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MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

  • Jef Marc Valencia

    • 問題数 54 • 9/6/2025

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  • 1

    This provides immediate ID but a negative finding does not rule out mycotic infection because wrong area of collection may have been sampled and only few condia may be present

    MICROSCOPIC STUDY OF FUNGI

  • 2

    Wet preparations are simple cover slipped preparations examined under what objective

    LOW POWER

  • 3

    C'mon specimen sources for wet preparations

    SKIN, SCRAPING, NAIL, HAIR, BIOPSY

  • 4

    Rapid and inexpensive stain for direct examination that clears tissues debris by digesting keratin

    POTASSIUM HYDROXIDE

  • 5

    This component of KOH prevents crystallization

    GLYCEROL

  • 6

    Concentration of KOH

    10-15%

  • 7

    This component of KOH may be added for contrast

    METHYLENE BLUE

  • 8

    Ratio of methylene blue for KOH

    2:1

  • 9

    How many minutes do KOH are allow to stand

    10-15 MIN, HEAT GENTLY

  • 10

    This speed up digestion eliminating the need of heating and also added to enhance penetration

    DIMETHYL SULFOXIDE

  • 11

    Wha specimen is not recommended to used Dimethyl sulfoxide

    HAIR, THIN SKIN SCALE

  • 12

    Fluorescent stain that binds chitin and cellulose viewed under UV light

    CALCOFLUOR WHITE

  • 13

    Calcofluor white may added with what component for rapid detection

    POTASSIUM HYDROXIDE

  • 14

    Calcofluor white uses what background

    DARK BACKGROUND

  • 15

    What colour do calcofluor white imparts

    FLUORESCENT APPLE-GREEN, BLUE-WHITE

  • 16

    Used for encapsulated yeasts that appears as a refractile clear halo around yeast cells

    INDIA INK, NIGROSIN, NEGATIVE STAIN

  • 17

    What fungi is specifically used India Ink

    CRYPTOCOCCUS NEOFORMAN

  • 18

    India Ink may be diluted with

    WATER

  • 19

    Used for detection of intracellular fungi

    WRIGHTS, GIEMSA

  • 20

    Wrights and Giemsa are useful for what fungi

    HISTOPLASMA CAPSULATUM

  • 21

    What colour of the fungal elements imparts in wrights, giemsa, and periodic acid schiff stain

    RED/PURPLE

  • 22

    What colour of the background requires in Wrights and Giemsa stain

    GREEN

  • 23

    Special stain for tissue section that is not often performed

    HEMATOXYLIN AND EOSIN

  • 24

    What colour do fungi appear in hematoxylin and eosin

    PINKISH-BLUE

  • 25

    Sensitive stain that uses green background that stains fungal cell wall great for localizing yeast cells hyphae

    PERIODIC ACID SCHIFF

  • 26

    The gold standard in histopath for fungi staining

    METHENAMINE SILVER, GOMORI

  • 27

    What colour do fungi appear in gomoris stain

    BLACK

  • 28

    What colour of the background do gomoris stain uses

    LAVENDER GRAY

  • 29

    Specific to capsules that highlights mucopolysaccharide and cryptococcus neoformans

    MEYERS MUCICARMINE

  • 30

    What colour do fungi imparts in meyers mucicarmine

    ROSE-RED

  • 31

    Highly specific stain sensitive detection but too expensive

    FLUORESCENT ANTIBODY STAINING

  • 32

    Toxic but the standard/permanent w/ detailed slide mounting fluid in mycology

    LACTOPHENOL COTTON BLUE

  • 33

    Component of LCPB that Acts as fixative

    LACTIC ACID

  • 34

    Component of LCPB that acts as fungicidal agent

    PHENOL

  • 35

    Component of LCPB that stains chitin in fungal cell wall

    COTTON BLUE

  • 36

    An Alternative mounting fluid that preserves good structure reducing shrinking and distortion

    POLYVINYL ALCOHOL

  • 37

    Rapid method by teasing fungal elements with needles or slides but mmay distort morphology

    TEASE MOUNT

  • 38

    It is pressed onto fungal colony that placed on slide with LCPB that preserves colony morphology

    CELLULOSE TAPE PREPARATION

  • 39

    What are the disadvantages of Cellulose tape preparation

    NOT STERILE, NOT FOR LONG TERM PRESERVATION, AEROSOL SPREAD OF CONIDIA

  • 40

    Inoculated with fungi and covers lipped allowing observation of delicate linkages between conidia and conidiophores preserving natural arrangement of spores that is used for fungi with fragile structures

    MICROSLIDE CULTURE, AGAR BLOCK METHOD

  • 41

    Are of the agar block in microslide method

    1 CM

  • 42

    Rapid screening test for Candida albicans

    GERM TUBE TEST

  • 43

    Initial filamentous outgrowth yeast cell longer than the parent cell about half as wide w/ parallel sides and asexual reproductive structure

    GERM TUBE

  • 44

    Candida albicans is positive when germ tube forms for how many hours after incubation in serum

    2-3 HOUR

  • 45

    This is to maintain viable cultures for research or teaching and achieved by slowing fungal metabolism

    PRESERVATION OF FUNGAL CULTURE

  • 46

    Preservation of fungal cultures can be achieved by decreasing

    OXYGEN, NUTRIENT AVAILABILITY

  • 47

    Method of preservation for fungal cultures

    FORMALDEHYDE METHOD, PARA FILM SEALING, MINERAL OIL OVERLAY, FREEZING, STERILE WATER METHOD

  • 48

    Method of fungi preservation which requires 10 drops of the reagent absorbed by cotton and placed in tube or jar with cultures

    FORMALDEHYDE METHOD

  • 49

    Method of preservation which seals cultures tubes and plates reducing evaporation

    PARAFILM SEALING

  • 50

    Storage of parafilm sealing

    6-12 MOS/BOTH TEMP

  • 51

    Method of preservation that covers slant with sterile mineral oil mimitng oxygen which is anerobic but messy to handle

    MINERAL OIL OVERLAY

  • 52

    Method of preservation used in research studies that is viable for 1-2 years

    FREEZING

  • 53

    Freezing method of preservation are stored in what temperature

    -20C/-70C

  • 54

    Method of preservation which cultures is suspended in 2 ML sterile distilled water which is simple, inexpensive, but effective

    STERILE WATER METHOD

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    問題数 722025/07/13

    DRUG OF ABUSE

    問題数 952025/07/13

    TOXINS

    問題数 572025/07/13

    TDM

    問題数 942025/07/13

    ENDOCRINOLOGY

    問題数 962025/07/16

    ENDROCRINOLOGY PART 2

    問題数 462025/07/16

    FACTORS AFFECTING ENZYME ACTIVITY-MCQ

    問題数 892025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ

    問題数 992025/07/17

    ENZYME OF CLINICAL SIGNIFICANCE-MCQ2

    問題数 872025/07/18

    MEDICAL TERMINOLOGIES

    問題数 362025/08/17

    INTRODUCTION TO HEMA 1

    問題数 292025/08/17

    LABORATORY SAFETY

    問題数 232025/08/18

    TERMINOLOGIES

    問題数 512025/08/21

    LABORATORY 1

    問題数 182025/08/22

    LABORATORY 3

    問題数 532025/08/22

    HEMATOPOIESIS

    問題数 622025/08/23

    THEORIES ON BLOOD FORMATION

    問題数 552025/08/30

    ERYTHROPOIESIS

    問題数 462025/08/30

    LEUKOPOIESIS

    問題数 1002025/08/30

    LEUKOPOIESIS II AND MONOPOIESIS

    問題数 422025/09/01

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/24

    MYCOLOGY PROPER

    問題数 712025/09/01

    FUNGAL PATHOGENESIS

    問題数 352025/09/01

    CLASSIFICATION OF FUNGI

    問題数 452025/09/06

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS

    問題数 742025/09/06

    Mind mapping

    問題数 92025/09/07

    INTRODUCTION TO MYCOLOGY MCQ

    問題数 322025/09/13

    MYCOLOGY PROPER MCQ

    問題数 592025/09/13

    FUNGAL PATHOGENESIS MCQ

    問題数 372025/09/13

    CLASSIFICATION OF FUNGI MCQ

    問題数 332025/09/13

    SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

    問題数 742025/09/13

    MCQ-MICROSCOPIC STUDY, CULTURE EXAMINATION, AND PRESERVATION OF FUNGI

    問題数 532025/09/14

    THE SUPEFICIAL FUNGI

    問題数 342025/09/12

    THE CUTANEOUS FUNGI

    問題数 582025/09/13

    SUBCUTANEOUS FUNGI

    問題数 552025/09/13

    SYSTEMIC FUNGI

    問題数 282025/09/13

    LYMPHOPOIESIS

    問題数 442025/09/07

    MEGAKARYOPOIESIS

    問題数 342025/09/07

    INTRODUCTION TO HEMA1 MCQ

    問題数 452025/09/10

    HEMATOPOIESIS

    問題数 582025/09/14

    THEORIES ON BLOOD FORMATION

    問題数 462025/09/14

    ERYTHROPOIESIS MCQ

    問題数 412025/09/14

    LEUKOPOIESIS MCQ

    問題数 942025/09/10

    LEUKOPOIESIS II AND MONOPOIESIS MCQ

    問題数 392025/09/14

    LYMPHOPOIESIS MCQ

    問題数 392025/09/10

    MEGAKARYOPOIESIS MCQ

    問題数 392025/09/10

    BLOOD COLLECTION

    問題数 352025/09/10

    LABORATORY SAFETY

    問題数 212025/09/17

    LABORATORY 1

    問題数 162025/09/17

    LABORATORY 3

    問題数 472025/09/17

    BLOOD COLLECTION MCQ

    問題数 792025/09/17

    DEMATIACEOUS OPPORTUNISTIC FUNGI

    問題数 522025/09/30

    PENICILLUS

    問題数 212025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA IN CLUSTER

    問題数 162025/10/08

    HYALINE SEPTATE GROUP W/ CONIDIA BORNE SINGLY

    問題数 122025/10/08

    ZYGOMYCETE/HYALINE SEPTATE GROUP

    問題数 492025/10/08

    YEAST AND YEAST LIKE ORGANISMS

    問題数 722025/10/15

    HEMOCYTOMETRY

    問題数 832025/10/03

    HEMOGLOBIN

    問題数 1002025/10/04

    HEMOGLOBIN PART 2

    問題数 262025/10/05

    OXYGEN DISSOCIATION CURVE

    問題数 282025/10/14

    QUANTIFICATION OF HBF

    問題数 762025/10/12

    YEAST AND YEAST LIKE ORGANISMS

    問題数 702025/10/26