protein analysis

26問 • 1年前

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In the earlier days, the complex nitrogen-rich substance found in cells of all animals and plants are not identified until Berzelius suggested the name?

protein

Who suggested the name protein?

berzelius

What is the meaning of greek work proteios?

primary

Most of the 20 basic amino acids present in proteins were present from?

1819 until 1904

Amino acid are the building blocks of the cell, which constitute majority of its dry mass

false (proteins)

Proteins are the products of nucleic acid transcription and translation, the ultimate impact of the data processed and produced by nucleic acid is expressed in proteins

true

In what year, Martin and Synge developed chromatography, a technique which is now widely used to separate proteins?

1942

Who developed chromatography?

Martin and Synge

What technique is now widely used to separate proteins that was developed by Martin and Synge in 1942?

chromatography

In protein isolation, the procedure that may be used to achieve optimal isolation of proteins/ Protein isolation procedures depends upon:

sample type, location of protein of interest, required protein yield for the subsequent analtsis to be performed

This is where you want to quantify or identify your desired proteins

western blotting, enzyme-linked immunosorbent (ELISA), gel shift assays (EMSA), reporter assays, mass spectrometry immunoprecipitation

this are the first phases in cell fractionation

tissue disruption and cell lysis

The goal is to disaggregate the cells and break them open with minimal damage to the cellular fraction of interest

lysing cells and tissues

What are/is the methods for lysing cells ranger from physical methods?

heat treatment, homogenization, sonication

What are/is the methods for lysing cells ranger from chemical methods?

treatment with a detergent solution

This technique of protein isolation by physical disruption depend on the use of rotating blades to grind and disperse substantial amounts of complex tissue.

mechanical methods

Waring blender and polytron are used in what techniques of protein isolation by disruption?

mechanical methods

This technique of protein isolation by physical disruption is the most commonly used technique for cell disruption.

liquid homogenization

AWhat type of homogenizer consists of a round glass pestle that is manually driven into a glass tube

dounce homogenizer

What type of homogenizer consists of manually or chemical driven polytetrafluoroethylene pestle shaped to fit a rounded or conical vessel

potter-elvehjem homoginizer

What type of homogenizer consists of piston that us used to apply high pressure to a sample volume 40-250 mL, forcing it through a tiny hole in the press.

french press

This equipment is expensive and is often the method of choice for breaking the bacterial cells mechanically

french press

This is commonly used to lyse bacterial and mammalian cells

freeze-thaw method

This is the most basic and common method used to disrupt cells

manual grinding

This is another method that can be used to physically disrupt and break open cells

sonication

This is a primary protein purification methis in use in most laboratories

column chromatography

airlaw and airworthyness

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airlaw and airworthyness