIANTS

    問題数 412025/06/11

    FACTORS AFFECTING ENZYME ACTIVITY

    問題数 902025/06/12

    INTRODUCTION TO ENZYMOLOGY-MCQ

    問題数 412025/06/26

    CLASS AND VARIANT OF ENZYME-MCQ

    問題数 772025/06/26

    PITUITARY GLAND PART 2

    問題数 52025/07/04

    THYROID GLAND

    問題数 712025/07/04

    PARATHYROID GLAND

    問題数 122025/07/04

    ADRENAL GLAND

    問題数 772025/07/04

    SEX GLANDS

    問題数 352025/07/04

    OTHER GLANDS

    問題数 82025/07/04

    ACID BASE BALANCE

    問題数 512025/07/10

    TRACE ELEMENT

    問題数 792025/07/10

    VITAMINS

    問題数 272025/07/10

    TUMOR MARKERS

    問題数 252025/07/10

    TOXICOLOGY

    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 542025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26