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Real time PCR or Quantitative PCR (qPCR)

Real time PCR or Quantitative PCR (qPCR)
23問 • 1年前
  • ユーザ名非公開
    • 通報

    問題一覧

  • 1

    This was discovered in reverse transcribing viruses

    Reverse transcription PCR

  • 2

    RNA- dependent DNA polymerases which bind to RNA templates and convert them to their complementary DNA sequence in the presence of dNTPs and other essential cofactors.

    Reverse transcripption PCR

  • 3

    Real time PCR is also known as?

    AOTA

  • 4

    Principe of traditional PCR is applied, but PCR products are detected at each cycle in real time as reaction progresses

    real time PCR

  • 5

    How many major phases are there in the PCR amplification curve?

    4

  • 6

    What are the 4 major phases in the PCR amplification curve?

    linear ground phase, early exponential phase, log-linear phase, plateau phase

  • 7

    Start of PCR, amplification is not detectable.

    linear ground phase

  • 8

    Exponential amplification becomes detectable

    early exponential phase

  • 9

    Reactions slows down due to depletion of reagent components and lower PCR efficiency

    log-linear phase

  • 10

    Reaction components become limited; reaction stops

    plateau phase

  • 11

    Quantitation of nucleic acid must be done at the log-linear phase

    false (early exponential phase)

  • 12

    Basal level of fluorescence defined during initial stage of PCR

    baseline

  • 13

    Point which significant and specific amplification occurs and fluorescence signal rises above backround level

    threshold

  • 14

    Threshold cyle, this is the number at which the fluorescence signal crosses the threshold

    C (t) or Ct

  • 15

    In threshold an C(t), the more initial concentration, the less the C(t) value

    true

  • 16

    Amount of target nucleic acid is reported as a copy number of concentration

    absolute quantification

  • 17

    Amount of target nucleic acid is reported comparative to the abundance of another gene

    relative quantification

  • 18

    Calculated from a standard curve as generated for absolute quantification

    amplification efficiencies

  • 19

    This is used to detect and quantify RNA. Total RNA or mRNA is first transcribed into complementary DNA (cDNA).

    Quantitative reverse transcription PCR (RT-qPCR)

  • 20

    This is used in a variety of applications including gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research

    Quantitative reverse transcription PCR (RT-qPCR)

  • 21

    This assay combine reverse transcription and PCR in a single tube and buffer, using a reverse transcriptase along with a DNA polymerase

    one-step assay RT-qPCR

  • 22

    This assay the reverse trancription and PCR steps are performed in separate tubes, with different optimized buffers, reaction conditions, and priming strategies

    two-step assay RT-qPCR

  • 23

    PCR is used to amplify DNA, while RT-PCR is used to amplify RNA

    True

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    問題一覧

  • 1

    This was discovered in reverse transcribing viruses

    Reverse transcription PCR

  • 2

    RNA- dependent DNA polymerases which bind to RNA templates and convert them to their complementary DNA sequence in the presence of dNTPs and other essential cofactors.

    Reverse transcripption PCR

  • 3

    Real time PCR is also known as?

    AOTA

  • 4

    Principe of traditional PCR is applied, but PCR products are detected at each cycle in real time as reaction progresses

    real time PCR

  • 5

    How many major phases are there in the PCR amplification curve?

    4

  • 6

    What are the 4 major phases in the PCR amplification curve?

    linear ground phase, early exponential phase, log-linear phase, plateau phase

  • 7

    Start of PCR, amplification is not detectable.

    linear ground phase

  • 8

    Exponential amplification becomes detectable

    early exponential phase

  • 9

    Reactions slows down due to depletion of reagent components and lower PCR efficiency

    log-linear phase

  • 10

    Reaction components become limited; reaction stops

    plateau phase

  • 11

    Quantitation of nucleic acid must be done at the log-linear phase

    false (early exponential phase)

  • 12

    Basal level of fluorescence defined during initial stage of PCR

    baseline

  • 13

    Point which significant and specific amplification occurs and fluorescence signal rises above backround level

    threshold

  • 14

    Threshold cyle, this is the number at which the fluorescence signal crosses the threshold

    C (t) or Ct

  • 15

    In threshold an C(t), the more initial concentration, the less the C(t) value

    true

  • 16

    Amount of target nucleic acid is reported as a copy number of concentration

    absolute quantification

  • 17

    Amount of target nucleic acid is reported comparative to the abundance of another gene

    relative quantification

  • 18

    Calculated from a standard curve as generated for absolute quantification

    amplification efficiencies

  • 19

    This is used to detect and quantify RNA. Total RNA or mRNA is first transcribed into complementary DNA (cDNA).

    Quantitative reverse transcription PCR (RT-qPCR)

  • 20

    This is used in a variety of applications including gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research

    Quantitative reverse transcription PCR (RT-qPCR)

  • 21

    This assay combine reverse transcription and PCR in a single tube and buffer, using a reverse transcriptase along with a DNA polymerase

    one-step assay RT-qPCR

  • 22

    This assay the reverse trancription and PCR steps are performed in separate tubes, with different optimized buffers, reaction conditions, and priming strategies

    two-step assay RT-qPCR

  • 23

    PCR is used to amplify DNA, while RT-PCR is used to amplify RNA

    True