overview of PCR

36問 • 1年前

ユーザ名非公開

通報

問題一覧

What is the first specific amplification method of any type was?

Polymerase chain reaction (PCR)

What is the first and prototypical method for amplifying target nucleic acid?

Polymerase chain reaction (PCR)

Who developed PCR?

kary mullis mid-1980’s

This allows the rapid synthesis of designated fragments of DNA

PCR

The main goal of PCR is to create multiple copies/ amplify target (DNA/RNA) sequence

true

AKA “copy machine” for DNA that revolutionized molecular biology

PCR

Target amplification involves making MANY COPIES of a specific DNA sequence

amplicons

PCR is DNA replication in vitro

true

What is the main replication enzyme of PCR?

DNA polymerase

What are the components of PCR?

water, buffer, DNA template, Thermostable polymerase, primer pair, magnesium, deoxynucleotides triphosphates (dNTPs)

Components of PCR is also known as?

AOTA

Stabilizes the DNA polymerase, DNA and nucleotides

buffer

Containes the target region of interest

DNA template

What is the critical componen of PCR?

primer pair

Primer pair is also known as?

Oligodeoxynucleotides

This stabilized the DNA double helix in components of PCR

magnesium

Equimolar building blocks of DNA

dNTPs

Preparation of PCR reaction components (mastermix)

Pre PCR

Isolation of nucleic acid from samples

Pre PCR

Thermal cycling (denaturation, annealing, extension)

PCR

Analysis of amplified DNA

post PCR

What is seen in the picture?

typical PCR set-up in laboratory

High heat to disrupt and separated DNA duplexes

Heat denaturation (PCR)

Attachment of primers to the template strand

primer annealing (PCR)

Elongation of daughter strand with DNA polymerase

primer extension (PCR)

This control in PCR ensures that the ENZYME IS ACTIVE , the buffer is optimal, the primers are priming the right sequences, and the thermal cycler is cyling appropriately

positive control

Aka contamination control or reagent blank which ensures that the reaction MIX IS NOT CONTAMINATED WITH TEMPLATE DNA or amplified products from a previos run

negative control without DNA

Lacks the target sequence, ensures that the primers are NOT ANNEALING to nontarget sequences of DNA

negative control with DNA

RT stands for?

reverse transcription

rt stands for?

real time

This refers to the CONVERSION OF RNA TEMPLATE into its complementary DNA strand (cDNA)

Reverse transcriptase PCR

This is a POWERFUL TECHNIQUE that enables amplification of TWO or MORE products PARALLEL in a single reaction tube.

Multiplex PCR

What do you call this?

cold rack

What do you call this?

PCR strips

What do you call this?

PCR plates

What do you call this?

centrifuge

airlaw and airworthyness

ユーザ名非公開 · 12問 · 2年前

airlaw and airworthyness

12問 • 2年前

ユーザ名非公開

maintenance forms and records

ユーザ名非公開 · 36問 · 2年前

maintenance forms and records

36問 • 2年前

ユーザ名非公開

maintenance publications

ユーザ名非公開 · 38問 · 2年前

maintenance publications

38問 • 2年前

ユーザ名非公開

mechanic privileges and limitations

ユーザ名非公開 · 33問 · 2年前

mechanic privileges and limitations

pcar

pcar

AFCAR

AFCAR

HUMAN FACTOR

HUMAN FACTOR

CELL BLOCK

CELL BLOCK

Real time PCR or Quantitative PCR (qPCR)

ユーザ名非公開 · 23問 · 1年前

Real time PCR or Quantitative PCR (qPCR)

protein analysis

protein analysis

MECHANIC PRIVILEGES AND LIMITATIONS

ユーザ名非公開 · 34問 · 1年前

MECHANIC PRIVILEGES AND LIMITATIONS

CELL BLOCK

CELL BLOCK

CELL BLOCK

CELL BLOCK

AUTOPSY

AUTOPSY

Routine Tisse Processing

ユーザ名非公開 · 57問 · 1年前

Routine Tisse Processing

FILIPINO VOCABULARY

FILIPINO VOCABULARY

WEEK 3 MACROSCOPIC, DISEASE CORRELATION, URINE COLOR, URINE VOLUME, URINE TRANSPARENCY, AND URINE ODOR

ユーザ名非公開 · 52問 · 1年前

WEEK 3 MACROSCOPIC, DISEASE CORRELATION, URINE COLOR, URINE VOLUME, URINE TRANSPARENCY, AND URINE ODOR

52問 • 1年前

ユーザ名非公開

WEEK 1 INTRO, HISTORY AND RENAL ANATOMY

ユーザ名非公開 · 31問 · 1年前

WEEK 1 INTRO, HISTORY AND RENAL ANATOMY

CARBS

CARBS

問題一覧

What is the first specific amplification method of any type was?

Polymerase chain reaction (PCR)

What is the first and prototypical method for amplifying target nucleic acid?

Polymerase chain reaction (PCR)

Who developed PCR?

kary mullis mid-1980’s

This allows the rapid synthesis of designated fragments of DNA

PCR

The main goal of PCR is to create multiple copies/ amplify target (DNA/RNA) sequence

true

AKA “copy machine” for DNA that revolutionized molecular biology

PCR

Target amplification involves making MANY COPIES of a specific DNA sequence

amplicons

PCR is DNA replication in vitro

true

What is the main replication enzyme of PCR?

DNA polymerase

What are the components of PCR?

water, buffer, DNA template, Thermostable polymerase, primer pair, magnesium, deoxynucleotides triphosphates (dNTPs)

Components of PCR is also known as?

AOTA

Stabilizes the DNA polymerase, DNA and nucleotides

buffer

Containes the target region of interest

DNA template

What is the critical componen of PCR?

primer pair

Primer pair is also known as?

Oligodeoxynucleotides

This stabilized the DNA double helix in components of PCR

magnesium

Equimolar building blocks of DNA

dNTPs

Preparation of PCR reaction components (mastermix)

Pre PCR

Isolation of nucleic acid from samples

Pre PCR

Thermal cycling (denaturation, annealing, extension)

PCR

Analysis of amplified DNA

post PCR

What is seen in the picture?

typical PCR set-up in laboratory

High heat to disrupt and separated DNA duplexes

Heat denaturation (PCR)

Attachment of primers to the template strand

primer annealing (PCR)

Elongation of daughter strand with DNA polymerase

primer extension (PCR)

This control in PCR ensures that the ENZYME IS ACTIVE , the buffer is optimal, the primers are priming the right sequences, and the thermal cycler is cyling appropriately

positive control

Aka contamination control or reagent blank which ensures that the reaction MIX IS NOT CONTAMINATED WITH TEMPLATE DNA or amplified products from a previos run

negative control without DNA

Lacks the target sequence, ensures that the primers are NOT ANNEALING to nontarget sequences of DNA

negative control with DNA

RT stands for?

reverse transcription

rt stands for?

real time

This refers to the CONVERSION OF RNA TEMPLATE into its complementary DNA strand (cDNA)

Reverse transcriptase PCR

This is a POWERFUL TECHNIQUE that enables amplification of TWO or MORE products PARALLEL in a single reaction tube.

Multiplex PCR

What do you call this?

cold rack

What do you call this?

PCR strips

What do you call this?

PCR plates

What do you call this?

centrifuge