Infiltrating and embedding media MCQ

61問 • 1年前

問題一覧

It is the simplest most common and best embedding medium which doesn't distort even it is immersed not more than 4 hours or use in serial sectioning

PARAFFIN WAX

What is the most common melting point of paraffin wax

56-58

Paraffin wax needed to be pure and filtered before use, what kind of filter should be used

COARSE FILTER PAPER

How many times the maximum reuse of paraffin wax

If paraffin wax was heated to about 100 to 105° c what substance or element trying to be removed

WATER

Paraffin wax should not be used with this kind of tissues as it can melt it out

FATTY TISSUE

If fixed knife microtome is used what kind of paraffin wax should be used

HIGH MELTING POINT

What is added on the paraffin to become paraplast

SYNTHETIC PLASTIC POLYMER

Paraplast has a melting point of what temperature

56-57

This is good for bones and brain and better in ribboning sections as it does not deposits on the slide after staining and requires no station processing schedule

PARAPLAST

This is the substitute similar with paraplast which has the same melting point which is less brittle and less compressible than paraplast

EMBEDDOL

This is used for embedding the eyes and womb

BIOLOID

Same as paraplast but it contains rubber

TISSUE MAT

It has low melting point but it is harder than paraffin and it is not soluble in water but only in 95% ethanol and clearing agents

ESTER WAX

This medium is used for enzyme histochemical studies and demonstration of neutral and lipids but it is hydroscopic which attracts water

CARBOWAX

Carbowax requires how many changes

What solution do we use in floating tissue ribbons

BLANK AND MCCARTHY SOLUTION, PEARSE SOLUTION

The most common adhesive for ribbon to be adhered on the slide

EGG ALBUMIN

This is added to the water for floatation when carbowax is used

10% POLYETHYLENE GLYCOL, SOAP

Celloidin is a purified form of what compound

NITROCELLULOSE

This medium is used for hollow cavities which tend to collapse like eyes and this is also used for hard tissues like bones and teeth and can also be used in whole embryo

CELLOIDIN

What are the percentages requirement for celloidin according to the thickness of the following tissues Thin- Medium- Thick-

2-4, 4-6, 8-12

This medium can be used for neurological tissues and it reduces distortion in sectioning due to its robbery consistency it also requires no heat

CELLOIDIN

This medium is very volatile and should be stored in 70 to 80% alcohol. Photo micrographs is also difficult to prepare in this medium

CELLOIDIN

This is highly flammable and it used in gun cotton and gunpowder as propellant for firearms

NITROCELLULOSE

What method of celloidine impregnation for bones teeth and brain and whole organs which requires soaking in 70 to 80% alcohol until ready for cutting

WET

What method in celloidine impregnation which is for wholel eyes and requires gilson's mixture which is chloroform plus cedar wood oil but does not need to be stored in alcohol

DRY

This medium can able to tolerate water and more easily to torn than celloidin however it is more explosive than celloidin. It would explode if hit even dry and exposure to direct sunlight should be avoided. This requires special equipment and non-conventional microtomy techniques which is not suitable for clinical histology laboratories

LOW VISCOSITY NITROCELLULOSE

This medium is used for light microscopic studies and for high resolution microscopy of tissues that sections which requires thinner than usual about 4 to 6 um and can be used also for renal biopsies and bone marrow biopsies

PLASTIC RESIN

Classification of plastic resins according to their chemical composition

EPOXY, POLYESTER, ACRYLIC

This is use for ultra structural studies and permits section as thin as 30 to 40 mm to be cut since it is stable in an electron beam

EPOXY

This reduces antigenicity but it is but it is sensitive on skin contact and inhalation

EPOXY

This is the toxic component of epoxy

VINYLCYCLOHEXANE DIOXIDE

This epoxy is the slowest infiltration

BISPHENOL A

This epoxy is the fastest infiltration

CYCLOHEXENE DIOXIDE

This type of plastic resin medium is rarely used and but it is used for em however epoxide are more superior

POLYESTER

It is a type of acrylic plastic resin medium used for light microscopy due to hydrophilic nature

POLYGLYCOL METHACRYLATE

It is a type of acrylic plastic racine used for undicalsified bone and other hard tissues due to hardness

METHYL METHACRYLATE

Medium used when dehydration is avoided or when tissues is subjected to histochemical and enzyme studies

GELATIN IMPREGNATION

It is a medium used for delicate specimens like friable, necrotic tissues and frozen tissues sections

GELATIN IMPREGNATION

In gelatin impregnation, the whole organs are subject to what kind of technique

GOUGH WENWORTH TECHNIQUE

When washing out the fixatives using the gelatin infiltration, the tissues are placed with these concentrations

1% PHENOL, 20% GELATIN

This prevents the growth of mold in gelatin infiltration

1% PHENOL

Preferred fixative for gelatin infiltration for 12-24 hours

10% FORMALIN

It is mainly used in double embedding as this medium do not prove sufficient support. They are used for FNAB specimens and small friable pieces

AGAR IMPREGNATION

The immortalisation of tissues presentation

EMBEDDING

Minimum allowance of surrounding margin of wax

This is the marker used and putted on the side of the tissue opposite that to be cut during grossing

INDIA INK

This must always be read during embedding for proper orientation

REQUISITION FORM

What ink should be used on margins of the tissue

INDIA INK

These type of tissues are placed diagonally

ELONGATED

Tubular tissues like the arteries, vein, fallopian tubes and GITs should be embedded in what perspective

CROSS SECTION

Skin biopsies like shave and punch must be oriented in what perspective

SIDE

Multiple tissues pieces are aligned in what perspective in the center of the mold

ACROSS THE LONG AXIS

Membrane specimens should be oriented in what perspective

SWISS ROLE

Endometrial curettings specimen should be oriented in what perspective

KEEP IN CENTER

An embedding mold with an L shaped metal plate

LEUCKHART'S EMBEDDING MOLD

The methods wherein tissues are first infiltrated w/CELLOIDIN and subsequently embedded in paraffin mass. This is used to facilitate cutting of large blocks of dense firm tissues like brain recommended for making small sections of celloidin blocks

DOUBLE EMBEDDING

The process of removing paraffin to form a 4 sided prism

TRIMMING

Embedding medium used for cutting into hard tissue like bones and teeth as well as for neurological tissues it has a robbery consistency and reduces distortion in sectioning and it also requires no heat

CELLOIDIN

This is also known as the epon epoxy

GLYCEROL

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Jef Marc Valencia · 73問 · 2年前

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