SPECIMEN COLLECTION, CULTURE, AND COLONIAL CHARACTERISTICS MCQ

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

General considerations in specimen collection for mycology

AVOID CONTAMINANT, STAGE OF INFECTION, ADEQUATE QUANTITY

Specimen should be collected___and processed

ASEPTICALLY, STAT

Stage of infection when fungal elements are most abundant

ACTIVE STAGE

This ensures higher diagnostic yield

ADEQUATE QUANTITY

Site of collection which scrapes form the active and advancing edge of the lesion after cleaning w/ 70% alcohol

SKIN

Site of collection from clippings or scrapings from beneath the nail plate

NAIL

Region where nail collection is obtained

DISTAL, SUBUNGUAL

Hair plucks from the base of the infected follicle is obtained using

STERILE FORCEP, WOODS LAMP

Exudates collected via drainage or pus from the infected site via

ASPIRATION, SWABBING

Preferable collection of exudates

ASPIRATION

Site of collection which used for the identifiation of fluorescent eg. dermatophytes

HAIR

What are the site of collection for systemic infections

SPUTUM, URINE, BLOOD, CSF

Site of collection for systemic infection collected in early morning via deep cough specimen like M. tuberculosis requiring repeated collection

SPUTUM

Site of collection for systemic infection collected in early morning as it is more concentrated requiring stat centrifugation used for Histoplasma and Yeast Cell identification

URINE

Site of collection for systemic infection collected in buffy coat for suspected disseminates

BLOOD

Site of collection for systemic infection used for suspected fungal meningitis

CSF

Widely used and general purpose fungal medium containing cycloheximide and acidic pH inhibiting bacterial growth

SABOURAUDS DEXTROSE AGAR

pH that inhibits bacterial growth in SDA medium

5.6

This inhibits saprophytic fungi but allows pathogenic fungi to grow in SDA agar

CYCLOHEXIMIDE

Antibiotics that can be supplemented in SDA agar

CHLORAMPHENICOL, GENTAMICIN, PENICILLIN, STREPTOMYCIN

Enriched medium containing many nutrients used for fastidious and dimorphic fungi which antibiotics could also be used

BRAIN HEART INFUSION AGAR

Examples of fungi which requires BHIA agar

HISTOPLASMA CAPSULATUM, BLASTOMYCES DERMATITIDIS, COCCIDIOIDES IMMITIS

Medium used in routine mycology laboratory that provides nutrients for both c'mon colds and fastidious fungi

SABOURAUDS BRAIN HEART INFUSION AGAR

SABHI agar is a combination of what agar

BRAIN HEART INFUSION, SABOURAUDS DEXTROSE AGAR

Agar used for c'mon fungi in SABHI

SABOURAUDS DEXTROSE AGAR

Agar used for fastidious fungi in SABHI

BRAIN HEART INFUSION AGAR

Used for ID and to enhance fungal specific structures

SUBCULTURING MEDIA

Promotes pigment and conidial production providing good contrast background for colony morphology

POTATO DEXTROSE AGAR

Potato Dextrose Agar is good for what mycoses

ASPERGILLUS, PENICILLIUM

Incubation temperature for mold phase or filamentous mycoses

25-30C

Incubation temperature for yeast phase mycoses

37C

This fungi must be incubate at both temperatures to be demonstrated

DIMORPHIC FUNGI

Duration of incubation of fungi

2-4 WEEK, 6 WEEK

Macroscopic characteristics for the Identification of fungi

COLOR/PIGMENT, TEXTURE, CONDITION OF GROWTH, GROWTH RATE, TOPOGRAPHY

This appears as small, fluffy, lacking of diagnostic structures only in vegetative hyphae with limited growth w/o spores

YOUNG COLONY

Well developed w/ conidiospores describe based on texture

MATURE COLONY

Rapid growers which are mostly saprobes, mucor, rhizopus may take for how many days

<5 DAY

Intermediate growers which are dermatophytes may take for how many days

6-10 DAY

Slow growers which are systemic fungi like histoplasma and blastomycosis may take for how many days

>11 DAY, 4-6 WEEK

Macroscopic features which examined on both obverse and reverse of the colony

PIGMENTATION

Pigmentation which shows front surface colour and texture

OBVERSE

Pigmentation which shows diffusable metabolite

REVERSE

This fungi often display distinct obverse and reverse pigmentation

DERMATOPHYTE

This fungi display dark pigmentation

DEMATIACEOUS FUNGI

What colour do Microsporum canis pigmentation displays

YELLOW ORANGE

What colour do Trichophyton rubrum pigmentation displays

DEEP RED-REDDISH

What colour do dematiaceous fungi pigmentation displays

OLIVE GREEN, BROWN, BLACK

Non pigmented fungi which is a counterpart of dematiaceous fungi

HYALINE

Fungal texture

COTTONY

Fungal texture

VELVETY

Fungal texture

GRANULAR

Fungal texture

GLABROUS

Macroscopic appearance of fungal colony type and amount of aerial hyphae fungal production

TEXTURE

Leathery/waxy and smooth with minimal mycelium

GLABROUS

Example of fungi which displays glabrous texture

DERMATOPHYTE, TRICHOPHYTON

Short aerial hyphae with soft surface

VELVETY, SUEDE LIKE

What fungi exhibits a velvety texture

ASPERGILLUS, PENICILLIUM

Resembles bacterial colonies, smooth with no aerial mycelium

YEAST LIKE

What fungi exhibits yeast like texture

CANDIDA ALBICAN

Long aerial hyphae with fluffy surface that could lift the lid

COTTONY, WOOLLY, FLOCCOSE

Heavy sporulation, rough, powdery, dry and abundant seen in condia spores

GRANULAR, POWDERY

What fungi exhibits granular and powdery texture

MICROSPORUM, TRICHOPHYTON

What macroscopic characteristics observed in SDA and PDA plates

TOPOGRAPHY

Smooth and even surface topography

FLAT

Radial grooves like a bicycle wheel topography

RUGOSE

Ridges arranged in folds with wrinkles, uneven surface seen in older mature colony topography

FOLDED

Central depression volcano like topography

CRATERIFORM

Rough wart like projections topography

VERRUCOSE

Convoluted folds resembling brain tissue topography

CEREBRIFORM

This macroscopic appearance shows early clue

GROWTH RATE

Fungi that are rapid growers

MUCOR

Fungi that slow growers

HISTOPLASMA

This features are combined for accurate fungal identification

MACROSCOPIC, MICROSCOPIC

Key notes for laboratory identification

GROWTH RATE, COLONY COLOR, TEXTURE, TOPOGRAPHY

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