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1
It is the simplest most common and best embedding medium which doesn't distort even it is immersed not more than 4 hours or use in serial sectioning
PARAFFIN WAX
2
What is the most common melting point of paraffin wax
56-58
3
Paraffin wax needed to be pure and filtered before use, what kind of filter should be used
COARSE FILTER PAPER
4
How many times the maximum reuse of paraffin wax
2
5
If paraffin wax was heated to about 100 to 105° c what substance or element trying to be removed
WATER
6
Paraffin wax should not be used with this kind of tissues as it can melt it out
FATTY TISSUE
7
If fixed knife microtome is used what kind of paraffin wax should be used
HIGH MELTING POINT
8
What is added on the paraffin to become paraplast
SYNTHETIC PLASTIC POLYMER
9
Paraplast has a melting point of what temperature
56-57
10
This is good for bones and brain and better in ribboning sections as it does not deposits on the slide after staining and requires no station processing schedule
PARAPLAST
11
This is the substitute similar with paraplast which has the same melting point which is less brittle and less compressible than paraplast
EMBEDDOL
12
This is used for embedding the eyes and womb
BIOLOID
13
Same as paraplast but it contains rubber
TISSUE MAT
14
It has low melting point but it is harder than paraffin and it is not soluble in water but only in 95% ethanol and clearing agents
ESTER WAX
15
This medium is used for enzyme histochemical studies and demonstration of neutral and lipids but it is hydroscopic which attracts water
CARBOWAX
16
Carbowax requires how many changes
4
17
What solution do we use in floating tissue ribbons
BLANK AND MCCARTHY SOLUTION, PEARSE SOLUTION
18
The most common adhesive for ribbon to be adhered on the slide
EGG ALBUMIN
19
This is added to the water for floatation when carbowax is used
10% POLYETHYLENE GLYCOL, SOAP
20
Celloidin is a purified form of what compound
NITROCELLULOSE
21
This medium is used for hollow cavities which tend to collapse like eyes and this is also used for hard tissues like bones and teeth and can also be used in whole embryo
CELLOIDIN
22
What are the percentages requirement for celloidin according to the thickness of the following tissues Thin- Medium- Thick-
2-4, 4-6, 8-12
23
This medium can be used for neurological tissues and it reduces distortion in sectioning due to its robbery consistency it also requires no heat
CELLOIDIN
24
This medium is very volatile and should be stored in 70 to 80% alcohol. Photo micrographs is also difficult to prepare in this medium
CELLOIDIN
25
This is highly flammable and it used in gun cotton and gunpowder as propellant for firearms
NITROCELLULOSE
26
What method of celloidine impregnation for bones teeth and brain and whole organs which requires soaking in 70 to 80% alcohol until ready for cutting
WET
27
What method in celloidine impregnation which is for wholel eyes and requires gilson's mixture which is chloroform plus cedar wood oil but does not need to be stored in alcohol
DRY
28
This medium can able to tolerate water and more easily to torn than celloidin however it is more explosive than celloidin. It would explode if hit even dry and exposure to direct sunlight should be avoided. This requires special equipment and non-conventional microtomy techniques which is not suitable for clinical histology laboratories
LOW VISCOSITY NITROCELLULOSE
29
This medium is used for light microscopic studies and for high resolution microscopy of tissues that sections which requires thinner than usual about 4 to 6 um and can be used also for renal biopsies and bone marrow biopsies
PLASTIC RESIN
30
Classification of plastic resins according to their chemical composition
EPOXY, POLYESTER, ACRYLIC
31
This is use for ultra structural studies and permits section as thin as 30 to 40 mm to be cut since it is stable in an electron beam
EPOXY
32
This reduces antigenicity but it is but it is sensitive on skin contact and inhalation
EPOXY
33
This is the toxic component of epoxy
VINYLCYCLOHEXANE DIOXIDE
34
This epoxy is the slowest infiltration
BISPHENOL A
35
This epoxy is the fastest infiltration
CYCLOHEXENE DIOXIDE
36
This type of plastic resin medium is rarely used and but it is used for em however epoxide are more superior
POLYESTER
37
It is a type of acrylic plastic resin medium used for light microscopy due to hydrophilic nature
POLYGLYCOL METHACRYLATE
38
It is a type of acrylic plastic racine used for undicalsified bone and other hard tissues due to hardness
METHYL METHACRYLATE
39
Medium used when dehydration is avoided or when tissues is subjected to histochemical and enzyme studies
GELATIN IMPREGNATION
40
It is a medium used for delicate specimens like friable, necrotic tissues and frozen tissues sections
GELATIN IMPREGNATION
41
In gelatin impregnation, the whole organs are subject to what kind of technique
GOUGH WENWORTH TECHNIQUE
42
When washing out the fixatives using the gelatin infiltration, the tissues are placed with these concentrations
1% PHENOL, 20% GELATIN
43
This prevents the growth of mold in gelatin infiltration
1% PHENOL
44
Preferred fixative for gelatin infiltration for 12-24 hours
10% FORMALIN
45
It is mainly used in double embedding as this medium do not prove sufficient support. They are used for FNAB specimens and small friable pieces
AGAR IMPREGNATION
46
The immortalisation of tissues presentation
EMBEDDING
47
Minimum allowance of surrounding margin of wax
2
48
This is the marker used and putted on the side of the tissue opposite that to be cut during grossing
INDIA INK
49
This must always be read during embedding for proper orientation
REQUISITION FORM
50
What ink should be used on margins of the tissue
INDIA INK
51
These type of tissues are placed diagonally
ELONGATED
52
Tubular tissues like the arteries, vein, fallopian tubes and GITs should be embedded in what perspective
CROSS SECTION
53
Skin biopsies like shave and punch must be oriented in what perspective
SIDE
54
Multiple tissues pieces are aligned in what perspective in the center of the mold
ACROSS THE LONG AXIS
55
Membrane specimens should be oriented in what perspective
SWISS ROLE
56
Endometrial curettings specimen should be oriented in what perspective
KEEP IN CENTER
57
An embedding mold with an L shaped metal plate
LEUCKHART'S EMBEDDING MOLD
58
The methods wherein tissues are first infiltrated w/CELLOIDIN and subsequently embedded in paraffin mass. This is used to facilitate cutting of large blocks of dense firm tissues like brain recommended for making small sections of celloidin blocks
DOUBLE EMBEDDING
59
The process of removing paraffin to form a 4 sided prism
TRIMMING
60
Embedding medium used for cutting into hard tissue like bones and teeth as well as for neurological tissues it has a robbery consistency and reduces distortion in sectioning and it also requires no heat
CELLOIDIN
61
This is also known as the epon epoxy
GLYCEROL
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