Chapter 6

Chapter 6
67問 • 1年前
  • eiren
  • 通報

    問題一覧

  • 1

    Low O2 and high CO2 concentrations mimic conditions found in the

    Intestinal tract

  • 2

    Candle jars increase CO2 concentrations and leave some O2 for ___ capnophiles

    Aerobic

  • 3

    CO2 incubators or candle jars are used to grow bacteria requiring an increased ___ concentration

    CO2

  • 4

    Capnophiles are

    Bacteria requiring an increased CO2 concentration

  • 5

    Selective media inhibits growth of unwanted organisms via

    All

  • 6

    Selective media allows for growth of only

    Desired microbes

  • 7

    Different ___ are used to distinguish among different organisms

    Media

  • 8

    Blood agar is used to identify organisms that ___ red blood cells

    Lyse

  • 9

    Selective and different media may be combined

    True

  • 10

    A ph indicator changes color if

    Mannitol is fermented to acid

  • 11

    Some gram negative bacteria can't grow on lactose

    True

  • 12

    ___ is used to encourage the growth of a particular microorganism in a mixed culture

    Enrichment culture

  • 13

    ___ is a visible mass of microbial cells that theoretically arose from one cell

    A colony

  • 14

    Pure cultures are usually obtained by the

    Streak plate method

  • 15

    Microbes an be preserved for a long period of time via

    Deep-freezing or lyophilization

  • 16

    Define lyophilization

    A quick freeze in which a vacuum pump removes water in a process called sublimation

  • 17

    The normal reproductive method of bacteria is

    Binary fission

  • 18

    Binary fission

    A single cell divides into two identical cells

  • 19

    Some bacteria reproduce by

    All

  • 20

    The time required for a cell to divide or a population to double is known as ___

    The generation time

  • 21

    Most bacteria have a doubling time of

    1-3 hours

  • 22

    Bacterial division occurs according to a ___

    Logarithmic progression

  • 23

    A chemically defined medium is one in which the exact is ___

    Known

  • 24

    Broth is good for growing ___

    Large batches of organisms

  • 25

    Any material prepared for the growth of bacteria in a laboratory

    Culture medium

  • 26

    Psychrophiles

    Cold-loving

  • 27

    Mesophiles

    Moderate-temperature loving

  • 28

    Thermophiles

    Heat -loving

  • 29

    The requirements for microbial growth are ___

    Physical and chemical

  • 30

    Most bacteria grow best at a pH value between ___

    6.5 and 7.5

  • 31

    Bacteria known as ___ may grow in pH conditions as low as 1

    Acidophiles

  • 32

    In a hypertonic solution most microbes undergo ___

    Plasmolysis

  • 33

    ___ can tolerate high salt concentrations

    Halophiles

  • 34

    All organisms require a ___

    Carbon source

  • 35

    What is needed for protein and nucleic acid synthesis?

    Nitrogen

  • 36

    Nitrogen is required for protein and nucleic acid synthesis. Where can nitrogen be obtained?

    The decomposition of proteins

  • 37

    Facultative anaerobes grow better in ___

    Aerobic conditions

  • 38

    Facultative anaerobes can grow in ___ conditions

    Aerobic or anaerobic

  • 39

    E. coli are considered facultative aerobes

    True

  • 40

    ___ don't use 02 but tolerate it

    Aerotolerant anaerobes

  • 41

    Many ___ ferment carbohydrate to lactate

    Aerotolerant anaerobes

  • 42

    Aerotolerant anaerobes fermenting carbohydrate to lactate will ___ the growth of aerobes

    Inhibit

  • 43

    ___ are sensitive to oxygen-derived free radicals

    Microaerophilic aerobes

  • 44

    Only aerobic growth; oxygen required

    Obligate aerobes

  • 45

    Common solidifying agent for a culture medium

    Agar

  • 46

    The exact chemical composition varies batch to batch

    Complex medium

  • 47

    A bacterial respiratory enzyme that can be put in media

    Oxyrase

  • 48

    Some parasitic and fastidious bacteria must be cultured in living animals or cell walls because

    Won't grow on artificial media

  • 49

    Media type for growth of chemoautotrophs and photoautotrophs

    Chemically defined

  • 50

    Media type for growth of most chemoorganismsheterotrophic

    Complex

  • 51

    Media type for growth of obligate anaerobes

    Reducing

  • 52

    Media type for suppression of unwanted microbes; encouraging desired microbes

    Selective

  • 53

    Media type for differentiation of colonies of desired microbes from others

    Differential

  • 54

    Media type designed to increase numbers of desired microbes to detectable levels

    Enrichment

  • 55

    During this phase of growth there is little to no change in the number of cells but metabolic activity is high

    Lag phase

  • 56

    During this growth phase the bacteria multiply at the fastest rate possible under conditions provided

    Log phase

  • 57

    During this phase of growth there is an equilibrium between cell division and death

    Stationary phase

  • 58

    During this phase of growth the number of deaths exceeds the number of cells formed

    Death phase

  • 59

    DNA and enzyme synthesis occurs during the ___ phase of growth

    Lag

  • 60

    pH increases as nutrients are exhausted and waste products build up during the ___ phase

    Death phase

  • 61

    Why are plate counts reported as the number of colony forming units (CFU) instead of the number of cells?

    Because its impossible to say that each colony actually arose from an individual cell

  • 62

    What happens if the concentration of bacteria on a plate is too great?

    The colonies will grow into each other and the plate will be uncountable

  • 63

    To ensure a countable plate a series of ___ should be plated

    Dilutions

  • 64

    In ___ bacteria are retained on the surface of a membrane filter and then transferred to a culture medium to grow and subsequently be counted

    Filtration

  • 65

    What is used to determine turbidity by measuring the amount of light that passed through a suspension of cells?

    Spectophotrometer

  • 66

    An indirect way of estimating bacterial numbers is ___

    Measuring the metabolic activity of the population

  • 67

    What is a convenient method of growth measurement for filamentous organisms such as fungi?

    Measuring dry weight

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    問題一覧

  • 1

    Low O2 and high CO2 concentrations mimic conditions found in the

    Intestinal tract

  • 2

    Candle jars increase CO2 concentrations and leave some O2 for ___ capnophiles

    Aerobic

  • 3

    CO2 incubators or candle jars are used to grow bacteria requiring an increased ___ concentration

    CO2

  • 4

    Capnophiles are

    Bacteria requiring an increased CO2 concentration

  • 5

    Selective media inhibits growth of unwanted organisms via

    All

  • 6

    Selective media allows for growth of only

    Desired microbes

  • 7

    Different ___ are used to distinguish among different organisms

    Media

  • 8

    Blood agar is used to identify organisms that ___ red blood cells

    Lyse

  • 9

    Selective and different media may be combined

    True

  • 10

    A ph indicator changes color if

    Mannitol is fermented to acid

  • 11

    Some gram negative bacteria can't grow on lactose

    True

  • 12

    ___ is used to encourage the growth of a particular microorganism in a mixed culture

    Enrichment culture

  • 13

    ___ is a visible mass of microbial cells that theoretically arose from one cell

    A colony

  • 14

    Pure cultures are usually obtained by the

    Streak plate method

  • 15

    Microbes an be preserved for a long period of time via

    Deep-freezing or lyophilization

  • 16

    Define lyophilization

    A quick freeze in which a vacuum pump removes water in a process called sublimation

  • 17

    The normal reproductive method of bacteria is

    Binary fission

  • 18

    Binary fission

    A single cell divides into two identical cells

  • 19

    Some bacteria reproduce by

    All

  • 20

    The time required for a cell to divide or a population to double is known as ___

    The generation time

  • 21

    Most bacteria have a doubling time of

    1-3 hours

  • 22

    Bacterial division occurs according to a ___

    Logarithmic progression

  • 23

    A chemically defined medium is one in which the exact is ___

    Known

  • 24

    Broth is good for growing ___

    Large batches of organisms

  • 25

    Any material prepared for the growth of bacteria in a laboratory

    Culture medium

  • 26

    Psychrophiles

    Cold-loving

  • 27

    Mesophiles

    Moderate-temperature loving

  • 28

    Thermophiles

    Heat -loving

  • 29

    The requirements for microbial growth are ___

    Physical and chemical

  • 30

    Most bacteria grow best at a pH value between ___

    6.5 and 7.5

  • 31

    Bacteria known as ___ may grow in pH conditions as low as 1

    Acidophiles

  • 32

    In a hypertonic solution most microbes undergo ___

    Plasmolysis

  • 33

    ___ can tolerate high salt concentrations

    Halophiles

  • 34

    All organisms require a ___

    Carbon source

  • 35

    What is needed for protein and nucleic acid synthesis?

    Nitrogen

  • 36

    Nitrogen is required for protein and nucleic acid synthesis. Where can nitrogen be obtained?

    The decomposition of proteins

  • 37

    Facultative anaerobes grow better in ___

    Aerobic conditions

  • 38

    Facultative anaerobes can grow in ___ conditions

    Aerobic or anaerobic

  • 39

    E. coli are considered facultative aerobes

    True

  • 40

    ___ don't use 02 but tolerate it

    Aerotolerant anaerobes

  • 41

    Many ___ ferment carbohydrate to lactate

    Aerotolerant anaerobes

  • 42

    Aerotolerant anaerobes fermenting carbohydrate to lactate will ___ the growth of aerobes

    Inhibit

  • 43

    ___ are sensitive to oxygen-derived free radicals

    Microaerophilic aerobes

  • 44

    Only aerobic growth; oxygen required

    Obligate aerobes

  • 45

    Common solidifying agent for a culture medium

    Agar

  • 46

    The exact chemical composition varies batch to batch

    Complex medium

  • 47

    A bacterial respiratory enzyme that can be put in media

    Oxyrase

  • 48

    Some parasitic and fastidious bacteria must be cultured in living animals or cell walls because

    Won't grow on artificial media

  • 49

    Media type for growth of chemoautotrophs and photoautotrophs

    Chemically defined

  • 50

    Media type for growth of most chemoorganismsheterotrophic

    Complex

  • 51

    Media type for growth of obligate anaerobes

    Reducing

  • 52

    Media type for suppression of unwanted microbes; encouraging desired microbes

    Selective

  • 53

    Media type for differentiation of colonies of desired microbes from others

    Differential

  • 54

    Media type designed to increase numbers of desired microbes to detectable levels

    Enrichment

  • 55

    During this phase of growth there is little to no change in the number of cells but metabolic activity is high

    Lag phase

  • 56

    During this growth phase the bacteria multiply at the fastest rate possible under conditions provided

    Log phase

  • 57

    During this phase of growth there is an equilibrium between cell division and death

    Stationary phase

  • 58

    During this phase of growth the number of deaths exceeds the number of cells formed

    Death phase

  • 59

    DNA and enzyme synthesis occurs during the ___ phase of growth

    Lag

  • 60

    pH increases as nutrients are exhausted and waste products build up during the ___ phase

    Death phase

  • 61

    Why are plate counts reported as the number of colony forming units (CFU) instead of the number of cells?

    Because its impossible to say that each colony actually arose from an individual cell

  • 62

    What happens if the concentration of bacteria on a plate is too great?

    The colonies will grow into each other and the plate will be uncountable

  • 63

    To ensure a countable plate a series of ___ should be plated

    Dilutions

  • 64

    In ___ bacteria are retained on the surface of a membrane filter and then transferred to a culture medium to grow and subsequently be counted

    Filtration

  • 65

    What is used to determine turbidity by measuring the amount of light that passed through a suspension of cells?

    Spectophotrometer

  • 66

    An indirect way of estimating bacterial numbers is ___

    Measuring the metabolic activity of the population

  • 67

    What is a convenient method of growth measurement for filamentous organisms such as fungi?

    Measuring dry weight