LABELLED IMMUNOASSAY

Jef Marc Valencia · 73問 · 2年前

通報

問題一覧

What is the basis of the labeled immunoassays test

PRECIPITATION, AGGLUTINATION

Design for antigens and antibodies that may be small in size or present in very low concentrations

IMMUNOASSAY

The presence of such antigens are antibodies is determined indirectly by using a_ to detect whether or not specific binding has taken place

LABELLED REACTANT

Labels used in immunoassays

FLUORESCENT, RADIONUCLIDE, ENZYME, FREE RADICAL, FERRITIN

Immunoassays have always the same principle two different methods the only thing that differ is

LABEL

Labels used in fluorescence immunoassay

FLUOR, FLUOROCHROME

Labels used in radioimmunoassay

RADIONUCLIDE, RADIOISOTOPE

Labels used in chemiillumiscense

FREE RADICAL, FERRITIN

This test have the ability to absorb light at shorter wavelengths and anything light waves at a visible spectrum or a longer wavelengths

FLUORESCENCE SEROLOGY

Shorter wavelengths are also known as

ULTRAVIOLET LIGHT

Visible spectrum or longer wavelengths are also known as

INFRARED LIGHT

This test is covalently linked to immunoglobulin or to anti-immunoglobulin to detect the presence of ag or ab

FLUORESCENCE SEROLOGY

The most common type of fluorescent serology which emits fluorescence color green

FLUORESCEIN ISOTHIOCYANATE

Type of fluorescent serology which emits fluorescence color red

TETRAMETHYL RHODAMINE

A type of fluorescence immunoassay which can only be reported in positive or negative result

QUALITATIVE

What are the types of qualitative fluorescence immunoassay

DIRECT, INDIRECT, INHIBITION, COMPLEMENT STAINING

What are the types of fluorescence Immunoassay

QUALITATIVE, HOMOGENOUS, HETEROGENOUS

What is unknown in Direct Immunoflourescence

ANTIGEN

It is best suited to ag detection in tissue or body fluids

DIRECT

When agab is especialized by adhering into a microtiter plate

SOLID PHASE

Knowing the reagent of a test is known when it is

LABELED

A direct immunoflourescence technique used for the dtection of treponema pallidum

DARK FIELD MICROSCOPY

Typically visualized as bright apple green or orange yellow object against a dark background

ANTIGEN

It is used for the detection of treponema in indirect immunofluorescence

FLUORESCENCE TREPONEMAL ANTIBODY ABSORPTION TEST

This remove unattached ab and prevents false positive result

WASHING

The second reagent used in indirect immune fluorescence to induce a positive reaction by attaching it into the fc portion of the antibody

LABELED ANTI IMMUNOGLOBULIN

What type of test do Dark field Microscopy for treponema pallidum test used for the detection of treponema pallidum

TREPONEMA SEROLOGICAL TEST

In immunoassays this method is to remove unbound globulins

WASHING

This refers to the antibodies that is not attached to the antigen

UNBOUND GLOBULIN

What is the color emitted by the spirochetes in dark field microscopy

GREEN

What is the unknown in Indirect IF

ANTIBODY

What is the first reagent in inhibition immunofluorescence

ANTIGEN

What is the second reagent in inhibition immunofluorescence

LABELED ANTIBODY

An Indirect IF test used for the detection of treponema pallidum

TREPONEMAL ANTIBODY ABSORPTION TEST

Immunoglobulins capable of fixing complement

IGM, IGG

What is the test shown

DIRECT

What is the test shown

INDIRECT

A qualitative fluorescence immunoasing which the patient serum and complement is mixed with reagent antigen fixed on the slide

COMPLEMENT STAINING

A blocking test in which an antigen is exposed to unlabelled anitbody to labelled antibody and washed

INHIBITION IMMUNOFLUORESCENCE

FPIA has the same principle with this test in agglutination

NEPHELOMETRY

What is the unknown in Inhibition IF

ANTIBODY

If unlabeled and labeled antibodies are both_ to antigen there is no fluorescence

HOMOLOGOUS

Homogenous fluorescence immunoassay is also known as

FLUORESCENCE POLARIZATION IMMUNOASSAY

This remove unbound globulins preventing false negative result in Inhibition IF

WASHING

In FPIA, incident light directed at the specimen is polarized with a lens or prism so the waves are aligned in one plane. If a molecule is small and rotates quickly enough the emitted light is_resulting in what result

UNPOLARIZED, NEGATIVE

Reagent used to washed off labelled reagent

NORMAL SALINE

What is the unknown in Complement Staining IF

ANTIBODY

However the labeled molecule is bound to antibody the molecule is unable tumble rapidly and it emits an_resulting in what result

POLARIZED, POSITIVE

A type of fluorescence immunoassay which uses polyacrylamide beads coated with anti-globulin or antibodies that has been developed.

HETEROGENOUS, SOLID PHASE FLUORESCENCE IMMUNOASSAY

A type Fluorescence Immunoassay that requires no separation procedure and also rapid and simpler to perform

HOMOGENOUS

Fluorescence Immunoassay that is based on the change in polarization of fluorescent light emitted from a labelled molecule when it is bound by antibody

FLOURESCENCE POLARIZATION IMMUNOASSAY

Solid phase fluorescence immunoassay is utilized for the diagnosis of

FIXED VIRAL INFECTION

This is the oldest and most sensitive immunoassay

RADIOIMMUNOASSAY

Who discovered radioimmunoassay

ROSALYN YALOW

Enumerate the uses of radioimmunoassay

MONITORING LEVEL OF HORMONE, DETECTION OF VITAMIN, DETECTION OF VIRAL ANTIGEN, DETECTION OF THERAPEUTIC AND ABUSED DRUG

Most commonly used radioactive label

GAMMA EMITTER

What are the radioactive label used in radio immunoassay

GAMMA EMITTER, BETA EMITTER

What are the gamma emitters use as a radioactive label

IODINE 125, IODINE 131

What are the beta emitter used as radioactive label

TRITIUM

What machine is used to measure the gamma emitter

SOLID CRYSTAL SCINTILLATION COUNTER

What machine is used to measure the beta emitter

LIQUID SCINTILLATION COUNTER

What are the different types of radioimmunoassay

CLASSIC COMPETITIVE ANALYSIS, RADIO IMMUNOMETRIC ASSAY, RADIO IMMUNOSORBENT ASSAY

Classic radio immunoassay competitive analysis is also known as

DISPLACEMENT/RADIOLIGAND INHIBITION

Classic RIA First reagent: Unknown: Second reagent: Principle: Result:

SOLID PHASE ANTIBODY, ANTIGEN, LABELLED ANTIGEN, COMPETITIVE BINDING ASSAY, INVERSELY PROPORTIONAL

This refers to the two analytes that compete for binding site

COMPETITIVE BINDING

Which of the following is positive result of Classic RIA

Radioactivity: Px Ag:

INCREASE, DECREASE

Radioactivity: Px Ag:

DECREASE, INCREASE

Non-competitive RIA is also known as

IMMUNOMETRICRADIO ASSAY

Immunoradiometric Assay First reagent: Unknown: Second reagent: Principle: Result:

SOLID PHASE ANTIGEN, ANTIBODY, LABELED RADIOISOTOPE ANTI IMMUNOGLOBULIN, NON-COMPETITIVE BINDING ASSAY, DIRECTLY PROPORTIONAL

Identify the type of IMMUNOASSAY below

IMMUNOMETRICRADIO ASSAY

In IRMA, second antibody which is labeled radioisotope is added will bind to what portion of the immunoglobulin

FC PORTION

Indirect Radioimmunosorbent Assay First reagent: Unknown: Second reagent: Principle: Result:

ANTI IGE, PATIENT IGE, RADIOLABELED IGE, COMPETITIVE BINDING ASSAY, INDIRECTLY PROPORTIONAL

In indirect radioimmunosorbent assay, patient ige competes with radio labeled ige for the ige that is bound in

SOLID PHASE

Competition site of the classic RIA

PARATOPE

Radio immunosorbent test is also known as

DIRECT NON-COMPETITIVE BINDING RADIOIMMUNOSORBENT

RIST First reagent: Unknown: Second reagent: Principle: Result:

ANTI IGE, PATIENT IGE, RADIOLABELED IGE, NON-COMPETITIVE BINDING ASSAY, DIRECTLY PROPORTIONAL

RIP Transcribe: First reagent: Unknown: Second reagent: Principle: Result:

RADIOPRECIPITATION TEST, ANTI HUMAN IGE, PATIENT IGE, RADIOLABELED IGE, COMPETITIVE BINDING ASSAY, INVERSELY PROPORTIONAL

RAST Transcribe: First reagent: Unknown: Second reagent: Principle: Result:

RADIOALLERGOSORBENT TEST, ANTI IGE, PATIENT IGE, RADIOLABELED IGE, NON-COMPETITIVE BINDING ASSAY, DIRECTLY PROPORTIONAL

This RIA measures the total ige

RIP

This RIA measures the specific ige

RAST

COMPH Lesson 5

COMPH Lesson 5

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NPN

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NPN 2

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LESSON 10

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Introduction Page 2

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Introduction pg 3

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meet 2

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dis 1

dis 1

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dis 4

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dis 5

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Introduction

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